This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.35 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 235,578 (7.8%) Reads that were too short: 120,628 (4.0%) Reads written (passing filters): 114,950 (3.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 22,945,001 bp (2.5%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 235578 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 484 734.8 0 484 7 25174 734.8 0 25174 8 67 734.8 0 67 9 2270 734.8 0 2270 10 37 734.8 0 37 11 15 734.8 0 15 12 15 734.8 0 15 13 49 734.8 0 49 14 16 734.8 0 16 15 9 734.8 0 9 16 9 734.8 0 9 17 14 734.8 0 14 18 16 734.8 0 16 19 6 734.8 0 6 20 10 734.8 0 10 21 7 734.8 0 7 22 6 734.8 0 6 23 8 734.8 0 8 24 9 734.8 0 9 25 19 734.8 0 19 26 11 734.8 0 11 27 11 734.8 0 11 28 9 734.8 0 9 29 9 734.8 0 9 30 6 734.8 0 6 31 21 734.8 0 21 32 14 734.8 0 14 33 13 734.8 0 13 34 14 734.8 0 14 35 17 734.8 0 17 36 6 734.8 0 6 37 12 734.8 0 12 38 15 734.8 0 15 39 14 734.8 0 14 40 8 734.8 0 8 41 13 734.8 0 13 42 14 734.8 0 14 43 10 734.8 0 10 44 12 734.8 0 12 45 7 734.8 0 7 46 9 734.8 0 9 47 11 734.8 0 11 48 13 734.8 0 13 49 10 734.8 0 10 50 12 734.8 0 12 51 10 734.8 0 10 52 9 734.8 0 9 53 11 734.8 0 11 54 63 734.8 0 63 55 8648 734.8 0 8648 56 107 734.8 0 107 57 14 734.8 0 14 58 11 734.8 0 11 59 7 734.8 0 7 60 11 734.8 0 11 61 32 734.8 0 32 62 22 734.8 0 22 63 13 734.8 0 13 64 42 734.8 0 42 65 434 734.8 0 434 66 296 734.8 0 296 67 274 734.8 0 274 68 9 734.8 0 9 69 15 734.8 0 15 70 30 734.8 0 30 71 32 734.8 0 32 72 36 734.8 0 36 73 55 734.8 0 55 74 89 734.8 0 89 75 106 734.8 0 106 76 448 734.8 0 448 77 238 734.8 0 238 78 194 734.8 0 194 79 175 734.8 0 175 80 331 734.8 0 331 81 664 734.8 0 664 82 602 734.8 0 602 83 353 734.8 0 353 84 353 734.8 0 353 85 353 734.8 0 353 86 663 734.8 0 663 87 449 734.8 0 449 88 361 734.8 0 361 89 658 734.8 0 658 90 329 734.8 0 329 91 274 734.8 0 274 92 321 734.8 0 321 93 427 734.8 0 427 94 352 734.8 0 352 95 515 734.8 0 515 96 254 734.8 0 254 97 498 734.8 0 498 98 410 734.8 0 410 99 287 734.8 0 287 100 371 734.8 0 371 101 446 734.8 0 446 102 957 734.8 0 957 103 596 734.8 0 596 104 470 734.8 0 470 105 445 734.8 0 445 106 560 734.8 0 560 107 678 734.8 0 678 108 582 734.8 0 582 109 707 734.8 0 707 110 823 734.8 0 823 111 695 734.8 0 695 112 535 734.8 0 535 113 706 734.8 0 706 114 481 734.8 0 481 115 451 734.8 0 451 116 330 734.8 0 330 117 422 734.8 0 422 118 812 734.8 0 812 119 873 734.8 0 873 120 529 734.8 0 529 121 349 734.8 0 349 122 501 734.8 0 501 123 690 734.8 0 690 124 657 734.8 0 657 125 864 734.8 0 864 126 870 734.8 0 870 127 809 734.8 0 809 128 532 734.8 0 532 129 416 734.8 0 416 130 441 734.8 0 441 131 522 734.8 0 522 132 699 734.8 0 699 133 1223 734.8 0 1223 134 990 734.8 0 990 135 539 734.8 0 539 136 509 734.8 0 509 137 585 734.8 0 585 138 902 734.8 0 902 139 1176 734.8 0 1176 140 872 734.8 0 872 141 487 734.8 0 487 142 398 734.8 0 398 143 566 734.8 0 566 144 1009 734.8 0 1009 145 883 734.8 0 883 146 916 734.8 0 916 147 757 734.8 0 757 148 529 734.8 0 529 149 726 734.8 0 726 150 625 734.8 0 625 151 912 734.8 0 912 152 974 734.8 0 974 153 736 734.8 0 736 154 821 734.8 0 821 155 562 734.8 0 562 156 755 734.8 0 755 157 1104 734.8 0 1104 158 634 734.8 0 634 159 848 734.8 0 848 160 662 734.8 0 662 161 623 734.8 0 623 162 532 734.8 0 532 163 510 734.8 0 510 164 879 734.8 0 879 165 768 734.8 0 768 166 588 734.8 0 588 167 462 734.8 0 462 168 493 734.8 0 493 169 467 734.8 0 467 170 466 734.8 0 466 171 520 734.8 0 520 172 833 734.8 0 833 173 674 734.8 0 674 174 537 734.8 0 537 175 332 734.8 0 332 176 479 734.8 0 479 