This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.16 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 301,436 (11.1%) Reads that were too short: 11,941 (0.4%) Reads written (passing filters): 289,495 (10.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 61,306,565 bp (7.5%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 301436 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3751 661.3 0 3751 7 77 661.3 0 77 8 85 661.3 0 85 9 984 661.3 0 984 10 58 661.3 0 58 11 45 661.3 0 45 12 48 661.3 0 48 13 52 661.3 0 52 14 55 661.3 0 55 15 64 661.3 0 64 16 58 661.3 0 58 17 53 661.3 0 53 18 45 661.3 0 45 19 41 661.3 0 41 20 46 661.3 0 46 21 72 661.3 0 72 22 62 661.3 0 62 23 47 661.3 0 47 24 73 661.3 0 73 25 40 661.3 0 40 26 48 661.3 0 48 27 55 661.3 0 55 28 60 661.3 0 60 29 41 661.3 0 41 30 52 661.3 0 52 31 55 661.3 0 55 32 46 661.3 0 46 33 56 661.3 0 56 34 41 661.3 0 41 35 44 661.3 0 44 36 62 661.3 0 62 37 56 661.3 0 56 38 42 661.3 0 42 39 50 661.3 0 50 40 58 661.3 0 58 41 66 661.3 0 66 42 44 661.3 0 44 43 49 661.3 0 49 44 61 661.3 0 61 45 81 661.3 0 81 46 61 661.3 0 61 47 52 661.3 0 52 48 48 661.3 0 48 49 44 661.3 0 44 50 32 661.3 0 32 51 77 661.3 0 77 52 62 661.3 0 62 53 45 661.3 0 45 54 89 661.3 0 89 55 78 661.3 0 78 56 69 661.3 0 69 57 95 661.3 0 95 58 70 661.3 0 70 59 66 661.3 0 66 60 91 661.3 0 91 61 69 661.3 0 69 62 133 661.3 0 133 63 68 661.3 0 68 64 85 661.3 0 85 65 74 661.3 0 74 66 163 661.3 0 163 67 127 661.3 0 127 68 215 661.3 0 215 69 431 661.3 0 431 70 407 661.3 0 407 71 501 661.3 0 501 72 1178 661.3 0 1178 73 2477 661.3 0 2477 74 944 661.3 0 944 75 837 661.3 0 837 76 1445 661.3 0 1445 77 747 661.3 0 747 78 789 661.3 0 789 79 1312 661.3 0 1312 80 1637 661.3 0 1637 81 2013 661.3 0 2013 82 1320 661.3 0 1320 83 1802 661.3 0 1802 84 1991 661.3 0 1991 85 3087 661.3 0 3087 86 29038 661.3 0 29038 87 86867 661.3 0 86867 88 119928 661.3 0 119928 89 814 661.3 0 814 90 63 661.3 0 63 91 52 661.3 0 52 92 51 661.3 0 51 93 82 661.3 0 82 94 106 661.3 0 106 95 82 661.3 0 82 96 55 661.3 0 55 97 43 661.3 0 43 98 48 661.3 0 48 99 73 661.3 0 73 100 138 661.3 0 138 101 73 661.3 0 73 102 53 661.3 0 53 103 41 661.3 0 41 104 38 661.3 0 38 105 48 661.3 0 48 106 41 661.3 0 41 107 35 661.3 0 35 108 56 661.3 0 56 109 66 661.3 0 66 110 58 661.3 0 58 111 58 661.3 0 58 112 59 661.3 0 59 113 62 661.3 0 62 114 71 661.3 0 71 115 72 661.3 0 72 116 79 661.3 0 79 117 73 661.3 0 73 118 60 661.3 0 60 119 64 661.3 0 64 120 69 661.3 0 69 121 92 661.3 0 92 122 102 661.3 0 102 123 268 661.3 0 268 124 463 661.3 0 463 125 1028 661.3 0 1028 126 398 661.3 0 398 127 852 661.3 0 852 128 247 661.3 0 247 129 162 661.3 0 162 130 113 661.3 0 113 131 160 661.3 0 160 132 84 661.3 0 84 133 98 661.3 0 98 134 125 661.3 0 125 135 96 661.3 0 96 136 99 661.3 0 99 137 112 661.3 0 112 138 89 661.3 0 89 139 106 661.3 0 106 140 103 661.3 0 103 141 126 661.3 0 126 142 151 661.3 0 151 143 180 661.3 0 180 144 754 661.3 0 754 145 3593 661.3 0 3593 146 2069 661.3 0 2069 147 265 661.3 0 265 148 73 661.3 0 73 149 50 661.3 0 50 150 89 661.3 0 89 151 73 661.3 0 73 152 71 661.3 0 71 153 51 661.3 0 51 154 83 661.3 0 83 155 95 661.3 0 95 156 96 661.3 0 96 157 94 661.3 0 94 158 80 661.3 0 80 159 58 661.3 0 58 160 75 661.3 0 75 161 71 661.3 0 71 162 59 661.3 0 59 163 74 661.3 0 74 164 64 661.3 0 64 165 79 661.3 0 79 166 396 661.3 0 396 167 301 661.3 0 301 168 503 661.3 0 503 169 84 661.3 0 84 170 109 661.3 0 109 171 150 661.3 0 150 172 93 661.3 0 93 173 93 661.3 0 93 174 73 661.3 0 73 175 98 661.3 0 98 176 57 661.3 0 57 177 68 661.3 0 68 178 67 661.3 0 67 179 57 661.3 0 57 180 95 661.3 0 95 181 91 661.3 0 91 182 88 661.3 0 88 183 93 661.3 0 93 184 101 661.3 0 101 185 256 661.3 0 256 186 1606 661.3 0 1606 187 277 661.3 0 277 188 117 661.3 0 117 189 80 661.3 0 80 190 