This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.02 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 137,445 (4.6%) Reads that were too short: 5,924 (0.2%) Reads written (passing filters): 131,521 (4.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 30,865,218 bp (3.4%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 137445 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1956 734.8 0 1956 7 56356 734.8 0 56356 8 249 734.8 0 249 9 8851 734.8 0 8851 10 59 734.8 0 59 11 39 734.8 0 39 12 35 734.8 0 35 13 128 734.8 0 128 14 37 734.8 0 37 15 42 734.8 0 42 16 44 734.8 0 44 17 38 734.8 0 38 18 53 734.8 0 53 19 55 734.8 0 55 20 35 734.8 0 35 21 58 734.8 0 58 22 36 734.8 0 36 23 66 734.8 0 66 24 60 734.8 0 60 25 50 734.8 0 50 26 53 734.8 0 53 27 62 734.8 0 62 28 46 734.8 0 46 29 33 734.8 0 33 30 63 734.8 0 63 31 64 734.8 0 64 32 80 734.8 0 80 33 64 734.8 0 64 34 132 734.8 0 132 35 58 734.8 0 58 36 76 734.8 0 76 37 489 734.8 0 489 38 434 734.8 0 434 39 345 734.8 0 345 40 63 734.8 0 63 41 40 734.8 0 40 42 64 734.8 0 64 43 52 734.8 0 52 44 51 734.8 0 51 45 55 734.8 0 55 46 47 734.8 0 47 47 64 734.8 0 64 48 50 734.8 0 50 49 45 734.8 0 45 50 40 734.8 0 40 51 56 734.8 0 56 52 56 734.8 0 56 53 27 734.8 0 27 54 45 734.8 0 45 55 46 734.8 0 46 56 42 734.8 0 42 57 47 734.8 0 47 58 45 734.8 0 45 59 63 734.8 0 63 60 40 734.8 0 40 61 42 734.8 0 42 62 132 734.8 0 132 63 67 734.8 0 67 64 35 734.8 0 35 65 48 734.8 0 48 66 37 734.8 0 37 67 46 734.8 0 46 68 42 734.8 0 42 69 38 734.8 0 38 70 56 734.8 0 56 71 49 734.8 0 49 72 38 734.8 0 38 73 57 734.8 0 57 74 34 734.8 0 34 75 101 734.8 0 101 76 80 734.8 0 80 77 50 734.8 0 50 78 58 734.8 0 58 79 50 734.8 0 50 80 44 734.8 0 44 81 49 734.8 0 49 82 48 734.8 0 48 83 50 734.8 0 50 84 43 734.8 0 43 85 40 734.8 0 40 86 57 734.8 0 57 87 36 734.8 0 36 88 59 734.8 0 59 89 48 734.8 0 48 90 90 734.8 0 90 91 116 734.8 0 116 92 2026 734.8 0 2026 93 111 734.8 0 111 94 45 734.8 0 45 95 51 734.8 0 51 96 44 734.8 0 44 97 52 734.8 0 52 98 43 734.8 0 43 99 33 734.8 0 33 100 44 734.8 0 44 101 40 734.8 0 40 102 48 734.8 0 48 103 40 734.8 0 40 104 71 734.8 0 71 105 48 734.8 0 48 106 35 734.8 0 35 107 52 734.8 0 52 108 52 734.8 0 52 109 57 734.8 0 57 110 44 734.8 0 44 111 40 734.8 0 40 112 77 734.8 0 77 113 182 734.8 0 182 114 67 734.8 0 67 115 138 734.8 0 138 116 109 734.8 0 109 117 53 734.8 0 53 118 104 734.8 0 104 119 82 734.8 0 82 120 79 734.8 0 79 121 87 734.8 0 87 122 108 734.8 0 108 123 91 734.8 0 91 124 104 734.8 0 104 125 140 734.8 0 140 126 253 734.8 0 253 127 