This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.85 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 405,951 (13.5%) Reads that were too short: 6,238 (0.2%) Reads written (passing filters): 399,713 (13.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 104,014,849 bp (11.5%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 405951 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 836 734.8 0 836 7 1133 734.8 0 1133 8 67256 734.8 0 67256 9 261 734.8 0 261 10 147 734.8 0 147 11 116 734.8 0 116 12 112 734.8 0 112 13 317 734.8 0 317 14 216 734.8 0 216 15 267 734.8 0 267 16 240 734.8 0 240 17 355 734.8 0 355 18 418 734.8 0 418 19 908 734.8 0 908 20 34170 734.8 0 34170 21 29259 734.8 0 29259 22 6619 734.8 0 6619 23 115 734.8 0 115 24 55 734.8 0 55 25 121 734.8 0 121 26 83 734.8 0 83 27 72 734.8 0 72 28 47 734.8 0 47 29 95 734.8 0 95 30 59 734.8 0 59 31 106 734.8 0 106 32 155 734.8 0 155 33 295 734.8 0 295 34 242 734.8 0 242 35 431 734.8 0 431 36 1059 734.8 0 1059 37 296 734.8 0 296 38 549 734.8 0 549 39 732 734.8 0 732 40 526 734.8 0 526 41 503 734.8 0 503 42 829 734.8 0 829 43 678 734.8 0 678 44 1048 734.8 0 1048 45 1033 734.8 0 1033 46 1408 734.8 0 1408 47 1678 734.8 0 1678 48 3960 734.8 0 3960 49 127406 734.8 0 127406 50 87064 734.8 0 87064 51 11646 734.8 0 11646 52 160 734.8 0 160 53 77 734.8 0 77 54 47 734.8 0 47 55 55 734.8 0 55 56 65 734.8 0 65 57 88 734.8 0 88 58 45 734.8 0 45 59 29 734.8 0 29 60 39 734.8 0 39 61 80 734.8 0 80 62 175 734.8 0 175 63 179 734.8 0 179 64 275 734.8 0 275 65 65 734.8 0 65 66 18 734.8 0 18 67 27 734.8 0 27 68 41 734.8 0 41 69 28 734.8 0 28 70 31 734.8 0 31 71 37 734.8 0 37 72 36 734.8 0 36 73 41 734.8 0 41 74 45 734.8 0 45 75 33 734.8 0 33 76 24 734.8 0 24 77 27 734.8 0 27 78 37 734.8 0 37 79 40 734.8 0 40 80 31 734.8 0 31 81 25 734.8 0 25 82 38 734.8 0 38 83 38 734.8 0 38 84 52 734.8 0 52 85 54 734.8 0 54 86 41 734.8 0 41 87 35 734.8 0 35 88 27 734.8 0 27 89 26 734.8 0 26 90 37 734.8 0 37 91 23 734.8 0 23 92 19 734.8 0 19 93 33 734.8 0 33 94 31 734.8 0 31 95 30 734.8 0 30 96 30 734.8 0 30 97 33 734.8 0 33 98 38 734.8 0 38 99 34 734.8 0 34 100 17 734.8 0 17 101 19 734.8 0 19 102 35 734.8 0 35 103 32 734.8 0 32 104 36 734.8 0 36 105 29 734.8 0 29 106 39 734.8 0 39 107 28 734.8 0 28 108 26 734.8 0 26 109 25 734.8 0 25 110 32 734.8 0 32 111 31 734.8 0 31 112 31 734.8 0 31 113 33 734.8 0 33 114 37 734.8 0 37 115 39 734.8 0 39 116 51 734.8 0 51 117 212 734.8 0 212 118 136 734.8 0 136 119 49 734.8 0 49 120 30 734.8 0 30 121 30 734.8 0 30 122 34 734.8 0 34 123 22 734.8 0 22 124 48 734.8 0 48 125 63 734.8 0 63 126 27 734.8 0 27 127 39 734.8 0 39 128 27 734.8 0 27 129 29 734.8 0 29 130 38 734.8 0 38 131 22 734.8 0 22 132 27 734.8 0 27 133 31 734.8 0 31 134 30 734.8 0 30 135 38 734.8 0 38 136 27 734.8 0 27 137 34 734.8 0 34 138 56 734.8 0 56 139 40 734.8 0 40 140 27 734.8 0 27 141 40 734.8 0 40 142 42 734.8 0 42 143 25 734.8 0 25 144 52 734.8 0 52 145 37 734.8 0 37 146 40 734.8 0 40 147 33 734.8 0 33 148 40 734.8 0 40 149 40 734.8 0 40 150 34 734.8 0 34 151 38 734.8 0 38 152 42 734.8 0 42 153 33 734.8 0 33 154 52 734.8 0 52 155 35 734.8 0 35 156 43 734.8 0 43 157 55 734.8 0 55 158 48 734.8 0 48 159 44 734.8 0 44 160 30 734.8 0 30 161 32 734.8 0 32 162 24 734.8 0 24 163 29 734.8 0 29 164 34 734.8 0 34 165 35 734.8 0 35 166 32 734.8 0 32 167 33 734.8 0 33 168 30 734.8 0 30 169 28 734.8 0 28 170 33 734.8 0 33 171 33 734.8 0 33 172 39 734.8 0 39 173 34 734.8 0 34 174 47 734.8 0 47 175 50 734.8 0 50 176 43 734.8 0 43 177 38 734.8 0 38 178 46 734.8 0 46 179 46 734.8 0 46 180 81 734.8 0 81 181 58 734.8 0 58 182 198 734.8 0 198 183 313 734.8 0 313 184 241 734.8 0 241 185 53 734.8 0 53 186 63 734.8 0 63 187 50 734.8 0 50 188 61 734.8 0 61 189 63 734.8 0 63 190 65 734.8 0 65 191 77 734.8 0 77 192 121 734.8 0 121 193 1933 734.8 0 1933 194 2971 734.8 0 2971 195 2261 734.8 0 2261 196 222 734.8 0 222 197 113 734.8 0 113 198 