This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.13 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 69,073 (2.6%) Reads that were too short: 16,058 (0.6%) Reads written (passing filters): 53,015 (2.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 12,378,418 bp (1.5%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 69073 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 153 661.3 0 153 7 358 661.3 0 358 8 18697 661.3 0 18697 9 69 661.3 0 69 10 29 661.3 0 29 11 34 661.3 0 34 12 20 661.3 0 20 13 49 661.3 0 49 14 19 661.3 0 19 15 25 661.3 0 25 16 45 661.3 0 45 17 34 661.3 0 34 18 55 661.3 0 55 19 49 661.3 0 49 20 45 661.3 0 45 21 25 661.3 0 25 22 23 661.3 0 23 23 51 661.3 0 51 24 44 661.3 0 44 25 49 661.3 0 49 26 42 661.3 0 42 27 48 661.3 0 48 28 157 661.3 0 157 29 185 661.3 0 185 30 513 661.3 0 513 31 377 661.3 0 377 32 6229 661.3 0 6229 33 62 661.3 0 62 34 23 661.3 0 23 35 23 661.3 0 23 36 29 661.3 0 29 37 25 661.3 0 25 38 22 661.3 0 22 39 21 661.3 0 21 40 29 661.3 0 29 41 14 661.3 0 14 42 23 661.3 0 23 43 31 661.3 0 31 44 40 661.3 0 40 45 69 661.3 0 69 46 133 661.3 0 133 47 289 661.3 0 289 48 19 661.3 0 19 49 18 661.3 0 18 50 6 661.3 0 6 51 21 661.3 0 21 52 15 661.3 0 15 53 15 661.3 0 15 54 23 661.3 0 23 55 8 661.3 0 8 56 15 661.3 0 15 57 18 661.3 0 18 58 24 661.3 0 24 59 19 661.3 0 19 60 26 661.3 0 26 61 28 661.3 0 28 62 10 661.3 0 10 63 26 661.3 0 26 64 16 661.3 0 16 65 15 661.3 0 15 66 9 661.3 0 9 67 15 661.3 0 15 68 12 661.3 0 12 69 65 661.3 0 65 70 33 661.3 0 33 71 94 661.3 0 94 72 102 661.3 0 102 73 63 661.3 0 63 74 80 661.3 0 80 75 135 661.3 0 135 76 232 661.3 0 232 77 310 661.3 0 310 78 169 661.3 0 169 79 116 661.3 0 116 80 168 661.3 0 168 81 152 661.3 0 152 82 287 661.3 0 287 83 378 661.3 0 378 84 402 661.3 0 402 85 238 661.3 0 238 86 138 661.3 0 138 87 71 661.3 0 71 88 89 661.3 0 89 89 231 661.3 0 231 90 218 661.3 0 218 91 547 661.3 0 547 92 1023 661.3 0 1023 93 299 661.3 0 299 94 153 661.3 0 153 95 476 661.3 0 476 96 455 661.3 0 455 97 352 661.3 0 352 98 213 661.3 0 213 99 115 661.3 0 115 100 157 661.3 0 157 101 116 661.3 0 116 102 36 661.3 0 36 103 25 661.3 0 25 104 20 661.3 0 20 105 32 661.3 0 32 106 35 661.3 0 35 107 77 661.3 0 77 108 97 661.3 0 97 109 98 661.3 0 98 110 97 661.3 0 97 111 97 661.3 0 97 112 96 661.3 0 96 113 448 661.3 0 448 114 541 661.3 0 541 115 1410 661.3 0 1410 116 326 661.3 0 326 117 140 661.3 0 140 118 128 661.3 0 128 119 101 661.3 0 101 120 149 661.3 0 149 121 100 661.3 0 100 122 167 661.3 0 167 123 150 661.3 0 150 124 213 661.3 0 213 125 104 661.3 0 104 126 113 661.3 0 113 127 118 661.3 0 118 128 309 661.3 0 309 129 316 661.3 0 316 130 391 661.3 0 391 131 197 661.3 0 197 132 143 661.3 0 143 133 136 661.3 0 136 134 336 661.3 0 336 135 363 661.3 0 363 136 306 661.3 0 306 137 253 661.3 0 253 138 369 661.3 0 369 139 536 661.3 0 536 140 199 661.3 0 199 141 117 661.3 0 117 142 158 661.3 0 158 143 169 661.3 0 169 144 167 661.3 0 167 145 120 661.3 0 120 146 129 661.3 0 129 147 110 661.3 0 110 148 134 661.3 0 134 149 137 661.3 0 137 150 135 661.3 0 135 151 109 661.3 0 109 152 128 661.3 0 128 153 169 661.3 0 169 154 118 661.3 0 118 155 171 661.3 0 171 156 120 661.3 0 120 157 140 661.3 0 140 158 109 661.3 0 109 159 77 661.3 0 77 160 68 661.3 0 68 161 80 661.3 0 80 162 125 661.3 0 125 163 166 661.3 0 166 164 191 661.3 0 191 165 143 661.3 0 143 166 101 661.3 0 101 167 149 661.3 0 149 168 174 661.3 0 174 169 179 661.3 0 179 170 154 661.3 0 154 171 112 661.3 0 112 172 101 661.3 0 101 173 133 661.3 0 133 174 94 661.3 0 94 175 101 661.3 0 101 176 91 661.3 0 91 177 136 