68 661.3 0 68 191 99 661.3 0 99 192 74 661.3 0 74 193 95 661.3 0 95 194 71 661.3 0 71 195 71 661.3 0 71 196 85 661.3 0 85 197 83 661.3 0 83 198 118 661.3 0 118 199 103 661.3 0 103 200 64 661.3 0 64 201 71 661.3 0 71 202 76 661.3 0 76 203 108 661.3 0 108 204 134 661.3 0 134 205 156 661.3 0 156 206 476 661.3 0 476 207 1202 661.3 0 1202 208 816 661.3 0 816 209 96 661.3 0 96 210 115 661.3 0 115 211 269 661.3 0 269 212 475 661.3 0 475 213 539 661.3 0 539 214 76 661.3 0 76 215 63 661.3 0 63 216 66 661.3 0 66 217 57 661.3 0 57 218 53 661.3 0 53 219 58 661.3 0 58 220 53 661.3 0 53 221 47 661.3 0 47 222 84 661.3 0 84 223 95 661.3 0 95 224 67 661.3 0 67 225 70 661.3 0 70 226 73 661.3 0 73 227 110 661.3 0 110 228 93 661.3 0 93 229 59 661.3 0 59 230 47 661.3 0 47 231 57 661.3 0 57 232 45 661.3 0 45 233 58 661.3 0 58 234 65 661.3 0 65 235 63 661.3 0 63 236 68 661.3 0 68 237 51 661.3 0 51 238 60 661.3 0 60 239 69 661.3 0 69 240 52 661.3 0 52 241 217 661.3 0 217 242 490 661.3 0 490 243 521 661.3 0 521 244 63 661.3 0 63 245 59 661.3 0 59 246 65 661.3 0 65 247 39 661.3 0 39 248 38 661.3 0 38 249 36 661.3 0 36 250 59 661.3 0 59 251 125 661.3 0 125 252 143 661.3 0 143 253 83 661.3 0 83 254 55 661.3 0 55 255 78 661.3 0 78 256 188 661.3 0 188 257 58 661.3 0 58 258 36 661.3 0 36 259 34 661.3 0 34 260 55 661.3 0 55 261 106 661.3 0 106 262 123 661.3 0 123 263 152 661.3 0 152 264 140 661.3 0 140 265 131 661.3 0 131 266 85 661.3 0 85 267 71 661.3 0 71 268 55 661.3 0 55 269 31 661.3 0 31 270 49 661.3 0 49 271 58 661.3 0 58 272 76 661.3 0 76 273 55 661.3 0 55 274 42 661.3 0 42 275 50 661.3 0 50 276 65 661.3 0 65 277 164 661.3 0 164 278 128 661.3 0 128 279 111 661.3 0 111 280 46 661.3 0 46 281 48 661.3 0 48 282 62 661.3 0 62 283 97 661.3 0 97 284 62 661.3 0 62 285 52 661.3 0 52 286 41 661.3 0 41 287 36 661.3 0 36 288 48 661.3 0 48 289 41 661.3 0 41 290 77 661.3 0 77 291 93 661.3 0 93 292 66 661.3 0 66 293 49 661.3 0 49 294 40 661.3 0 40 295 65 661.3 0 65 296 142 661.3 0 142 297 78 661.3 0 78 298 78 661.3 0 78 299 90 661.3 0 90 300 89 661.3 0 89 301 186 661.3 0 186 Finished in 10.26 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 289,495 Reads with adapters: 6,727 (2.3%) Reads that were too short: 183 (0.1%) Reads written (passing filters): 6,544 (2.3%) Total basepairs processed: 61,306,565 bp Total written (filtered): 1,483,531 bp (2.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6727 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 4 0.0 3 0 2 1 1 17 948 0.0 3 921 23 4 18 6 0.0 3 0 2 2 2 19 42 0.0 3 3 34 0 5 20 3614 0.0 3 3528 70 11 5 21 10 0.0 3 1 5 3 1 22 4 0.0 3 0 0 1 3 23 6 0.0 3 0 0 0 6 24 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 66 24 0.0 3 0 0 0 24 68 1 0.0 3 0 0 0 1 69 6 0.0 3 0 0 0 6 72 1 0.0 3 0 0 0 1 80 9 0.0 3 0 0 0 9 81 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 0 3 83 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 104 5 0.0 3 0 0 0 5 105 1513 0.0 3 0 0 5 1508 106 137 0.0 3 0 0 0 137 107 1 0.0 3 0 0 0 1 108 188 0.0 3 0 0 8 180 118 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 158 8 0.0 3 0 0 0 8 164 2 0.0 3 0 1 1 165 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 167 5 0.0 3 0 0 0 5 168 5 0.0 3 0 0 0 5 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 6 0.0 3 0 0 0 6 205 1 0.0 3 0 0 0 1 206 70 0.0 3 0 0 0 70 207 57 0.0 3 0 0 3 54 208 4 0.0 3 0 0 0 4 212 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 6,544 Reads with adapters: 279 (4.3%) Reads written (passing filters): 6,544 (100.0%) Total basepairs processed: 1,483,531 bp Total written (filtered): 1,482,694 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 279 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.8% C: 12.2% G: 61.6% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 279 102.2 0 279