This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.58 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 94,525 (3.1%) Reads that were too short: 62,078 (2.1%) Reads written (passing filters): 32,447 (1.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 8,015,784 bp (0.9%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 94525 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1197 734.8 0 1197 7 253 734.8 0 253 8 15394 734.8 0 15394 9 2381 734.8 0 2381 10 31 734.8 0 31 11 39 734.8 0 39 12 65 734.8 0 65 13 100 734.8 0 100 14 31 734.8 0 31 15 31 734.8 0 31 16 27 734.8 0 27 17 52 734.8 0 52 18 31 734.8 0 31 19 30 734.8 0 30 20 27 734.8 0 27 21 43 734.8 0 43 22 30 734.8 0 30 23 40 734.8 0 40 24 34 734.8 0 34 25 40 734.8 0 40 26 22 734.8 0 22 27 27 734.8 0 27 28 31 734.8 0 31 29 39 734.8 0 39 30 27 734.8 0 27 31 31 734.8 0 31 32 45 734.8 0 45 33 23 734.8 0 23 34 26 734.8 0 26 35 34 734.8 0 34 36 54 734.8 0 54 37 42 734.8 0 42 38 30 734.8 0 30 39 47 734.8 0 47 40 325 734.8 0 325 41 33 734.8 0 33 42 39 734.8 0 39 43 42 734.8 0 42 44 30 734.8 0 30 45 35 734.8 0 35 46 40 734.8 0 40 47 36 734.8 0 36 48 38 734.8 0 38 49 42 734.8 0 42 50 33 734.8 0 33 51 75 734.8 0 75 52 57 734.8 0 57 53 63 734.8 0 63 54 32 734.8 0 32 55 32 734.8 0 32 56 24 734.8 0 24 57 19 734.8 0 19 58 41 734.8 0 41 59 22 734.8 0 22 60 29 734.8 0 29 61 31 734.8 0 31 62 80 734.8 0 80 63 51 734.8 0 51 64 37 734.8 0 37 65 35 734.8 0 35 66 31 734.8 0 31 67 31 734.8 0 31 68 44 734.8 0 44 69 60 734.8 0 60 70 28 734.8 0 28 71 44 734.8 0 44 72 39 734.8 0 39 73 34 734.8 0 34 74 55 734.8 0 55 75 43 734.8 0 43 76 36 734.8 0 36 77 37 734.8 0 37 78 43 734.8 0 43 79 51 734.8 0 51 80 54 734.8 0 54 81 52 734.8 0 52 82 65 734.8 0 65 83 83 734.8 0 83 84 54 734.8 0 54 85 74 734.8 0 74 86 139 734.8 0 139 87 143 734.8 0 143 88 66 734.8 0 66 89 57 734.8 0 57 90 53 734.8 0 53 91 46 734.8 0 46 92 71 734.8 0 71 93 102 734.8 0 102 94 83 734.8 0 83 95 82 734.8 0 82 96 78 734.8 0 78 97 72 734.8 0 72 98 54 734.8 0 54 99 70 734.8 0 70 100 84 734.8 0 84 101 104 734.8 0 104 102 72 734.8 0 72 103 54 734.8 0 54 104 38 734.8 0 38 105 43 734.8 0 43 106 37 734.8 0 37 107 36 734.8 0 36 108 27 734.8 0 27 109 42 734.8 0 42 110 34 734.8 0 34 111 45 734.8 0 45 112 45 734.8 0 45 113 49 734.8 0 49 114 40 734.8 0 40 115 47 734.8 0 47 116 120 734.8 0 120 117 150 734.8 0 150 118 116 734.8 0 116 119 91 734.8 0 91 120 65 734.8 0 65 121 62 734.8 0 62 122 60 734.8 0 60 123 68 734.8 0 68 124 49 734.8 0 49 125 81 734.8 0 81 126 85 734.8 0 85 127 69 734.8 0 69 128 43 734.8 0 43 129 53 734.8 0 53 130 57 734.8 0 57 131 87 734.8 0 87 132 93 734.8 0 93 133 103 734.8 0 103 134 76 734.8 0 76 135 63 734.8 0 63 136 96 734.8 0 96 137 142 734.8 0 142 138 474 734.8 0 474 139 418 734.8 0 418 140 153 734.8 0 153 141 73 734.8 0 73 142 91 734.8 0 91 143 79 734.8 0 79 144 86 734.8 0 86 145 96 734.8 0 96 146 86 734.8 0 86 147 65 734.8 0 65 148 62 734.8 0 62 149 84 734.8 0 84 150 82 734.8 0 82 151 80 734.8 0 80 152 60 734.8 0 60 153 58 734.8 0 58 154 41 734.8 0 41 155 73 734.8 0 73 156 78 734.8 0 78 157 83 734.8 0 83 158 89 734.8 0 89 159 96 734.8 0 96 160 74 734.8 0 74 161 69 734.8 0 69 162 69 734.8 0 69 163 43 734.8 0 43 164 41 734.8 0 41 165 71 734.8 0 71 166 61 734.8 0 61 167 70 734.8 0 70 168 65 734.8 0 65 169 82 734.8 0 82 170 84 734.8 0 84 171 126 734.8 0 126 172 72 734.8 0 72 173 59 734.8 0 59 174 66 734.8 0 66 175 62 734.8 0 62 176 78 734.8 0 78 177 78 734.8 0 78 178 70 734.8 