219 734.8 0 219 128 532 734.8 0 532 129 13186 734.8 0 13186 130 4242 734.8 0 4242 131 534 734.8 0 534 132 156 734.8 0 156 133 107 734.8 0 107 134 125 734.8 0 125 135 143 734.8 0 143 136 143 734.8 0 143 137 204 734.8 0 204 138 207 734.8 0 207 139 230 734.8 0 230 140 287 734.8 0 287 141 591 734.8 0 591 142 13872 734.8 0 13872 143 14754 734.8 0 14754 144 1888 734.8 0 1888 145 46 734.8 0 46 146 45 734.8 0 45 147 29 734.8 0 29 148 41 734.8 0 41 149 202 734.8 0 202 150 155 734.8 0 155 151 56 734.8 0 56 152 46 734.8 0 46 153 48 734.8 0 48 154 44 734.8 0 44 155 31 734.8 0 31 156 42 734.8 0 42 157 48 734.8 0 48 158 39 734.8 0 39 159 36 734.8 0 36 160 20 734.8 0 20 161 28 734.8 0 28 162 29 734.8 0 29 163 48 734.8 0 48 164 54 734.8 0 54 165 61 734.8 0 61 166 43 734.8 0 43 167 31 734.8 0 31 168 42 734.8 0 42 169 81 734.8 0 81 170 41 734.8 0 41 171 28 734.8 0 28 172 40 734.8 0 40 173 39 734.8 0 39 174 35 734.8 0 35 175 33 734.8 0 33 176 47 734.8 0 47 177 34 734.8 0 34 178 35 734.8 0 35 179 34 734.8 0 34 180 20 734.8 0 20 181 31 734.8 0 31 182 49 734.8 0 49 183 34 734.8 0 34 184 32 734.8 0 32 185 35 734.8 0 35 186 42 734.8 0 42 187 24 734.8 0 24 188 32 734.8 0 32 189 40 734.8 0 40 190 38 734.8 0 38 191 45 734.8 0 45 192 34 734.8 0 34 193 32 734.8 0 32 194 25 734.8 0 25 195 26 734.8 0 26 196 24 734.8 0 24 197 34 734.8 0 34 198 22 734.8 0 22 199 26 734.8 0 26 200 33 734.8 0 33 201 38 734.8 0 38 202 29 734.8 0 29 203 46 734.8 0 46 204 54 734.8 0 54 205 40 734.8 0 40 206 23 734.8 0 23 207 27 734.8 0 27 208 33 734.8 0 33 209 39 734.8 0 39 210 73 734.8 0 73 211 28 734.8 0 28 212 40 734.8 0 40 213 41 734.8 0 41 214 200 734.8 0 200 215 188 734.8 0 188 216 116 734.8 0 116 217 67 734.8 0 67 218 31 734.8 0 31 219 35 734.8 0 35 220 33 734.8 0 33 221 33 734.8 0 33 222 38 734.8 0 38 223 26 734.8 0 26 224 47 734.8 0 47 225 24 734.8 0 24 226 39 734.8 0 39 227 49 734.8 0 49 228 41 734.8 0 41 229 48 734.8 0 48 230 112 734.8 0 112 231 48 734.8 0 48 232 46 734.8 0 46 233 52 734.8 0 52 234 686 734.8 0 686 235 598 734.8 0 598 236 443 734.8 0 443 237 65 734.8 0 65 238 34 734.8 0 34 239 27 734.8 0 27 240 42 734.8 0 42 241 25 734.8 0 25 242 24 734.8 0 24 243 35 734.8 0 35 244 41 734.8 0 41 245 36 734.8 0 36 246 29 734.8 0 29 247 30 734.8 0 30 248 26 734.8 0 26 249 26 734.8 0 26 250 26 734.8 0 26 251 27 734.8 0 27 252 29 734.8 0 29 253 39 734.8 0 39 254 68 734.8 0 68 255 59 734.8 0 59 256 50 734.8 0 50 257 26 734.8 0 26 258 26 734.8 0 26 259 28 734.8 0 28 260 33 734.8 0 33 261 57 734.8 0 57 262 42 734.8 0 42 263 117 734.8 0 117 