This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.04 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 82,192 (2.7%) Reads that were too short: 11,261 (0.4%) Reads written (passing filters): 70,931 (2.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 19,301,163 bp (2.1%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 82192 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10314 734.8 0 10314 7 6447 734.8 0 6447 8 41958 734.8 0 41958 9 83 734.8 0 83 10 19 734.8 0 19 11 15 734.8 0 15 12 20 734.8 0 20 13 20 734.8 0 20 14 30 734.8 0 30 15 22 734.8 0 22 16 19 734.8 0 19 17 17 734.8 0 17 18 36 734.8 0 36 19 23 734.8 0 23 20 24 734.8 0 24 21 31 734.8 0 31 22 28 734.8 0 28 23 12 734.8 0 12 24 12 734.8 0 12 25 17 734.8 0 17 26 19 734.8 0 19 27 22 734.8 0 22 28 15 734.8 0 15 29 19 734.8 0 19 30 18 734.8 0 18 31 9 734.8 0 9 32 13 734.8 0 13 33 20 734.8 0 20 34 24 734.8 0 24 35 16 734.8 0 16 36 15 734.8 0 15 37 22 734.8 0 22 38 22 734.8 0 22 39 16 734.8 0 16 40 28 734.8 0 28 41 16 734.8 0 16 42 19 734.8 0 19 43 17 734.8 0 17 44 19 734.8 0 19 45 17 734.8 0 17 46 40 734.8 0 40 47 16 734.8 0 16 48 19 734.8 0 19 49 28 734.8 0 28 50 16 734.8 0 16 51 20 734.8 0 20 52 47 734.8 0 47 53 23 734.8 0 23 54 38 734.8 0 38 55 55 734.8 0 55 56 38 734.8 0 38 57 14 734.8 0 14 58 15 734.8 0 15 59 25 734.8 0 25 60 28 734.8 0 28 61 64 734.8 0 64 62 64 734.8 0 64 63 36 734.8 0 36 64 25 734.8 0 25 65 19 734.8 0 19 66 23 734.8 0 23 67 48 734.8 0 48 68 29 734.8 0 29 69 27 734.8 0 27 70 14 734.8 0 14 71 28 734.8 0 28 72 32 734.8 0 32 73 19 734.8 0 19 74 33 734.8 0 33 75 16 734.8 0 16 76 40 734.8 0 40 77 63 734.8 0 63 78 69 734.8 0 69 79 43 734.8 0 43 80 53 734.8 0 53 81 39 734.8 0 39 82 24 734.8 0 24 83 31 734.8 0 31 84 24 734.8 0 24 85 30 734.8 0 30 86 24 734.8 0 24 87 20 734.8 0 20 88 19 734.8 0 19 89 26 734.8 0 26 90 30 734.8 0 30 91 36 734.8 0 36 92 51 734.8 0 51 93 98 734.8 0 98 94 103 734.8 0 103 95 73 734.8 0 73 96 50 734.8 0 50 97 45 734.8 0 45 98 117 734.8 0 117 99 232 734.8 0 232 100 445 734.8 0 445 101 43 734.8 0 43 102 31 734.8 0 31 103 17 734.8 0 17 104 16 734.8 0 16 105 23 734.8 0 23 106 18 734.8 0 18 107 34 734.8 0 34 108 54 734.8 0 54 109 42 734.8 0 42 110 44 734.8 0 44 111 42 734.8 0 42 112 72 734.8 0 72 113 49 734.8 0 49 114 34 734.8 0 34 115 52 734.8 0 52 116 40 734.8 0 40 117 40 734.8 0 40 118 51 734.8 0 51 119 45 734.8 0 45 120 43 734.8 0 43 121 46 734.8 0 46 122 60 734.8 0 60 123 94 734.8 0 94 124 69 734.8 0 69 125 76 734.8 0 76 126 195 734.8 0 195 127 124 734.8 0 124 128 381 734.8 0 381 129 466 734.8 0 466 130 345 734.8 0 345 131 105 734.8 0 105 132 68 734.8 0 68 133 58 734.8 0 58 134 77 734.8 0 77 135 102 734.8 0 102 136 92 734.8 0 92 137 64 734.8 0 64 138 67 734.8 0 67 139 66 734.8 0 66 140 48 734.8 0 48 141 62 734.8 0 62 142 60 734.8 0 60 143 49 734.8 0 49 144 61 734.8 0 61 145 78 734.8 0 78 146 73 734.8 0 73 147 62 734.8 0 62 148 57 734.8 0 57 149 56 734.8 0 56 150 49 734.8 0 49 151 51 734.8 0 51 152 49 734.8 0 49 153 51 734.8 0 51 154 72 734.8 0 72 155 78 734.8 0 78 156 71 734.8 0 71 157 97 734.8 0 97 158 45 734.8 0 45 159 65 734.8 0 65 160 56 734.8 0 56 161 43 734.8 0 43 162 69 734.8 0 69 163 55 734.8 0 55 164 64 734.8 0 64 165 83 734.8 0 83 166 186 734.8 0 186 167 173 734.8 0 173 168 97 734.8 0 97 169 58 734.8 0 58 170 47 734.8 0 47 171 58 734.8 0 58 172 67 734.8 0 67 173 41 734.8 0 41 174 111 734.8 0 111 175 95 734.8 0 95 176 75 734.8 0 75 177 67 734.8 0 67 178 57 734.8 0 57 179 70 734.8 0 70 180 69 734.8 0 69 181 80 734.8 0 80 182 81 734.8 0 81 183 59 734.8 0 59 184 86 734.8 0 86 185 103 734.8 0 103 186 83 734.8 0 83 187 59 734.8 0 59 188 51 