This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.89 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 213,082 (7.1%) Reads that were too short: 130,128 (4.3%) Reads written (passing filters): 82,954 (2.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 19,225,359 bp (2.1%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 213082 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 719 734.8 0 719 7 537 734.8 0 537 8 39438 734.8 0 39438 9 4679 734.8 0 4679 10 44 734.8 0 44 11 56 734.8 0 56 12 35 734.8 0 35 13 53 734.8 0 53 14 47 734.8 0 47 15 26 734.8 0 26 16 40 734.8 0 40 17 33 734.8 0 33 18 90 734.8 0 90 19 34 734.8 0 34 20 37 734.8 0 37 21 33 734.8 0 33 22 31 734.8 0 31 23 29 734.8 0 29 24 39 734.8 0 39 25 26 734.8 0 26 26 30 734.8 0 30 27 35 734.8 0 35 28 37 734.8 0 37 29 17 734.8 0 17 30 28 734.8 0 28 31 36 734.8 0 36 32 37 734.8 0 37 33 25 734.8 0 25 34 29 734.8 0 29 35 29 734.8 0 29 36 38 734.8 0 38 37 91 734.8 0 91 38 106 734.8 0 106 39 56 734.8 0 56 40 29 734.8 0 29 41 156 734.8 0 156 42 52 734.8 0 52 43 39 734.8 0 39 44 131 734.8 0 131 45 42 734.8 0 42 46 76 734.8 0 76 47 99 734.8 0 99 48 103 734.8 0 103 49 48 734.8 0 48 50 37 734.8 0 37 51 44 734.8 0 44 52 82 734.8 0 82 53 215 734.8 0 215 54 233 734.8 0 233 55 75 734.8 0 75 56 46 734.8 0 46 57 444 734.8 0 444 58 57 734.8 0 57 59 28 734.8 0 28 60 27 734.8 0 27 61 36 734.8 0 36 62 31 734.8 0 31 63 24 734.8 0 24 64 36 734.8 0 36 65 47 734.8 0 47 66 59 734.8 0 59 67 35 734.8 0 35 68 40 734.8 0 40 69 31 734.8 0 31 70 28 734.8 0 28 71 27 734.8 0 27 72 57 734.8 0 57 73 291 734.8 0 291 74 152 734.8 0 152 75 149 734.8 0 149 76 72 734.8 0 72 77 82 734.8 0 82 78 89 734.8 0 89 79 76 734.8 0 76 80 43 734.8 0 43 81 128 734.8 0 128 82 108 734.8 0 108 83 98 734.8 0 98 84 107 734.8 0 107 85 156 734.8 0 156 86 144 734.8 0 144 87 100 734.8 0 100 88 115 734.8 0 115 89 108 734.8 0 108 90 83 734.8 0 83 91 145 734.8 0 145 92 103 734.8 0 103 93 168 734.8 0 168 94 245 734.8 0 245 95 149 734.8 0 149 96 148 734.8 0 148 97 177 734.8 0 177 98 236 734.8 0 236 99 135 734.8 0 135 100 55 734.8 0 55 101 45 734.8 0 45 102 48 734.8 0 48 103 76 734.8 0 76 104 43 734.8 0 43 105 35 734.8 0 35 106 75 734.8 0 75 107 115 734.8 0 115 108 94 734.8 0 94 109 82 734.8 0 82 110 95 734.8 0 95 111 83 734.8 0 83 112 86 734.8 0 86 113 82 734.8 0 82 114 129 734.8 0 129 115 195 734.8 0 195 116 184 734.8 0 184 117 125 734.8 0 125 118 123 734.8 0 123 119 203 734.8 0 203 120 263 734.8 0 263 121 157 734.8 0 157 122 101 734.8 0 101 123 117 734.8 0 117 124 136 734.8 0 136 125 187 734.8 0 187 126 214 734.8 0 214 127 250 734.8 0 250 128 253 734.8 0 253 129 197 734.8 0 197 130 172 734.8 0 172 131 145 734.8 0 145 132 123 734.8 0 123 133 161 734.8 0 161 134 291 734.8 0 291 135 346 734.8 0 346 136 326 734.8 0 326 137 288 734.8 0 288 138 174 734.8 0 174 139 134 734.8 0 134 140 189 734.8 0 189 141 211 734.8 0 211 142 136 734.8 0 136 143 133 734.8 0 133 144 202 734.8 0 202 145 1237 734.8 0 1237 146 672 734.8 0 672 147 472 734.8 0 472 148 224 734.8 0 224 149 159 734.8 0 159 150 170 734.8 0 170 151 187 734.8 0 187 152 242 734.8 0 242 153 281 734.8 0 281 154 352 734.8 0 352 155 317 734.8 0 317 156 298 734.8 0 298 157 351 734.8 0 351 158 282 734.8 0 282 159 251 734.8 0 251 160 251 734.8 0 251 161 263 734.8 0 263 162 241 734.8 0 241 163 195 734.8 0 195 164 256 734.8 0 256 165 530 734.8 0 530 166 485 734.8 0 485 167 296 734.8 0 296 168 199 734.8 0 199 169 203 734.8 0 203 170 211 734.8 0 211 171 214 734.8 0 214 172 237 734.8 0 237 173 1102 734.8 0 1102 174 2303 734.8 0 2303 175 1451 734.8 0 1451 176 448 734.8 0 448 177 185 734.8 0 185 178 211 734.8 0 211 179 281 734.8 0 281 180 312 734.8 0 312 181 577 734.8 0 577 182 733 734.8 0 733 183 529 734.8 0 529 184 296 734.8 0 296 185 