264 37 734.8 0 37 265 48 734.8 0 48 266 36 734.8 0 36 267 26 734.8 0 26 268 34 734.8 0 34 269 33 734.8 0 33 270 62 734.8 0 62 271 40 734.8 0 40 272 26 734.8 0 26 273 34 734.8 0 34 274 34 734.8 0 34 275 30 734.8 0 30 276 44 734.8 0 44 277 59 734.8 0 59 278 39 734.8 0 39 279 26 734.8 0 26 280 27 734.8 0 27 281 39 734.8 0 39 282 26 734.8 0 26 283 36 734.8 0 36 284 34 734.8 0 34 285 39 734.8 0 39 286 20 734.8 0 20 287 23 734.8 0 23 288 28 734.8 0 28 289 26 734.8 0 26 290 21 734.8 0 21 291 36 734.8 0 36 292 35 734.8 0 35 293 30 734.8 0 30 294 23 734.8 0 23 295 36 734.8 0 36 296 61 734.8 0 61 297 66 734.8 0 66 298 77 734.8 0 77 299 33 734.8 0 33 300 23 734.8 0 23 301 37 734.8 0 37 Finished in 5.64 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 131,521 Reads with adapters: 66,116 (50.3%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 66,112 (50.3%) Total basepairs processed: 30,865,218 bp Total written (filtered): 18,036,274 bp (58.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 66116 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 7 0.0 3 0 0 3 2 2 19 120 0.0 3 0 87 28 3 2 20 9475 0.0 4 8786 670 14 1 4 21 56196 0.0 4 55471 700 24 0 1 22 177 0.0 4 51 119 7 23 52 0.0 4 42 1 6 2 1 24 28 0.0 4 2 0 0 21 5 25 5 0.0 4 0 0 0 0 5 27 1 0.0 4 1 42 9 0.0 4 0 0 0 0 9 97 9 0.0 4 0 0 0 0 9 111 17 0.0 4 0 0 0 16 1 112 7 0.0 4 0 0 0 7 119 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 128 6 0.0 4 0 0 0 0 6 198 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.25 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 66,112 Reads with adapters: 7,007 (10.6%) Reads written (passing filters): 66,112 (100.0%) Total basepairs processed: 18,036,274 bp Total written (filtered): 16,756,533 bp (92.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7007 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 51.1% C: 8.6% G: 30.0% T: 7.7% none/other: 2.6% Overview of removed sequences length count expect max.err error counts 3 6 1033.0 0 6 4 5 258.2 0 5 5 3 64.6 0 3 6 1 16.1 0 1 9 12 0.3 0 0 12 10 450 0.1 1 12 438 11 1691 0.0 1 4 1687 13 1 0.0 1 0 1 14 1 0.0 1 0 1 23 2 0.0 1 2 151 1 0.0 1 0 1 152 1 0.0 1 0 1 224 1 0.0 1 1 228 1 0.0 1 1 253 1 0.0 1 1 254 29 0.0 1 27 2 255 3 0.0 1 3 256 60 0.0 1 58 2 257 3313 0.0 1 3078 235 258 74 0.0 1 71 3 259 49 0.0 1 49 260 25 0.0 1 24 1 261 1 0.0 1 1 262 3 0.0 1 3 263 114 0.0 1 107 7 264 1 0.0 1 1 265 1 0.0 1 1 266 2 0.0 1 2 267 402 0.0 1 386 16 268 546 0.0 1 520 26 269 13 0.0 1 13 270 7 0.0 1 7 271 8 0.0 1 8 272 2 0.0 1 2 273 172 0.0 1 165 7 274 5 0.0 1 5