This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.10 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 515,138 (17.1%) Reads that were too short: 65,772 (2.2%) Reads written (passing filters): 449,366 (14.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 74,437,463 bp (8.2%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 515138 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 135 734.8 0 135 7 669 734.8 0 669 8 15768 734.8 0 15768 9 168 734.8 0 168 10 95 734.8 0 95 11 75 734.8 0 75 12 73 734.8 0 73 13 65 734.8 0 65 14 68 734.8 0 68 15 75 734.8 0 75 16 82 734.8 0 82 17 91 734.8 0 91 18 47 734.8 0 47 19 39 734.8 0 39 20 34 734.8 0 34 21 45 734.8 0 45 22 74 734.8 0 74 23 87 734.8 0 87 24 57 734.8 0 57 25 96 734.8 0 96 26 104 734.8 0 104 27 58 734.8 0 58 28 131 734.8 0 131 29 105 734.8 0 105 30 83 734.8 0 83 31 46 734.8 0 46 32 32 734.8 0 32 33 54 734.8 0 54 34 58 734.8 0 58 35 72 734.8 0 72 36 59 734.8 0 59 37 61 734.8 0 61 38 152 734.8 0 152 39 96 734.8 0 96 40 49 734.8 0 49 41 71 734.8 0 71 42 62 734.8 0 62 43 57 734.8 0 57 44 58 734.8 0 58 45 56 734.8 0 56 46 54 734.8 0 54 47 38 734.8 0 38 48 43 734.8 0 43 49 57 734.8 0 57 50 82 734.8 0 82 51 56 734.8 0 56 52 51 734.8 0 51 53 96 734.8 0 96 54 84 734.8 0 84 55 65 734.8 0 65 56 96 734.8 0 96 57 60 734.8 0 60 58 56 734.8 0 56 59 46 734.8 0 46 60 77 734.8 0 77 61 65 734.8 0 65 62 75 734.8 0 75 63 78 734.8 0 78 64 67 734.8 0 67 65 59 734.8 0 59 66 93 734.8 0 93 67 349 734.8 0 349 68 121 734.8 0 121 69 174 734.8 0 174 70 164 734.8 0 164 71 88 734.8 0 88 72 99 734.8 0 99 73 122 734.8 0 122 74 67 734.8 0 67 75 100 734.8 0 100 76 115 734.8 0 115 77 118 734.8 0 118 78 289 734.8 0 289 79 592 734.8 0 592 80 649 734.8 0 649 81 1680 734.8 0 1680 82 1619 734.8 0 1619 83 1385 734.8 0 1385 84 2410 734.8 0 2410 85 3529 734.8 0 3529 86 1206 734.8 0 1206 87 362 734.8 0 362 88 410 734.8 0 410 89 320 734.8 0 320 90 420 734.8 0 420 91 256 734.8 0 256 92 605 734.8 0 605 93 873 734.8 0 873 94 552 734.8 0 552 95 1208 734.8 0 1208 96 853 734.8 0 853 97 585 734.8 0 585 98 1613 734.8 0 1613 99 997 734.8 0 997 100 552 734.8 0 552 101 1127 734.8 0 1127 102 882 734.8 0 882 103 502 734.8 0 502 104 641 734.8 0 641 105 507 734.8 0 507 106 404 734.8 0 404 107 417 734.8 0 417 108 521 734.8 0 521 109 556 734.8 0 556 110 814 734.8 0 814 111 975 734.8 0 975 112 697 734.8 0 697 113 1074 734.8 0 1074 114 1134 734.8 0 1134 115 1351 734.8 0 1351 116 1282 734.8 0 1282 117 1975 734.8 0 1975 118 16575 734.8 0 16575 119 20074 734.8 0 20074 120 5084 734.8 0 5084 121 2508 734.8 0 2508 122 3472 734.8 0 3472 123 2492 734.8 0 2492 124 930 734.8 0 930 125 935 734.8 0 935 126 1299 734.8 0 1299 127 1154 734.8 0 1154 128 865 734.8 0 865 129 1347 734.8 0 1347 130 1112 734.8 