This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 80.52 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 102,870 (3.4%) Reads that were too short: 35,319 (1.2%) Reads written (passing filters): 67,551 (2.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 18,046,665 bp (2.0%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 102870 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 823 734.8 0 823 7 48833 734.8 0 48833 8 2953 734.8 0 2953 9 28 734.8 0 28 10 11 734.8 0 11 11 33 734.8 0 33 12 21 734.8 0 21 13 25 734.8 0 25 14 23 734.8 0 23 15 28 734.8 0 28 16 26 734.8 0 26 17 34 734.8 0 34 18 19 734.8 0 19 19 37 734.8 0 37 20 41 734.8 0 41 21 46 734.8 0 46 22 43 734.8 0 43 23 90 734.8 0 90 24 30 734.8 0 30 25 27 734.8 0 27 26 33 734.8 0 33 27 22 734.8 0 22 28 15 734.8 0 15 29 30 734.8 0 30 30 29 734.8 0 29 31 47 734.8 0 47 32 34 734.8 0 34 33 29 734.8 0 29 34 32 734.8 0 32 35 32 734.8 0 32 36 22 734.8 0 22 37 9 734.8 0 9 38 31 734.8 0 31 39 197 734.8 0 197 40 67 734.8 0 67 41 50 734.8 0 50 42 29 734.8 0 29 43 47 734.8 0 47 44 26 734.8 0 26 45 23 734.8 0 23 46 29 734.8 0 29 47 24 734.8 0 24 48 20 734.8 0 20 49 21 734.8 0 21 50 34 734.8 0 34 51 23 734.8 0 23 52 36 734.8 0 36 53 40 734.8 0 40 54 30 734.8 0 30 55 27 734.8 0 27 56 27 734.8 0 27 57 24 734.8 0 24 58 39 734.8 0 39 59 21 734.8 0 21 60 47 734.8 0 47 61 42 734.8 0 42 62 27 734.8 0 27 63 13 734.8 0 13 64 22 734.8 0 22 65 26 734.8 0 26 66 30 734.8 0 30 67 26 734.8 0 26 68 23 734.8 0 23 69 20 734.8 0 20 70 45 734.8 0 45 71 60 734.8 0 60 72 36 734.8 0 36 73 32 734.8 0 32 74 28 734.8 0 28 75 28 734.8 0 28 76 40 734.8 0 40 77 28 734.8 0 28 78 44 734.8 0 44 79 39 734.8 0 39 80 60 734.8 0 60 81 55 734.8 0 55 82 62 734.8 0 62 83 69 734.8 0 69 84 51 734.8 0 51 85 123 734.8 0 123 86 182 734.8 0 182 87 63 734.8 0 63 88 39 734.8 0 39 89 51 734.8 0 51 90 47 734.8 0 47 91 67 734.8 0 67 92 143 734.8 0 143 93 89 734.8 0 89 94 79 734.8 0 79 95 109 734.8 0 109 96 88 734.8 0 88 97 72 734.8 0 72 98 286 734.8 0 286 99 275 734.8 0 275 100 124 734.8 0 124 101 95 734.8 0 95 102 105 734.8 0 105 103 143 734.8 0 143 104 89 734.8 0 89 105 45 734.8 0 45 106 35 734.8 0 35 107 37 734.8 0 37 108 28 734.8 0 28 109 45 734.8 0 45 110 53 734.8 0 53 111 132 734.8 0 132 112 102 734.8 0 102 113 103 734.8 0 103 114 71 734.8 0 71 115 54 734.8 0 54 116 96 734.8 0 96 117 98 734.8 0 98 118 93 734.8 0 93 119 62 734.8 0 62 120 64 734.8 0 64 121 59 734.8 0 59 122 51 734.8 0 51 123 90 734.8 0 90 124 167 734.8 0 167 125 133 734.8 0 133 126 57 734.8 0 57 127 66 734.8 0 66 128 61 734.8 0 61 129 52 734.8 0 52 130 68 734.8 0 68 131 106 734.8 0 106 132 117 734.8 0 117 133 144 734.8 0 144 134 81 734.8 0 81 135 84 734.8 0 84 136 66 734.8 0 66 137 95 734.8 0 95 138 149 734.8 0 149 139 135 734.8 0 135 140 91 734.8 0 91 141 75 734.8 0 75 142 128 734.8 0 128 143 195 734.8 0 195 144 196 734.8 0 196 145 109 734.8 0 109 146 72 734.8 0 72 147 109 734.8 0 109 148 96 734.8 0 96 149 86 734.8 0 86 150 91 734.8 0 91 151 101 734.8 0 101 152 89 734.8 0 89 153 90 734.8 0 90 154 66 734.8 0 66 155 61 734.8 0 61 156 67 734.8 0 67 157 85 734.8 0 85 158 131 734.8 0 131 159 167 734.8 0 167 160 106 734.8 0 106 161 81 734.8 0 81 162 159 734.8 0 159 163 113 734.8 0 113 164 79 734.8 0 79 165 73 734.8 0 73 166 80 734.8 0 80 167 105 734.8 0 105 168 113 734.8 0 113 169 147 734.8 0 147 170 192 734.8 0 192 171 200 734.8 0 200 172 134 734.8 0 134 173 87 734.8 0 87 174 47 734.8 0 47 175 49 734.8 0 49 176 74 734.8 0 74 177 139 734.8 0 139 178 190 734.8 0 190 179 152 734.8 0 152 180 125 734.8 0 125 181 