This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.73 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 21,492 (0.7%) Reads that were too short: 4,555 (0.2%) Reads written (passing filters): 16,937 (0.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 4,143,568 bp (0.5%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 21492 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 361 734.8 0 361 7 6603 734.8 0 6603 8 28 734.8 0 28 9 28 734.8 0 28 10 18 734.8 0 18 11 18 734.8 0 18 12 14 734.8 0 14 13 27 734.8 0 27 14 10 734.8 0 10 15 19 734.8 0 19 16 23 734.8 0 23 17 22 734.8 0 22 18 21 734.8 0 21 19 22 734.8 0 22 20 23 734.8 0 23 21 33 734.8 0 33 22 44 734.8 0 44 23 509 734.8 0 509 24 580 734.8 0 580 25 125 734.8 0 125 26 17 734.8 0 17 27 7 734.8 0 7 28 7 734.8 0 7 29 14 734.8 0 14 30 14 734.8 0 14 31 30 734.8 0 30 32 17 734.8 0 17 33 21 734.8 0 21 34 17 734.8 0 17 35 22 734.8 0 22 36 13 734.8 0 13 37 16 734.8 0 16 38 13 734.8 0 13 39 17 734.8 0 17 40 17 734.8 0 17 41 13 734.8 0 13 42 16 734.8 0 16 43 13 734.8 0 13 44 12 734.8 0 12 45 15 734.8 0 15 46 12 734.8 0 12 47 6 734.8 0 6 48 9 734.8 0 9 49 17 734.8 0 17 50 9 734.8 0 9 51 6 734.8 0 6 52 11 734.8 0 11 53 11 734.8 0 11 54 10 734.8 0 10 55 15 734.8 0 15 56 23 734.8 0 23 57 56 734.8 0 56 58 14 734.8 0 14 59 25 734.8 0 25 60 20 734.8 0 20 61 13 734.8 0 13 62 8 734.8 0 8 63 25 734.8 0 25 64 12 734.8 0 12 65 12 734.8 0 12 66 14 734.8 0 14 67 18 734.8 0 18 68 17 734.8 0 17 69 31 734.8 0 31 70 27 734.8 0 27 71 14 734.8 0 14 72 12 734.8 0 12 73 9 734.8 0 9 74 40 734.8 0 40 75 39 734.8 0 39 76 16 734.8 0 16 77 14 734.8 0 14 78 30 734.8 0 30 79 19 734.8 0 19 80 25 734.8 0 25 81 29 734.8 0 29 82 43 734.8 0 43 83 29 734.8 0 29 84 34 734.8 0 34 85 35 734.8 0 35 86 35 734.8 0 35 87 73 734.8 0 73 88 2091 734.8 0 2091 89 1192 734.8 0 1192 90 346 734.8 0 346 91 10 734.8 0 10 92 14 734.8 0 14 93 11 734.8 0 11 94 8 734.8 0 8 95 27 734.8 0 27 96 68 734.8 0 68 97 50 734.8 0 50 98 19 734.8 0 19 99 24 734.8 0 24 100 23 734.8 0 23 101 204 734.8 0 204 102 415 734.8 0 415 103 131 734.8 0 131 104 30 734.8 0 30 105 36 734.8 0 36 106 8 734.8 0 8 107 10 734.8 0 10 108 7 734.8 0 7 109 17 734.8 0 17 110 22 734.8 0 22 111 20 734.8 0 20 112 17 734.8 0 17 113 23 734.8 0 23 114 9 734.8 0 9 115 15 734.8 0 15 116 7 734.8 0 7 117 10 734.8 0 10 118 18 734.8 0 18 119 19 734.8 0 19 120 16 734.8 0 16 121 5 734.8 0 5 122 15 734.8 0 15 123 11 734.8 0 11 124 19 734.8 0 19 125 17 734.8 0 17 126 34 734.8 0 34 127 528 734.8 0 528 128 250 734.8 0 250 129 85 734.8 0 85 130 13 734.8 0 13 131 14 734.8 0 14 132 9 734.8 0 9 133 27 734.8 0 27 134 13 734.8 0 13 135 18 734.8 0 18 136 9 734.8 0 9 137 11 734.8 0 11 138 11 734.8 0 11 139 16 734.8 0 16 140 7 734.8 0 7 141 17 734.8 0 17 142 20 734.8 0 20 143 10 734.8 0 10 144 17 734.8 0 17 145 16 734.8 0 16 146 16 734.8 0 16 147 15 734.8 0 15 148 40 734.8 0 40 149 24 734.8 0 24 150 3 734.8 0 3 151 11 734.8 0 11 152 9 734.8 0 9 153 10 734.8 0 10 154 16 734.8 0 16 155 8 734.8 0 8 156 21 734.8 0 21 157 11 734.8 0 11 158 7 734.8 0 7 159 10 734.8 0 10 160 9 734.8 0 9 161 16 734.8 0 16 162 13 734.8 0 13 163 13 734.8 0 13 164 11 734.8 0 11 165 10 734.8 0 10 166 7 734.8 0 7 167 11 734.8 0 11 168 16 734.8 0 16 169 14 734.8 0 14 170 11 734.8 0 11 171 13 734.8 0 13 172 11 734.8 0 11 173 10 734.8 0 10 174 21 734.8 0 21 175 42 734.8 0 42 176 115 734.8 0 115 177 113 734.8 0 113 178 57 734.8 0 57 179 10 734.8 0 10 180 7 734.8 0 7 181 9 734.8 0 9 182 12 