177 487 734.8 0 487 178 453 734.8 0 453 179 740 734.8 0 740 180 844 734.8 0 844 181 881 734.8 0 881 182 542 734.8 0 542 183 476 734.8 0 476 184 686 734.8 0 686 185 754 734.8 0 754 186 628 734.8 0 628 187 489 734.8 0 489 188 612 734.8 0 612 189 760 734.8 0 760 190 778 734.8 0 778 191 604 734.8 0 604 192 496 734.8 0 496 193 481 734.8 0 481 194 552 734.8 0 552 195 451 734.8 0 451 196 684 734.8 0 684 197 764 734.8 0 764 198 398 734.8 0 398 199 810 734.8 0 810 200 658 734.8 0 658 201 824 734.8 0 824 202 863 734.8 0 863 203 530 734.8 0 530 204 534 734.8 0 534 205 723 734.8 0 723 206 988 734.8 0 988 207 861 734.8 0 861 208 788 734.8 0 788 209 881 734.8 0 881 210 1160 734.8 0 1160 211 5078 734.8 0 5078 212 19899 734.8 0 19899 213 4049 734.8 0 4049 214 1513 734.8 0 1513 215 693 734.8 0 693 216 551 734.8 0 551 217 480 734.8 0 480 218 700 734.8 0 700 219 872 734.8 0 872 220 916 734.8 0 916 221 551 734.8 0 551 222 664 734.8 0 664 223 692 734.8 0 692 224 817 734.8 0 817 225 791 734.8 0 791 226 692 734.8 0 692 227 525 734.8 0 525 228 525 734.8 0 525 229 629 734.8 0 629 230 640 734.8 0 640 231 612 734.8 0 612 232 607 734.8 0 607 233 665 734.8 0 665 234 760 734.8 0 760 235 597 734.8 0 597 236 695 734.8 0 695 237 549 734.8 0 549 238 1040 734.8 0 1040 239 872 734.8 0 872 240 899 734.8 0 899 241 978 734.8 0 978 242 819 734.8 0 819 243 661 734.8 0 661 244 531 734.8 0 531 245 772 734.8 0 772 246 620 734.8 0 620 247 579 734.8 0 579 248 441 734.8 0 441 249 743 734.8 0 743 250 801 734.8 0 801 251 1030 734.8 0 1030 252 976 734.8 0 976 253 529 734.8 0 529 254 561 734.8 0 561 255 642 734.8 0 642 256 657 734.8 0 657 257 640 734.8 0 640 258 604 734.8 0 604 259 760 734.8 0 760 260 923 734.8 0 923 261 1048 734.8 0 1048 262 974 734.8 0 974 263 700 734.8 0 700 264 581 734.8 0 581 265 704 734.8 0 704 266 716 734.8 0 716 267 835 734.8 0 835 268 660 734.8 0 660 269 663 734.8 0 663 270 1469 734.8 0 1469 271 2800 734.8 0 2800 272 1199 734.8 0 1199 273 836 734.8 0 836 274 1871 734.8 0 1871 275 1044 734.8 0 1044 276 1072 734.8 0 1072 277 1312 734.8 0 1312 278 1324 734.8 0 1324 279 812 734.8 0 812 280 660 734.8 0 660 281 573 734.8 0 573 282 846 734.8 0 846 283 1039 734.8 0 1039 284 749 734.8 0 749 285 584 734.8 0 584 286 801 734.8 0 801 287 737 734.8 0 737 288 1017 734.8 0 1017 289 1148 734.8 0 1148 290 708 734.8 0 708 291 930 734.8 0 930 292 1980 734.8 0 1980 293 4630 734.8 0 4630 294 4013 734.8 0 4013 295 1433 734.8 0 1433 296 888 734.8 0 888 297 836 734.8 0 836 298 904 734.8 0 904 299 991 734.8 0 991 300 1517 734.8 0 1517 301 2856 734.8 0 2856 Finished in 4.04 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 114,950 Reads with adapters: 2,340 (2.0%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 2,295 (2.0%) Total basepairs processed: 22,945,001 bp Total written (filtered): 624,905 bp (2.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2340 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 37 0.0 3 0 33 2 2 17 2192 0.0 3 2127 58 4 3 18 15 0.0 3 1 11 1 2 19 6 0.0 3 0 0 2 4 20 2 0.0 3 0 0 1 1 21 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 1 26 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 7 0.0 3 0 0 0 7 283 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 2,295 Reads with adapters: 79 (3.4%) Reads written (passing filters): 2,295 (100.0%) Total basepairs processed: 624,905 bp Total written (filtered): 624,666 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 79 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.8% C: 5.1% G: 25.3% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 77 35.9 0 77 4 2 9.0 0 2