0 70 179 100 734.8 0 100 180 104 734.8 0 104 181 84 734.8 0 84 182 83 734.8 0 83 183 83 734.8 0 83 184 88 734.8 0 88 185 66 734.8 0 66 186 102 734.8 0 102 187 236 734.8 0 236 188 248 734.8 0 248 189 72 734.8 0 72 190 54 734.8 0 54 191 55 734.8 0 55 192 68 734.8 0 68 193 72 734.8 0 72 194 107 734.8 0 107 195 118 734.8 0 118 196 65 734.8 0 65 197 68 734.8 0 68 198 73 734.8 0 73 199 108 734.8 0 108 200 95 734.8 0 95 201 76 734.8 0 76 202 43 734.8 0 43 203 56 734.8 0 56 204 93 734.8 0 93 205 91 734.8 0 91 206 101 734.8 0 101 207 100 734.8 0 100 208 69 734.8 0 69 209 87 734.8 0 87 210 87 734.8 0 87 211 74 734.8 0 74 212 85 734.8 0 85 213 99 734.8 0 99 214 210 734.8 0 210 215 164 734.8 0 164 216 118 734.8 0 118 217 87 734.8 0 87 218 84 734.8 0 84 219 99 734.8 0 99 220 92 734.8 0 92 221 84 734.8 0 84 222 54 734.8 0 54 223 42 734.8 0 42 224 82 734.8 0 82 225 71 734.8 0 71 226 81 734.8 0 81 227 101 734.8 0 101 228 111 734.8 0 111 229 87 734.8 0 87 230 82 734.8 0 82 231 62 734.8 0 62 232 57 734.8 0 57 233 52 734.8 0 52 234 54 734.8 0 54 235 46 734.8 0 46 236 92 734.8 0 92 237 112 734.8 0 112 238 88 734.8 0 88 239 105 734.8 0 105 240 88 734.8 0 88 241 49 734.8 0 49 242 40 734.8 0 40 243 54 734.8 0 54 244 77 734.8 0 77 245 70 734.8 0 70 246 67 734.8 0 67 247 67 734.8 0 67 248 105 734.8 0 105 249 97 734.8 0 97 250 53 734.8 0 53 251 50 734.8 0 50 252 41 734.8 0 41 253 43 734.8 0 43 254 58 734.8 0 58 255 72 734.8 0 72 256 83 734.8 0 83 257 105 734.8 0 105 258 147 734.8 0 147 259 168 734.8 0 168 260 157 734.8 0 157 261 62 734.8 0 62 262 73 734.8 0 73 263 68 734.8 0 68 264 65 734.8 0 65 265 55 734.8 0 55 266 85 734.8 0 85 267 91 734.8 0 91 268 123 734.8 0 123 269 124 734.8 0 124 270 87 734.8 0 87 271 90 734.8 0 90 272 156 734.8 0 156 273 1148 734.8 0 1148 274 5255 734.8 0 5255 275 982 734.8 0 982 276 262 734.8 0 262 277 117 734.8 0 117 278 79 734.8 0 79 279 66 734.8 0 66 280 157 734.8 0 157 281 176 734.8 0 176 282 143 734.8 0 143 283 157 734.8 0 157 284 368 734.8 0 368 285 233 734.8 0 233 286 384 734.8 0 384 287 310 734.8 0 310 288 297 734.8 0 297 289 217 734.8 0 217 290 168 734.8 0 168 291 111 734.8 0 111 292 201 734.8 0 201 293 1479 734.8 0 1479 294 2316 734.8 0 2316 295 1184 734.8 0 1184 296 788 734.8 0 788 297 1398 734.8 0 1398 298 2782 734.8 0 2782 299 11640 734.8 0 11640 300 16793 734.8 0 16793 301 6795 734.8 0 6795 Finished in 1.39 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 32,447 Reads with adapters: 15,760 (48.6%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 15,754 (48.6%) Total basepairs processed: 8,015,784 bp Total written (filtered): 4,345,993 bp (54.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15760 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 9 0.0 3 0 0 6 3 16 113 0.0 3 0 104 6 3 17 15326 0.0 3 14841 431 39 15 18 45 0.0 3 1 41 2 1 19 133 0.0 3 99 4 15 15 20 107 0.0 3 82 1 2 22 21 2 0.0 3 0 1 0 1 28 1 0.0 3 1 29 2 0.0 3 0 0 1 1 32 1 0.0 3 0 0 0 1 35 1 0.0 3 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 1 43 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 15,754 Reads with adapters: 379 (2.4%) Reads written (passing filters): 15,754 (100.0%) Total basepairs processed: 4,345,993 bp Total written (filtered): 4,344,844 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 379 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.9% C: 8.4% G: 28.2% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 368 246.2 0 368 4 10 61.5 0 10 5 1 15.4 0 1