This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.55 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 173,807 (5.8%) Reads that were too short: 80,499 (2.7%) Reads written (passing filters): 93,308 (3.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 21,347,108 bp (2.4%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 173807 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 417 734.8 0 417 7 43232 734.8 0 43232 8 94 734.8 0 94 9 3993 734.8 0 3993 10 32 734.8 0 32 11 23 734.8 0 23 12 21 734.8 0 21 13 25 734.8 0 25 14 25 734.8 0 25 15 80 734.8 0 80 16 47 734.8 0 47 17 23 734.8 0 23 18 35 734.8 0 35 19 39 734.8 0 39 20 17 734.8 0 17 21 28 734.8 0 28 22 40 734.8 0 40 23 27 734.8 0 27 24 30 734.8 0 30 25 30 734.8 0 30 26 20 734.8 0 20 27 35 734.8 0 35 28 39 734.8 0 39 29 31 734.8 0 31 30 35 734.8 0 35 31 26 734.8 0 26 32 26 734.8 0 26 33 27 734.8 0 27 34 25 734.8 0 25 35 33 734.8 0 33 36 27 734.8 0 27 37 25 734.8 0 25 38 40 734.8 0 40 39 26 734.8 0 26 40 31 734.8 0 31 41 27 734.8 0 27 42 25 734.8 0 25 43 30 734.8 0 30 44 21 734.8 0 21 45 21 734.8 0 21 46 18 734.8 0 18 47 29 734.8 0 29 48 36 734.8 0 36 49 29 734.8 0 29 50 35 734.8 0 35 51 28 734.8 0 28 52 17 734.8 0 17 53 32 734.8 0 32 54 27 734.8 0 27 55 42 734.8 0 42 56 38 734.8 0 38 57 257 734.8 0 257 58 411 734.8 0 411 59 124 734.8 0 124 60 25 734.8 0 25 61 23 734.8 0 23 62 31 734.8 0 31 63 37 734.8 0 37 64 154 734.8 0 154 65 44 734.8 0 44 66 14 734.8 0 14 67 25 734.8 0 25 68 33 734.8 0 33 69 33 734.8 0 33 70 22 734.8 0 22 71 19 734.8 0 19 72 61 734.8 0 61 73 43 734.8 0 43 74 99 734.8 0 99 75 77 734.8 0 77 76 62 734.8 0 62 77 87 734.8 0 87 78 62 734.8 0 62 79 104 734.8 0 104 80 120 734.8 0 120 81 68 734.8 0 68 82 153 734.8 0 153 83 162 734.8 0 162 84 195 734.8 0 195 85 193 734.8 0 193 86 169 734.8 0 169 87 144 734.8 0 144 88 706 734.8 0 706 89 505 734.8 0 505 90 645 734.8 0 645 91 483 734.8 0 483 92 445 734.8 0 445 93 97 734.8 0 97 94 153 734.8 0 153 95 141 734.8 0 141 96 113 734.8 0 113 97 96 734.8 0 96 98 116 734.8 0 116 99 139 734.8 0 139 100 128 734.8 0 128 101 186 734.8 0 186 102 181 734.8 0 181 103 131 734.8 0 131 104 231 734.8 0 231 105 170 734.8 0 170 106 96 734.8 0 96 107 102 734.8 0 102 108 241 734.8 0 241 109 216 734.8 0 216 110 126 734.8 0 126 111 85 734.8 0 85 112 139 734.8 0 139 113 128 734.8 0 128 114 142 734.8 0 142 115 236 734.8 0 236 116 244 734.8 0 244 117 154 734.8 0 154 118 77 734.8 0 77 119 99 734.8 0 99 120 113 734.8 0 113 121 307 734.8 0 307 122 1101 734.8 0 1101 123 848 734.8 0 848 124 320 734.8 0 320 125 164 734.8 0 164 126 166 734.8 0 166 127 191 734.8 0 191 128 187 734.8 0 187 129 190 734.8 0 190 130 273 734.8 0 273 131 298 734.8 0 298 132 175 734.8 0 175 133 136 734.8 0 136 134 245 734.8 0 245 135 251 734.8 0 251 136 296 734.8 0 296 137 214 734.8 0 214 138 190 734.8 0 190 139 182 734.8 0 182 140 298 734.8 0 298 141 1098 734.8 0 1098 142 975 734.8 0 975 143 480 734.8 0 480 144 164 734.8 0 164 145 185 734.8 0 185 146 275 734.8 0 275 147 446 734.8 0 446 148 754 734.8 0 754 149 331 734.8 0 331 150 329 734.8 0 329 151 736 734.8 0 736 152 2668 734.8 0 2668 153 501 734.8 0 501 154 380 734.8 0 380 155 352 734.8 0 352 156 292 734.8 0 292 157 652 734.8 0 652 158 754 734.8 0 754 159 396 734.8 0 396 160 294 734.8 0 294 161 369 734.8 0 369 162 557 734.8 0 557 163 369 734.8 0 369 164 196 734.8 0 196 165 221 734.8 0 221 166 331 734.8 0 331 167 404 734.8 0 404 168 327 734.8 0 327 169 310 734.8 0 310 170 437 734.8 0 437 171 4940 734.8 0 4940 172 255 734.8 0 255 173 114 734.8 0 114 174 172 734.8 0 172 175 323 734.8 0 323 176 332 734.8 0 332 177 237 734.8 0 237 178 185 734.8 0 185 179 133 734.8 0 133 180 137 734.8 0 137 181 194 734.8 0 194 182 528 734.8 0 528 183 634 734.8 0 634 