62 734.8 0 62 199 27 734.8 0 27 200 42 734.8 0 42 201 53 734.8 0 53 202 32 734.8 0 32 203 27 734.8 0 27 204 27 734.8 0 27 205 42 734.8 0 42 206 52 734.8 0 52 207 40 734.8 0 40 208 41 734.8 0 41 209 31 734.8 0 31 210 35 734.8 0 35 211 39 734.8 0 39 212 39 734.8 0 39 213 48 734.8 0 48 214 103 734.8 0 103 215 138 734.8 0 138 216 50 734.8 0 50 217 48 734.8 0 48 218 42 734.8 0 42 219 149 734.8 0 149 220 117 734.8 0 117 221 76 734.8 0 76 222 63 734.8 0 63 223 30 734.8 0 30 224 48 734.8 0 48 225 41 734.8 0 41 226 28 734.8 0 28 227 36 734.8 0 36 228 26 734.8 0 26 229 25 734.8 0 25 230 26 734.8 0 26 231 26 734.8 0 26 232 31 734.8 0 31 233 20 734.8 0 20 234 45 734.8 0 45 235 78 734.8 0 78 236 45 734.8 0 45 237 44 734.8 0 44 238 52 734.8 0 52 239 201 734.8 0 201 240 167 734.8 0 167 241 109 734.8 0 109 242 37 734.8 0 37 243 26 734.8 0 26 244 49 734.8 0 49 245 42 734.8 0 42 246 52 734.8 0 52 247 57 734.8 0 57 248 39 734.8 0 39 249 38 734.8 0 38 250 49 734.8 0 49 251 71 734.8 0 71 252 46 734.8 0 46 253 25 734.8 0 25 254 39 734.8 0 39 255 45 734.8 0 45 256 72 734.8 0 72 257 52 734.8 0 52 258 49 734.8 0 49 259 43 734.8 0 43 260 48 734.8 0 48 261 39 734.8 0 39 262 34 734.8 0 34 263 88 734.8 0 88 264 174 734.8 0 174 265 67 734.8 0 67 266 66 734.8 0 66 267 44 734.8 0 44 268 43 734.8 0 43 269 46 734.8 0 46 270 31 734.8 0 31 271 28 734.8 0 28 272 40 734.8 0 40 273 39 734.8 0 39 274 45 734.8 0 45 275 53 734.8 0 53 276 38 734.8 0 38 277 46 734.8 0 46 278 35 734.8 0 35 279 38 734.8 0 38 280 37 734.8 0 37 281 32 734.8 0 32 282 32 734.8 0 32 283 36 734.8 0 36 284 34 734.8 0 34 285 46 734.8 0 46 286 58 734.8 0 58 287 65 734.8 0 65 288 471 734.8 0 471 289 446 734.8 0 446 290 186 734.8 0 186 291 36 734.8 0 36 292 38 734.8 0 38 293 96 734.8 0 96 294 69 734.8 0 69 295 41 734.8 0 41 296 24 734.8 0 24 297 26 734.8 0 26 298 30 734.8 0 30 299 23 734.8 0 23 300 113 734.8 0 113 301 109 734.8 0 109 Finished in 18.85 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 399,713 Reads with adapters: 74,491 (18.6%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 74,461 (18.6%) Total basepairs processed: 104,014,849 bp Total written (filtered): 19,739,507 bp (19.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 74491 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 38 0.0 3 0 0 38 19 886 0.0 3 0 871 9 3 3 20 67801 0.0 4 67076 701 20 3 1 21 130 0.0 4 28 101 1 22 9 0.0 4 0 0 7 1 1 23 22 0.0 4 0 0 0 18 4 25 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 50 2 0.0 4 0 0 0 0 2 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 78 1 0.0 4 0 0 0 1 79 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 6 0.0 4 0 0 0 0 6 84 5 0.0 4 0 0 0 0 5 85 4730 0.0 4 0 0 0 0 4730 108 1 0.0 4 0 0 0 0 1 111 16 0.0 4 0 0 0 13 3 112 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 0 2 114 796 0.0 4 0 0 0 0 796 138 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 186 5 0.0 4 0 0 0 0 5 198 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 223 3 0.0 4 0 0 0 0 3 240 2 0.0 4 0 0 0 0 2 243 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 1 245 1 0.0 4 0 0 0 0 1 246 4 0.0 4 0 0 0 0 4 249 1 0.0 4 0 0 0 0 1 252 3 0.0 4 0 0 0 0 3 269 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.48 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 74,461 Reads with adapters: 2,170 (2.9%) Reads written (passing filters): 74,461 (100.0%) Total basepairs processed: 19,739,507 bp Total written (filtered): 19,702,238 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2170 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.0% C: 1.1% G: 94.1% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 1163.5 0 8 4 18 290.9 0 18 5 5 72.7 0 5 6 1 18.2 0 1 9 4 0.3 0 1 3 10 279 0.1 1 4 275 11 1793 0.0 1 10 1783 14 3 0.0 1 0 3 71 1 0.0 1 1 139 1 0.0 1 1 151 2 0.0 1 0 2 154 1 0.0 1 1 224 3 0.0 1 2 1 258 2 0.0 1 2 259 45 0.0 1 44 1 260 2 0.0 1 2 261 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.