This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.54 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 43,024 (1.6%) Reads that were too short: 22,330 (0.8%) Reads written (passing filters): 20,694 (0.8%) Total basepairs processed: 815,312,680 bp Total written (filtered): 4,494,777 bp (0.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 43024 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 218 661.3 0 218 7 8489 661.3 0 8489 8 27 661.3 0 27 9 10 661.3 0 10 10 7 661.3 0 7 11 13 661.3 0 13 12 19 661.3 0 19 13 13 661.3 0 13 14 13 661.3 0 13 15 11 661.3 0 11 16 13 661.3 0 13 17 11 661.3 0 11 18 17 661.3 0 17 19 9 661.3 0 9 20 12 661.3 0 12 21 15 661.3 0 15 22 14 661.3 0 14 23 8 661.3 0 8 24 11 661.3 0 11 25 13 661.3 0 13 26 9 661.3 0 9 27 10 661.3 0 10 28 15 661.3 0 15 29 15 661.3 0 15 30 6 661.3 0 6 31 15 661.3 0 15 32 45 661.3 0 45 33 12 661.3 0 12 34 19 661.3 0 19 35 26 661.3 0 26 36 9 661.3 0 9 37 19 661.3 0 19 38 10 661.3 0 10 39 17 661.3 0 17 40 13 661.3 0 13 41 17 661.3 0 17 42 13 661.3 0 13 43 10 661.3 0 10 44 21 661.3 0 21 45 18 661.3 0 18 46 17 661.3 0 17 47 10 661.3 0 10 48 9 661.3 0 9 49 9 661.3 0 9 50 9 661.3 0 9 51 14 661.3 0 14 52 22 661.3 0 22 53 15 661.3 0 15 54 21 661.3 0 21 55 28 661.3 0 28 56 23 661.3 0 23 57 11 661.3 0 11 58 6 661.3 0 6 59 15 661.3 0 15 60 14 661.3 0 14 61 15 661.3 0 15 62 14 661.3 0 14 63 36 661.3 0 36 64 13 661.3 0 13 65 18 661.3 0 18 66 15 661.3 0 15 67 23 661.3 0 23 68 26 661.3 0 26 69 258 661.3 0 258 70 37 661.3 0 37 71 64 661.3 0 64 72 41 661.3 0 41 73 41 661.3 0 41 74 247 661.3 0 247 75 32 661.3 0 32 76 142 661.3 0 142 77 17 661.3 0 17 78 17 661.3 0 17 79 22 661.3 0 22 80 29 661.3 0 29 81 67 661.3 0 67 82 189 661.3 0 189 83 158 661.3 0 158 84 510 661.3 0 510 85 26 661.3 0 26 86 20 661.3 0 20 87 19 661.3 0 19 88 28 661.3 0 28 89 25 661.3 0 25 90 21 661.3 0 21 91 14 661.3 0 14 92 46 661.3 0 46 93 57 661.3 0 57 94 27 661.3 0 27 95 19 661.3 0 19 96 26 661.3 0 26 97 28 661.3 0 28 98 28 661.3 0 28 99 22 661.3 0 22 100 28 661.3 0 28 101 41 661.3 0 41 102 24 661.3 0 24 103 26 661.3 0 26 104 21 661.3 0 21 105 9 661.3 0 9 106 26 661.3 0 26 107 14 661.3 0 14 108 14 661.3 0 14 109 28 661.3 0 28 110 26 661.3 0 26 111 20 661.3 0 20 112 30 661.3 0 30 113 22 661.3 0 22 114 25 661.3 0 25 115 25 661.3 0 25 116 26 661.3 0 26 117 35 661.3 0 35 118 50 661.3 0 50 119 45 661.3 0 45 120 37 661.3 0 37 121 44 661.3 0 44 122 55 661.3 0 55 123 89 661.3 0 89 124 33 661.3 0 33 125 43 661.3 0 43 126 52 661.3 0 52 127 88 661.3 0 88 128 71 661.3 0 71 129 40 661.3 0 40 130 37 661.3 0 37 131 43 661.3 0 43 132 67 661.3 0 67 133 59 661.3 0 59 134 44 661.3 0 44 135 49 661.3 0 49 136 34 661.3 0 34 137 60 661.3 0 60 138 51 661.3 0 51 139 93 661.3 0 93 140 52 661.3 0 52 141 30 661.3 0 30 142 48 661.3 0 48 143 50 661.3 0 50 144 65 661.3 0 65 145 49 661.3 0 49 146 43 661.3 0 43 147 46 661.3 0 46 148 46 661.3 0 46 149 57 661.3 0 57 150 51 661.3 0 51 151 53 661.3 0 53 152 69 661.3 0 69 153 64 661.3 0 64 154 74 661.3 0 74 155 71 661.3 0 71 156 56 661.3 0 56 157 68 661.3 0 68 158 41 661.3 0 41 159 59 661.3 0 59 160 51 661.3 0 51 161 50 661.3 0 50 162 72 661.3 0 72 163 92 661.3 0 92 164 93 661.3 0 93 165 82 661.3 0 82 166 111 661.3 0 111 167 490 661.3 0 490 168 228 