This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.35 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 184,254 (6.8%) Reads that were too short: 66,343 (2.4%) Reads written (passing filters): 117,911 (4.4%) Total basepairs processed: 815,312,680 bp Total written (filtered): 22,046,954 bp (2.7%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 184254 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 176 661.3 0 176 7 6495 661.3 0 6495 8 6613 661.3 0 6613 9 25 661.3 0 25 10 16 661.3 0 16 11 28 661.3 0 28 12 36 661.3 0 36 13 25 661.3 0 25 14 19 661.3 0 19 15 19 661.3 0 19 16 25 661.3 0 25 17 28 661.3 0 28 18 33 661.3 0 33 19 17 661.3 0 17 20 31 661.3 0 31 21 27 661.3 0 27 22 29 661.3 0 29 23 29 661.3 0 29 24 32 661.3 0 32 25 29 661.3 0 29 26 40 661.3 0 40 27 71 661.3 0 71 28 183 661.3 0 183 29 2326 661.3 0 2326 30 245 661.3 0 245 31 64 661.3 0 64 32 15 661.3 0 15 33 17 661.3 0 17 34 44 661.3 0 44 35 22 661.3 0 22 36 42 661.3 0 42 37 33 661.3 0 33 38 26 661.3 0 26 39 34 661.3 0 34 40 35 661.3 0 35 41 22 661.3 0 22 42 25 661.3 0 25 43 31 661.3 0 31 44 31 661.3 0 31 45 33 661.3 0 33 46 22 661.3 0 22 47 35 661.3 0 35 48 37 661.3 0 37 49 36 661.3 0 36 50 34 661.3 0 34 51 17 661.3 0 17 52 27 661.3 0 27 53 32 661.3 0 32 54 25 661.3 0 25 55 27 661.3 0 27 56 27 661.3 0 27 57 24 661.3 0 24 58 26 661.3 0 26 59 22 661.3 0 22 60 33 661.3 0 33 61 21 661.3 0 21 62 34 661.3 0 34 63 27 661.3 0 27 64 26 661.3 0 26 65 38 661.3 0 38 66 50 661.3 0 50 67 36 661.3 0 36 68 55 661.3 0 55 69 121 661.3 0 121 70 1180 661.3 0 1180 71 549 661.3 0 549 72 855 661.3 0 855 73 32 661.3 0 32 74 22 661.3 0 22 75 21 661.3 0 21 76 35 661.3 0 35 77 31 661.3 0 31 78 31 661.3 0 31 79 38 661.3 0 38 80 32 661.3 0 32 81 382 661.3 0 382 82 374 661.3 0 374 83 474 661.3 0 474 84 133 661.3 0 133 85 150 661.3 0 150 86 65 661.3 0 65 87 54 661.3 0 54 88 40 661.3 0 40 89 49 661.3 0 49 90 61 661.3 0 61 91 80 661.3 0 80 92 179 661.3 0 179 93 211 661.3 0 211 94 330 661.3 0 330 95 218 661.3 0 218 96 177 661.3 0 177 97 127 661.3 0 127 98 342 661.3 0 342 99 750 661.3 0 750 100 449 661.3 0 449 101 208 661.3 0 208 102 254 661.3 0 254 103 136 661.3 0 136 104 98 661.3 0 98 105 72 661.3 0 72 106 118 661.3 0 118 107 651 661.3 0 651 108 366 661.3 0 366 109 252 661.3 0 252 110 374 661.3 0 374 111 640 661.3 0 640 112 212 661.3 0 212 113 340 661.3 0 340 114 610 661.3 0 610 115 622 661.3 0 622 116 560 661.3 0 560 117 439 661.3 0 439 118 410 661.3 0 410 119 494 661.3 0 494 120 427 661.3 0 427 121 431 661.3 0 431 122 481 661.3 0 481 123 729 661.3 0 729 124 8350 661.3 0 8350 125 25751 661.3 0 25751 126 19714 661.3 0 19714 127 530 661.3 0 530 128 369 661.3 0 369 129 583 661.3 0 583 130 519 661.3 0 519 131 350 661.3 0 350 132 181 661.3 0 181 133 289 661.3 0 289 134 314 661.3 0 314 135 332 661.3 0 332 136 416 661.3 0 416 137 365 661.3 0 365 138 269 661.3 0 269 139 509 661.3 0 509 140 568 661.3 0 568 141 316 661.3 0 316 142 278 661.3 0 278 143 281 661.3 0 281 144 310 661.3 0 310 145 338 661.3 0 338 146 314 661.3 0 314 147 253 661.3 0 253 148 328 661.3 0 328 149 991 661.3 0 991 150 1409 661.3 0 1409 151 2520 661.3 0 2520 152 472 661.3 0 472 153 289 661.3 0 289 154 292 661.3 0 292 155 297 661.3 0 297 156 447 661.3 0 447 157 426 661.3 0 426 158 328 661.3 0 328 159 326 661.3 0 326 160 300 661.3 0 300 161 278 661.3 0 278 162 319 661.3 0 319 163 366 661.3 0 366 164 520 661.3 0 520 165 616 661.3 0 616 166 340 661.3 0 340 167 249 661.3 0 249 168 318 661.3 0 318 169 448 661.3 0 448 170 323 661.3 0 323 171 248 661.3 0 248 172 243 661.3 0 243 173 294 661.3 0 294 174 221 661.3 0 221 175 239 661.3 0 239 176 273 661.3 0 273 177 305 661.3 0 305 178 322 661.3 0 322 179 309 661.3 0 309 180 254 661.3 0 254 181 462 661.3 0 462 182 410 661.3 0 410 183 442 661.3 0 442 184 339 661.3 0 339 185 428 