This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.05 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 31,199 (1.0%) Reads that were too short: 13,556 (0.5%) Reads written (passing filters): 17,643 (0.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 3,233,521 bp (0.4%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 31199 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 18 734.8 0 18 7 19 734.8 0 19 8 91 734.8 0 91 9 1759 734.8 0 1759 10 18 734.8 0 18 11 4 734.8 0 4 12 6 734.8 0 6 13 8 734.8 0 8 14 5 734.8 0 5 15 4 734.8 0 4 16 6 734.8 0 6 17 12 734.8 0 12 18 25 734.8 0 25 19 11 734.8 0 11 20 15 734.8 0 15 21 6 734.8 0 6 22 8 734.8 0 8 23 15 734.8 0 15 24 12 734.8 0 12 25 12 734.8 0 12 26 8 734.8 0 8 27 6 734.8 0 6 28 25 734.8 0 25 29 23 734.8 0 23 30 17 734.8 0 17 31 23 734.8 0 23 32 12 734.8 0 12 33 17 734.8 0 17 34 13 734.8 0 13 35 18 734.8 0 18 36 15 734.8 0 15 37 11 734.8 0 11 38 9 734.8 0 9 39 11 734.8 0 11 40 10 734.8 0 10 41 8 734.8 0 8 42 7 734.8 0 7 43 15 734.8 0 15 44 14 734.8 0 14 45 176 734.8 0 176 46 144 734.8 0 144 47 30 734.8 0 30 48 12 734.8 0 12 49 6 734.8 0 6 50 14 734.8 0 14 51 14 734.8 0 14 52 6 734.8 0 6 53 17 734.8 0 17 54 9 734.8 0 9 55 7 734.8 0 7 56 14 734.8 0 14 57 8 734.8 0 8 58 11 734.8 0 11 59 9 734.8 0 9 60 8 734.8 0 8 61 6 734.8 0 6 62 11 734.8 0 11 63 4 734.8 0 4 64 9 734.8 0 9 65 7 734.8 0 7 66 5 734.8 0 5 67 9 734.8 0 9 68 7 734.8 0 7 69 34 734.8 0 34 70 17 734.8 0 17 71 52 734.8 0 52 72 25 734.8 0 25 73 8 734.8 0 8 74 12 734.8 0 12 75 12 734.8 0 12 76 22 734.8 0 22 77 119 734.8 0 119 78 36 734.8 0 36 79 47 734.8 0 47 80 52 734.8 0 52 81 41 734.8 0 41 82 32 734.8 0 32 83 38 734.8 0 38 84 48 734.8 0 48 85 17 734.8 0 17 86 7 734.8 0 7 87 6 734.8 0 6 88 15 734.8 0 15 89 36 734.8 0 36 90 72 734.8 0 72 91 317 734.8 0 317 92 828 734.8 0 828 93 230 734.8 0 230 94 117 734.8 0 117 95 371 734.8 0 371 96 347 734.8 0 347 97 178 734.8 0 178 98 134 734.8 0 134 99 84 734.8 0 84 100 158 734.8 0 158 101 60 734.8 0 60 102 19 734.8 0 19 103 17 734.8 0 17 104 7 734.8 0 7 105 17 734.8 0 17 106 29 734.8 0 29 107 43 734.8 0 43 108 79 734.8 0 79 109 59 734.8 0 59 110 71 734.8 0 71 111 44 734.8 0 44 112 67 734.8 0 67 113 325 734.8 0 325 114 588 734.8 0 588 115 351 734.8 0 351 116 155 734.8 0 155 117 93 734.8 0 93 118 64 734.8 0 64 119 67 734.8 0 67 120 84 734.8 0 84 121 76 734.8 0 76 122 95 734.8 0 95 123 105 734.8 0 105 124 69 734.8 0 69 125 82 734.8 0 82 126 95 734.8 0 95 127 66 734.8 0 66 128 121 734.8 0 121 129 167 734.8 0 167 130 144 734.8 0 144 131 139 734.8 0 139 132 111 734.8 0 111 133 92 734.8 0 92 134 103 734.8 0 103 135 224 734.8 0 224 136 341 734.8 0 341 137 179 734.8 0 179 138 256 734.8 0 256 139 120 734.8 0 120 140 127 734.8 0 127 141 76 734.8 0 76 142 102 734.8 0 102 143 104 734.8 0 104 144 107 734.8 0 107 145 90 734.8 0 90 146 58 734.8 0 58 147 77 734.8 0 77 148 80 734.8 0 80 149 76 734.8 0 76 150 70 734.8 0 70 151 63 734.8 0 63 152 75 734.8 0 75 153 112 734.8 0 112 154 126 734.8 0 126 155 114 734.8 0 114 156 108 734.8 0 108 157 102 734.8 0 102 158 99 734.8 0 99 159 67 734.8 0 67 160 53 734.8 0 53 161 71 734.8 0 71 162 87 734.8 0 87 163 158 734.8 0 158 164 130 734.8 0 130 165 119 734.8 0 119 166 70 734.8 0 70 167 128 734.8 0 128 168 181 734.8 0 181 169 163 734.8 0 163 170 95 734.8 0 95 171 97 734.8 0 97 172 82 734.8 0 82 173 104 734.8 0 104 174 97 734.8 0 97 175 87 734.8 0 87 176 54 734.8 0 54 177 120 734.8 0 120 178 122 734.8 0 122 179 101 