This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.60 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 128,386 (4.7%) Reads that were too short: 53,946 (2.0%) Reads written (passing filters): 74,440 (2.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 14,056,424 bp (1.7%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 128386 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 247 661.3 0 247 7 8109 661.3 0 8109 8 8260 661.3 0 8260 9 20 661.3 0 20 10 19 661.3 0 19 11 21 661.3 0 21 12 14 661.3 0 14 13 15 661.3 0 15 14 23 661.3 0 23 15 22 661.3 0 22 16 26 661.3 0 26 17 24 661.3 0 24 18 28 661.3 0 28 19 32 661.3 0 32 20 14 661.3 0 14 21 11 661.3 0 11 22 23 661.3 0 23 23 29 661.3 0 29 24 28 661.3 0 28 25 20 661.3 0 20 26 15 661.3 0 15 27 16 661.3 0 16 28 14 661.3 0 14 29 16 661.3 0 16 30 16 661.3 0 16 31 34 661.3 0 34 32 20 661.3 0 20 33 17 661.3 0 17 34 23 661.3 0 23 35 11 661.3 0 11 36 24 661.3 0 24 37 47 661.3 0 47 38 13 661.3 0 13 39 17 661.3 0 17 40 21 661.3 0 21 41 20 661.3 0 20 42 145 661.3 0 145 43 8 661.3 0 8 44 8 661.3 0 8 45 13 661.3 0 13 46 25 661.3 0 25 47 21 661.3 0 21 48 17 661.3 0 17 49 7 661.3 0 7 50 9 661.3 0 9 51 21 661.3 0 21 52 15 661.3 0 15 53 22 661.3 0 22 54 15 661.3 0 15 55 19 661.3 0 19 56 18 661.3 0 18 57 24 661.3 0 24 58 27 661.3 0 27 59 16 661.3 0 16 60 18 661.3 0 18 61 13 661.3 0 13 62 23 661.3 0 23 63 24 661.3 0 24 64 19 661.3 0 19 65 19 661.3 0 19 66 26 661.3 0 26 67 28 661.3 0 28 68 14 661.3 0 14 69 28 661.3 0 28 70 17 661.3 0 17 71 12 661.3 0 12 72 16 661.3 0 16 73 16 661.3 0 16 74 20 661.3 0 20 75 20 661.3 0 20 76 14 661.3 0 14 77 17 661.3 0 17 78 16 661.3 0 16 79 16 661.3 0 16 80 22 661.3 0 22 81 57 661.3 0 57 82 85 661.3 0 85 83 110 661.3 0 110 84 179 661.3 0 179 85 217 661.3 0 217 86 182 661.3 0 182 87 188 661.3 0 188 88 280 661.3 0 280 89 100 661.3 0 100 90 73 661.3 0 73 91 153 661.3 0 153 92 97 661.3 0 97 93 145 661.3 0 145 94 219 661.3 0 219 95 320 661.3 0 320 96 977 661.3 0 977 97 543 661.3 0 543 98 459 661.3 0 459 99 491 661.3 0 491 100 192 661.3 0 192 101 306 661.3 0 306 102 460 661.3 0 460 103 435 661.3 0 435 104 491 661.3 0 491 105 537 661.3 0 537 106 620 661.3 0 620 107 562 661.3 0 562 108 654 661.3 0 654 109 691 661.3 0 691 110 853 661.3 0 853 111 407 661.3 0 407 112 664 661.3 0 664 113 602 661.3 0 602 114 1027 661.3 0 1027 115 1253 661.3 0 1253 116 624 661.3 0 624 117 372 661.3 0 372 118 208 661.3 0 208 119 162 661.3 0 162 120 149 661.3 0 149 121 312 661.3 0 312 122 539 661.3 0 539 123 486 661.3 0 486 124 361 661.3 0 361 125 227 661.3 0 227 126 361 661.3 0 361 127 389 661.3 0 389 128 387 661.3 0 387 129 640 661.3 0 640 130 1007 661.3 0 1007 131 531 661.3 0 531 132 485 661.3 0 485 133 425 661.3 0 425 134 311 661.3 0 311 135 501 661.3 0 501 136 541 661.3 0 541 137 945 661.3 0 945 138 569 661.3 0 569 139 391 661.3 0 391 140 326 661.3 0 326 141 460 661.3 0 460 142 552 661.3 0 552 143 432 661.3 0 432 144 393 661.3 0 393 145 328 661.3 0 328 146 419 661.3 0 419 147 425 661.3 0 425 148 747 661.3 0 747 149 575 661.3 0 575 150 518 661.3 0 518 151 510 661.3 0 510 152 383 661.3 0 383 153 437 661.3 0 437 154 595 661.3 0 595 155 668 661.3 0 668 156 840 661.3 0 840 157 876 661.3 0 876 158 712 661.3 0 712 159 611 661.3 0 611 160 347 661.3 0 347 161 443 661.3 0 443 162 395 661.3 0 395 163 823 661.3 0 823 164 653 661.3 0 653 165 544 661.3 0 544 166 539 661.3 0 539 167 540 661.3 0 540 168 492 661.3 0 492 169 383 661.3 0 383 170 380 661.3 0 380 171 326 661.3 0 326 172 332 661.3 0 332 173 287 661.3 0 287 174 363 661.3 0 363 175 522 661.3 0 522 176 630 661.3 0 630 177 455 661.3 0 455 178 410 661.3 0 410 179 429 661.3 0 429 180 350 661.3 0 350 181 477 661.3 0 477 182 507 661.3 0 507 183 531 661.3 