This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.30 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 287,867 (9.6%) Reads that were too short: 5,274 (0.2%) Reads written (passing filters): 282,593 (9.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 78,960,053 bp (8.7%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 287867 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2943 734.8 0 2943 7 5930 734.8 0 5930 8 240214 734.8 0 240214 9 566 734.8 0 566 10 65 734.8 0 65 11 35 734.8 0 35 12 41 734.8 0 41 13 40 734.8 0 40 14 37 734.8 0 37 15 42 734.8 0 42 16 78 734.8 0 78 17 42 734.8 0 42 18 50 734.8 0 50 19 105 734.8 0 105 20 96 734.8 0 96 21 92 734.8 0 92 22 27 734.8 0 27 23 37 734.8 0 37 24 31 734.8 0 31 25 36 734.8 0 36 26 60 734.8 0 60 27 42 734.8 0 42 28 24 734.8 0 24 29 46 734.8 0 46 30 52 734.8 0 52 31 50 734.8 0 50 32 34 734.8 0 34 33 40 734.8 0 40 34 33 734.8 0 33 35 41 734.8 0 41 36 40 734.8 0 40 37 41 734.8 0 41 38 44 734.8 0 44 39 54 734.8 0 54 40 69 734.8 0 69 41 785 734.8 0 785 42 51 734.8 0 51 43 26 734.8 0 26 44 47 734.8 0 47 45 40 734.8 0 40 46 33 734.8 0 33 47 43 734.8 0 43 48 44 734.8 0 44 49 47 734.8 0 47 50 46 734.8 0 46 51 43 734.8 0 43 52 43 734.8 0 43 53 37 734.8 0 37 54 35 734.8 0 35 55 40 734.8 0 40 56 41 734.8 0 41 57 39 734.8 0 39 58 57 734.8 0 57 59 31 734.8 0 31 60 38 734.8 0 38 61 45 734.8 0 45 62 26 734.8 0 26 63 44 734.8 0 44 64 50 734.8 0 50 65 50 734.8 0 50 66 43 734.8 0 43 67 53 734.8 0 53 68 45 734.8 0 45 69 29 734.8 0 29 70 40 734.8 0 40 71 58 734.8 0 58 72 37 734.8 0 37 73 43 734.8 0 43 74 49 734.8 0 49 75 49 734.8 0 49 76 200 734.8 0 200 77 102 734.8 0 102 78 50 734.8 0 50 79 51 734.8 0 51 80 44 734.8 0 44 81 43 734.8 0 43 82 48 734.8 0 48 83 60 734.8 0 60 84 64 734.8 0 64 85 44 734.8 0 44 86 43 734.8 0 43 87 50 734.8 0 50 88 53 734.8 0 53 89 36 734.8 0 36 90 45 734.8 0 45 91 62 734.8 0 62 92 73 734.8 0 73 93 249 734.8 0 249 94 170 734.8 0 170 95 65 734.8 0 65 96 192 734.8 0 192 97 253 734.8 0 253 98 129 734.8 0 129 99 58 734.8 0 58 100 43 734.8 0 43 101 54 734.8 0 54 102 41 734.8 0 41 103 53 734.8 0 53 104 30 734.8 0 30 105 39 734.8 0 39 106 40 734.8 0 40 107 44 734.8 0 44 108 45 734.8 0 45 109 55 734.8 0 55 110 48 734.8 0 48 111 33 734.8 0 33 112 31 734.8 0 31 113 37 734.8 0 37 114 77 734.8 0 77 115 69 734.8 0 69 116 77 734.8 0 77 117 113 734.8 0 113 118 62 734.8 0 62 119 67 734.8 0 67 120 83 734.8 0 83 121 52 734.8 0 52 122 59 734.8 0 59 123 108 734.8 0 108 124 72 734.8 0 72 125 114 734.8 0 114 126 121 734.8 0 121 127 204 734.8 0 204 128 230 734.8 0 230 129 543 734.8 0 543 130 12055 734.8 0 12055 131 4405 734.8 0 4405 132 569 734.8 0 569 133 167 734.8 0 167 134 43 734.8 0 43 135 35 734.8 0 35 136 40 734.8 0 40 137 40 734.8 0 40 138 48 734.8 0 48 139 33 734.8 0 33 140 37 734.8 0 37 141 37 734.8 0 37 142 38 734.8 0 38 143 69 734.8 0 69 144 84 734.8 0 84 145 38 734.8 0 38 146 28 734.8 0 28 147 29 734.8 0 29 148 41 734.8 0 41 149 43 734.8 0 43 150 38 734.8 0 38 151 39 734.8 0 39 152 48 734.8 0 48 153 39 734.8 0 39 154 49 734.8 0 49 155 32 734.8 0 32 156 37 734.8 0 37 157 40 734.8 0 40 158 47 734.8 0 47 159 51 734.8 0 51 160 40 734.8 0 40 161 54 734.8 0 54 162 62 734.8 0 62 163 34 734.8 0 34 164 46 734.8 0 46 165 64 734.8 0 64 166 60 734.8 0 60 167 115 734.8 0 115 168 147 734.8 0 147 169 2279 734.8 0 2279 170 1607 734.8 0 1607 171 428 734.8 0 428 172 35 734.8 0 35 173 37 734.8 0 37 174 55 734.8 0 55 175 29 734.8 0 29 176 31 734.8 0 31 177 20 734.8 0 20 178 24 734.8 0 24 179 48 734.8 0 48 180 45 734.8 0 45 181 37 734.8 0 37 182 34 734.8 0 34 183 24 734.8 0 24 184 44 734.8 0 44 185 47 734.8 0 47 186 40 734.8 0 40 187 28 734.8 0 28 188 31 734.8 0 31 189 43 