This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.66 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 534,236 (19.7%) Reads that were too short: 75,714 (2.8%) Reads written (passing filters): 458,522 (16.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 79,243,911 bp (9.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 534236 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 206 661.3 0 206 7 9696 661.3 0 9696 8 143 661.3 0 143 9 305 661.3 0 305 10 163 661.3 0 163 11 70 661.3 0 70 12 105 661.3 0 105 13 77 661.3 0 77 14 77 661.3 0 77 15 67 661.3 0 67 16 66 661.3 0 66 17 65 661.3 0 65 18 59 661.3 0 59 19 74 661.3 0 74 20 54 661.3 0 54 21 73 661.3 0 73 22 76 661.3 0 76 23 66 661.3 0 66 24 83 661.3 0 83 25 70 661.3 0 70 26 102 661.3 0 102 27 164 661.3 0 164 28 82 661.3 0 82 29 142 661.3 0 142 30 445 661.3 0 445 31 57 661.3 0 57 32 72 661.3 0 72 33 54 661.3 0 54 34 80 661.3 0 80 35 78 661.3 0 78 36 70 661.3 0 70 37 110 661.3 0 110 38 51 661.3 0 51 39 58 661.3 0 58 40 87 661.3 0 87 41 50 661.3 0 50 42 82 661.3 0 82 43 86 661.3 0 86 44 64 661.3 0 64 45 58 661.3 0 58 46 48 661.3 0 48 47 56 661.3 0 56 48 66 661.3 0 66 49 57 661.3 0 57 50 62 661.3 0 62 51 66 661.3 0 66 52 74 661.3 0 74 53 98 661.3 0 98 54 84 661.3 0 84 55 73 661.3 0 73 56 94 661.3 0 94 57 81 661.3 0 81 58 59 661.3 0 59 59 61 661.3 0 61 60 62 661.3 0 62 61 82 661.3 0 82 62 52 661.3 0 52 63 84 661.3 0 84 64 112 661.3 0 112 65 85 661.3 0 85 66 97 661.3 0 97 67 119 661.3 0 119 68 184 661.3 0 184 69 236 661.3 0 236 70 247 661.3 0 247 71 613 661.3 0 613 72 606 661.3 0 606 73 413 661.3 0 413 74 936 661.3 0 936 75 2055 661.3 0 2055 76 1521 661.3 0 1521 77 1248 661.3 0 1248 78 1770 661.3 0 1770 79 3414 661.3 0 3414 80 1872 661.3 0 1872 81 3259 661.3 0 3259 82 3229 661.3 0 3229 83 3240 661.3 0 3240 84 3007 661.3 0 3007 85 5175 661.3 0 5175 86 4300 661.3 0 4300 87 2251 661.3 0 2251 88 1730 661.3 0 1730 89 1977 661.3 0 1977 90 2123 661.3 0 2123 91 781 661.3 0 781 92 2437 661.3 0 2437 93 2174 661.3 0 2174 94 1201 661.3 0 1201 95 2287 661.3 0 2287 96 1620 661.3 0 1620 97 862 661.3 0 862 98 2147 661.3 0 2147 99 1357 661.3 0 1357 100 667 661.3 0 667 101 1542 661.3 0 1542 102 1234 661.3 0 1234 103 790 661.3 0 790 104 811 661.3 0 811 105 1437 661.3 0 1437 106 792 661.3 0 792 107 835 661.3 0 835 108 1315 661.3 0 1315 109 1002 661.3 0 1002 110 1269 661.3 0 1269 111 1559 661.3 0 1559 112 1518 661.3 0 1518 113 1932 661.3 0 1932 114 1961 661.3 0 1961 115 2023 661.3 0 2023 116 2297 661.3 0 2297 117 2813 661.3 0 2813 118 14714 661.3 0 14714 119 28438 661.3 0 28438 120 59173 661.3 0 59173 121 2806 661.3 0 2806 122 2726 661.3 0 2726 123 4374 661.3 0 4374 124 1453 661.3 0 1453 125 1037 661.3 0 1037 126 1545 661.3 0 1545 127 1383 661.3 0 1383 128 1252 661.3 0 1252 129 1566 661.3 0 1566 130 1460 661.3 0 1460 131 1526 661.3 0 1526 132 2226 661.3 0 2226 133 1638 661.3 0 1638 134 1796 661.3 0 1796 135 2007 661.3 0 2007 136 1774 661.3 0 1774 137 1926 661.3 0 1926 138 1995 661.3 0 1995 139 3368 661.3 0 3368 140 18653 661.3 0 18653 141 9865 