This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.97 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 89,505 (3.0%) Reads that were too short: 48,343 (1.6%) Reads written (passing filters): 41,162 (1.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 6,756,896 bp (0.7%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 89505 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 214 734.8 0 214 7 1454 734.8 0 1454 8 24 734.8 0 24 9 1658 734.8 0 1658 10 11 734.8 0 11 11 7 734.8 0 7 12 29 734.8 0 29 13 4 734.8 0 4 14 7 734.8 0 7 15 11 734.8 0 11 16 5 734.8 0 5 17 10 734.8 0 10 18 3 734.8 0 3 19 7 734.8 0 7 20 6 734.8 0 6 21 20 734.8 0 20 22 24 734.8 0 24 23 16 734.8 0 16 24 10 734.8 0 10 25 4 734.8 0 4 26 10 734.8 0 10 27 6 734.8 0 6 28 4 734.8 0 4 29 9 734.8 0 9 30 9 734.8 0 9 31 14 734.8 0 14 32 12 734.8 0 12 33 6 734.8 0 6 34 18 734.8 0 18 35 9 734.8 0 9 36 9 734.8 0 9 37 5 734.8 0 5 38 17 734.8 0 17 39 10 734.8 0 10 40 19 734.8 0 19 41 141 734.8 0 141 42 64 734.8 0 64 43 5 734.8 0 5 44 14 734.8 0 14 45 7 734.8 0 7 46 9 734.8 0 9 47 9 734.8 0 9 48 12 734.8 0 12 49 11 734.8 0 11 50 9 734.8 0 9 51 17 734.8 0 17 52 19 734.8 0 19 53 11 734.8 0 11 54 33 734.8 0 33 55 14 734.8 0 14 56 9 734.8 0 9 57 10 734.8 0 10 58 7 734.8 0 7 59 11 734.8 0 11 60 16 734.8 0 16 61 10 734.8 0 10 62 7 734.8 0 7 63 16 734.8 0 16 64 8 734.8 0 8 65 15 734.8 0 15 66 9 734.8 0 9 67 12 734.8 0 12 68 11 734.8 0 11 69 122 734.8 0 122 70 217 734.8 0 217 71 108 734.8 0 108 72 12 734.8 0 12 73 17 734.8 0 17 74 16 734.8 0 16 75 36 734.8 0 36 76 35 734.8 0 35 77 23 734.8 0 23 78 34 734.8 0 34 79 20 734.8 0 20 80 99 734.8 0 99 81 106 734.8 0 106 82 98 734.8 0 98 83 55 734.8 0 55 84 100 734.8 0 100 85 119 734.8 0 119 86 99 734.8 0 99 87 131 734.8 0 131 88 230 734.8 0 230 89 254 734.8 0 254 90 158 734.8 0 158 91 174 734.8 0 174 92 53 734.8 0 53 93 110 734.8 0 110 94 149 734.8 0 149 95 87 734.8 0 87 96 73 734.8 0 73 97 91 734.8 0 91 98 67 734.8 0 67 99 79 734.8 0 79 100 648 734.8 0 648 101 713 734.8 0 713 102 661 734.8 0 661 103 273 734.8 0 273 104 162 734.8 0 162 105 97 734.8 0 97 106 46 734.8 0 46 107 54 734.8 0 54 108 26 734.8 0 26 109 38 734.8 0 38 110 75 734.8 0 75 111 97 734.8 0 97 112 112 734.8 0 112 113 84 734.8 0 84 114 107 734.8 0 107 115 134 734.8 0 134 116 158 734.8 0 158 117 219 734.8 0 219 118 280 734.8 0 280 119 179 734.8 0 179 120 121 734.8 0 121 121 127 734.8 0 127 122 148 734.8 0 148 123 381 734.8 0 381 124 422 734.8 0 422 125 192 734.8 0 192 126 145 734.8 0 145 127 134 734.8 0 134 128 192 734.8 0 192 129 116 734.8 0 116 130 178 734.8 0 178 131 353 734.8 0 353 132 575 734.8 0 575 133 484 734.8 0 484 134 218 734.8 0 218 135 169 734.8 0 169 136 190 734.8 0 190 137 295 734.8 0 295 138 280 734.8 0 280 139 244 734.8 0 244 140 228 734.8 0 228 141 232 734.8 0 232 142 574 734.8 0 574 143 1106 734.8 0 1106 144 679 734.8 0 679 145 296 734.8 0 296 146 242 734.8 0 242 147 243 734.8 0 243 148 317 734.8 0 317 149 344 734.8 0 344 150 304 734.8 0 304 151 373 734.8 0 373 152 275 734.8 0 275 153 268 734.8 0 268 154 272 734.8 0 272 155 261 734.8 0 261 156 334 734.8 0 334 157 454 734.8 0 454 158 572 734.8 0 572 159 580 734.8 0 580 160 732 734.8 0 732 161 567 734.8 0 567 162 509 734.8 0 509 163 376 734.8 0 376 164 314 734.8 0 314 165 241 734.8 0 241 166 181 734.8 0 181 167 276 734.8 0 276 168 499 734.8 0 499 169 529 734.8 0 529 170 476 734.8 0 476 171 373 734.8 0 373 172 334 734.8 0 334 173 235 734.8 0 235 174 226 734.8 0 226 175 199 734.8 0 199 176 746 734.8 0 746 177 857 734.8 0 857 178 523 734.8 0 523 179 401 734.8 0 401 180 320 734.8 0 320 181 261 734.8 0 261 182 357 734.8 0 357 183 399 734.8 0 399 