This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.03 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 50,935 (1.9%) Reads that were too short: 7,443 (0.3%) Reads written (passing filters): 43,492 (1.6%) Total basepairs processed: 815,312,680 bp Total written (filtered): 6,749,927 bp (0.8%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 50935 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2205 661.3 0 2205 7 34 661.3 0 34 8 32 661.3 0 32 9 71 661.3 0 71 10 22 661.3 0 22 11 29 661.3 0 29 12 29 661.3 0 29 13 19 661.3 0 19 14 44 661.3 0 44 15 29 661.3 0 29 16 19 661.3 0 19 17 32 661.3 0 32 18 30 661.3 0 30 19 46 661.3 0 46 20 30 661.3 0 30 21 35 661.3 0 35 22 33 661.3 0 33 23 36 661.3 0 36 24 35 661.3 0 35 25 23 661.3 0 23 26 26 661.3 0 26 27 37 661.3 0 37 28 56 661.3 0 56 29 28 661.3 0 28 30 32 661.3 0 32 31 40 661.3 0 40 32 47 661.3 0 47 33 322 661.3 0 322 34 108 661.3 0 108 35 235 661.3 0 235 36 44 661.3 0 44 37 76 661.3 0 76 38 21 661.3 0 21 39 27 661.3 0 27 40 19 661.3 0 19 41 17 661.3 0 17 42 38 661.3 0 38 43 33 661.3 0 33 44 30 661.3 0 30 45 71 661.3 0 71 46 31 661.3 0 31 47 44 661.3 0 44 48 22 661.3 0 22 49 43 661.3 0 43 50 45 661.3 0 45 51 29 661.3 0 29 52 49 661.3 0 49 53 37 661.3 0 37 54 22 661.3 0 22 55 39 661.3 0 39 56 51 661.3 0 51 57 165 661.3 0 165 58 43 661.3 0 43 59 79 661.3 0 79 60 30 661.3 0 30 61 24 661.3 0 24 62 36 661.3 0 36 63 25 661.3 0 25 64 39 661.3 0 39 65 99 661.3 0 99 66 26 661.3 0 26 67 18 661.3 0 18 68 34 661.3 0 34 69 26 661.3 0 26 70 25 661.3 0 25 71 28 661.3 0 28 72 104 661.3 0 104 73 32 661.3 0 32 74 48 661.3 0 48 75 26 661.3 0 26 76 28 661.3 0 28 77 35 661.3 0 35 78 23 661.3 0 23 79 33 661.3 0 33 80 29 661.3 0 29 81 25 661.3 0 25 82 20 661.3 0 20 83 29 661.3 0 29 84 61 661.3 0 61 85 58 661.3 0 58 86 68 661.3 0 68 87 55 661.3 0 55 88 54 661.3 0 54 89 35 661.3 0 35 90 54 661.3 0 54 91 35 661.3 0 35 92 53 661.3 0 53 93 79 661.3 0 79 94 109 661.3 0 109 95 50 661.3 0 50 96 65 661.3 0 65 97 71 661.3 0 71 98 46 661.3 0 46 99 62 661.3 0 62 100 54 661.3 0 54 101 53 661.3 0 53 102 54 661.3 0 54 103 49 661.3 0 49 104 47 661.3 0 47 105 88 661.3 0 88 106 615 661.3 0 615 107 1415 661.3 0 1415 108 1395 661.3 0 1395 109 1719 661.3 0 1719 110 41 661.3 0 41 111 26 661.3 0 26 112 27 661.3 0 27 113 34 661.3 0 34 114 44 661.3 0 44 115 36 661.3 0 36 116 32 661.3 0 32 117 37 661.3 0 37 118 34 661.3 0 34 119 38 661.3 0 38 120 37 661.3 0 37 121 70 661.3 0 70 122 63 661.3 0 63 123 66 661.3 0 66 124 47 661.3 0 47 125 42 661.3 0 42 126 51 661.3 0 51 127 269 661.3 0 269 128 531 661.3 0 531 129 1081 661.3 0 1081 130 77 661.3 0 77 131 141 661.3 0 141 132 64 661.3 0 64 133 26 661.3 0 26 134 68 661.3 0 68 135 58 661.3 0 58 136 143 661.3 0 143 137 117 661.3 0 117 138 92 661.3 0 92 139 33 661.3 0 33 140 67 661.3 0 67 141 109 661.3 0 109 142 69 661.3 0 69 143 219 661.3 0 219 144 312 661.3 0 312 145 554 661.3 0 554 146 60 661.3 0 60 147 99 661.3 0 99 148 63 661.3 0 63 149 83 661.3 0 83 150 60 661.3 0 60 151 47 661.3 0 47 152 82 661.3 0 82 153 163 661.3 0 163 154 337 661.3 0 337 155 625 661.3 0 625 156 179 661.3 0 179 157 59 661.3 0 59 158 48 661.3 0 48 159 63 661.3 0 63 160 55 661.3 0 55 161 67 661.3 0 67 162 48 661.3 0 48 163 150 661.3 0 150 164 36 661.3 0 36 165 63 661.3 0 63 166 103 661.3 0 103 167 73 661.3 0 73 168 81 661.3 0 81 169 178 661.3 0 178 170 77 661.3 0 77 171 93 661.3 0 93 172 45 661.3 0 45 173 68 661.3 0 68 174 140 661.3 0 140 175 129 661.3 0 129 176 101 661.3 0 101 177 61 661.3 0 61 178 50 661.3 0 50 179 74 661.3 0 74 180 89 661.3 0 89 181 123 661.3 0 123 182 186 