This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.81 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 59,525 (2.0%) Reads that were too short: 3,454 (0.1%) Reads written (passing filters): 56,071 (1.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 15,810,457 bp (1.7%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 59525 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 520 734.8 0 520 7 941 734.8 0 941 8 43514 734.8 0 43514 9 4834 734.8 0 4834 10 27 734.8 0 27 11 124 734.8 0 124 12 18 734.8 0 18 13 21 734.8 0 21 14 12 734.8 0 12 15 17 734.8 0 17 16 21 734.8 0 21 17 8 734.8 0 8 18 14 734.8 0 14 19 16 734.8 0 16 20 16 734.8 0 16 21 25 734.8 0 25 22 22 734.8 0 22 23 21 734.8 0 21 24 9 734.8 0 9 25 22 734.8 0 22 26 12 734.8 0 12 27 22 734.8 0 22 28 15 734.8 0 15 29 12 734.8 0 12 30 10 734.8 0 10 31 11 734.8 0 11 32 14 734.8 0 14 33 19 734.8 0 19 34 23 734.8 0 23 35 28 734.8 0 28 36 29 734.8 0 29 37 16 734.8 0 16 38 28 734.8 0 28 39 19 734.8 0 19 40 21 734.8 0 21 41 29 734.8 0 29 42 11 734.8 0 11 43 16 734.8 0 16 44 37 734.8 0 37 45 9 734.8 0 9 46 25 734.8 0 25 47 682 734.8 0 682 48 235 734.8 0 235 49 200 734.8 0 200 50 128 734.8 0 128 51 11 734.8 0 11 52 19 734.8 0 19 53 17 734.8 0 17 54 19 734.8 0 19 55 18 734.8 0 18 56 9 734.8 0 9 57 13 734.8 0 13 58 9 734.8 0 9 59 15 734.8 0 15 60 15 734.8 0 15 61 13 734.8 0 13 62 11 734.8 0 11 63 5 734.8 0 5 64 10 734.8 0 10 65 7 734.8 0 7 66 9 734.8 0 9 67 13 734.8 0 13 68 4 734.8 0 4 69 14 734.8 0 14 70 5 734.8 0 5 71 16 734.8 0 16 72 8 734.8 0 8 73 5 734.8 0 5 74 8 734.8 0 8 75 15 734.8 0 15 76 7 734.8 0 7 77 11 734.8 0 11 78 23 734.8 0 23 79 14 734.8 0 14 80 23 734.8 0 23 81 23 734.8 0 23 82 20 734.8 0 20 83 42 734.8 0 42 84 39 734.8 0 39 85 27 734.8 0 27 86 18 734.8 0 18 87 53 734.8 0 53 88 37 734.8 0 37 89 27 734.8 0 27 90 31 734.8 0 31 91 14 734.8 0 14 92 12 734.8 0 12 93 18 734.8 0 18 94 15 734.8 0 15 95 30 734.8 0 30 96 261 734.8 0 261 97 35 734.8 0 35 98 18 734.8 0 18 99 9 734.8 0 9 100 8 734.8 0 8 101 15 734.8 0 15 102 25 734.8 0 25 103 28 734.8 0 28 104 30 734.8 0 30 105 14 734.8 0 14 106 6 734.8 0 6 107 62 734.8 0 62 108 15 734.8 0 15 109 20 734.8 0 20 110 11 734.8 0 11 111 19 734.8 0 19 112 17 734.8 0 17 113 47 734.8 0 47 114 27 734.8 0 27 115 38 734.8 0 38 116 23 734.8 0 23 117 19 734.8 0 19 118 121 734.8 0 121 119 73 734.8 0 73 120 20 734.8 0 20 121 17 734.8 0 17 122 27 734.8 0 27 123 17 734.8 0 17 124 23 734.8 0 23 125 18 734.8 0 18 126 23 734.8 0 23 127 27 734.8 0 27 128 15 734.8 0 15 129 29 734.8 0 29 130 9 734.8 0 9 131 13 734.8 0 13 132 26 734.8 0 26 133 26 734.8 0 26 134 22 734.8 0 22 135 16 734.8 0 16 136 106 734.8 0 106 137 54 734.8 0 54 138 23 734.8 0 23 139 12 734.8 0 12 140 28 734.8 0 28 141 18 734.8 0 18 142 22 734.8 0 22 143 30 734.8 0 30 144 14 734.8 0 14 145 14 734.8 0 14 146 32 734.8 0 32 147 50 734.8 0 50 148 31 734.8 0 31 149 22 734.8 0 22 150 16 734.8 0 16 151 10 734.8 0 10 152 20 734.8 0 20 153 14 734.8 0 14 154 16 734.8 0 16 155 15 734.8 0 15 156 23 734.8 0 23 157 13 734.8 0 13 158 16 734.8 0 16 159 14 734.8 0 14 160 13 734.8 0 13 161 16 734.8 0 16 162 13 734.8 0 13 163 17 734.8 0 17 164 16 734.8 0 16 165 12 734.8 0 12 166 12 734.8 0 12 167 9 734.8 0 9 168 25 734.8 0 25 169 20 734.8 0 20 170 22 734.8 0 22 171 22 734.8 0 22 172 11 734.8 0 11 173 15 734.8 0 15 174 17 734.8 0 17 175 14 734.8 0 14 176 26 734.8 0 26 177 15 734.8 0 15 178 15 734.8 0 15 179 16 734.8 0 16 180 9 734.8 0 9 181 23 734.8 0 23 182 59 