This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.52 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 64,461 (2.1%) Reads that were too short: 36,400 (1.2%) Reads written (passing filters): 28,061 (0.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 7,265,787 bp (0.8%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 64461 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1501 734.8 0 1501 7 176 734.8 0 176 8 15186 734.8 0 15186 9 2605 734.8 0 2605 10 27 734.8 0 27 11 17 734.8 0 17 12 75 734.8 0 75 13 74 734.8 0 74 14 10 734.8 0 10 15 22 734.8 0 22 16 26 734.8 0 26 17 21 734.8 0 21 18 20 734.8 0 20 19 29 734.8 0 29 20 37 734.8 0 37 21 23 734.8 0 23 22 26 734.8 0 26 23 14 734.8 0 14 24 37 734.8 0 37 25 21 734.8 0 21 26 18 734.8 0 18 27 29 734.8 0 29 28 35 734.8 0 35 29 27 734.8 0 27 30 30 734.8 0 30 31 30 734.8 0 30 32 30 734.8 0 30 33 19 734.8 0 19 34 38 734.8 0 38 35 26 734.8 0 26 36 23 734.8 0 23 37 33 734.8 0 33 38 35 734.8 0 35 39 28 734.8 0 28 40 304 734.8 0 304 41 22 734.8 0 22 42 23 734.8 0 23 43 33 734.8 0 33 44 39 734.8 0 39 45 23 734.8 0 23 46 23 734.8 0 23 47 23 734.8 0 23 48 19 734.8 0 19 49 26 734.8 0 26 50 21 734.8 0 21 51 47 734.8 0 47 52 58 734.8 0 58 53 26 734.8 0 26 54 29 734.8 0 29 55 20 734.8 0 20 56 18 734.8 0 18 57 22 734.8 0 22 58 26 734.8 0 26 59 26 734.8 0 26 60 26 734.8 0 26 61 31 734.8 0 31 62 36 734.8 0 36 63 27 734.8 0 27 64 26 734.8 0 26 65 33 734.8 0 33 66 35 734.8 0 35 67 37 734.8 0 37 68 41 734.8 0 41 69 38 734.8 0 38 70 23 734.8 0 23 71 29 734.8 0 29 72 20 734.8 0 20 73 12 734.8 0 12 74 31 734.8 0 31 75 43 734.8 0 43 76 35 734.8 0 35 77 21 734.8 0 21 78 20 734.8 0 20 79 31 734.8 0 31 80 33 734.8 0 33 81 21 734.8 0 21 82 28 734.8 0 28 83 33 734.8 0 33 84 21 734.8 0 21 85 30 734.8 0 30 86 19 734.8 0 19 87 24 734.8 0 24 88 22 734.8 0 22 89 26 734.8 0 26 90 28 734.8 0 28 91 24 734.8 0 24 92 33 734.8 0 33 93 36 734.8 0 36 94 25 734.8 0 25 95 30 734.8 0 30 96 40 734.8 0 40 97 56 734.8 0 56 98 40 734.8 0 40 99 42 734.8 0 42 100 40 734.8 0 40 101 71 734.8 0 71 102 86 734.8 0 86 103 49 734.8 0 49 104 33 734.8 0 33 105 29 734.8 0 29 106 35 734.8 0 35 107 32 734.8 0 32 108 25 734.8 0 25 109 29 734.8 0 29 110 21 734.8 0 21 111 27 734.8 0 27 112 22 734.8 0 22 113 37 734.8 0 37 114 24 734.8 0 24 115 23 734.8 0 23 116 64 734.8 0 64 117 72 734.8 0 72 118 64 734.8 0 64 119 52 734.8 0 52 120 33 734.8 0 33 121 41 734.8 0 41 122 32 734.8 0 32 123 41 734.8 0 41 124 33 734.8 0 33 125 46 734.8 0 46 126 37 734.8 0 37 127 41 734.8 0 41 128 27 734.8 0 27 129 26 734.8 0 26 130 38 734.8 0 38 131 38 734.8 0 38 132 55 734.8 0 55 133 61 734.8 0 61 134 39 734.8 0 39 135 46 734.8 0 46 136 52 734.8 0 52 137 90 734.8 0 90 138 370 734.8 0 370 139 297 734.8 0 297 140 120 734.8 0 120 141 44 734.8 0 44 142 47 734.8 0 47 143 55 734.8 0 55 144 57 734.8 0 57 145 67 734.8 0 67 146 53 734.8 0 53 147 31 734.8 0 31 148 46 734.8 0 46 149 44 734.8 0 44 150 38 734.8 0 38 151 56 734.8 0 56 152 45 734.8 0 45 153 30 734.8 0 30 154 27 734.8 0 27 155 34 734.8 0 34 156 41 734.8 0 41 157 47 734.8 0 47 158 58 734.8 0 58 159 55 734.8 0 55 160 59 734.8 0 59 161 48 734.8 0 48 162 36 734.8 0 36 163 41 734.8 0 41 164 31 734.8 0 31 165 41 734.8 0 41 166 53 734.8 0 53 167 59 734.8 0 59 168 35 734.8 0 35 169 55 734.8 0 55 170 59 734.8 0 59 171 67 734.8 0 67 172 57 734.8 0 57 173 41 734.8 0 41 174 48 734.8 0 48 175 50 734.8 0 50 176 51 734.8 0 51 177 50 734.8 0 50 178 59 734.8 0 59 179 67 734.8 0 67 180 86 734.8 0 86 181 47 734.8 0 47 182 48 