This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 80.03 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 58,404 (1.9%) Reads that were too short: 32,994 (1.1%) Reads written (passing filters): 25,410 (0.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 4,406,488 bp (0.5%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 58404 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 250 734.8 0 250 7 1567 734.8 0 1567 8 28 734.8 0 28 9 1490 734.8 0 1490 10 5 734.8 0 5 11 14 734.8 0 14 12 8 734.8 0 8 13 9 734.8 0 9 14 14 734.8 0 14 15 412 734.8 0 412 16 85 734.8 0 85 17 10 734.8 0 10 18 10 734.8 0 10 19 8 734.8 0 8 20 9 734.8 0 9 21 13 734.8 0 13 22 18 734.8 0 18 23 11 734.8 0 11 24 9 734.8 0 9 25 6 734.8 0 6 26 6 734.8 0 6 27 6 734.8 0 6 28 12 734.8 0 12 29 16 734.8 0 16 30 16 734.8 0 16 31 13 734.8 0 13 32 17 734.8 0 17 33 10 734.8 0 10 34 8 734.8 0 8 35 19 734.8 0 19 36 12 734.8 0 12 37 10 734.8 0 10 38 13 734.8 0 13 39 15 734.8 0 15 40 140 734.8 0 140 41 20 734.8 0 20 42 19 734.8 0 19 43 4 734.8 0 4 44 14 734.8 0 14 45 16 734.8 0 16 46 11 734.8 0 11 47 15 734.8 0 15 48 9 734.8 0 9 49 5 734.8 0 5 50 14 734.8 0 14 51 14 734.8 0 14 52 11 734.8 0 11 53 10 734.8 0 10 54 14 734.8 0 14 55 15 734.8 0 15 56 14 734.8 0 14 57 10 734.8 0 10 58 10 734.8 0 10 59 6 734.8 0 6 60 11 734.8 0 11 61 9 734.8 0 9 62 13 734.8 0 13 63 8 734.8 0 8 64 11 734.8 0 11 65 16 734.8 0 16 66 11 734.8 0 11 67 12 734.8 0 12 68 20 734.8 0 20 69 119 734.8 0 119 70 171 734.8 0 171 71 114 734.8 0 114 72 14 734.8 0 14 73 11 734.8 0 11 74 13 734.8 0 13 75 7 734.8 0 7 76 15 734.8 0 15 77 14 734.8 0 14 78 5 734.8 0 5 79 14 734.8 0 14 80 23 734.8 0 23 81 48 734.8 0 48 82 53 734.8 0 53 83 27 734.8 0 27 84 34 734.8 0 34 85 45 734.8 0 45 86 35 734.8 0 35 87 39 734.8 0 39 88 31 734.8 0 31 89 62 734.8 0 62 90 20 734.8 0 20 91 39 734.8 0 39 92 11 734.8 0 11 93 37 734.8 0 37 94 32 734.8 0 32 95 36 734.8 0 36 96 39 734.8 0 39 97 51 734.8 0 51 98 29 734.8 0 29 99 36 734.8 0 36 100 263 734.8 0 263 101 329 734.8 0 329 102 458 734.8 0 458 103 271 734.8 0 271 104 113 734.8 0 113 105 62 734.8 0 62 106 20 734.8 0 20 107 33 734.8 0 33 108 18 734.8 0 18 109 16 734.8 0 16 110 42 734.8 0 42 111 49 734.8 0 49 112 58 734.8 0 58 113 49 734.8 0 49 114 57 734.8 0 57 115 53 734.8 0 53 116 57 734.8 0 57 117 117 734.8 0 117 118 136 734.8 0 136 119 86 734.8 0 86 120 81 734.8 0 81 121 62 734.8 0 62 122 105 734.8 0 105 123 202 734.8 0 202 124 233 734.8 0 233 125 84 734.8 0 84 126 64 734.8 0 64 127 65 734.8 0 65 128 122 734.8 0 122 129 49 734.8 0 49 130 99 734.8 0 99 131 155 734.8 0 155 132 314 734.8 0 314 133 274 734.8 0 274 134 119 734.8 0 119 135 106 734.8 0 106 136 85 734.8 0 85 137 147 734.8 0 147 138 178 734.8 0 178 139 153 734.8 0 153 140 143 734.8 0 143 141 154 734.8 0 154 142 337 734.8 0 337 143 652 734.8 0 652 144 449 734.8 0 449 145 172 734.8 0 172 146 119 734.8 0 119 147 144 734.8 0 144 148 202 734.8 0 202 149 190 734.8 0 190 150 191 734.8 0 191 151 181 734.8 0 181 152 127 734.8 0 127 153 151 734.8 0 151 154 141 734.8 0 141 155 106 734.8 0 106 156 185 734.8 0 185 157 204 734.8 0 204 158 295 734.8 0 295 159 393 734.8 0 393 160 474 734.8 0 474 161 353 734.8 0 353 162 306 734.8 0 306 163 196 734.8 0 196 164 139 734.8 0 139 165 109 734.8 0 109 166 106 734.8 0 106 167 147 734.8 0 147 168 270 734.8 0 270 169 287 734.8 0 287 170 280 734.8 0 280 171 212 734.8 0 212 172 168 734.8 0 168 173 129 734.8 0 129 174 115 734.8 0 115 175 120 734.8 0 120 176 613 734.8 0 613 177 716 734.8 0 716 178 294 734.8 0 294 179 220 734.8 0 220 180 141 734.8 0 141 181 118 734.8 0 118 182 158 734.8 0 158 183 203 734.8 0 203 