This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.71 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 33,288 (1.1%) Reads that were too short: 4,951 (0.2%) Reads written (passing filters): 28,337 (0.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 5,046,103 bp (0.6%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 33288 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 50 734.8 0 50 7 2994 734.8 0 2994 8 35 734.8 0 35 9 41 734.8 0 41 10 15 734.8 0 15 11 30 734.8 0 30 12 21 734.8 0 21 13 21 734.8 0 21 14 13 734.8 0 13 15 30 734.8 0 30 16 18 734.8 0 18 17 18 734.8 0 18 18 26 734.8 0 26 19 31 734.8 0 31 20 25 734.8 0 25 21 23 734.8 0 23 22 31 734.8 0 31 23 20 734.8 0 20 24 18 734.8 0 18 25 22 734.8 0 22 26 23 734.8 0 23 27 27 734.8 0 27 28 35 734.8 0 35 29 23 734.8 0 23 30 751 734.8 0 751 31 100 734.8 0 100 32 29 734.8 0 29 33 348 734.8 0 348 34 287 734.8 0 287 35 95 734.8 0 95 36 20 734.8 0 20 37 22 734.8 0 22 38 30 734.8 0 30 39 27 734.8 0 27 40 18 734.8 0 18 41 28 734.8 0 28 42 15 734.8 0 15 43 26 734.8 0 26 44 29 734.8 0 29 45 30 734.8 0 30 46 27 734.8 0 27 47 28 734.8 0 28 48 26 734.8 0 26 49 23 734.8 0 23 50 32 734.8 0 32 51 18 734.8 0 18 52 20 734.8 0 20 53 24 734.8 0 24 54 21 734.8 0 21 55 53 734.8 0 53 56 49 734.8 0 49 57 116 734.8 0 116 58 254 734.8 0 254 59 34 734.8 0 34 60 27 734.8 0 27 61 26 734.8 0 26 62 21 734.8 0 21 63 22 734.8 0 22 64 27 734.8 0 27 65 39 734.8 0 39 66 25 734.8 0 25 67 22 734.8 0 22 68 20 734.8 0 20 69 23 734.8 0 23 70 24 734.8 0 24 71 21 734.8 0 21 72 187 734.8 0 187 73 157 734.8 0 157 74 31 734.8 0 31 75 19 734.8 0 19 76 28 734.8 0 28 77 24 734.8 0 24 78 32 734.8 0 32 79 17 734.8 0 17 80 17 734.8 0 17 81 13 734.8 0 13 82 17 734.8 0 17 83 21 734.8 0 21 84 91 734.8 0 91 85 131 734.8 0 131 86 59 734.8 0 59 87 25 734.8 0 25 88 19 734.8 0 19 89 26 734.8 0 26 90 18 734.8 0 18 91 22 734.8 0 22 92 22 734.8 0 22 93 22 734.8 0 22 94 41 734.8 0 41 95 26 734.8 0 26 96 28 734.8 0 28 97 23 734.8 0 23 98 46 734.8 0 46 99 28 734.8 0 28 100 31 734.8 0 31 101 29 734.8 0 29 102 48 734.8 0 48 103 31 734.8 0 31 104 34 734.8 0 34 105 241 734.8 0 241 106 140 734.8 0 140 107 725 734.8 0 725 108 1094 734.8 0 1094 109 423 734.8 0 423 110 21 734.8 0 21 111 27 734.8 0 27 112 20 734.8 0 20 113 22 734.8 0 22 114 22 734.8 0 22 115 23 734.8 0 23 116 30 734.8 0 30 117 21 734.8 0 21 118 14 734.8 0 14 119 21 734.8 0 21 120 34 734.8 0 34 121 43 734.8 0 43 122 49 734.8 0 49 123 21 734.8 0 21 124 29 734.8 0 29 125 37 734.8 0 37 126 21 734.8 0 21 127 287 734.8 0 287 128 360 734.8 0 360 129 102 734.8 0 102 130 55 734.8 0 55 131 26 734.8 0 26 132 30 734.8 0 30 133 31 734.8 0 31 134 36 734.8 0 36 135 55 734.8 0 55 136 126 734.8 0 126 137 111 734.8 0 111 138 75 734.8 0 75 139 43 734.8 0 43 140 125 734.8 0 125 141 158 734.8 0 158 142 125 734.8 0 125 143 527 734.8 0 527 144 3030 734.8 0 3030 145 319 734.8 0 319 146 57 734.8 0 57 147 53 734.8 0 53 148 35 734.8 0 35 149 48 734.8 0 48 150 36 734.8 0 36 151 39 734.8 0 39 152 60 734.8 0 60 153 314 734.8 0 314 154 482 734.8 0 482 155 165 734.8 0 165 156 54 734.8 0 54 157 38 734.8 0 38 158 78 734.8 0 78 159 49 734.8 0 49 160 42 734.8 0 42 161 34 734.8 0 34 162 36 734.8 0 36 163 28 734.8 0 28 164 40 734.8 0 40 165 39 734.8 0 39 166 61 734.8 0 61 167 51 734.8 0 51 168 76 734.8 0 76 169 291 734.8 0 291 170 2622 734.8 0 2622 171 261 734.8 0 261 172 30 734.8 0 30 