661.3 0 186 183 276 661.3 0 276 184 425 661.3 0 425 185 793 661.3 0 793 186 14476 661.3 0 14476 187 2243 661.3 0 2243 188 3807 661.3 0 3807 189 49 661.3 0 49 190 38 661.3 0 38 191 48 661.3 0 48 192 71 661.3 0 71 193 34 661.3 0 34 194 38 661.3 0 38 195 33 661.3 0 33 196 31 661.3 0 31 197 30 661.3 0 30 198 34 661.3 0 34 199 36 661.3 0 36 200 46 661.3 0 46 201 46 661.3 0 46 202 34 661.3 0 34 203 62 661.3 0 62 204 125 661.3 0 125 205 122 661.3 0 122 206 62 661.3 0 62 207 37 661.3 0 37 208 72 661.3 0 72 209 62 661.3 0 62 210 59 661.3 0 59 211 56 661.3 0 56 212 64 661.3 0 64 213 61 661.3 0 61 214 62 661.3 0 62 215 65 661.3 0 65 216 109 661.3 0 109 217 1004 661.3 0 1004 218 315 661.3 0 315 219 472 661.3 0 472 220 30 661.3 0 30 221 47 661.3 0 47 222 58 661.3 0 58 223 37 661.3 0 37 224 34 661.3 0 34 225 36 661.3 0 36 226 73 661.3 0 73 227 71 661.3 0 71 228 46 661.3 0 46 229 50 661.3 0 50 230 38 661.3 0 38 231 33 661.3 0 33 232 55 661.3 0 55 233 46 661.3 0 46 234 67 661.3 0 67 235 53 661.3 0 53 236 51 661.3 0 51 237 44 661.3 0 44 238 34 661.3 0 34 239 44 661.3 0 44 240 26 661.3 0 26 241 47 661.3 0 47 242 37 661.3 0 37 243 79 661.3 0 79 244 70 661.3 0 70 245 79 661.3 0 79 246 48 661.3 0 48 247 40 661.3 0 40 248 29 661.3 0 29 249 29 661.3 0 29 250 44 661.3 0 44 251 62 661.3 0 62 252 187 661.3 0 187 253 118 661.3 0 118 254 100 661.3 0 100 255 94 661.3 0 94 256 59 661.3 0 59 257 45 661.3 0 45 258 53 661.3 0 53 259 25 661.3 0 25 260 28 661.3 0 28 261 36 661.3 0 36 262 87 661.3 0 87 263 62 661.3 0 62 264 44 661.3 0 44 265 35 661.3 0 35 266 55 661.3 0 55 267 55 661.3 0 55 268 69 661.3 0 69 269 32 661.3 0 32 270 31 661.3 0 31 271 40 661.3 0 40 272 69 661.3 0 69 273 52 661.3 0 52 274 17 661.3 0 17 275 37 661.3 0 37 276 35 661.3 0 35 277 51 661.3 0 51 278 149 661.3 0 149 279 83 661.3 0 83 280 115 661.3 0 115 281 72 661.3 0 72 282 62 661.3 0 62 283 38 661.3 0 38 284 29 661.3 0 29 285 52 661.3 0 52 286 59 661.3 0 59 287 49 661.3 0 49 288 29 661.3 0 29 289 23 661.3 0 23 290 22 661.3 0 22 291 41 661.3 0 41 292 35 661.3 0 35 293 45 661.3 0 45 294 51 661.3 0 51 295 33 661.3 0 33 296 92 661.3 0 92 297 143 661.3 0 143 298 99 661.3 0 99 299 131 661.3 0 131 300 76 661.3 0 76 301 19 661.3 0 19 Finished in 1.39 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 43,492 Reads with adapters: 2,282 (5.2%) Reads that were too short: 35 (0.1%) Reads written (passing filters): 2,247 (5.2%) Total basepairs processed: 6,749,927 bp Total written (filtered): 607,820 bp (9.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2282 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 1 17 3 0.0 3 3 18 5 0.0 3 1 0 4 19 37 0.0 3 5 28 0 4 20 2119 0.0 3 2076 27 8 8 21 12 0.0 3 0 9 1 2 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 31 5 0.0 3 0 0 0 5 33 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 22 0.0 3 0 0 0 22 61 6 0.0 3 0 0 0 6 62 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 7 0.0 3 0 0 0 7 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 132 8 0.0 3 0 0 0 8 134 4 0.0 3 0 0 0 4 137 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 156 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 1 260 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 2,247 Reads with adapters: 147 (6.5%) Reads written (passing filters): 2,247 (100.0%) Total basepairs processed: 607,820 bp Total written (filtered): 607,378 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 147 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.3% C: 1.4% G: 78.9% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 146 35.1 0 146 4 1 8.8 0 1