0 531 184 440 661.3 0 440 185 417 661.3 0 417 186 464 661.3 0 464 187 522 661.3 0 522 188 415 661.3 0 415 189 366 661.3 0 366 190 334 661.3 0 334 191 343 661.3 0 343 192 551 661.3 0 551 193 627 661.3 0 627 194 617 661.3 0 617 195 415 661.3 0 415 196 614 661.3 0 614 197 301 661.3 0 301 198 398 661.3 0 398 199 511 661.3 0 511 200 644 661.3 0 644 201 807 661.3 0 807 202 615 661.3 0 615 203 387 661.3 0 387 204 507 661.3 0 507 205 496 661.3 0 496 206 470 661.3 0 470 207 504 661.3 0 504 208 556 661.3 0 556 209 927 661.3 0 927 210 777 661.3 0 777 211 546 661.3 0 546 212 350 661.3 0 350 213 334 661.3 0 334 214 364 661.3 0 364 215 356 661.3 0 356 216 396 661.3 0 396 217 494 661.3 0 494 218 496 661.3 0 496 219 505 661.3 0 505 220 398 661.3 0 398 221 519 661.3 0 519 222 722 661.3 0 722 223 514 661.3 0 514 224 460 661.3 0 460 225 419 661.3 0 419 226 404 661.3 0 404 227 428 661.3 0 428 228 485 661.3 0 485 229 487 661.3 0 487 230 506 661.3 0 506 231 433 661.3 0 433 232 383 661.3 0 383 233 376 661.3 0 376 234 379 661.3 0 379 235 471 661.3 0 471 236 640 661.3 0 640 237 589 661.3 0 589 238 629 661.3 0 629 239 480 661.3 0 480 240 590 661.3 0 590 241 583 661.3 0 583 242 631 661.3 0 631 243 591 661.3 0 591 244 875 661.3 0 875 245 931 661.3 0 931 246 1000 661.3 0 1000 247 827 661.3 0 827 248 878 661.3 0 878 249 621 661.3 0 621 250 265 661.3 0 265 251 246 661.3 0 246 252 163 661.3 0 163 253 419 661.3 0 419 254 685 661.3 0 685 255 719 661.3 0 719 256 568 661.3 0 568 257 683 661.3 0 683 258 772 661.3 0 772 259 649 661.3 0 649 260 492 661.3 0 492 261 371 661.3 0 371 262 362 661.3 0 362 263 523 661.3 0 523 264 525 661.3 0 525 265 507 661.3 0 507 266 477 661.3 0 477 267 637 661.3 0 637 268 611 661.3 0 611 269 781 661.3 0 781 270 588 661.3 0 588 271 459 661.3 0 459 272 441 661.3 0 441 273 476 661.3 0 476 274 604 661.3 0 604 275 439 661.3 0 439 276 479 661.3 0 479 277 398 661.3 0 398 278 351 661.3 0 351 279 512 661.3 0 512 280 653 661.3 0 653 281 485 661.3 0 485 282 647 661.3 0 647 283 376 661.3 0 376 284 417 661.3 0 417 285 504 661.3 0 504 286 671 661.3 0 671 287 649 661.3 0 649 288 638 661.3 0 638 289 421 661.3 0 421 290 429 661.3 0 429 291 366 661.3 0 366 292 633 661.3 0 633 293 731 661.3 0 731 294 788 661.3 0 788 295 941 661.3 0 941 296 743 661.3 0 743 297 432 661.3 0 432 298 400 661.3 0 400 299 394 661.3 0 394 300 306 661.3 0 306 301 791 661.3 0 791 Finished in 2.93 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 74,440 Reads with adapters: 8,542 (11.5%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 8,516 (11.4%) Total basepairs processed: 14,056,424 bp Total written (filtered): 2,321,388 bp (16.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8542 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 3 19 83 0.0 3 0 79 2 0 2 20 8371 0.0 4 8282 80 6 2 1 21 23 0.0 4 4 19 22 2 0.0 4 0 0 1 1 23 2 0.0 4 0 0 0 2 24 1 0.0 4 0 0 0 0 1 26 8 0.0 4 0 1 0 0 7 29 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 73 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 127 16 0.0 4 0 0 0 0 16 137 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 8,516 Reads with adapters: 558 (6.6%) Reads written (passing filters): 8,516 (100.0%) Total basepairs processed: 2,321,388 bp Total written (filtered): 2,235,461 bp (96.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 558 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 56.3% C: 2.7% G: 40.7% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 5 1 8.3 0 1 10 19 0.0 1 3 16 11 211 0.0 1 1 210 151 2 0.0 1 0 2 203 10 0.0 1 9 1 209 1 0.0 1 1 215 2 0.0 1 1 1 216 1 0.0 1 1 220 1 0.0 1 1 224 6 0.0 1 6 225 2 0.0 1 2 231 1 0.0 1 1 258 2 0.0 1 2 259 284 0.0 1 268 16 260 10 0.0 1 10 261 3 0.0 1 2 1 262 2 0.0 1 1 1