734.8 0 48 183 45 734.8 0 45 184 48 734.8 0 48 185 63 734.8 0 63 186 57 734.8 0 57 187 116 734.8 0 116 188 156 734.8 0 156 189 59 734.8 0 59 190 35 734.8 0 35 191 39 734.8 0 39 192 53 734.8 0 53 193 47 734.8 0 47 194 82 734.8 0 82 195 70 734.8 0 70 196 49 734.8 0 49 197 47 734.8 0 47 198 53 734.8 0 53 199 75 734.8 0 75 200 80 734.8 0 80 201 44 734.8 0 44 202 40 734.8 0 40 203 31 734.8 0 31 204 52 734.8 0 52 205 47 734.8 0 47 206 60 734.8 0 60 207 47 734.8 0 47 208 59 734.8 0 59 209 56 734.8 0 56 210 50 734.8 0 50 211 49 734.8 0 49 212 62 734.8 0 62 213 51 734.8 0 51 214 97 734.8 0 97 215 88 734.8 0 88 216 72 734.8 0 72 217 57 734.8 0 57 218 81 734.8 0 81 219 69 734.8 0 69 220 62 734.8 0 62 221 46 734.8 0 46 222 44 734.8 0 44 223 37 734.8 0 37 224 52 734.8 0 52 225 47 734.8 0 47 226 56 734.8 0 56 227 103 734.8 0 103 228 77 734.8 0 77 229 60 734.8 0 60 230 48 734.8 0 48 231 57 734.8 0 57 232 47 734.8 0 47 233 61 734.8 0 61 234 46 734.8 0 46 235 40 734.8 0 40 236 49 734.8 0 49 237 59 734.8 0 59 238 70 734.8 0 70 239 78 734.8 0 78 240 61 734.8 0 61 241 48 734.8 0 48 242 39 734.8 0 39 243 41 734.8 0 41 244 49 734.8 0 49 245 39 734.8 0 39 246 37 734.8 0 37 247 49 734.8 0 49 248 79 734.8 0 79 249 55 734.8 0 55 250 37 734.8 0 37 251 39 734.8 0 39 252 46 734.8 0 46 253 39 734.8 0 39 254 49 734.8 0 49 255 54 734.8 0 54 256 61 734.8 0 61 257 56 734.8 0 56 258 81 734.8 0 81 259 75 734.8 0 75 260 97 734.8 0 97 261 43 734.8 0 43 262 74 734.8 0 74 263 61 734.8 0 61 264 73 734.8 0 73 265 50 734.8 0 50 266 81 734.8 0 81 267 66 734.8 0 66 268 95 734.8 0 95 269 109 734.8 0 109 270 80 734.8 0 80 271 80 734.8 0 80 272 109 734.8 0 109 273 1347 734.8 0 1347 274 5739 734.8 0 5739 275 561 734.8 0 561 276 83 734.8 0 83 277 88 734.8 0 88 278 62 734.8 0 62 279 73 734.8 0 73 280 142 734.8 0 142 281 116 734.8 0 116 282 80 734.8 0 80 283 117 734.8 0 117 284 194 734.8 0 194 285 244 734.8 0 244 286 340 734.8 0 340 287 219 734.8 0 219 288 217 734.8 0 217 289 149 734.8 0 149 290 132 734.8 0 132 291 98 734.8 0 98 292 212 734.8 0 212 293 2352 734.8 0 2352 294 3391 734.8 0 3391 295 1704 734.8 0 1704 296 732 734.8 0 732 297 1244 734.8 0 1244 298 1857 734.8 0 1857 299 4834 734.8 0 4834 300 4725 734.8 0 4725 301 1089 734.8 0 1089 Finished in 1.44 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 28,061 Reads with adapters: 4,438 (15.8%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 4,435 (15.8%) Total basepairs processed: 7,265,787 bp Total written (filtered): 1,139,785 bp (15.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4438 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 2 0.0 3 0 0 2 19 43 0.0 3 0 40 0 2 1 20 2604 0.0 4 2565 36 1 0 2 21 23 0.0 4 2 19 1 0 1 22 1103 0.0 4 1094 9 23 7 0.0 4 3 2 1 1 24 2 0.0 4 0 0 2 42 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 1 1 105 6 0.0 4 0 0 0 6 107 51 0.0 4 0 0 0 45 6 117 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 4 0.0 4 0 0 0 0 4 127 577 0.0 4 0 0 0 0 577 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 4,435 Reads with adapters: 132 (3.0%) Reads written (passing filters): 4,435 (100.0%) Total basepairs processed: 1,139,785 bp Total written (filtered): 1,136,899 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 132 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.3% C: 0.8% G: 91.7% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 1.1 0 1 9 8 0.0 0 0 8 10 46 0.0 1 1 45 11 70 0.0 1 0 70 13 1 0.0 1 0 1 259 5 0.0 1 5 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.