734.8 0 51 189 80 734.8 0 80 190 103 734.8 0 103 191 138 734.8 0 138 192 148 734.8 0 148 193 113 734.8 0 113 194 100 734.8 0 100 195 149 734.8 0 149 196 153 734.8 0 153 197 412 734.8 0 412 198 276 734.8 0 276 199 121 734.8 0 121 200 60 734.8 0 60 201 59 734.8 0 59 202 110 734.8 0 110 203 99 734.8 0 99 204 100 734.8 0 100 205 86 734.8 0 86 206 66 734.8 0 66 207 96 734.8 0 96 208 68 734.8 0 68 209 75 734.8 0 75 210 116 734.8 0 116 211 246 734.8 0 246 212 200 734.8 0 200 213 124 734.8 0 124 214 95 734.8 0 95 215 96 734.8 0 96 216 97 734.8 0 97 217 96 734.8 0 96 218 184 734.8 0 184 219 166 734.8 0 166 220 180 734.8 0 180 221 161 734.8 0 161 222 132 734.8 0 132 223 171 734.8 0 171 224 118 734.8 0 118 225 105 734.8 0 105 226 141 734.8 0 141 227 83 734.8 0 83 228 77 734.8 0 77 229 81 734.8 0 81 230 67 734.8 0 67 231 74 734.8 0 74 232 76 734.8 0 76 233 100 734.8 0 100 234 113 734.8 0 113 235 145 734.8 0 145 236 144 734.8 0 144 237 113 734.8 0 113 238 213 734.8 0 213 239 161 734.8 0 161 240 154 734.8 0 154 241 159 734.8 0 159 242 180 734.8 0 180 243 139 734.8 0 139 244 121 734.8 0 121 245 141 734.8 0 141 246 177 734.8 0 177 247 84 734.8 0 84 248 31 734.8 0 31 249 48 734.8 0 48 250 92 734.8 0 92 251 171 734.8 0 171 252 236 734.8 0 236 253 183 734.8 0 183 254 108 734.8 0 108 255 225 734.8 0 225 256 102 734.8 0 102 257 59 734.8 0 59 258 55 734.8 0 55 259 67 734.8 0 67 260 68 734.8 0 68 261 103 734.8 0 103 262 308 734.8 0 308 263 237 734.8 0 237 264 209 734.8 0 209 265 99 734.8 0 99 266 59 734.8 0 59 267 70 734.8 0 70 268 58 734.8 0 58 269 46 734.8 0 46 270 53 734.8 0 53 271 102 734.8 0 102 272 197 734.8 0 197 273 153 734.8 0 153 274 128 734.8 0 128 275 91 734.8 0 91 276 84 734.8 0 84 277 83 734.8 0 83 278 116 734.8 0 116 279 90 734.8 0 90 280 67 734.8 0 67 281 48 734.8 0 48 282 72 734.8 0 72 283 101 734.8 0 101 284 107 734.8 0 107 285 72 734.8 0 72 286 73 734.8 0 73 287 48 734.8 0 48 288 37 734.8 0 37 289 43 734.8 0 43 290 92 734.8 0 92 291 167 734.8 0 167 292 101 734.8 0 101 293 46 734.8 0 46 294 29 734.8 0 29 295 25 734.8 0 25 296 35 734.8 0 35 297 85 734.8 0 85 298 83 734.8 0 83 299 160 734.8 0 160 300 222 734.8 0 222 301 87 734.8 0 87 Finished in 3.46 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 70,931 Reads with adapters: 42,715 (60.2%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 42,710 (60.2%) Total basepairs processed: 19,301,163 bp Total written (filtered): 11,655,717 bp (60.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42715 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 23 0.0 3 0 0 22 1 19 525 0.0 3 0 520 2 3 20 41966 0.0 4 41474 475 8 3 6 21 110 0.0 4 17 91 0 0 2 22 13 0.0 4 2 0 10 0 1 23 16 0.0 4 0 0 0 14 2 24 3 0.0 4 0 1 1 1 25 1 0.0 4 1 32 3 0.0 4 0 0 0 0 3 42 1 0.0 4 1 58 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 111 6 0.0 4 0 0 0 4 2 112 2 0.0 4 0 0 0 1 1 114 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 27 0.0 4 0 0 0 0 27 128 8 0.0 4 0 0 0 0 8 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.45 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 42,710 Reads with adapters: 1,909 (4.5%) Reads written (passing filters): 42,710 (100.0%) Total basepairs processed: 11,655,717 bp Total written (filtered): 11,632,474 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1909 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 1.0% G: 97.5% T: 0.7% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 667.3 0 1 4 4 166.8 0 4 5 1 41.7 0 1 6 1 10.4 0 1 10 138 0.0 1 5 133 11 1748 0.0 1 5 1743 14 2 0.0 1 0 2 151 9 0.0 1 0 9 170 1 0.0 1 1 258 2 0.0 1 2 259 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.