661.3 0 9865 142 11389 661.3 0 11389 143 2745 661.3 0 2745 144 2937 661.3 0 2937 145 14262 661.3 0 14262 146 22767 661.3 0 22767 147 49024 661.3 0 49024 148 6564 661.3 0 6564 149 6812 661.3 0 6812 150 2188 661.3 0 2188 151 2219 661.3 0 2219 152 2003 661.3 0 2003 153 2330 661.3 0 2330 154 1248 661.3 0 1248 155 1335 661.3 0 1335 156 1128 661.3 0 1128 157 1118 661.3 0 1118 158 1478 661.3 0 1478 159 1379 661.3 0 1379 160 1237 661.3 0 1237 161 1032 661.3 0 1032 162 978 661.3 0 978 163 959 661.3 0 959 164 1411 661.3 0 1411 165 3207 661.3 0 3207 166 5060 661.3 0 5060 167 7544 661.3 0 7544 168 1262 661.3 0 1262 169 1087 661.3 0 1087 170 968 661.3 0 968 171 953 661.3 0 953 172 1083 661.3 0 1083 173 1131 661.3 0 1131 174 915 661.3 0 915 175 1111 661.3 0 1111 176 1058 661.3 0 1058 177 758 661.3 0 758 178 997 661.3 0 997 179 747 661.3 0 747 180 756 661.3 0 756 181 881 661.3 0 881 182 804 661.3 0 804 183 863 661.3 0 863 184 893 661.3 0 893 185 1028 661.3 0 1028 186 955 661.3 0 955 187 877 661.3 0 877 188 1022 661.3 0 1022 189 942 661.3 0 942 190 905 661.3 0 905 191 978 661.3 0 978 192 1139 661.3 0 1139 193 1428 661.3 0 1428 194 985 661.3 0 985 195 1279 661.3 0 1279 196 1283 661.3 0 1283 197 760 661.3 0 760 198 1139 661.3 0 1139 199 860 661.3 0 860 200 1119 661.3 0 1119 201 1173 661.3 0 1173 202 716 661.3 0 716 203 787 661.3 0 787 204 824 661.3 0 824 205 777 661.3 0 777 206 899 661.3 0 899 207 666 661.3 0 666 208 743 661.3 0 743 209 899 661.3 0 899 210 735 661.3 0 735 211 739 661.3 0 739 212 678 661.3 0 678 213 710 661.3 0 710 214 655 661.3 0 655 215 669 661.3 0 669 216 988 661.3 0 988 217 1023 661.3 0 1023 218 1052 661.3 0 1052 219 1034 661.3 0 1034 220 691 661.3 0 691 221 766 661.3 0 766 222 590 661.3 0 590 223 569 661.3 0 569 224 575 661.3 0 575 225 494 661.3 0 494 226 592 661.3 0 592 227 619 661.3 0 619 228 606 661.3 0 606 229 641 661.3 0 641 230 665 661.3 0 665 231 701 661.3 0 701 232 639 661.3 0 639 233 603 661.3 0 603 234 610 661.3 0 610 235 648 661.3 0 648 236 623 661.3 0 623 237 857 661.3 0 857 238 835 661.3 0 835 239 819 661.3 0 819 240 999 661.3 0 999 241 748 661.3 0 748 242 576 661.3 0 576 243 487 661.3 0 487 244 586 661.3 0 586 245 522 661.3 0 522 246 566 661.3 0 566 247 1219 661.3 0 1219 248 1151 661.3 0 1151 249 763 661.3 0 763 250 814 661.3 0 814 251 650 661.3 0 650 252 452 661.3 0 452 253 423 661.3 0 423 254 403 661.3 0 403 255 412 661.3 0 412 256 409 661.3 0 409 257 529 661.3 0 529 258 524 661.3 0 524 259 630 661.3 0 630 260 846 661.3 0 846 261 1429 661.3 0 1429 262 721 661.3 0 721 263 737 661.3 0 737 264 1003 661.3 0 1003 265 471 661.3 0 471 266 525 661.3 0 525 267 578 661.3 0 578 268 554 661.3 0 554 269 569 661.3 0 569 270 743 661.3 0 743 271 660 661.3 0 660 272 587 661.3 0 587 273 846 661.3 0 846 274 745 661.3 0 745 275 734 661.3 0 734 276 738 661.3 0 738 277 600 661.3 0 600 278 570 661.3 0 570 279 833 661.3 0 833 280 591 661.3 0 591 281 544 661.3 0 544 282 607 661.3 0 607 283 502 661.3 0 502 284 507 661.3 0 507 285 601 661.3 0 601 286 481 661.3 0 481 287 726 661.3 0 726 288 913 