This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.28 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 30,898 (1.1%) Reads that were too short: 6,048 (0.2%) Reads written (passing filters): 24,850 (0.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 5,735,162 bp (0.7%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 30898 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5720 661.3 0 5720 7 115 661.3 0 115 8 4608 661.3 0 4608 9 43 661.3 0 43 10 36 661.3 0 36 11 21 661.3 0 21 12 29 661.3 0 29 13 30 661.3 0 30 14 31 661.3 0 31 15 43 661.3 0 43 16 39 661.3 0 39 17 24 661.3 0 24 18 22 661.3 0 22 19 25 661.3 0 25 20 30 661.3 0 30 21 32 661.3 0 32 22 30 661.3 0 30 23 37 661.3 0 37 24 18 661.3 0 18 25 39 661.3 0 39 26 29 661.3 0 29 27 36 661.3 0 36 28 24 661.3 0 24 29 15 661.3 0 15 30 29 661.3 0 29 31 20 661.3 0 20 32 35 661.3 0 35 33 21 661.3 0 21 34 30 661.3 0 30 35 30 661.3 0 30 36 29 661.3 0 29 37 28 661.3 0 28 38 23 661.3 0 23 39 29 661.3 0 29 40 32 661.3 0 32 41 27 661.3 0 27 42 50 661.3 0 50 43 28 661.3 0 28 44 19 661.3 0 19 45 39 661.3 0 39 46 34 661.3 0 34 47 29 661.3 0 29 48 32 661.3 0 32 49 507 661.3 0 507 50 105 661.3 0 105 51 31 661.3 0 31 52 33 661.3 0 33 53 32 661.3 0 32 54 23 661.3 0 23 55 24 661.3 0 24 56 20 661.3 0 20 57 24 661.3 0 24 58 32 661.3 0 32 59 42 661.3 0 42 60 42 661.3 0 42 61 21 661.3 0 21 62 31 661.3 0 31 63 18 661.3 0 18 64 15 661.3 0 15 65 14 661.3 0 14 66 70 661.3 0 70 67 53 661.3 0 53 68 31 661.3 0 31 69 18 661.3 0 18 70 19 661.3 0 19 71 19 661.3 0 19 72 12 661.3 0 12 73 14 661.3 0 14 74 16 661.3 0 16 75 11 661.3 0 11 76 20 661.3 0 20 77 16 661.3 0 16 78 14 661.3 0 14 79 21 661.3 0 21 80 20 661.3 0 20 81 16 661.3 0 16 82 44 661.3 0 44 83 31 661.3 0 31 84 28 661.3 0 28 85 65 661.3 0 65 86 39 661.3 0 39 87 27 661.3 0 27 88 38 661.3 0 38 89 27 661.3 0 27 90 21 661.3 0 21 91 122 661.3 0 122 92 67 661.3 0 67 93 55 661.3 0 55 94 53 661.3 0 53 95 37 661.3 0 37 96 16 661.3 0 16 97 36 661.3 0 36 98 68 661.3 0 68 99 23 661.3 0 23 100 23 661.3 0 23 101 25 661.3 0 25 102 29 661.3 0 29 103 28 661.3 0 28 104 69 661.3 0 69 105 50 661.3 0 50 106 103 661.3 0 103 107 38 661.3 0 38 108 29 661.3 0 29 109 43 661.3 0 43 110 42 661.3 0 42 111 176 661.3 0 176 112 222 661.3 0 222 113 1586 661.3 0 1586 114 70 661.3 0 70 115 186 661.3 0 186 116 204 661.3 0 204 117 585 661.3 0 585 118 1281 661.3 0 1281 119 1366 661.3 0 1366 120 40 661.3 0 40 121 32 661.3 0 32 122 24 661.3 0 24 123 39 661.3 0 39 124 16 661.3 0 16 125 53 661.3 0 53 126 94 661.3 0 94 127 44 661.3 0 44 128 56 661.3 0 56 129 21 661.3 0 21 130 34 661.3 0 34 131 47 661.3 0 47 132 41 661.3 0 41 133 186 661.3 0 186 134 36 661.3 0 36 135 17 661.3 0 17 136 26 661.3 0 26 137 27 661.3 0 27 138 40 661.3 0 40 139 27 661.3 0 27 140 21 661.3 0 21 141 37 661.3 0 37 142 46 661.3 0 46 143 30 661.3 0 30 144 52 661.3 0 52 145 50 661.3 0 50 146 59 661.3 0 59 147 154 661.3 0 154 148 58 661.3 0 58 149 50 661.3 0 50 150 55 661.3 0 55 151 136 661.3 0 136 152 252 661.3 0 252 153 447 661.3 0 447 154 183 661.3 0 183 155 54 661.3 0 54 156 53 661.3 0 53 157 45 661.3 0 45 158 23 661.3 0 23 159 44 661.3 0 44 160 34 661.3 0 34 161 36 661.3 0 36 162 64 661.3 0 64 163 58 661.3 0 58 164 55 661.3 0 55 165 38 661.3 0 38 166 53 661.3 0 53 167 34 661.3 0 34 168 30 661.3 0 30 169 55 661.3 0 55 170 34 661.3 0 34 171 52 661.3 0 52 172 56 661.3 0 56 173 46 661.3 0 46 174 26 661.3 0 26 175 36 661.3 0 36 176 30 661.3 0 30 177 49 661.3 0 49 178 49 661.3 0 49 179 90 661.3 0 90 180 136 661.3 0 136 181 173 661.3 0 173 182 82 661.3 0 82 183 139 661.3 0 139 184 50 661.3 0 50 185 