661.3 0 428 186 334 661.3 0 334 187 264 661.3 0 264 188 296 661.3 0 296 189 308 661.3 0 308 190 411 661.3 0 411 191 330 661.3 0 330 192 312 661.3 0 312 193 351 661.3 0 351 194 208 661.3 0 208 195 256 661.3 0 256 196 517 661.3 0 517 197 480 661.3 0 480 198 370 661.3 0 370 199 185 661.3 0 185 200 157 661.3 0 157 201 344 661.3 0 344 202 455 661.3 0 455 203 364 661.3 0 364 204 376 661.3 0 376 205 438 661.3 0 438 206 439 661.3 0 439 207 467 661.3 0 467 208 447 661.3 0 447 209 503 661.3 0 503 210 525 661.3 0 525 211 403 661.3 0 403 212 408 661.3 0 408 213 355 661.3 0 355 214 492 661.3 0 492 215 421 661.3 0 421 216 474 661.3 0 474 217 427 661.3 0 427 218 411 661.3 0 411 219 396 661.3 0 396 220 339 661.3 0 339 221 474 661.3 0 474 222 656 661.3 0 656 223 1031 661.3 0 1031 224 1331 661.3 0 1331 225 9930 661.3 0 9930 226 3341 661.3 0 3341 227 12263 661.3 0 12263 228 405 661.3 0 405 229 309 661.3 0 309 230 319 661.3 0 319 231 336 661.3 0 336 232 356 661.3 0 356 233 381 661.3 0 381 234 639 661.3 0 639 235 596 661.3 0 596 236 503 661.3 0 503 237 497 661.3 0 497 238 458 661.3 0 458 239 379 661.3 0 379 240 288 661.3 0 288 241 372 661.3 0 372 242 392 661.3 0 392 243 326 661.3 0 326 244 345 661.3 0 345 245 463 661.3 0 463 246 530 661.3 0 530 247 327 661.3 0 327 248 184 661.3 0 184 249 167 661.3 0 167 250 296 661.3 0 296 251 464 661.3 0 464 252 367 661.3 0 367 253 233 661.3 0 233 254 347 661.3 0 347 255 389 661.3 0 389 256 413 661.3 0 413 257 439 661.3 0 439 258 365 661.3 0 365 259 293 661.3 0 293 260 443 661.3 0 443 261 537 661.3 0 537 262 409 661.3 0 409 263 361 661.3 0 361 264 325 661.3 0 325 265 319 661.3 0 319 266 468 661.3 0 468 267 401 661.3 0 401 268 230 661.3 0 230 269 213 661.3 0 213 270 588 661.3 0 588 271 517 661.3 0 517 272 362 661.3 0 362 273 364 661.3 0 364 274 385 661.3 0 385 275 247 661.3 0 247 276 275 661.3 0 275 277 361 661.3 0 361 278 390 661.3 0 390 279 389 661.3 0 389 280 222 661.3 0 222 281 297 661.3 0 297 282 484 661.3 0 484 283 441 661.3 0 441 284 325 661.3 0 325 285 479 661.3 0 479 286 458 661.3 0 458 287 278 661.3 0 278 288 247 661.3 0 247 289 335 661.3 0 335 290 469 661.3 0 469 291 510 661.3 0 510 292 350 661.3 0 350 293 384 661.3 0 384 294 338 661.3 0 338 295 423 661.3 0 423 296 639 661.3 0 639 297 636 661.3 0 636 298 695 661.3 0 695 299 540 661.3 0 540 300 399 661.3 0 399 301 466 661.3 0 466 Finished in 4.66 s (40 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 117,911 Reads with adapters: 6,943 (5.9%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 6,918 (5.9%) Total basepairs processed: 22,046,954 bp Total written (filtered): 1,879,144 bp (8.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6943 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 1 5 18 2 0.0 3 0 0 0 0 2 19 28 0.0 3 0 25 3 20 2212 0.0 4 2120 88 1 0 3 21 4530 0.0 4 4480 46 3 1 22 14 0.0 4 3 8 0 3 23 3 0.0 4 0 0 0 2 1 25 75 0.0 4 0 0 0 0 75 26 4 0.0 4 0 0 0 0 4 27 19 0.0 4 16 2 1 29 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 40 2 0.0 4 0 0 0 1 1 43 3 0.0 4 2 0 0 0 1 44 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 1 110 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 2 121 1 0.0 4 0 0 0 0 1 127 16 0.0 4 0 0 0 0 16 140 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 6,918 Reads with adapters: 217 (3.1%) Reads written (passing filters): 6,918 (100.0%) Total basepairs processed: 1,879,144 bp Total written (filtered): 1,866,118 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 217 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 6.0% G: 76.0% T: 15.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 23 0.0 0 2 21 10 44 0.0 1 1 43 11 105 0.0 1 0 105 150 1 0.0 1 0 1 151 1 0.0 1 0 1 218 2 0.0 1 1 1 240 17 0.0 1 15 2 257 1 0.0 1 1 260 1 0.0 1 1 263 5 0.0 1 3 2 269 15 0.0 1 14 1 270 2 0.0 1 2