184 337 734.8 0 337 185 226 734.8 0 226 186 331 734.8 0 331 187 384 734.8 0 384 188 341 734.8 0 341 189 238 734.8 0 238 190 179 734.8 0 179 191 177 734.8 0 177 192 144 734.8 0 144 193 193 734.8 0 193 194 170 734.8 0 170 195 238 734.8 0 238 196 458 734.8 0 458 197 293 734.8 0 293 198 286 734.8 0 286 199 147 734.8 0 147 200 116 734.8 0 116 201 177 734.8 0 177 202 250 734.8 0 250 203 354 734.8 0 354 204 274 734.8 0 274 205 290 734.8 0 290 206 292 734.8 0 292 207 334 734.8 0 334 208 547 734.8 0 547 209 1194 734.8 0 1194 210 1268 734.8 0 1268 211 943 734.8 0 943 212 908 734.8 0 908 213 12293 734.8 0 12293 214 20757 734.8 0 20757 215 3727 734.8 0 3727 216 409 734.8 0 409 217 313 734.8 0 313 218 307 734.8 0 307 219 233 734.8 0 233 220 223 734.8 0 223 221 2099 734.8 0 2099 222 3153 734.8 0 3153 223 1362 734.8 0 1362 224 372 734.8 0 372 225 224 734.8 0 224 226 321 734.8 0 321 227 291 734.8 0 291 228 242 734.8 0 242 229 192 734.8 0 192 230 132 734.8 0 132 231 246 734.8 0 246 232 286 734.8 0 286 233 302 734.8 0 302 234 336 734.8 0 336 235 432 734.8 0 432 236 210 734.8 0 210 237 218 734.8 0 218 238 171 734.8 0 171 239 172 734.8 0 172 240 153 734.8 0 153 241 135 734.8 0 135 242 250 734.8 0 250 243 563 734.8 0 563 244 978 734.8 0 978 245 540 734.8 0 540 246 283 734.8 0 283 247 212 734.8 0 212 248 111 734.8 0 111 249 62 734.8 0 62 250 66 734.8 0 66 251 158 734.8 0 158 252 282 734.8 0 282 253 420 734.8 0 420 254 390 734.8 0 390 255 401 734.8 0 401 256 437 734.8 0 437 257 1786 734.8 0 1786 258 2363 734.8 0 2363 259 559 734.8 0 559 260 162 734.8 0 162 261 221 734.8 0 221 262 229 734.8 0 229 263 510 734.8 0 510 264 705 734.8 0 705 265 482 734.8 0 482 266 311 734.8 0 311 267 268 734.8 0 268 268 239 734.8 0 239 269 143 734.8 0 143 270 273 734.8 0 273 271 695 734.8 0 695 272 647 734.8 0 647 273 507 734.8 0 507 274 313 734.8 0 313 275 245 734.8 0 245 276 229 734.8 0 229 277 208 734.8 0 208 278 575 734.8 0 575 279 1169 734.8 0 1169 280 267 734.8 0 267 281 235 734.8 0 235 282 412 734.8 0 412 283 285 734.8 0 285 284 745 734.8 0 745 285 1168 734.8 0 1168 286 445 734.8 0 445 287 168 734.8 0 168 288 88 734.8 0 88 289 110 734.8 0 110 290 233 734.8 0 233 291 435 734.8 0 435 292 331 734.8 0 331 293 172 734.8 0 172 294 204 734.8 0 204 295 251 734.8 0 251 296 166 734.8 0 166 297 167 734.8 0 167 298 206 734.8 0 206 299 196 734.8 0 196 300 111 734.8 0 111 301 147 734.8 0 147 Finished in 3.66 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 93,308 Reads with adapters: 4,126 (4.4%) Reads that were too short: 66 (0.1%) Reads written (passing filters): 4,060 (4.4%) Total basepairs processed: 21,347,108 bp Total written (filtered): 1,111,851 bp (5.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4126 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 52 0.0 3 0 50 2 17 3921 0.0 3 3815 90 11 5 18 13 0.0 3 0 8 2 3 19 5 0.0 3 2 0 1 2 20 37 0.0 3 28 1 0 8 25 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 35 3 0.0 3 0 0 0 3 37 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 7 0.0 3 0 0 0 7 146 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 195 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 217 6 0.0 3 0 0 0 6 222 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 281 11 0.0 3 0 0 0 11 282 7 0.0 3 0 0 0 7 283 3 0.0 3 0 0 1 2 284 1 0.0 3 0 0 0 1 288 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 4,060 Reads with adapters: 159 (3.9%) Reads written (passing filters): 4,060 (100.0%) Total basepairs processed: 1,111,851 bp Total written (filtered): 1,111,368 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 159 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.5% C: 13.8% G: 28.9% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 153 63.4 0 153 4 6 15.9 0 6