0 1112 131 1133 734.8 0 1133 132 1829 734.8 0 1829 133 1364 734.8 0 1364 134 1646 734.8 0 1646 135 1607 734.8 0 1607 136 1648 734.8 0 1648 137 1877 734.8 0 1877 138 1942 734.8 0 1942 139 6126 734.8 0 6126 140 24436 734.8 0 24436 141 17674 734.8 0 17674 142 3675 734.8 0 3675 143 2913 734.8 0 2913 144 4374 734.8 0 4374 145 70353 734.8 0 70353 146 52901 734.8 0 52901 147 33943 734.8 0 33943 148 26542 734.8 0 26542 149 7394 734.8 0 7394 150 2072 734.8 0 2072 151 1293 734.8 0 1293 152 1071 734.8 0 1071 153 1301 734.8 0 1301 154 968 734.8 0 968 155 1177 734.8 0 1177 156 1120 734.8 0 1120 157 1085 734.8 0 1085 158 1413 734.8 0 1413 159 929 734.8 0 929 160 914 734.8 0 914 161 870 734.8 0 870 162 979 734.8 0 979 163 1068 734.8 0 1068 164 1158 734.8 0 1158 165 4554 734.8 0 4554 166 9808 734.8 0 9808 167 2147 734.8 0 2147 168 1086 734.8 0 1086 169 1014 734.8 0 1014 170 1018 734.8 0 1018 171 954 734.8 0 954 172 1005 734.8 0 1005 173 1181 734.8 0 1181 174 980 734.8 0 980 175 1016 734.8 0 1016 176 1100 734.8 0 1100 177 799 734.8 0 799 178 1081 734.8 0 1081 179 778 734.8 0 778 180 762 734.8 0 762 181 1036 734.8 0 1036 182 872 734.8 0 872 183 916 734.8 0 916 184 1051 734.8 0 1051 185 1159 734.8 0 1159 186 973 734.8 0 973 187 805 734.8 0 805 188 995 734.8 0 995 189 886 734.8 0 886 190 881 734.8 0 881 191 895 734.8 0 895 192 1117 734.8 0 1117 193 1363 734.8 0 1363 194 974 734.8 0 974 195 1255 734.8 0 1255 196 1208 734.8 0 1208 197 799 734.8 0 799 198 1168 734.8 0 1168 199 936 734.8 0 936 200 1023 734.8 0 1023 201 1083 734.8 0 1083 202 645 734.8 0 645 203 795 734.8 0 795 204 851 734.8 0 851 205 910 734.8 0 910 206 834 734.8 0 834 207 718 734.8 0 718 208 725 734.8 0 725 209 911 734.8 0 911 210 799 734.8 0 799 211 721 734.8 0 721 212 760 734.8 0 760 213 728 734.8 0 728 214 704 734.8 0 704 215 685 734.8 0 685 216 825 734.8 0 825 217 1392 734.8 0 1392 218 1105 734.8 0 1105 219 942 734.8 0 942 220 719 734.8 0 719 221 793 734.8 0 793 222 622 734.8 0 622 223 574 734.8 0 574 224 545 734.8 0 545 225 612 734.8 0 612 226 634 734.8 0 634 227 580 734.8 0 580 228 500 734.8 0 500 229 571 734.8 0 571 230 656 734.8 0 656 231 652 734.8 0 652 232 597 734.8 0 597 233 605 734.8 0 605 234 624 734.8 0 624 235 625 734.8 0 625 236 635 734.8 0 635 237 803 734.8 0 803 238 714 734.8 0 714 239 700 734.8 0 700 240 768 734.8 0 768 241 699 734.8 0 699 242 557 734.8 0 557 243 503 734.8 0 503 244 562 734.8 0 562 245 534 734.8 0 534 246 638 734.8 0 638 247 1340 734.8 0 1340 248 746 734.8 0 746 249 591 734.8 0 591 250 683 734.8 0 683 251 560 734.8 0 560 252 386 734.8 0 386 253 541 734.8 0 541 254 439 734.8 0 439 255 434 734.8 0 434 256 421 734.8 0 421 257 483 734.8 0 483 258 460 734.8 0 460 259 495 734.8 0 495 260 642 734.8 0 642 261 578 734.8 0 578 