This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.34 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 60,586 (2.0%) Reads that were too short: 15,994 (0.5%) Reads written (passing filters): 44,592 (1.5%) Total basepairs processed: 905,907,359 bp Total written (filtered): 11,718,440 bp (1.3%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 60586 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1079 734.8 0 1079 7 1873 734.8 0 1873 8 28480 734.8 0 28480 9 79 734.8 0 79 10 51 734.8 0 51 11 34 734.8 0 34 12 25 734.8 0 25 13 26 734.8 0 26 14 39 734.8 0 39 15 30 734.8 0 30 16 29 734.8 0 29 17 174 734.8 0 174 18 62 734.8 0 62 19 55 734.8 0 55 20 26 734.8 0 26 21 29 734.8 0 29 22 22 734.8 0 22 23 24 734.8 0 24 24 26 734.8 0 26 25 30 734.8 0 30 26 33 734.8 0 33 27 19 734.8 0 19 28 26 734.8 0 26 29 44 734.8 0 44 30 41 734.8 0 41 31 22 734.8 0 22 32 25 734.8 0 25 33 42 734.8 0 42 34 24 734.8 0 24 35 36 734.8 0 36 36 38 734.8 0 38 37 25 734.8 0 25 38 32 734.8 0 32 39 31 734.8 0 31 40 36 734.8 0 36 41 33 734.8 0 33 42 54 734.8 0 54 43 42 734.8 0 42 44 21 734.8 0 21 45 23 734.8 0 23 46 40 734.8 0 40 47 43 734.8 0 43 48 41 734.8 0 41 49 48 734.8 0 48 50 22 734.8 0 22 51 22 734.8 0 22 52 28 734.8 0 28 53 59 734.8 0 59 54 28 734.8 0 28 55 43 734.8 0 43 56 42 734.8 0 42 57 48 734.8 0 48 58 30 734.8 0 30 59 34 734.8 0 34 60 43 734.8 0 43 61 39 734.8 0 39 62 37 734.8 0 37 63 27 734.8 0 27 64 27 734.8 0 27 65 32 734.8 0 32 66 38 734.8 0 38 67 48 734.8 0 48 68 36 734.8 0 36 69 19 734.8 0 19 70 31 734.8 0 31 71 46 734.8 0 46 72 49 734.8 0 49 73 453 734.8 0 453 74 438 734.8 0 438 75 80 734.8 0 80 76 51 734.8 0 51 77 916 734.8 0 916 78 752 734.8 0 752 79 197 734.8 0 197 80 34 734.8 0 34 81 26 734.8 0 26 82 35 734.8 0 35 83 25 734.8 0 25 84 29 734.8 0 29 85 34 734.8 0 34 86 38 734.8 0 38 87 28 734.8 0 28 88 28 734.8 0 28 89 32 734.8 0 32 90 33 734.8 0 33 91 31 734.8 0 31 92 39 734.8 0 39 93 40 734.8 0 40 94 28 734.8 0 28 95 18 734.8 0 18 96 40 734.8 0 40 97 23 734.8 0 23 98 31 734.8 0 31 99 39 734.8 0 39 100 26 734.8 0 26 101 20 734.8 0 20 102 16 734.8 0 16 103 25 734.8 0 25 104 23 734.8 0 23 105 28 734.8 0 28 106 121 734.8 0 121 107 163 734.8 0 163 108 47 734.8 0 47 109 28 734.8 0 28 110 35 734.8 0 35 111 31 734.8 0 31 112 35 734.8 0 35 113 36 734.8 0 36 114 51 734.8 0 51 115 46 734.8 0 46 116 33 734.8 0 33 117 31 734.8 0 31 118 31 734.8 0 31 119 36 734.8 0 36 120 43 734.8 0 43 121 34 734.8 0 34 122 28 734.8 0 28 123 31 734.8 0 31 124 25 734.8 0 25 125 42 734.8 0 42 126 44 734.8 0 44 127 432 734.8 0 432 128 93 734.8 0 93 129 143 734.8 0 143 130 55 734.8 0 55 131 43 734.8 0 43 132 57 734.8 0 57 133 48 734.8 0 48 134 125 734.8 0 125 135 58 734.8 0 58 136 68 734.8 0 68 137 49 734.8 0 49 138 40 734.8 0 40 139 78 734.8 0 78 140 58 734.8 0 58 141 46 734.8 0 46 142 38 734.8 0 38 143 44 734.8 0 44 144 74 734.8 0 74 145 65 734.8 0 65 146 912 734.8 0 912 147 684 734.8 0 684 148 123 734.8 0 123 149 38 734.8 0 38 150 31 734.8 0 31 151 19 734.8 0 19 152 32 734.8 0 32 153 31 734.8 0 31 154 28 734.8 0 28 155 64 734.8 0 64 156 116 734.8 0 116 157 28 734.8 0 28 158 32 734.8 0 32 159 53 734.8 0 53 160 85 734.8 0 85 161 31 734.8 0 31 162 32 734.8 0 32 163 33 734.8 0 33 164 81 734.8 0 81 165 110 734.8 0 110 166 85 734.8 0 85 167 65 734.8 0 65 168 66 734.8 0 66 169 39 734.8 0 39 170 51 734.8 0 51 171 29 734.8 0 29 172 39 734.8 0 39 173 27 734.8 0 27 174 35 734.8 0 35 175 28 734.8 0 28 176 23 734.8 0 23 177 29 734.8 0 29 178 27 734.8 0 27 179 33 734.8 0 33 180 46 734.8 0 46 181 39 734.8 0 39 182 28 734.8 0 28 183 28 734.8 0 28 184 