661.3 0 913 289 780 661.3 0 780 290 948 661.3 0 948 291 852 661.3 0 852 292 767 661.3 0 767 293 829 661.3 0 829 294 938 661.3 0 938 295 936 661.3 0 936 296 1086 661.3 0 1086 297 1575 661.3 0 1575 298 2340 661.3 0 2340 299 1346 661.3 0 1346 300 1015 661.3 0 1015 301 1741 661.3 0 1741 Finished in 14.19 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 458,522 Reads with adapters: 12,128 (2.6%) Reads that were too short: 604 (0.1%) Reads written (passing filters): 11,524 (2.5%) Total basepairs processed: 79,243,911 bp Total written (filtered): 2,907,092 bp (3.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12128 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 16 66 0.0 3 0 56 7 3 17 9595 0.0 3 9350 204 29 12 18 30 0.0 3 2 14 4 10 19 16 0.0 3 0 0 11 5 20 8 0.0 3 2 0 0 6 22 1 0.0 3 0 0 0 1 23 17 0.0 3 0 0 1 16 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 823 0.0 3 0 0 1 822 27 7 0.0 3 0 0 0 7 29 101 0.0 3 0 0 5 96 31 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 1 41 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 1 1 48 5 0.0 3 0 0 0 5 49 2 0.0 3 0 0 0 2 50 30 0.0 3 0 0 0 30 51 2 0.0 3 0 0 0 2 52 5 0.0 3 0 0 0 5 53 37 0.0 3 0 0 2 35 54 9 0.0 3 0 0 0 9 55 3 0.0 3 0 0 0 3 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 68 126 0.0 3 0 0 0 126 69 3 0.0 3 1 0 0 2 70 4 0.0 3 0 0 0 4 72 4 0.0 3 0 0 0 4 73 82 0.0 3 0 0 0 82 74 404 0.0 3 0 0 1 403 75 2 0.0 3 0 0 0 2 76 16 0.0 3 0 0 0 16 77 61 0.0 3 0 0 1 60 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 82 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 86 4 0.0 3 0 0 0 4 87 3 0.0 3 0 0 0 3 89 5 0.0 3 0 0 0 5 91 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 97 4 0.0 3 0 0 0 4 99 2 0.0 3 0 0 1 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 16 0.0 3 0 0 0 16 108 5 0.0 3 0 0 0 5 110 10 0.0 3 0 0 1 9 111 7 0.0 3 0 0 0 7 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 127 6 0.0 3 0 0 0 6 128 43 0.0 3 0 0 3 40 129 19 0.0 3 0 0 0 19 131 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 133 5 0.0 3 0 0 0 5 134 1 0.0 3 0 0 0 1 135 5 0.0 3 0 0 0 5 137 3 0.0 3 0 0 0 3 138 177 0.0 3 0 0 2 175 139 14 0.0 3 0 0 0 14 140 39 0.0 3 0 0 1 38 141 110 0.0 3 0 0 2 108 142 13 0.0 3 0 0 0 13 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 6 0.0 3 0 0 0 6 147 19 0.0 3 0 0 1 18 148 16 0.0 3 0 0 0 16 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 7 0.0 3 0 0 2 5 152 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 156 8 0.0 3 0 0 1 7 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 175 4 0.0 3 0 0 1 3 176 4 0.0 3 0 0 0 4 180 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 11,524 Reads with adapters: 939 (8.1%) Reads written (passing filters): 11,524 (100.0%) Total basepairs processed: 2,907,092 bp Total written (filtered): 2,904,259 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 939 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.0% C: 32.7% G: 38.0% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 929 180.1 0 929 4 5 45.0 0 5 5 4 11.3 0 4 6 1 2.8 0 1