184 235 734.8 0 235 185 195 734.8 0 195 186 206 734.8 0 206 187 304 734.8 0 304 188 346 734.8 0 346 189 474 734.8 0 474 190 248 734.8 0 248 191 133 734.8 0 133 192 113 734.8 0 113 193 106 734.8 0 106 194 208 734.8 0 208 195 202 734.8 0 202 196 316 734.8 0 316 197 237 734.8 0 237 198 152 734.8 0 152 199 234 734.8 0 234 200 200 734.8 0 200 201 252 734.8 0 252 202 163 734.8 0 163 203 108 734.8 0 108 204 184 734.8 0 184 205 226 734.8 0 226 206 190 734.8 0 190 207 184 734.8 0 184 208 187 734.8 0 187 209 210 734.8 0 210 210 272 734.8 0 272 211 383 734.8 0 383 212 329 734.8 0 329 213 268 734.8 0 268 214 294 734.8 0 294 215 314 734.8 0 314 216 285 734.8 0 285 217 271 734.8 0 271 218 267 734.8 0 267 219 269 734.8 0 269 220 249 734.8 0 249 221 265 734.8 0 265 222 352 734.8 0 352 223 326 734.8 0 326 224 397 734.8 0 397 225 438 734.8 0 438 226 279 734.8 0 279 227 276 734.8 0 276 228 272 734.8 0 272 229 273 734.8 0 273 230 377 734.8 0 377 231 231 734.8 0 231 232 203 734.8 0 203 233 282 734.8 0 282 234 267 734.8 0 267 235 232 734.8 0 232 236 257 734.8 0 257 237 269 734.8 0 269 238 404 734.8 0 404 239 738 734.8 0 738 240 545 734.8 0 545 241 372 734.8 0 372 242 294 734.8 0 294 243 305 734.8 0 305 244 331 734.8 0 331 245 391 734.8 0 391 246 381 734.8 0 381 247 416 734.8 0 416 248 363 734.8 0 363 249 499 734.8 0 499 250 329 734.8 0 329 251 234 734.8 0 234 252 115 734.8 0 115 253 213 734.8 0 213 254 352 734.8 0 352 255 401 734.8 0 401 256 411 734.8 0 411 257 361 734.8 0 361 258 334 734.8 0 334 259 342 734.8 0 342 260 398 734.8 0 398 261 278 734.8 0 278 262 319 734.8 0 319 263 440 734.8 0 440 264 679 734.8 0 679 265 559 734.8 0 559 266 386 734.8 0 386 267 306 734.8 0 306 268 262 734.8 0 262 269 382 734.8 0 382 270 284 734.8 0 284 271 280 734.8 0 280 272 231 734.8 0 231 273 309 734.8 0 309 274 469 734.8 0 469 275 481 734.8 0 481 276 531 734.8 0 531 277 438 734.8 0 438 278 381 734.8 0 381 279 245 734.8 0 245 280 420 734.8 0 420 281 417 734.8 0 417 282 554 734.8 0 554 283 410 734.8 0 410 284 311 734.8 0 311 285 432 734.8 0 432 286 390 734.8 0 390 287 435 734.8 0 435 288 423 734.8 0 423 289 384 734.8 0 384 290 241 734.8 0 241 291 178 734.8 0 178 292 214 734.8 0 214 293 355 734.8 0 355 294 355 734.8 0 355 295 379 734.8 0 379 296 251 734.8 0 251 297 186 734.8 0 186 298 274 734.8 0 274 299 350 734.8 0 350 300 353 734.8 0 353 301 244 734.8 0 244 Finished in 0.96 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 25,410 Reads with adapters: 1,651 (6.5%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 1,639 (6.5%) Total basepairs processed: 4,406,488 bp Total written (filtered): 440,680 bp (10.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1651 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 14 0.0 3 0 13 1 20 1468 0.0 4 1450 16 1 0 1 21 5 0.0 4 0 5 22 3 0.0 4 0 3 23 61 0.0 4 59 2 44 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 74 0.0 4 0 0 0 71 3 112 2 0.0 4 0 0 0 1 1 114 1 0.0 4 0 0 0 1 127 7 0.0 4 0 0 0 0 7 137 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 1,639 Reads with adapters: 517 (31.5%) Reads written (passing filters): 1,639 (100.0%) Total basepairs processed: 440,680 bp Total written (filtered): 308,260 bp (70.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 517 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 94.0% C: 3.9% G: 1.7% T: 0.2% none/other: 0.2% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 2 0.0 1 0 2 11 7 0.0 1 0 7 12 1 0.0 1 0 1 234 1 0.0 1 1 242 1 0.0 1 1 256 1 0.0 1 1 260 1 0.0 1 1 261 483 0.0 1 456 27 262 17 0.0 1 16 1 263 1 0.0 1 1 265 1 0.0 1 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.