This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.38 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 87,334 (2.9%) Reads that were too short: 13,633 (0.5%) Reads written (passing filters): 73,701 (2.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 19,389,015 bp (2.1%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 87334 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10621 734.8 0 10621 7 6912 734.8 0 6912 8 35882 734.8 0 35882 9 67 734.8 0 67 10 23 734.8 0 23 11 20 734.8 0 20 12 19 734.8 0 19 13 28 734.8 0 28 14 20 734.8 0 20 15 21 734.8 0 21 16 18 734.8 0 18 17 20 734.8 0 20 18 51 734.8 0 51 19 24 734.8 0 24 20 65 734.8 0 65 21 40 734.8 0 40 22 34 734.8 0 34 23 24 734.8 0 24 24 8 734.8 0 8 25 18 734.8 0 18 26 26 734.8 0 26 27 52 734.8 0 52 28 38 734.8 0 38 29 1740 734.8 0 1740 30 121 734.8 0 121 31 516 734.8 0 516 32 81 734.8 0 81 33 23 734.8 0 23 34 29 734.8 0 29 35 14 734.8 0 14 36 22 734.8 0 22 37 18 734.8 0 18 38 22 734.8 0 22 39 21 734.8 0 21 40 28 734.8 0 28 41 23 734.8 0 23 42 10 734.8 0 10 43 26 734.8 0 26 44 23 734.8 0 23 45 30 734.8 0 30 46 26 734.8 0 26 47 33 734.8 0 33 48 23 734.8 0 23 49 15 734.8 0 15 50 29 734.8 0 29 51 25 734.8 0 25 52 35 734.8 0 35 53 23 734.8 0 23 54 57 734.8 0 57 55 57 734.8 0 57 56 45 734.8 0 45 57 14 734.8 0 14 58 21 734.8 0 21 59 20 734.8 0 20 60 24 734.8 0 24 61 115 734.8 0 115 62 185 734.8 0 185 63 48 734.8 0 48 64 31 734.8 0 31 65 15 734.8 0 15 66 16 734.8 0 16 67 29 734.8 0 29 68 53 734.8 0 53 69 31 734.8 0 31 70 22 734.8 0 22 71 46 734.8 0 46 72 38 734.8 0 38 73 29 734.8 0 29 74 80 734.8 0 80 75 46 734.8 0 46 76 144 734.8 0 144 77 124 734.8 0 124 78 132 734.8 0 132 79 87 734.8 0 87 80 86 734.8 0 86 81 89 734.8 0 89 82 86 734.8 0 86 83 130 734.8 0 130 84 132 734.8 0 132 85 73 734.8 0 73 86 118 734.8 0 118 87 131 734.8 0 131 88 52 734.8 0 52 89 82 734.8 0 82 90 80 734.8 0 80 91 59 734.8 0 59 92 172 734.8 0 172 93 142 734.8 0 142 94 106 734.8 0 106 95 167 734.8 0 167 96 90 734.8 0 90 97 108 734.8 0 108 98 138 734.8 0 138 99 168 734.8 0 168 100 158 734.8 0 158 101 58 734.8 0 58 102 30 734.8 0 30 103 35 734.8 0 35 104 18 734.8 0 18 105 32 734.8 0 32 106 11 734.8 0 11 107 25 734.8 0 25 108 47 734.8 0 47 109 56 734.8 0 56 110 75 734.8 0 75 111 82 734.8 0 82 112 143 734.8 0 143 113 94 734.8 0 94 114 64 734.8 0 64 115 73 734.8 0 73 116 73 734.8 0 73 117 52 734.8 0 52 118 44 734.8 0 44 119 77 734.8 0 77 120 85 734.8 0 85 121 83 734.8 0 83 122 90 734.8 0 90 123 132 734.8 0 132 124 111 734.8 0 111 125 115 734.8 0 115 126 262 734.8 0 262 127 188 734.8 0 188 128 1070 734.8 0 1070 129 767 734.8 0 767 130 380 734.8 0 380 131 130 734.8 0 130 132 90 734.8 0 90 133 93 734.8 0 93 134 112 734.8 0 112 135 127 734.8 0 127 136 120 734.8 0 120 137 85 734.8 0 85 138 93 734.8 0 93 139 92 734.8 0 92 140 109 734.8 0 109 141 83 734.8 0 83 142 79 734.8 0 79 143 81 734.8 0 81 144 121 734.8 0 121 145 145 734.8 0 145 146 98 734.8 0 98 147 106 734.8 0 106 148 91 734.8 0 91 149 99 734.8 0 99 150 67 734.8 0 67 151 80 734.8 0 80 152 108 734.8 0 108 153 88 734.8 0 88 154 112 734.8 0 112 155 108 734.8 0 108 156 97 734.8 0 97 157 143 734.8 0 143 158 100 734.8 0 100 159 82 734.8 0 82 160 105 734.8 0 105 161 76 734.8 0 76 162 82 734.8 0 82 163 101 734.8 0 101 164 80 734.8 0 80 165 138 734.8 0 138 166 310 734.8 0 310 167 238 734.8 0 238 168 154 734.8 0 154 169 90 734.8 0 90 170 64 734.8 0 64 171 80 734.8 0 80 172 94 734.8 0 94 173 83 734.8 0 83 174 121 734.8 0 121 175 135 734.8 0 135 176 102 734.8 0 102 177 99 734.8 0 99 178 112 734.8 0 112 179 86 734.8 0 86 180 95 734.8 0 95 181 86 734.8 0 86 182 125 734.8 0 125 183 133 734.8 0 133 184 126 