67 661.3 0 67 186 28 661.3 0 28 187 30 661.3 0 30 188 32 661.3 0 32 189 40 661.3 0 40 190 46 661.3 0 46 191 36 661.3 0 36 192 43 661.3 0 43 193 29 661.3 0 29 194 22 661.3 0 22 195 34 661.3 0 34 196 35 661.3 0 35 197 41 661.3 0 41 198 16 661.3 0 16 199 37 661.3 0 37 200 46 661.3 0 46 201 38 661.3 0 38 202 31 661.3 0 31 203 20 661.3 0 20 204 35 661.3 0 35 205 48 661.3 0 48 206 31 661.3 0 31 207 40 661.3 0 40 208 37 661.3 0 37 209 34 661.3 0 34 210 32 661.3 0 32 211 51 661.3 0 51 212 43 661.3 0 43 213 62 661.3 0 62 214 59 661.3 0 59 215 43 661.3 0 43 216 67 661.3 0 67 217 92 661.3 0 92 218 283 661.3 0 283 219 120 661.3 0 120 220 101 661.3 0 101 221 81 661.3 0 81 222 33 661.3 0 33 223 53 661.3 0 53 224 36 661.3 0 36 225 66 661.3 0 66 226 81 661.3 0 81 227 53 661.3 0 53 228 61 661.3 0 61 229 64 661.3 0 64 230 59 661.3 0 59 231 98 661.3 0 98 232 122 661.3 0 122 233 272 661.3 0 272 234 76 661.3 0 76 235 89 661.3 0 89 236 94 661.3 0 94 237 119 661.3 0 119 238 46 661.3 0 46 239 58 661.3 0 58 240 55 661.3 0 55 241 32 661.3 0 32 242 30 661.3 0 30 243 26 661.3 0 26 244 55 661.3 0 55 245 79 661.3 0 79 246 104 661.3 0 104 247 64 661.3 0 64 248 85 661.3 0 85 249 95 661.3 0 95 250 87 661.3 0 87 251 32 661.3 0 32 252 48 661.3 0 48 253 41 661.3 0 41 254 96 661.3 0 96 255 109 661.3 0 109 256 100 661.3 0 100 257 44 661.3 0 44 258 44 661.3 0 44 259 58 661.3 0 58 260 36 661.3 0 36 261 28 661.3 0 28 262 20 661.3 0 20 263 26 661.3 0 26 264 43 661.3 0 43 265 57 661.3 0 57 266 52 661.3 0 52 267 38 661.3 0 38 268 68 661.3 0 68 269 51 661.3 0 51 270 59 661.3 0 59 271 30 661.3 0 30 272 55 661.3 0 55 273 66 661.3 0 66 274 32 661.3 0 32 275 47 661.3 0 47 276 40 661.3 0 40 277 37 661.3 0 37 278 36 661.3 0 36 279 34 661.3 0 34 280 82 661.3 0 82 281 78 661.3 0 78 282 54 661.3 0 54 283 45 661.3 0 45 284 100 661.3 0 100 285 29 661.3 0 29 286 51 661.3 0 51 287 68 661.3 0 68 288 100 661.3 0 100 289 67 661.3 0 67 290 31 661.3 0 31 291 33 661.3 0 33 292 44 661.3 0 44 293 58 661.3 0 58 294 56 661.3 0 56 295 42 661.3 0 42 296 41 661.3 0 41 297 40 661.3 0 40 298 28 661.3 0 28 299 22 661.3 0 22 300 23 661.3 0 23 301 27 661.3 0 27 Finished in 1.02 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 24,850 Reads with adapters: 4,798 (19.3%) Reads that were too short: 44 (0.2%) Reads written (passing filters): 4,754 (19.1%) Total basepairs processed: 5,735,162 bp Total written (filtered): 1,299,255 bp (22.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4798 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 5 0.0 3 0 2 2 1 17 111 0.0 3 57 44 7 3 18 4547 0.0 3 4419 102 21 5 19 21 0.0 3 1 7 3 10 20 8 0.0 3 1 0 2 5 21 5 0.0 3 0 0 0 5 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 11 0.0 3 0 0 0 11 42 1 0.0 3 1 43 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 54 9 0.0 3 0 0 0 9 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 61 3 0.0 3 0 0 0 3 67 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 102 1 0.0 3 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 166 9 0.0 3 0 0 0 9 167 1 0.0 3 0 0 0 1 169 24 0.0 3 0 0 0 24 170 3 0.0 3 0 0 0 3 193 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 284 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 4,754 Reads with adapters: 238 (5.0%) Reads written (passing filters): 4,754 (100.0%) Total basepairs processed: 1,299,255 bp Total written (filtered): 1,298,536 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 238 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.8% C: 23.5% G: 22.3% T: 8.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 233 74.3 0 233 4 5 18.6 0 5