This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.32 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 55,945 (2.1%) Reads that were too short: 15,349 (0.6%) Reads written (passing filters): 40,596 (1.5%) Total basepairs processed: 815,312,680 bp Total written (filtered): 8,049,261 bp (1.0%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 55945 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 178 661.3 0 178 7 113 661.3 0 113 8 2229 661.3 0 2229 9 83 661.3 0 83 10 66 661.3 0 66 11 68 661.3 0 68 12 70 661.3 0 70 13 98 661.3 0 98 14 81 661.3 0 81 15 73 661.3 0 73 16 72 661.3 0 72 17 82 661.3 0 82 18 101 661.3 0 101 19 99 661.3 0 99 20 67 661.3 0 67 21 80 661.3 0 80 22 47 661.3 0 47 23 112 661.3 0 112 24 123 661.3 0 123 25 83 661.3 0 83 26 90 661.3 0 90 27 114 661.3 0 114 28 98 661.3 0 98 29 72 661.3 0 72 30 82 661.3 0 82 31 85 661.3 0 85 32 146 661.3 0 146 33 113 661.3 0 113 34 111 661.3 0 111 35 118 661.3 0 118 36 134 661.3 0 134 37 732 661.3 0 732 38 844 661.3 0 844 39 2888 661.3 0 2888 40 675 661.3 0 675 41 85 661.3 0 85 42 98 661.3 0 98 43 85 661.3 0 85 44 130 661.3 0 130 45 95 661.3 0 95 46 84 661.3 0 84 47 89 661.3 0 89 48 91 661.3 0 91 49 91 661.3 0 91 50 72 661.3 0 72 51 99 661.3 0 99 52 112 661.3 0 112 53 71 661.3 0 71 54 92 661.3 0 92 55 84 661.3 0 84 56 95 661.3 0 95 57 65 661.3 0 65 58 90 661.3 0 90 59 88 661.3 0 88 60 84 661.3 0 84 61 86 661.3 0 86 62 72 661.3 0 72 63 87 661.3 0 87 64 90 661.3 0 90 65 92 661.3 0 92 66 83 661.3 0 83 67 89 661.3 0 89 68 86 661.3 0 86 69 110 661.3 0 110 70 121 661.3 0 121 71 111 661.3 0 111 72 92 661.3 0 92 73 82 661.3 0 82 74 79 661.3 0 79 75 77 661.3 0 77 76 145 661.3 0 145 77 196 661.3 0 196 78 103 661.3 0 103 79 128 661.3 0 128 80 80 661.3 0 80 81 105 661.3 0 105 82 105 661.3 0 105 83 87 661.3 0 87 84 98 661.3 0 98 85 60 661.3 0 60 86 88 661.3 0 88 87 86 661.3 0 86 88 108 661.3 0 108 89 141 661.3 0 141 90 99 661.3 0 99 91 550 661.3 0 550 92 437 661.3 0 437 93 402 661.3 0 402 94 121 661.3 0 121 95 95 661.3 0 95 96 78 661.3 0 78 97 86 661.3 0 86 98 62 661.3 0 62 99 66 661.3 0 66 100 79 661.3 0 79 101 97 661.3 0 97 102 79 661.3 0 79 103 78 661.3 0 78 104 99 661.3 0 99 105 75 661.3 0 75 106 80 661.3 0 80 107 103 661.3 0 103 108 81 661.3 0 81 109 65 661.3 0 65 110 99 661.3 0 99 111 80 661.3 0 80 112 127 661.3 0 127 113 124 661.3 0 124 114 175 661.3 0 175 115 136 661.3 0 136 116 141 661.3 0 141 117 83 661.3 0 83 118 115 661.3 0 115 119 84 661.3 0 84 120 71 661.3 0 71 121 96 661.3 0 96 122 126 661.3 0 126 123 94 661.3 0 94 124 87 661.3 0 87 125 96 661.3 0 96 126 94 661.3 0 94 127 107 661.3 0 107 128 189 661.3 0 189 129 535 661.3 0 535 130 485 661.3 0 485 131 597 661.3 0 597 132 154 661.3 0 154 133 153 661.3 0 153 134 124 661.3 0 124 135 125 661.3 0 125 136 199 661.3 0 199 137 177 661.3 0 177 138 161 661.3 0 161 139 203 661.3 0 203 140 227 661.3 0 227 141 250 661.3 0 250 142 3271 661.3 0 3271 143 4974 661.3 0 4974 144 4579 661.3 0 4579 145 89 661.3 0 89 146 98 661.3 0 98 147 84 661.3 0 84 148 77 661.3 0 77 149 117 661.3 0 117 150 169 661.3 0 169 151 189 661.3 0 189 152 71 661.3 0 71 153 92 661.3 0 92 154 81 661.3 0 81 155 75 661.3 0 75 156 99 661.3 0 99 157 84 661.3 0 84 158 96 661.3 0 96 159 75 661.3 0 75 160 78 661.3 0 78 161 70 661.3 0 70 162 73 661.3 0 73 163 79 661.3 0 79 164 78 661.3 0 78 165 81 661.3 0 81 166 92 661.3 0 92 167 73 661.3 0 73 168 114 661.3 0 114 169 85 661.3 0 85 170 73 661.3 0 73 171 59 661.3 0 59 172 48 661.3 0 48 173 74 661.3 0 74 174 70 