734.8 0 12 183 12 734.8 0 12 184 9 734.8 0 9 185 7 734.8 0 7 186 10 734.8 0 10 187 6 734.8 0 6 188 18 734.8 0 18 189 12 734.8 0 12 190 7 734.8 0 7 191 13 734.8 0 13 192 3 734.8 0 3 193 24 734.8 0 24 194 56 734.8 0 56 195 40 734.8 0 40 196 15 734.8 0 15 197 17 734.8 0 17 198 17 734.8 0 17 199 12 734.8 0 12 200 16 734.8 0 16 201 26 734.8 0 26 202 28 734.8 0 28 203 10 734.8 0 10 204 13 734.8 0 13 205 20 734.8 0 20 206 22 734.8 0 22 207 16 734.8 0 16 208 24 734.8 0 24 209 20 734.8 0 20 210 13 734.8 0 13 211 13 734.8 0 13 212 15 734.8 0 15 213 29 734.8 0 29 214 33 734.8 0 33 215 24 734.8 0 24 216 10 734.8 0 10 217 37 734.8 0 37 218 11 734.8 0 11 219 13 734.8 0 13 220 17 734.8 0 17 221 13 734.8 0 13 222 16 734.8 0 16 223 11 734.8 0 11 224 16 734.8 0 16 225 13 734.8 0 13 226 15 734.8 0 15 227 17 734.8 0 17 228 14 734.8 0 14 229 12 734.8 0 12 230 23 734.8 0 23 231 17 734.8 0 17 232 18 734.8 0 18 233 14 734.8 0 14 234 149 734.8 0 149 235 82 734.8 0 82 236 24 734.8 0 24 237 18 734.8 0 18 238 14 734.8 0 14 239 37 734.8 0 37 240 30 734.8 0 30 241 28 734.8 0 28 242 21 734.8 0 21 243 12 734.8 0 12 244 25 734.8 0 25 245 16 734.8 0 16 246 19 734.8 0 19 247 19 734.8 0 19 248 18 734.8 0 18 249 26 734.8 0 26 250 13 734.8 0 13 251 15 734.8 0 15 252 10 734.8 0 10 253 34 734.8 0 34 254 24 734.8 0 24 255 45 734.8 0 45 256 36 734.8 0 36 257 26 734.8 0 26 258 48 734.8 0 48 259 355 734.8 0 355 260 949 734.8 0 949 261 876 734.8 0 876 262 71 734.8 0 71 263 10 734.8 0 10 264 11 734.8 0 11 265 29 734.8 0 29 266 16 734.8 0 16 267 10 734.8 0 10 268 27 734.8 0 27 269 6 734.8 0 6 270 12 734.8 0 12 271 6 734.8 0 6 272 9 734.8 0 9 273 21 734.8 0 21 274 6 734.8 0 6 275 12 734.8 0 12 276 18 734.8 0 18 277 9 734.8 0 9 278 12 734.8 0 12 279 139 734.8 0 139 280 121 734.8 0 121 281 20 734.8 0 20 282 8 734.8 0 8 283 8 734.8 0 8 284 15 734.8 0 15 285 6 734.8 0 6 286 12 734.8 0 12 287 12 734.8 0 12 288 17 734.8 0 17 289 8 734.8 0 8 290 9 734.8 0 9 291 19 734.8 0 19 292 21 734.8 0 21 293 28 734.8 0 28 294 23 734.8 0 23 295 21 734.8 0 21 296 20 734.8 0 20 297 29 734.8 0 29 298 47 734.8 0 47 299 80 734.8 0 80 300 44 734.8 0 44 301 27 734.8 0 27 Finished in 0.81 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 16,937 Reads with adapters: 6,913 (40.8%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 6,911 (40.8%) Total basepairs processed: 4,143,568 bp Total written (filtered): 1,888,070 bp (45.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6913 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 84 0.0 3 0 80 3 1 20 6472 0.0 4 6387 85 21 17 0.0 4 0 17 22 8 0.0 4 2 4 2 23 272 0.0 4 266 3 0 3 24 1 0.0 4 1 110 1 0.0 4 0 0 0 1 111 51 0.0 4 0 0 0 48 3 113 4 0.0 4 0 0 0 3 1 162 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 6,911 Reads with adapters: 690 (10.0%) Reads written (passing filters): 6,911 (100.0%) Total basepairs processed: 1,888,070 bp Total written (filtered): 1,720,136 bp (91.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 690 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 88.7% C: 2.3% G: 6.2% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 108.0 0 5 4 2 27.0 0 2 10 2 0.0 1 0 2 11 38 0.0 1 0 38 12 2 0.0 1 0 2 210 1 0.0 1 0 1 225 3 0.0 1 3 228 1 0.0 1 1 254 1 0.0 1 1 256 1 0.0 1 1 257 91 0.0 1 87 4 258 1 0.0 1 1 259 4 0.0 1 4 261 9 0.0 1 9 262 480 0.0 1 439 41 263 18 0.0 1 15 3 267 13 0.0 1 12 1 268 15 0.0 1 13 2 269 1 0.0 1 1 270 1 0.0 1 1 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.