734.8 0 43 190 43 734.8 0 43 191 49 734.8 0 49 192 66 734.8 0 66 193 59 734.8 0 59 194 34 734.8 0 34 195 26 734.8 0 26 196 28 734.8 0 28 197 40 734.8 0 40 198 35 734.8 0 35 199 25 734.8 0 25 200 31 734.8 0 31 201 38 734.8 0 38 202 38 734.8 0 38 203 84 734.8 0 84 204 78 734.8 0 78 205 53 734.8 0 53 206 41 734.8 0 41 207 31 734.8 0 31 208 35 734.8 0 35 209 59 734.8 0 59 210 58 734.8 0 58 211 60 734.8 0 60 212 52 734.8 0 52 213 34 734.8 0 34 214 42 734.8 0 42 215 71 734.8 0 71 216 50 734.8 0 50 217 43 734.8 0 43 218 47 734.8 0 47 219 35 734.8 0 35 220 42 734.8 0 42 221 43 734.8 0 43 222 143 734.8 0 143 223 137 734.8 0 137 224 57 734.8 0 57 225 42 734.8 0 42 226 52 734.8 0 52 227 39 734.8 0 39 228 45 734.8 0 45 229 33 734.8 0 33 230 39 734.8 0 39 231 62 734.8 0 62 232 97 734.8 0 97 233 62 734.8 0 62 234 51 734.8 0 51 235 95 734.8 0 95 236 93 734.8 0 93 237 376 734.8 0 376 238 52 734.8 0 52 239 42 734.8 0 42 240 30 734.8 0 30 241 46 734.8 0 46 242 46 734.8 0 46 243 26 734.8 0 26 244 30 734.8 0 30 245 34 734.8 0 34 246 52 734.8 0 52 247 32 734.8 0 32 248 21 734.8 0 21 249 33 734.8 0 33 250 37 734.8 0 37 251 45 734.8 0 45 252 32 734.8 0 32 253 69 734.8 0 69 254 54 734.8 0 54 255 53 734.8 0 53 256 66 734.8 0 66 257 57 734.8 0 57 258 33 734.8 0 33 259 26 734.8 0 26 260 31 734.8 0 31 261 43 734.8 0 43 262 47 734.8 0 47 263 34 734.8 0 34 264 50 734.8 0 50 265 26 734.8 0 26 266 49 734.8 0 49 267 26 734.8 0 26 268 37 734.8 0 37 269 36 734.8 0 36 270 45 734.8 0 45 271 127 734.8 0 127 272 164 734.8 0 164 273 141 734.8 0 141 274 62 734.8 0 62 275 23 734.8 0 23 276 43 734.8 0 43 277 37 734.8 0 37 278 25 734.8 0 25 279 33 734.8 0 33 280 30 734.8 0 30 281 28 734.8 0 28 282 29 734.8 0 29 283 33 734.8 0 33 284 34 734.8 0 34 285 27 734.8 0 27 286 36 734.8 0 36 287 34 734.8 0 34 288 33 734.8 0 33 289 36 734.8 0 36 290 34 734.8 0 34 291 44 734.8 0 44 292 34 734.8 0 34 293 52 734.8 0 52 294 29 734.8 0 29 295 25 734.8 0 25 296 102 734.8 0 102 297 123 734.8 0 123 298 29 734.8 0 29 299 23 734.8 0 23 300 21 734.8 0 21 301 24 734.8 0 24 Finished in 12.21 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 282,593 Reads with adapters: 3,450 (1.2%) Reads that were too short: 162 (0.1%) Reads written (passing filters): 3,288 (1.2%) Total basepairs processed: 78,960,053 bp Total written (filtered): 899,151 bp (1.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3450 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 39 0.0 3 0 38 0 1 17 3163 0.0 3 3089 68 6 18 7 0.0 3 0 5 0 2 19 2 0.0 3 0 0 1 1 20 4 0.0 3 0 0 0 4 22 2 0.0 3 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 62 5 0.0 3 0 0 0 5 63 1 0.0 3 0 0 0 1 65 6 0.0 3 0 0 0 6 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 1 102 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 40 0.0 3 0 0 0 40 193 1 0.0 3 0 0 0 1 194 10 0.0 3 0 0 0 10 205 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 3 0.0 3 0 0 1 2 269 1 0.0 3 0 0 0 1 270 5 0.0 3 0 0 0 5 271 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 6 0.0 3 0 0 0 6 281 31 0.0 3 0 0 0 31 282 47 0.0 3 0 0 0 47 283 20 0.0 3 0 0 1 19 285 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 288 16 0.0 3 0 0 0 16 289 2 0.0 3 0 0 0 2 290 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (36 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 3,288 Reads with adapters: 197 (6.0%) Reads written (passing filters): 3,288 (100.0%) Total basepairs processed: 899,151 bp Total written (filtered): 898,554 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 197 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.1% C: 34.5% G: 24.9% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 193 51.4 0 193 4 2 12.8 0 2 5 2 3.2 0 2