184 467 734.8 0 467 185 368 734.8 0 368 186 355 734.8 0 355 187 488 734.8 0 488 188 588 734.8 0 588 189 745 734.8 0 745 190 402 734.8 0 402 191 232 734.8 0 232 192 204 734.8 0 204 193 199 734.8 0 199 194 337 734.8 0 337 195 304 734.8 0 304 196 534 734.8 0 534 197 378 734.8 0 378 198 297 734.8 0 297 199 383 734.8 0 383 200 344 734.8 0 344 201 356 734.8 0 356 202 320 734.8 0 320 203 228 734.8 0 228 204 312 734.8 0 312 205 322 734.8 0 322 206 342 734.8 0 342 207 310 734.8 0 310 208 279 734.8 0 279 209 318 734.8 0 318 210 430 734.8 0 430 211 590 734.8 0 590 212 449 734.8 0 449 213 458 734.8 0 458 214 570 734.8 0 570 215 572 734.8 0 572 216 480 734.8 0 480 217 464 734.8 0 464 218 447 734.8 0 447 219 422 734.8 0 422 220 469 734.8 0 469 221 402 734.8 0 402 222 670 734.8 0 670 223 670 734.8 0 670 224 708 734.8 0 708 225 604 734.8 0 604 226 449 734.8 0 449 227 465 734.8 0 465 228 443 734.8 0 443 229 404 734.8 0 404 230 466 734.8 0 466 231 285 734.8 0 285 232 298 734.8 0 298 233 353 734.8 0 353 234 404 734.8 0 404 235 364 734.8 0 364 236 459 734.8 0 459 237 461 734.8 0 461 238 601 734.8 0 601 239 1243 734.8 0 1243 240 603 734.8 0 603 241 479 734.8 0 479 242 466 734.8 0 466 243 484 734.8 0 484 244 514 734.8 0 514 245 660 734.8 0 660 246 580 734.8 0 580 247 674 734.8 0 674 248 568 734.8 0 568 249 691 734.8 0 691 250 391 734.8 0 391 251 387 734.8 0 387 252 236 734.8 0 236 253 397 734.8 0 397 254 538 734.8 0 538 255 657 734.8 0 657 256 618 734.8 0 618 257 474 734.8 0 474 258 459 734.8 0 459 259 469 734.8 0 469 260 642 734.8 0 642 261 335 734.8 0 335 262 337 734.8 0 337 263 513 734.8 0 513 264 731 734.8 0 731 265 641 734.8 0 641 266 440 734.8 0 440 267 447 734.8 0 447 268 408 734.8 0 408 269 620 734.8 0 620 270 472 734.8 0 472 271 525 734.8 0 525 272 383 734.8 0 383 273 421 734.8 0 421 274 533 734.8 0 533 275 622 734.8 0 622 276 706 734.8 0 706 277 593 734.8 0 593 278 335 734.8 0 335 279 274 734.8 0 274 280 467 734.8 0 467 281 570 734.8 0 570 282 659 734.8 0 659 283 716 734.8 0 716 284 483 734.8 0 483 285 470 734.8 0 470 286 470 734.8 0 470 287 492 734.8 0 492 288 588 734.8 0 588 289 440 734.8 0 440 290 302 734.8 0 302 291 315 734.8 0 315 292 360 734.8 0 360 293 446 734.8 0 446 294 540 734.8 0 540 295 458 734.8 0 458 296 391 734.8 0 391 297 314 734.8 0 314 298 420 734.8 0 420 299 436 734.8 0 436 300 451 734.8 0 451 301 701 734.8 0 701 Finished in 1.33 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 41,162 Reads with adapters: 1,897 (4.6%) Reads that were too short: 32 (0.1%) Reads written (passing filters): 1,865 (4.5%) Total basepairs processed: 6,756,896 bp Total written (filtered): 500,218 bp (7.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1897 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 16 0.0 3 0 16 17 1638 0.0 3 1599 33 3 3 18 4 0.0 3 0 4 19 4 0.0 3 0 1 0 3 20 118 0.0 3 115 1 1 1 21 1 0.0 3 0 1 22 4 0.0 3 0 0 0 4 28 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 49 17 0.0 3 0 0 0 17 52 7 0.0 3 0 0 0 7 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 13 0.0 3 0 0 0 13 72 4 0.0 3 0 0 0 4 73 6 0.0 3 0 0 0 6 74 10 0.0 3 0 0 1 9 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 1 149 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 1 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 1,865 Reads with adapters: 128 (6.9%) Reads written (passing filters): 1,865 (100.0%) Total basepairs processed: 500,218 bp Total written (filtered): 499,830 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 128 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.3% C: 6.2% G: 24.2% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 124 29.1 0 124 4 4 7.3 0 4