171 734.8 0 171 185 162 734.8 0 162 186 76 734.8 0 76 187 19 734.8 0 19 188 29 734.8 0 29 189 21 734.8 0 21 190 29 734.8 0 29 191 35 734.8 0 35 192 39 734.8 0 39 193 38 734.8 0 38 194 38 734.8 0 38 195 185 734.8 0 185 196 151 734.8 0 151 197 89 734.8 0 89 198 44 734.8 0 44 199 29 734.8 0 29 200 48 734.8 0 48 201 28 734.8 0 28 202 39 734.8 0 39 203 29 734.8 0 29 204 145 734.8 0 145 205 59 734.8 0 59 206 45 734.8 0 45 207 34 734.8 0 34 208 42 734.8 0 42 209 38 734.8 0 38 210 25 734.8 0 25 211 31 734.8 0 31 212 32 734.8 0 32 213 45 734.8 0 45 214 50 734.8 0 50 215 57 734.8 0 57 216 91 734.8 0 91 217 72 734.8 0 72 218 39 734.8 0 39 219 60 734.8 0 60 220 45 734.8 0 45 221 39 734.8 0 39 222 37 734.8 0 37 223 30 734.8 0 30 224 39 734.8 0 39 225 48 734.8 0 48 226 68 734.8 0 68 227 27 734.8 0 27 228 34 734.8 0 34 229 52 734.8 0 52 230 42 734.8 0 42 231 61 734.8 0 61 232 62 734.8 0 62 233 110 734.8 0 110 234 122 734.8 0 122 235 216 734.8 0 216 236 1700 734.8 0 1700 237 6950 734.8 0 6950 238 660 734.8 0 660 239 49 734.8 0 49 240 28 734.8 0 28 241 41 734.8 0 41 242 28 734.8 0 28 243 18 734.8 0 18 244 44 734.8 0 44 245 41 734.8 0 41 246 86 734.8 0 86 247 70 734.8 0 70 248 35 734.8 0 35 249 30 734.8 0 30 250 21 734.8 0 21 251 21 734.8 0 21 252 81 734.8 0 81 253 81 734.8 0 81 254 121 734.8 0 121 255 64 734.8 0 64 256 49 734.8 0 49 257 33 734.8 0 33 258 28 734.8 0 28 259 25 734.8 0 25 260 20 734.8 0 20 261 18 734.8 0 18 262 15 734.8 0 15 263 23 734.8 0 23 264 34 734.8 0 34 265 35 734.8 0 35 266 42 734.8 0 42 267 39 734.8 0 39 268 32 734.8 0 32 269 25 734.8 0 25 270 28 734.8 0 28 271 29 734.8 0 29 272 41 734.8 0 41 273 36 734.8 0 36 274 33 734.8 0 33 275 24 734.8 0 24 276 72 734.8 0 72 277 131 734.8 0 131 278 224 734.8 0 224 279 177 734.8 0 177 280 196 734.8 0 196 281 671 734.8 0 671 282 332 734.8 0 332 283 79 734.8 0 79 284 242 734.8 0 242 285 200 734.8 0 200 286 67 734.8 0 67 287 20 734.8 0 20 288 28 734.8 0 28 289 26 734.8 0 26 290 23 734.8 0 23 291 28 734.8 0 28 292 40 734.8 0 40 293 54 734.8 0 54 294 39 734.8 0 39 295 41 734.8 0 41 296 151 734.8 0 151 297 205 734.8 0 205 298 93 734.8 0 93 299 27 734.8 0 27 300 52 734.8 0 52 301 33 734.8 0 33 Finished in 1.98 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 44,592 Reads with adapters: 1,619 (3.6%) Reads that were too short: 44 (0.1%) Reads written (passing filters): 1,575 (3.5%) Total basepairs processed: 11,718,440 bp Total written (filtered): 416,543 bp (3.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1619 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 16 0.0 3 0 15 0 1 17 1427 0.0 3 1400 21 4 2 18 5 0.0 3 1 3 0 1 19 1 0.0 3 0 1 20 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 39 7 0.0 3 0 0 0 7 45 12 0.0 3 0 0 1 11 47 1 0.0 3 0 0 0 1 48 5 0.0 3 0 0 0 5 53 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 50 0.0 3 0 0 0 50 115 5 0.0 3 0 0 0 5 117 8 0.0 3 0 0 0 8 118 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 1 2 147 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 192 11 0.0 3 0 0 0 11 194 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 2 1 282 7 0.0 3 0 0 0 7 283 5 0.0 3 0 0 0 5 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 1,575 Reads with adapters: 100 (6.3%) Reads written (passing filters): 1,575 (100.0%) Total basepairs processed: 416,543 bp Total written (filtered): 416,236 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 100 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.0% C: 46.0% G: 13.0% T: 7.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 98 24.6 0 98 5 1 1.5 0 1 8 1 0.0 0 1