354 734.8 0 354 186 627 734.8 0 627 187 512 734.8 0 512 188 347 734.8 0 347 189 268 734.8 0 268 190 250 734.8 0 250 191 350 734.8 0 350 192 496 734.8 0 496 193 702 734.8 0 702 194 342 734.8 0 342 195 191 734.8 0 191 196 164 734.8 0 164 197 228 734.8 0 228 198 309 734.8 0 309 199 279 734.8 0 279 200 165 734.8 0 165 201 246 734.8 0 246 202 310 734.8 0 310 203 315 734.8 0 315 204 347 734.8 0 347 205 327 734.8 0 327 206 509 734.8 0 509 207 462 734.8 0 462 208 295 734.8 0 295 209 219 734.8 0 219 210 218 734.8 0 218 211 238 734.8 0 238 212 299 734.8 0 299 213 316 734.8 0 316 214 348 734.8 0 348 215 347 734.8 0 347 216 327 734.8 0 327 217 351 734.8 0 351 218 408 734.8 0 408 219 549 734.8 0 549 220 463 734.8 0 463 221 474 734.8 0 474 222 453 734.8 0 453 223 454 734.8 0 454 224 418 734.8 0 418 225 4063 734.8 0 4063 226 9173 734.8 0 9173 227 1699 734.8 0 1699 228 318 734.8 0 318 229 442 734.8 0 442 230 2504 734.8 0 2504 231 4235 734.8 0 4235 232 3304 734.8 0 3304 233 2154 734.8 0 2154 234 1031 734.8 0 1031 235 707 734.8 0 707 236 297 734.8 0 297 237 256 734.8 0 256 238 320 734.8 0 320 239 384 734.8 0 384 240 222 734.8 0 222 241 322 734.8 0 322 242 394 734.8 0 394 243 338 734.8 0 338 244 332 734.8 0 332 245 325 734.8 0 325 246 707 734.8 0 707 247 633 734.8 0 633 248 396 734.8 0 396 249 184 734.8 0 184 250 9076 734.8 0 9076 251 10982 734.8 0 10982 252 4825 734.8 0 4825 253 2183 734.8 0 2183 254 1366 734.8 0 1366 255 551 734.8 0 551 256 447 734.8 0 447 257 346 734.8 0 346 258 222 734.8 0 222 259 131 734.8 0 131 260 172 734.8 0 172 261 187 734.8 0 187 262 278 734.8 0 278 263 246 734.8 0 246 264 253 734.8 0 253 265 570 734.8 0 570 266 549 734.8 0 549 267 503 734.8 0 503 268 344 734.8 0 344 269 252 734.8 0 252 270 388 734.8 0 388 271 902 734.8 0 902 272 2259 734.8 0 2259 273 434 734.8 0 434 274 369 734.8 0 369 275 448 734.8 0 448 276 418 734.8 0 418 277 434 734.8 0 434 278 639 734.8 0 639 279 859 734.8 0 859 280 2456 734.8 0 2456 281 2370 734.8 0 2370 282 1378 734.8 0 1378 283 457 734.8 0 457 284 559 734.8 0 559 285 1225 734.8 0 1225 286 749 734.8 0 749 287 417 734.8 0 417 288 409 734.8 0 409 289 455 734.8 0 455 290 880 734.8 0 880 291 883 734.8 0 883 292 763 734.8 0 763 293 473 734.8 0 473 294 702 734.8 0 702 295 809 734.8 0 809 296 1010 734.8 0 1010 297 1233 734.8 0 1233 298 1994 734.8 0 1994 299 3150 734.8 0 3150 300 4359 734.8 0 4359 301 19577 734.8 0 19577 Finished in 3.41 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 82,954 Reads with adapters: 40,468 (48.8%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 40,429 (48.7%) Total basepairs processed: 19,225,359 bp Total written (filtered): 11,152,398 bp (58.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 40468 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 27 0.0 3 0 0 20 7 16 366 0.0 3 0 330 24 12 17 39746 0.0 3 38657 942 114 33 18 100 0.0 3 4 82 7 7 19 66 0.0 3 0 0 41 25 20 72 0.0 3 12 0 0 60 21 2 0.0 3 1 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 21 0.0 3 0 0 0 21 49 9 0.0 3 0 0 0 9 56 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 94 5 0.0 3 0 0 0 5 97 5 0.0 3 0 0 0 5 99 2 0.0 3 0 0 0 2 100 6 0.0 3 0 0 1 5 101 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 5 0.0 3 0 0 0 5 120 1 0.0 3 0 0 1 126 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.44 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 40,429 Reads with adapters: 1,015 (2.5%) Reads written (passing filters): 40,429 (100.0%) Total basepairs processed: 11,152,398 bp Total written (filtered): 11,149,305 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1015 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.4% C: 8.3% G: 20.5% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 969 631.7 0 969 4 44 157.9 0 44 5 2 39.5 0 2