262 497 734.8 0 497 263 504 734.8 0 504 264 459 734.8 0 459 265 441 734.8 0 441 266 543 734.8 0 543 267 462 734.8 0 462 268 604 734.8 0 604 269 547 734.8 0 547 270 630 734.8 0 630 271 600 734.8 0 600 272 486 734.8 0 486 273 574 734.8 0 574 274 555 734.8 0 555 275 438 734.8 0 438 276 421 734.8 0 421 277 398 734.8 0 398 278 430 734.8 0 430 279 498 734.8 0 498 280 359 734.8 0 359 281 503 734.8 0 503 282 487 734.8 0 487 283 301 734.8 0 301 284 447 734.8 0 447 285 493 734.8 0 493 286 408 734.8 0 408 287 501 734.8 0 501 288 781 734.8 0 781 289 777 734.8 0 777 290 747 734.8 0 747 291 784 734.8 0 784 292 774 734.8 0 774 293 685 734.8 0 685 294 861 734.8 0 861 295 964 734.8 0 964 296 902 734.8 0 902 297 1200 734.8 0 1200 298 1075 734.8 0 1075 299 810 734.8 0 810 300 610 734.8 0 610 301 1345 734.8 0 1345 Finished in 15.67 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 449,366 Reads with adapters: 16,186 (3.6%) Reads that were too short: 74 (0.0%) Reads written (passing filters): 16,112 (3.6%) Total basepairs processed: 74,437,463 bp Total written (filtered): 4,378,929 bp (5.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16186 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 2 4 18 9 0.0 3 0 0 8 0 1 19 184 0.0 3 0 180 1 1 2 20 15658 0.0 4 15475 170 8 4 1 21 55 0.0 4 7 47 0 0 1 22 10 0.0 4 2 0 5 0 3 23 11 0.0 4 0 0 0 10 1 24 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 3 0.0 4 0 0 0 0 3 41 4 0.0 4 0 0 0 0 4 42 3 0.0 4 0 0 0 0 3 44 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 50 151 0.0 4 0 0 0 0 151 53 8 0.0 4 0 0 0 0 8 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 3 0.0 4 0 0 0 0 3 111 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 4 0.0 4 0 0 0 0 4 125 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 136 3 0.0 4 0 0 0 0 3 137 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 148 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 155 3 0.0 4 0 0 0 0 3 157 1 0.0 4 0 0 0 0 1 158 3 0.0 4 0 0 0 0 3 168 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.54 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 16,112 Reads with adapters: 865 (5.4%) Reads written (passing filters): 16,112 (100.0%) Total basepairs processed: 4,378,929 bp Total written (filtered): 4,249,473 bp (97.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 865 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 0.9% G: 44.7% T: 49.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 251.8 0 1 4 2 62.9 0 2 9 2 0.1 0 0 2 10 108 0.0 1 1 107 11 284 0.0 1 1 283 13 1 0.0 1 0 1 170 1 0.0 1 1 231 13 0.0 1 12 1 232 2 0.0 1 2 258 1 0.0 1 1 259 16 0.0 1 16 260 1 0.0 1 1 261 1 0.0 1 1 262 1 0.0 1 0 1 268 2 0.0 1 2 269 2 0.0 1 2 270 408 0.0 1 397 11 271 14 0.0 1 14 272 5 0.0 1 4 1