72 734.8 0 72 182 69 734.8 0 69 183 135 734.8 0 135 184 210 734.8 0 210 185 131 734.8 0 131 186 93 734.8 0 93 187 191 734.8 0 191 188 198 734.8 0 198 189 140 734.8 0 140 190 88 734.8 0 88 191 65 734.8 0 65 192 89 734.8 0 89 193 118 734.8 0 118 194 138 734.8 0 138 195 113 734.8 0 113 196 82 734.8 0 82 197 83 734.8 0 83 198 187 734.8 0 187 199 180 734.8 0 180 200 78 734.8 0 78 201 50 734.8 0 50 202 51 734.8 0 51 203 59 734.8 0 59 204 112 734.8 0 112 205 101 734.8 0 101 206 116 734.8 0 116 207 115 734.8 0 115 208 91 734.8 0 91 209 66 734.8 0 66 210 130 734.8 0 130 211 212 734.8 0 212 212 180 734.8 0 180 213 169 734.8 0 169 214 968 734.8 0 968 215 1120 734.8 0 1120 216 461 734.8 0 461 217 110 734.8 0 110 218 108 734.8 0 108 219 125 734.8 0 125 220 99 734.8 0 99 221 61 734.8 0 61 222 71 734.8 0 71 223 324 734.8 0 324 224 589 734.8 0 589 225 201 734.8 0 201 226 82 734.8 0 82 227 119 734.8 0 119 228 143 734.8 0 143 229 184 734.8 0 184 230 81 734.8 0 81 231 61 734.8 0 61 232 47 734.8 0 47 233 46 734.8 0 46 234 79 734.8 0 79 235 136 734.8 0 136 236 121 734.8 0 121 237 115 734.8 0 115 238 177 734.8 0 177 239 201 734.8 0 201 240 176 734.8 0 176 241 87 734.8 0 87 242 65 734.8 0 65 243 76 734.8 0 76 244 72 734.8 0 72 245 107 734.8 0 107 246 215 734.8 0 215 247 233 734.8 0 233 248 115 734.8 0 115 249 58 734.8 0 58 250 37 734.8 0 37 251 34 734.8 0 34 252 69 734.8 0 69 253 84 734.8 0 84 254 90 734.8 0 90 255 165 734.8 0 165 256 206 734.8 0 206 257 309 734.8 0 309 258 224 734.8 0 224 259 199 734.8 0 199 260 239 734.8 0 239 261 53 734.8 0 53 262 58 734.8 0 58 263 132 734.8 0 132 264 188 734.8 0 188 265 174 734.8 0 174 266 129 734.8 0 129 267 259 734.8 0 259 268 303 734.8 0 303 269 316 734.8 0 316 270 220 734.8 0 220 271 425 734.8 0 425 272 5125 734.8 0 5125 273 7629 734.8 0 7629 274 4514 734.8 0 4514 275 139 734.8 0 139 276 71 734.8 0 71 277 137 734.8 0 137 278 136 734.8 0 136 279 293 734.8 0 293 280 125 734.8 0 125 281 61 734.8 0 61 282 63 734.8 0 63 283 167 734.8 0 167 284 283 734.8 0 283 285 472 734.8 0 472 286 266 734.8 0 266 287 158 734.8 0 158 288 83 734.8 0 83 289 49 734.8 0 49 290 49 734.8 0 49 291 62 734.8 0 62 292 85 734.8 0 85 293 117 734.8 0 117 294 131 734.8 0 131 295 96 734.8 0 96 296 80 734.8 0 80 297 117 734.8 0 117 298 195 734.8 0 195 299 713 734.8 0 713 300 751 734.8 0 751 301 804 734.8 0 804 Finished in 2.95 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 67,551 Reads with adapters: 49,333 (73.0%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 49,317 (73.0%) Total basepairs processed: 18,046,665 bp Total written (filtered): 13,658,159 bp (75.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49333 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 12 0.0 3 0 0 9 3 16 320 0.0 3 0 279 25 16 17 48718 0.0 3 47196 1308 171 43 18 151 0.0 3 11 129 8 3 19 31 0.0 3 0 0 19 12 20 62 0.0 3 0 0 0 62 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 2 0.0 3 1 0 0 1 32 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 132 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 1 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.74 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 49,317 Reads with adapters: 2,250 (4.6%) Reads written (passing filters): 49,317 (100.0%) Total basepairs processed: 13,658,159 bp Total written (filtered): 13,651,361 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2250 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.7% C: 49.8% G: 15.3% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2205 770.6 0 2205 4 42 192.6 0 42 5 3 48.2 0 3