734.8 0 59 183 20 734.8 0 20 184 14 734.8 0 14 185 21 734.8 0 21 186 18 734.8 0 18 187 18 734.8 0 18 188 15 734.8 0 15 189 24 734.8 0 24 190 14 734.8 0 14 191 35 734.8 0 35 192 34 734.8 0 34 193 57 734.8 0 57 194 60 734.8 0 60 195 325 734.8 0 325 196 309 734.8 0 309 197 115 734.8 0 115 198 31 734.8 0 31 199 20 734.8 0 20 200 12 734.8 0 12 201 5 734.8 0 5 202 13 734.8 0 13 203 17 734.8 0 17 204 15 734.8 0 15 205 14 734.8 0 14 206 12 734.8 0 12 207 17 734.8 0 17 208 11 734.8 0 11 209 15 734.8 0 15 210 15 734.8 0 15 211 14 734.8 0 14 212 23 734.8 0 23 213 35 734.8 0 35 214 47 734.8 0 47 215 55 734.8 0 55 216 31 734.8 0 31 217 13 734.8 0 13 218 40 734.8 0 40 219 40 734.8 0 40 220 16 734.8 0 16 221 22 734.8 0 22 222 15 734.8 0 15 223 18 734.8 0 18 224 13 734.8 0 13 225 32 734.8 0 32 226 14 734.8 0 14 227 12 734.8 0 12 228 21 734.8 0 21 229 50 734.8 0 50 230 44 734.8 0 44 231 29 734.8 0 29 232 23 734.8 0 23 233 23 734.8 0 23 234 28 734.8 0 28 235 59 734.8 0 59 236 27 734.8 0 27 237 32 734.8 0 32 238 61 734.8 0 61 239 80 734.8 0 80 240 51 734.8 0 51 241 17 734.8 0 17 242 22 734.8 0 22 243 78 734.8 0 78 244 137 734.8 0 137 245 101 734.8 0 101 246 171 734.8 0 171 247 97 734.8 0 97 248 60 734.8 0 60 249 14 734.8 0 14 250 14 734.8 0 14 251 51 734.8 0 51 252 49 734.8 0 49 253 25 734.8 0 25 254 21 734.8 0 21 255 44 734.8 0 44 256 56 734.8 0 56 257 66 734.8 0 66 258 37 734.8 0 37 259 27 734.8 0 27 260 38 734.8 0 38 261 41 734.8 0 41 262 18 734.8 0 18 263 12 734.8 0 12 264 7 734.8 0 7 265 22 734.8 0 22 266 33 734.8 0 33 267 23 734.8 0 23 268 81 734.8 0 81 269 47 734.8 0 47 270 19 734.8 0 19 271 12 734.8 0 12 272 10 734.8 0 10 273 21 734.8 0 21 274 28 734.8 0 28 275 27 734.8 0 27 276 15 734.8 0 15 277 11 734.8 0 11 278 9 734.8 0 9 279 75 734.8 0 75 280 76 734.8 0 76 281 35 734.8 0 35 282 24 734.8 0 24 283 15 734.8 0 15 284 15 734.8 0 15 285 36 734.8 0 36 286 34 734.8 0 34 287 45 734.8 0 45 288 36 734.8 0 36 289 24 734.8 0 24 290 23 734.8 0 23 291 31 734.8 0 31 292 44 734.8 0 44 293 44 734.8 0 44 294 39 734.8 0 39 295 38 734.8 0 38 296 41 734.8 0 41 297 26 734.8 0 26 298 14 734.8 0 14 299 23 734.8 0 23 300 30 734.8 0 30 301 28 734.8 0 28 Finished in 2.53 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 56,071 Reads with adapters: 44,079 (78.6%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 44,060 (78.6%) Total basepairs processed: 15,810,457 bp Total written (filtered): 12,157,099 bp (76.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 44079 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 25 0.0 3 0 0 16 9 16 400 0.0 3 0 365 23 12 17 43318 0.0 3 42048 1124 122 24 18 124 0.0 3 10 98 14 2 19 98 0.0 3 12 3 47 36 20 57 0.0 3 1 0 0 56 21 3 0.0 3 0 1 1 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 1 38 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 1 1 93 1 0.0 3 0 0 0 1 95 4 0.0 3 0 0 1 3 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 1 1 137 1 0.0 3 0 0 0 1 143 8 0.0 3 0 0 0 8 146 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.55 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 44,060 Reads with adapters: 728 (1.7%) Reads written (passing filters): 44,060 (100.0%) Total basepairs processed: 12,157,099 bp Total written (filtered): 12,154,892 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 728 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.8% C: 12.1% G: 38.2% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 707 688.4 0 707 4 19 172.1 0 19 5 2 43.0 0 2