734.8 0 101 180 124 734.8 0 124 181 97 734.8 0 97 182 110 734.8 0 110 183 128 734.8 0 128 184 145 734.8 0 145 185 156 734.8 0 156 186 98 734.8 0 98 187 82 734.8 0 82 188 120 734.8 0 120 189 128 734.8 0 128 190 180 734.8 0 180 191 197 734.8 0 197 192 111 734.8 0 111 193 85 734.8 0 85 194 86 734.8 0 86 195 109 734.8 0 109 196 112 734.8 0 112 197 143 734.8 0 143 198 77 734.8 0 77 199 55 734.8 0 55 200 81 734.8 0 81 201 72 734.8 0 72 202 159 734.8 0 159 203 298 734.8 0 298 204 212 734.8 0 212 205 140 734.8 0 140 206 82 734.8 0 82 207 62 734.8 0 62 208 124 734.8 0 124 209 153 734.8 0 153 210 224 734.8 0 224 211 152 734.8 0 152 212 111 734.8 0 111 213 98 734.8 0 98 214 118 734.8 0 118 215 164 734.8 0 164 216 179 734.8 0 179 217 143 734.8 0 143 218 93 734.8 0 93 219 76 734.8 0 76 220 86 734.8 0 86 221 95 734.8 0 95 222 90 734.8 0 90 223 135 734.8 0 135 224 172 734.8 0 172 225 674 734.8 0 674 226 777 734.8 0 777 227 383 734.8 0 383 228 108 734.8 0 108 229 61 734.8 0 61 230 77 734.8 0 77 231 78 734.8 0 78 232 125 734.8 0 125 233 122 734.8 0 122 234 197 734.8 0 197 235 187 734.8 0 187 236 215 734.8 0 215 237 162 734.8 0 162 238 64 734.8 0 64 239 65 734.8 0 65 240 83 734.8 0 83 241 55 734.8 0 55 242 83 734.8 0 83 243 129 734.8 0 129 244 83 734.8 0 83 245 96 734.8 0 96 246 61 734.8 0 61 247 32 734.8 0 32 248 31 734.8 0 31 249 36 734.8 0 36 250 62 734.8 0 62 251 98 734.8 0 98 252 410 734.8 0 410 253 133 734.8 0 133 254 79 734.8 0 79 255 60 734.8 0 60 256 47 734.8 0 47 257 41 734.8 0 41 258 50 734.8 0 50 259 79 734.8 0 79 260 89 734.8 0 89 261 96 734.8 0 96 262 79 734.8 0 79 263 94 734.8 0 94 264 83 734.8 0 83 265 83 734.8 0 83 266 46 734.8 0 46 267 34 734.8 0 34 268 48 734.8 0 48 269 76 734.8 0 76 270 168 734.8 0 168 271 122 734.8 0 122 272 95 734.8 0 95 273 71 734.8 0 71 274 66 734.8 0 66 275 63 734.8 0 63 276 70 734.8 0 70 277 57 734.8 0 57 278 83 734.8 0 83 279 68 734.8 0 68 280 187 734.8 0 187 281 396 734.8 0 396 282 194 734.8 0 194 283 232 734.8 0 232 284 113 734.8 0 113 285 110 734.8 0 110 286 96 734.8 0 96 287 125 734.8 0 125 288 78 734.8 0 78 289 104 734.8 0 104 290 335 734.8 0 335 291 185 734.8 0 185 292 119 734.8 0 119 293 54 734.8 0 54 294 64 734.8 0 64 295 221 734.8 0 221 296 354 734.8 0 354 297 277 734.8 0 277 298 180 734.8 0 180 299 164 734.8 0 164 300 109 734.8 0 109 301 59 734.8 0 59 Finished in 0.68 s (39 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 17,643 Reads with adapters: 1,805 (10.2%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 1,802 (10.2%) Total basepairs processed: 3,233,521 bp Total written (filtered): 484,239 bp (15.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1805 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 22 0.0 3 0 22 20 1711 0.0 4 1677 32 0 0 2 21 7 0.0 4 1 6 29 2 0.0 4 0 0 0 1 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 69 3 0.0 4 0 0 0 0 3 89 6 0.0 4 0 0 0 0 6 103 1 0.0 4 0 0 0 1 110 1 0.0 4 0 0 0 1 111 47 0.0 4 0 0 0 42 5 144 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 1,802 Reads with adapters: 153 (8.5%) Reads written (passing filters): 1,802 (100.0%) Total basepairs processed: 484,239 bp Total written (filtered): 460,500 bp (95.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 153 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 58.2% C: 0.7% G: 40.5% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 12 0.0 0 0 12 10 37 0.0 1 2 35 11 14 0.0 1 0 14 223 1 0.0 1 1 257 87 0.0 1 82 5 258 1 0.0 1 1 267 1 0.0 1 1