661.3 0 228 169 631 661.3 0 631 170 93 661.3 0 93 171 585 661.3 0 585 172 243 661.3 0 243 173 198 661.3 0 198 174 93 661.3 0 93 175 71 661.3 0 71 176 61 661.3 0 61 177 55 661.3 0 55 178 72 661.3 0 72 179 81 661.3 0 81 180 67 661.3 0 67 181 80 661.3 0 80 182 72 661.3 0 72 183 99 661.3 0 99 184 125 661.3 0 125 185 62 661.3 0 62 186 68 661.3 0 68 187 60 661.3 0 60 188 88 661.3 0 88 189 196 661.3 0 196 190 112 661.3 0 112 191 90 661.3 0 90 192 65 661.3 0 65 193 52 661.3 0 52 194 73 661.3 0 73 195 71 661.3 0 71 196 105 661.3 0 105 197 63 661.3 0 63 198 94 661.3 0 94 199 91 661.3 0 91 200 227 661.3 0 227 201 644 661.3 0 644 202 275 661.3 0 275 203 136 661.3 0 136 204 79 661.3 0 79 205 94 661.3 0 94 206 87 661.3 0 87 207 78 661.3 0 78 208 81 661.3 0 81 209 69 661.3 0 69 210 71 661.3 0 71 211 104 661.3 0 104 212 77 661.3 0 77 213 91 661.3 0 91 214 96 661.3 0 96 215 117 661.3 0 117 216 141 661.3 0 141 217 111 661.3 0 111 218 96 661.3 0 96 219 97 661.3 0 97 220 86 661.3 0 86 221 81 661.3 0 81 222 111 661.3 0 111 223 119 661.3 0 119 224 141 661.3 0 141 225 197 661.3 0 197 226 264 661.3 0 264 227 845 661.3 0 845 228 4199 661.3 0 4199 229 1509 661.3 0 1509 230 2930 661.3 0 2930 231 189 661.3 0 189 232 88 661.3 0 88 233 97 661.3 0 97 234 88 661.3 0 88 235 75 661.3 0 75 236 94 661.3 0 94 237 69 661.3 0 69 238 84 661.3 0 84 239 94 661.3 0 94 240 84 661.3 0 84 241 68 661.3 0 68 242 72 661.3 0 72 243 130 661.3 0 130 244 115 661.3 0 115 245 97 661.3 0 97 246 83 661.3 0 83 247 102 661.3 0 102 248 55 661.3 0 55 249 77 661.3 0 77 250 133 661.3 0 133 251 168 661.3 0 168 252 99 661.3 0 99 253 91 661.3 0 91 254 92 661.3 0 92 255 138 661.3 0 138 256 239 661.3 0 239 257 246 661.3 0 246 258 467 661.3 0 467 259 112 661.3 0 112 260 100 661.3 0 100 261 168 661.3 0 168 262 271 661.3 0 271 263 329 661.3 0 329 264 184 661.3 0 184 265 191 661.3 0 191 266 92 661.3 0 92 267 87 661.3 0 87 268 215 661.3 0 215 269 695 661.3 0 695 270 1002 661.3 0 1002 271 158 661.3 0 158 272 87 661.3 0 87 273 93 661.3 0 93 274 78 661.3 0 78 275 78 661.3 0 78 276 88 661.3 0 88 277 104 661.3 0 104 278 78 661.3 0 78 279 82 661.3 0 82 280 86 661.3 0 86 281 96 661.3 0 96 282 169 661.3 0 169 283 119 661.3 0 119 284 108 661.3 0 108 285 80 661.3 0 80 286 71 661.3 0 71 287 81 661.3 0 81 288 88 661.3 0 88 289 98 661.3 0 98 290 72 661.3 0 72 291 108 661.3 0 108 292 73 661.3 0 73 293 86 661.3 0 86 294 65 661.3 0 65 295 58 661.3 0 58 296 50 661.3 0 50 297 61 661.3 0 61 298 100 661.3 0 100 299 165 661.3 0 165 300 302 661.3 0 302 301 86 661.3 0 86 Finished in 0.92 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 20,694 Reads with adapters: 131 (0.6%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 128 (0.6%) Total basepairs processed: 4,494,777 bp Total written (filtered): 22,220 bp (0.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 131 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 21 6 0.0 4 6 22 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 127 116 0.0 4 0 0 0 0 116 252 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 128 Reads with adapters: 0 (0.0%) Reads written (passing filters): 128 (100.0%) Total basepairs processed: 22,220 bp Total written (filtered): 22,220 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.