This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.83 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 131,527 (4.4%) Reads that were too short: 33,249 (1.1%) Reads written (passing filters): 98,278 (3.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 21,478,584 bp (2.4%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 131527 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 394 734.8 0 394 7 3676 734.8 0 3676 8 41804 734.8 0 41804 9 48 734.8 0 48 10 19 734.8 0 19 11 39 734.8 0 39 12 21 734.8 0 21 13 21 734.8 0 21 14 22 734.8 0 22 15 17 734.8 0 17 16 21 734.8 0 21 17 14 734.8 0 14 18 14 734.8 0 14 19 26 734.8 0 26 20 18 734.8 0 18 21 17 734.8 0 17 22 22 734.8 0 22 23 27 734.8 0 27 24 14 734.8 0 14 25 20 734.8 0 20 26 18 734.8 0 18 27 19 734.8 0 19 28 16 734.8 0 16 29 23 734.8 0 23 30 43 734.8 0 43 31 53 734.8 0 53 32 49 734.8 0 49 33 20 734.8 0 20 34 26 734.8 0 26 35 128 734.8 0 128 36 21 734.8 0 21 37 30 734.8 0 30 38 23 734.8 0 23 39 31 734.8 0 31 40 36 734.8 0 36 41 31 734.8 0 31 42 27 734.8 0 27 43 44 734.8 0 44 44 30 734.8 0 30 45 23 734.8 0 23 46 22 734.8 0 22 47 41 734.8 0 41 48 48 734.8 0 48 49 50 734.8 0 50 50 841 734.8 0 841 51 1235 734.8 0 1235 52 247 734.8 0 247 53 18 734.8 0 18 54 23 734.8 0 23 55 24 734.8 0 24 56 122 734.8 0 122 57 22 734.8 0 22 58 31 734.8 0 31 59 28 734.8 0 28 60 29 734.8 0 29 61 30 734.8 0 30 62 48 734.8 0 48 63 49 734.8 0 49 64 42 734.8 0 42 65 37 734.8 0 37 66 36 734.8 0 36 67 51 734.8 0 51 68 54 734.8 0 54 69 323 734.8 0 323 70 75 734.8 0 75 71 51 734.8 0 51 72 100 734.8 0 100 73 186 734.8 0 186 74 2464 734.8 0 2464 75 1522 734.8 0 1522 76 242 734.8 0 242 77 61 734.8 0 61 78 85 734.8 0 85 79 66 734.8 0 66 80 79 734.8 0 79 81 54 734.8 0 54 82 74 734.8 0 74 83 87 734.8 0 87 84 106 734.8 0 106 85 156 734.8 0 156 86 75 734.8 0 75 87 160 734.8 0 160 88 38 734.8 0 38 89 56 734.8 0 56 90 49 734.8 0 49 91 80 734.8 0 80 92 74 734.8 0 74 93 73 734.8 0 73 94 109 734.8 0 109 95 81 734.8 0 81 96 71 734.8 0 71 97 70 734.8 0 70 98 43 734.8 0 43 99 39 734.8 0 39 100 32 734.8 0 32 101 28 734.8 0 28 102 33 734.8 0 33 103 46 734.8 0 46 104 29 734.8 0 29 105 17 734.8 0 17 106 22 734.8 0 22 107 22 734.8 0 22 108 17 734.8 0 17 109 20 734.8 0 20 110 17 734.8 0 17 111 43 734.8 0 43 112 51 734.8 0 51 113 44 734.8 0 44 114 174 734.8 0 174 115 136 734.8 0 136 116 103 734.8 0 103 117 73 734.8 0 73 118 103 734.8 0 103 119 91 734.8 0 91 120 77 734.8 0 77 121 92 734.8 0 92 122 84 734.8 0 84 123 89 734.8 0 89 124 107 734.8 0 107 125 645 734.8 0 645 126 1155 734.8 0 1155 127 214 734.8 0 214 128 121 734.8 0 121 129 75 734.8 0 75 130 125 734.8 0 125 131 170 734.8 0 170 132 90 734.8 0 90 133 85 734.8 0 85 134 69 734.8 0 69 135 284 734.8 0 284 136 271 734.8 0 271 137 287 734.8 0 287 138 75 734.8 0 75 139 70 734.8 0 70 140 93 734.8 0 93 141 69 734.8 0 69 142 67 734.8 0 67 143 65 734.8 0 65 144 97 734.8 0 97 145 106 734.8 0 106 146 102 734.8 0 102 147 88 734.8 0 88 148 96 734.8 0 96 149 63 734.8 0 63 150 76 734.8 0 76 151 117 734.8 0 117 152 62 734.8 0 62 153 103 734.8 0 103 154 119 734.8 0 119 155 89 734.8 0 89 156 108 734.8 0 108 157 124 734.8 0 124 158 120 734.8 0 120 159 164 734.8 0 164 160 190 734.8 0 190 161 164 734.8 0 164 162 185 734.8 0 185 163 434 734.8 0 434 164 11873 734.8 0 11873 165 5891 734.8 0 5891 166 667 734.8 0 667 167 88 734.8 0 88 168 87 734.8 0 87 169 120 734.8 0 120 170 109 734.8 0 109 171 105 734.8 0 105 172 101 734.8 0 101 173 106 734.8 0 106 174 88 734.8 0 88 175 102 734.8 0 102 176 95 734.8 0 95 177 70 734.8 0 70 178 65 734.8 0 65 179 116 734.8 0 116 180 109 734.8 0 109 181 127 734.8 0 127 182 110 734.8 0 110 183 95 734.8 0 95 184 98 734.8 0 98 185 119 734.8 0 119 186 162 734.8 0 162 187 101 734.8 0 101 188 90 734.8 0 90 189 110 734.8 0 110 190 93 734.8 0 93 191 181 734.8 0 181 192 547 734.8 0 547 193 319 734.8 0 319 194 163 734.8 0 163 195 310 734.8 0 310 196 1218 734.8 0 1218 197 2223 734.8 0 2223 198 5062 734.8 0 5062 