173 32 734.8 0 32 174 51 734.8 0 51 175 54 734.8 0 54 176 49 734.8 0 49 177 34 734.8 0 34 178 26 734.8 0 26 179 30 734.8 0 30 180 51 734.8 0 51 181 68 734.8 0 68 182 88 734.8 0 88 183 78 734.8 0 78 184 107 734.8 0 107 185 172 734.8 0 172 186 2619 734.8 0 2619 187 2355 734.8 0 2355 188 540 734.8 0 540 189 35 734.8 0 35 190 15 734.8 0 15 191 34 734.8 0 34 192 22 734.8 0 22 193 16 734.8 0 16 194 22 734.8 0 22 195 24 734.8 0 24 196 28 734.8 0 28 197 30 734.8 0 30 198 31 734.8 0 31 199 26 734.8 0 26 200 22 734.8 0 22 201 30 734.8 0 30 202 27 734.8 0 27 203 34 734.8 0 34 204 86 734.8 0 86 205 112 734.8 0 112 206 36 734.8 0 36 207 29 734.8 0 29 208 48 734.8 0 48 209 48 734.8 0 48 210 38 734.8 0 38 211 21 734.8 0 21 212 40 734.8 0 40 213 27 734.8 0 27 214 27 734.8 0 27 215 35 734.8 0 35 216 45 734.8 0 45 217 147 734.8 0 147 218 333 734.8 0 333 219 215 734.8 0 215 220 18 734.8 0 18 221 35 734.8 0 35 222 68 734.8 0 68 223 50 734.8 0 50 224 33 734.8 0 33 225 33 734.8 0 33 226 51 734.8 0 51 227 95 734.8 0 95 228 30 734.8 0 30 229 27 734.8 0 27 230 21 734.8 0 21 231 31 734.8 0 31 232 35 734.8 0 35 233 34 734.8 0 34 234 19 734.8 0 19 235 45 734.8 0 45 236 32 734.8 0 32 237 34 734.8 0 34 238 33 734.8 0 33 239 24 734.8 0 24 240 25 734.8 0 25 241 26 734.8 0 26 242 32 734.8 0 32 243 34 734.8 0 34 244 43 734.8 0 43 245 44 734.8 0 44 246 29 734.8 0 29 247 27 734.8 0 27 248 23 734.8 0 23 249 19 734.8 0 19 250 73 734.8 0 73 251 45 734.8 0 45 252 41 734.8 0 41 253 69 734.8 0 69 254 64 734.8 0 64 255 40 734.8 0 40 256 25 734.8 0 25 257 42 734.8 0 42 258 36 734.8 0 36 259 34 734.8 0 34 260 33 734.8 0 33 261 43 734.8 0 43 262 95 734.8 0 95 263 145 734.8 0 145 264 44 734.8 0 44 265 41 734.8 0 41 266 59 734.8 0 59 267 35 734.8 0 35 268 37 734.8 0 37 269 23 734.8 0 23 270 31 734.8 0 31 271 39 734.8 0 39 272 28 734.8 0 28 273 36 734.8 0 36 274 41 734.8 0 41 275 32 734.8 0 32 276 26 734.8 0 26 277 54 734.8 0 54 278 153 734.8 0 153 279 75 734.8 0 75 280 65 734.8 0 65 281 79 734.8 0 79 282 51 734.8 0 51 283 34 734.8 0 34 284 26 734.8 0 26 285 95 734.8 0 95 286 63 734.8 0 63 287 30 734.8 0 30 288 37 734.8 0 37 289 23 734.8 0 23 290 40 734.8 0 40 291 29 734.8 0 29 292 35 734.8 0 35 293 19 734.8 0 19 294 41 734.8 0 41 295 35 734.8 0 35 296 30 734.8 0 30 297 66 734.8 0 66 298 94 734.8 0 94 299 51 734.8 0 51 300 44 734.8 0 44 301 27 734.8 0 27 Finished in 1.08 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 28,337 Reads with adapters: 3,026 (10.7%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 3,021 (10.7%) Total basepairs processed: 5,046,103 bp Total written (filtered): 825,177 bp (16.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3026 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 1 19 33 0.0 3 0 31 1 1 20 2950 0.0 4 2903 43 2 0 2 21 5 0.0 4 2 3 23 3 0.0 4 0 0 0 3 111 27 0.0 4 0 0 0 24 3 146 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 1 213 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 3,021 Reads with adapters: 16 (0.5%) Reads written (passing filters): 3,021 (100.0%) Total basepairs processed: 825,177 bp Total written (filtered): 823,710 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 16 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 18.8% C: 18.8% G: 62.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 10 1 0.0 1 0 1 11 7 0.0 1 1 6 12 2 0.0 1 0 2 152 2 0.0 1 0 2 261 3 0.0 1 3 269 1 0.0 1 1