661.3 0 70 175 64 661.3 0 64 176 79 661.3 0 79 177 84 661.3 0 84 178 83 661.3 0 83 179 65 661.3 0 65 180 61 661.3 0 61 181 94 661.3 0 94 182 86 661.3 0 86 183 67 661.3 0 67 184 58 661.3 0 58 185 60 661.3 0 60 186 84 661.3 0 84 187 66 661.3 0 66 188 83 661.3 0 83 189 59 661.3 0 59 190 65 661.3 0 65 191 77 661.3 0 77 192 63 661.3 0 63 193 49 661.3 0 49 194 71 661.3 0 71 195 63 661.3 0 63 196 75 661.3 0 75 197 70 661.3 0 70 198 49 661.3 0 49 199 46 661.3 0 46 200 60 661.3 0 60 201 88 661.3 0 88 202 75 661.3 0 75 203 69 661.3 0 69 204 80 661.3 0 80 205 95 661.3 0 95 206 76 661.3 0 76 207 68 661.3 0 68 208 71 661.3 0 71 209 103 661.3 0 103 210 186 661.3 0 186 211 124 661.3 0 124 212 77 661.3 0 77 213 130 661.3 0 130 214 289 661.3 0 289 215 480 661.3 0 480 216 865 661.3 0 865 217 179 661.3 0 179 218 268 661.3 0 268 219 66 661.3 0 66 220 82 661.3 0 82 221 114 661.3 0 114 222 97 661.3 0 97 223 75 661.3 0 75 224 103 661.3 0 103 225 110 661.3 0 110 226 95 661.3 0 95 227 126 661.3 0 126 228 186 661.3 0 186 229 232 661.3 0 232 230 324 661.3 0 324 231 232 661.3 0 232 232 105 661.3 0 105 233 128 661.3 0 128 234 370 661.3 0 370 235 1309 661.3 0 1309 236 3639 661.3 0 3639 237 791 661.3 0 791 238 66 661.3 0 66 239 58 661.3 0 58 240 49 661.3 0 49 241 51 661.3 0 51 242 43 661.3 0 43 243 69 661.3 0 69 244 62 661.3 0 62 245 79 661.3 0 79 246 42 661.3 0 42 247 64 661.3 0 64 248 59 661.3 0 59 249 49 661.3 0 49 250 62 661.3 0 62 251 56 661.3 0 56 252 39 661.3 0 39 253 56 661.3 0 56 254 103 661.3 0 103 255 104 661.3 0 104 256 62 661.3 0 62 257 49 661.3 0 49 258 56 661.3 0 56 259 58 661.3 0 58 260 71 661.3 0 71 261 63 661.3 0 63 262 78 661.3 0 78 263 152 661.3 0 152 264 181 661.3 0 181 265 76 661.3 0 76 266 96 661.3 0 96 267 86 661.3 0 86 268 46 661.3 0 46 269 48 661.3 0 48 270 84 661.3 0 84 271 60 661.3 0 60 272 76 661.3 0 76 273 39 661.3 0 39 274 55 661.3 0 55 275 113 661.3 0 113 276 63 661.3 0 63 277 44 661.3 0 44 278 33 661.3 0 33 279 45 661.3 0 45 280 47 661.3 0 47 281 67 661.3 0 67 282 37 661.3 0 37 283 66 661.3 0 66 284 63 661.3 0 63 285 54 661.3 0 54 286 32 661.3 0 32 287 37 661.3 0 37 288 32 661.3 0 32 289 26 661.3 0 26 290 31 661.3 0 31 291 71 661.3 0 71 292 55 661.3 0 55 293 90 661.3 0 90 294 43 661.3 0 43 295 56 661.3 0 56 296 58 661.3 0 58 297 52 661.3 0 52 298 57 661.3 0 57 299 35 661.3 0 35 300 42 661.3 0 42 301 34 661.3 0 34 Finished in 1.50 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 40,596 Reads with adapters: 2,348 (5.8%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 2,338 (5.8%) Total basepairs processed: 8,049,261 bp Total written (filtered): 641,114 bp (8.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2348 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 31 0.0 3 0 26 3 2 17 2150 0.0 3 2080 63 3 4 18 18 0.0 3 1 5 7 5 19 14 0.0 3 0 2 0 12 20 98 0.0 3 90 2 1 5 22 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 45 2 0.0 3 0 0 0 2 50 4 0.0 3 0 0 0 4 63 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 5 0.0 3 0 0 0 5 167 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 1 1 249 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 2,338 Reads with adapters: 41 (1.8%) Reads written (passing filters): 2,338 (100.0%) Total basepairs processed: 641,114 bp Total written (filtered): 640,989 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 41 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.0% C: 29.3% G: 7.3% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 40 36.5 0 40 5 1 2.3 0 1