199 1440 734.8 0 1440 200 680 734.8 0 680 201 229 734.8 0 229 202 107 734.8 0 107 203 57 734.8 0 57 204 119 734.8 0 119 205 344 734.8 0 344 206 109 734.8 0 109 207 86 734.8 0 86 208 72 734.8 0 72 209 72 734.8 0 72 210 83 734.8 0 83 211 78 734.8 0 78 212 101 734.8 0 101 213 98 734.8 0 98 214 126 734.8 0 126 215 137 734.8 0 137 216 398 734.8 0 398 217 967 734.8 0 967 218 805 734.8 0 805 219 236 734.8 0 236 220 198 734.8 0 198 221 241 734.8 0 241 222 100 734.8 0 100 223 71 734.8 0 71 224 105 734.8 0 105 225 107 734.8 0 107 226 97 734.8 0 97 227 111 734.8 0 111 228 115 734.8 0 115 229 156 734.8 0 156 230 115 734.8 0 115 231 90 734.8 0 90 232 68 734.8 0 68 233 65 734.8 0 65 234 62 734.8 0 62 235 123 734.8 0 123 236 108 734.8 0 108 237 217 734.8 0 217 238 180 734.8 0 180 239 499 734.8 0 499 240 357 734.8 0 357 241 163 734.8 0 163 242 73 734.8 0 73 243 57 734.8 0 57 244 75 734.8 0 75 245 113 734.8 0 113 246 291 734.8 0 291 247 3385 734.8 0 3385 248 8550 734.8 0 8550 249 2185 734.8 0 2185 250 710 734.8 0 710 251 116 734.8 0 116 252 44 734.8 0 44 253 42 734.8 0 42 254 112 734.8 0 112 255 116 734.8 0 116 256 123 734.8 0 123 257 188 734.8 0 188 258 224 734.8 0 224 259 117 734.8 0 117 260 69 734.8 0 69 261 60 734.8 0 60 262 42 734.8 0 42 263 35 734.8 0 35 264 41 734.8 0 41 265 75 734.8 0 75 266 101 734.8 0 101 267 113 734.8 0 113 268 151 734.8 0 151 269 128 734.8 0 128 270 125 734.8 0 125 271 49 734.8 0 49 272 71 734.8 0 71 273 57 734.8 0 57 274 87 734.8 0 87 275 103 734.8 0 103 276 99 734.8 0 99 277 156 734.8 0 156 278 117 734.8 0 117 279 290 734.8 0 290 280 698 734.8 0 698 281 631 734.8 0 631 282 407 734.8 0 407 283 107 734.8 0 107 284 109 734.8 0 109 285 150 734.8 0 150 286 190 734.8 0 190 287 221 734.8 0 221 288 170 734.8 0 170 289 469 734.8 0 469 290 1005 734.8 0 1005 291 944 734.8 0 944 292 675 734.8 0 675 293 283 734.8 0 283 294 297 734.8 0 297 295 150 734.8 0 150 296 112 734.8 0 112 297 156 734.8 0 156 298 237 734.8 0 237 299 130 734.8 0 130 300 110 734.8 0 110 301 165 734.8 0 165 Finished in 3.78 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 98,278 Reads with adapters: 3,999 (4.1%) Reads that were too short: 279 (0.3%) Reads written (passing filters): 3,720 (3.8%) Total basepairs processed: 21,478,584 bp Total written (filtered): 957,829 bp (4.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3999 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 25 0.0 3 0 22 1 2 17 3221 0.0 3 3118 88 13 2 18 24 0.0 3 1 8 2 13 19 5 0.0 3 1 0 2 2 20 4 0.0 3 0 0 0 4 24 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 27 213 0.0 3 0 0 2 211 28 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 30 39 0.0 3 0 0 0 39 39 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 14 0.0 3 0 0 0 14 49 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 78 3 0.0 3 0 0 0 3 79 2 0.0 3 0 0 0 2 84 3 0.0 3 0 0 0 3 86 1 0.0 3 0 0 0 1 87 53 0.0 3 0 0 0 53 88 13 0.0 3 0 0 1 12 89 8 0.0 3 0 0 0 8 90 97 0.0 3 0 0 4 93 91 10 0.0 3 0 0 0 10 92 2 0.0 3 0 0 0 2 94 3 0.0 3 0 0 1 2 95 2 0.0 3 0 0 0 2 97 12 0.0 3 0 0 1 11 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 63 0.0 3 0 0 3 60 118 9 0.0 3 0 0 0 9 119 6 0.0 3 0 0 0 6 120 47 0.0 3 0 0 0 47 121 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 158 7 0.0 3 0 0 0 7 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 0 7 205 1 0.0 3 0 0 1 207 1 0.0 3 0 0 0 1 209 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 21 0.0 3 0 0 0 21 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 8 0.0 3 0 0 0 8 282 5 0.0 3 0 0 0 5 283 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 3,720 Reads with adapters: 366 (9.8%) Reads written (passing filters): 3,720 (100.0%) Total basepairs processed: 957,829 bp Total written (filtered): 956,731 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 366 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.7% C: 60.1% G: 8.2% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 366 58.1 0 366