661.3 0 136 178 143 661.3 0 143 179 128 661.3 0 128 180 124 661.3 0 124 181 129 661.3 0 129 182 113 661.3 0 113 183 133 661.3 0 133 184 161 661.3 0 161 185 150 661.3 0 150 186 109 661.3 0 109 187 95 661.3 0 95 188 153 661.3 0 153 189 147 661.3 0 147 190 192 661.3 0 192 191 198 661.3 0 198 192 135 661.3 0 135 193 93 661.3 0 93 194 71 661.3 0 71 195 138 661.3 0 138 196 116 661.3 0 116 197 175 661.3 0 175 198 102 661.3 0 102 199 64 661.3 0 64 200 153 661.3 0 153 201 114 661.3 0 114 202 179 661.3 0 179 203 343 661.3 0 343 204 236 661.3 0 236 205 179 661.3 0 179 206 183 661.3 0 183 207 170 661.3 0 170 208 143 661.3 0 143 209 191 661.3 0 191 210 246 661.3 0 246 211 195 661.3 0 195 212 162 661.3 0 162 213 114 661.3 0 114 214 131 661.3 0 131 215 163 661.3 0 163 216 202 661.3 0 202 217 144 661.3 0 144 218 104 661.3 0 104 219 94 661.3 0 94 220 106 661.3 0 106 221 135 661.3 0 135 222 100 661.3 0 100 223 158 661.3 0 158 224 239 661.3 0 239 225 524 661.3 0 524 226 503 661.3 0 503 227 667 661.3 0 667 228 278 661.3 0 278 229 87 661.3 0 87 230 91 661.3 0 91 231 142 661.3 0 142 232 150 661.3 0 150 233 143 661.3 0 143 234 192 661.3 0 192 235 233 661.3 0 233 236 345 661.3 0 345 237 370 661.3 0 370 238 102 661.3 0 102 239 94 661.3 0 94 240 84 661.3 0 84 241 104 661.3 0 104 242 137 661.3 0 137 243 163 661.3 0 163 244 132 661.3 0 132 245 97 661.3 0 97 246 59 661.3 0 59 247 55 661.3 0 55 248 51 661.3 0 51 249 86 661.3 0 86 250 132 661.3 0 132 251 120 661.3 0 120 252 263 661.3 0 263 253 185 661.3 0 185 254 110 661.3 0 110 255 83 661.3 0 83 256 65 661.3 0 65 257 46 661.3 0 46 258 77 661.3 0 77 259 80 661.3 0 80 260 109 661.3 0 109 261 158 661.3 0 158 262 184 661.3 0 184 263 259 661.3 0 259 264 196 661.3 0 196 265 180 661.3 0 180 266 133 661.3 0 133 267 34 661.3 0 34 268 45 661.3 0 45 269 117 661.3 0 117 270 304 661.3 0 304 271 212 661.3 0 212 272 143 661.3 0 143 273 77 661.3 0 77 274 68 661.3 0 68 275 155 661.3 0 155 276 115 661.3 0 115 277 102 661.3 0 102 278 111 661.3 0 111 279 86 661.3 0 86 280 60 661.3 0 60 281 122 661.3 0 122 282 94 661.3 0 94 283 96 661.3 0 96 284 120 661.3 0 120 285 76 661.3 0 76 286 63 661.3 0 63 287 59 661.3 0 59 288 65 661.3 0 65 289 177 661.3 0 177 290 276 661.3 0 276 291 233 661.3 0 233 292 108 661.3 0 108 293 131 661.3 0 131 294 100 661.3 0 100 295 179 661.3 0 179 296 310 661.3 0 310 297 355 661.3 0 355 298 218 661.3 0 218 299 176 661.3 0 176 300 162 661.3 0 162 301 123 661.3 0 123 Finished in 2.22 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 53,015 Reads with adapters: 19,098 (36.0%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 19,078 (36.0%) Total basepairs processed: 12,378,418 bp Total written (filtered): 5,260,589 bp (42.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19098 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 9 0.0 3 0 0 6 3 16 182 0.0 3 0 169 6 7 17 18719 0.0 3 18186 474 48 11 18 81 0.0 3 13 45 20 3 19 25 0.0 3 0 0 8 17 20 20 0.0 3 0 0 0 20 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 38 6 0.0 3 0 0 4 2 39 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 78 7 0.0 3 0 0 0 7 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.67 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 19,078 Reads with adapters: 548 (2.9%) Reads written (passing filters): 19,078 (100.0%) Total basepairs processed: 5,260,589 bp Total written (filtered): 5,258,937 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 548 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.6% C: 8.4% G: 33.0% T: 6.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 540 298.1 0 540 4 8 74.5 0 8