734.8 0 126 185 125 734.8 0 125 186 119 734.8 0 119 187 90 734.8 0 90 188 50 734.8 0 50 189 64 734.8 0 64 190 169 734.8 0 169 191 211 734.8 0 211 192 194 734.8 0 194 193 129 734.8 0 129 194 110 734.8 0 110 195 167 734.8 0 167 196 173 734.8 0 173 197 432 734.8 0 432 198 297 734.8 0 297 199 129 734.8 0 129 200 71 734.8 0 71 201 82 734.8 0 82 202 161 734.8 0 161 203 202 734.8 0 202 204 139 734.8 0 139 205 125 734.8 0 125 206 100 734.8 0 100 207 106 734.8 0 106 208 111 734.8 0 111 209 107 734.8 0 107 210 174 734.8 0 174 211 330 734.8 0 330 212 270 734.8 0 270 213 195 734.8 0 195 214 124 734.8 0 124 215 128 734.8 0 128 216 144 734.8 0 144 217 120 734.8 0 120 218 244 734.8 0 244 219 212 734.8 0 212 220 180 734.8 0 180 221 231 734.8 0 231 222 149 734.8 0 149 223 223 734.8 0 223 224 134 734.8 0 134 225 141 734.8 0 141 226 126 734.8 0 126 227 91 734.8 0 91 228 87 734.8 0 87 229 86 734.8 0 86 230 84 734.8 0 84 231 96 734.8 0 96 232 133 734.8 0 133 233 185 734.8 0 185 234 173 734.8 0 173 235 133 734.8 0 133 236 108 734.8 0 108 237 95 734.8 0 95 238 181 734.8 0 181 239 172 734.8 0 172 240 195 734.8 0 195 241 200 734.8 0 200 242 251 734.8 0 251 243 261 734.8 0 261 244 163 734.8 0 163 245 156 734.8 0 156 246 155 734.8 0 155 247 70 734.8 0 70 248 39 734.8 0 39 249 71 734.8 0 71 250 146 734.8 0 146 251 243 734.8 0 243 252 336 734.8 0 336 253 269 734.8 0 269 254 150 734.8 0 150 255 175 734.8 0 175 256 102 734.8 0 102 257 55 734.8 0 55 258 48 734.8 0 48 259 41 734.8 0 41 260 49 734.8 0 49 261 65 734.8 0 65 262 257 734.8 0 257 263 260 734.8 0 260 264 245 734.8 0 245 265 162 734.8 0 162 266 109 734.8 0 109 267 216 734.8 0 216 268 97 734.8 0 97 269 64 734.8 0 64 270 73 734.8 0 73 271 157 734.8 0 157 272 274 734.8 0 274 273 191 734.8 0 191 274 115 734.8 0 115 275 61 734.8 0 61 276 58 734.8 0 58 277 95 734.8 0 95 278 194 734.8 0 194 279 139 734.8 0 139 280 43 734.8 0 43 281 47 734.8 0 47 282 74 734.8 0 74 283 115 734.8 0 115 284 145 734.8 0 145 285 81 734.8 0 81 286 76 734.8 0 76 287 42 734.8 0 42 288 39 734.8 0 39 289 65 734.8 0 65 290 122 734.8 0 122 291 166 734.8 0 166 292 123 734.8 0 123 293 68 734.8 0 68 294 51 734.8 0 51 295 45 734.8 0 45 296 45 734.8 0 45 297 71 734.8 0 71 298 81 734.8 0 81 299 66 734.8 0 66 300 169 734.8 0 169 301 92 734.8 0 92 Finished in 3.33 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 73,701 Reads with adapters: 16,811 (22.8%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 16,785 (22.8%) Total basepairs processed: 19,389,015 bp Total written (filtered): 4,638,613 bp (23.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16811 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 75 0.0 3 0 63 10 2 17 6412 0.0 3 6121 262 21 8 18 10180 0.0 3 9880 248 39 13 19 21 0.0 3 0 17 3 1 20 16 0.0 3 1 1 8 6 21 11 0.0 3 0 0 0 11 22 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 42 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 1 63 27 0.0 3 0 0 1 26 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 17 0.0 3 0 0 1 16 69 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 176 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 1 192 7 0.0 3 0 0 0 7 269 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.59 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 16,785 Reads with adapters: 1,089 (6.5%) Reads written (passing filters): 16,785 (100.0%) Total basepairs processed: 4,638,613 bp Total written (filtered): 4,635,337 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1089 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.5% C: 46.1% G: 13.5% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1082 262.3 0 1082 4 5 65.6 0 5 5 2 16.4 0 2