This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.86 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 166,519 (5.5%) Reads that were too short: 70,753 (2.4%) Reads written (passing filters): 95,766 (3.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 22,776,703 bp (2.5%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 166519 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 489 734.8 0 489 7 50511 734.8 0 50511 8 104 734.8 0 104 9 3822 734.8 0 3822 10 32 734.8 0 32 11 15 734.8 0 15 12 11 734.8 0 11 13 17 734.8 0 17 14 17 734.8 0 17 15 46 734.8 0 46 16 43 734.8 0 43 17 19 734.8 0 19 18 24 734.8 0 24 19 24 734.8 0 24 20 11 734.8 0 11 21 9 734.8 0 9 22 18 734.8 0 18 23 19 734.8 0 19 24 15 734.8 0 15 25 20 734.8 0 20 26 29 734.8 0 29 27 830 734.8 0 830 28 98 734.8 0 98 29 24 734.8 0 24 30 15 734.8 0 15 31 18 734.8 0 18 32 18 734.8 0 18 33 21 734.8 0 21 34 19 734.8 0 19 35 14 734.8 0 14 36 16 734.8 0 16 37 14 734.8 0 14 38 7 734.8 0 7 39 301 734.8 0 301 40 27 734.8 0 27 41 20 734.8 0 20 42 20 734.8 0 20 43 24 734.8 0 24 44 19 734.8 0 19 45 18 734.8 0 18 46 18 734.8 0 18 47 17 734.8 0 17 48 27 734.8 0 27 49 11 734.8 0 11 50 19 734.8 0 19 51 17 734.8 0 17 52 16 734.8 0 16 53 13 734.8 0 13 54 27 734.8 0 27 55 22 734.8 0 22 56 24 734.8 0 24 57 782 734.8 0 782 58 304 734.8 0 304 59 35 734.8 0 35 60 16 734.8 0 16 61 16 734.8 0 16 62 23 734.8 0 23 63 29 734.8 0 29 64 141 734.8 0 141 65 18 734.8 0 18 66 11 734.8 0 11 67 19 734.8 0 19 68 28 734.8 0 28 69 20 734.8 0 20 70 18 734.8 0 18 71 21 734.8 0 21 72 21 734.8 0 21 73 22 734.8 0 22 74 18 734.8 0 18 75 16 734.8 0 16 76 16 734.8 0 16 77 23 734.8 0 23 78 31 734.8 0 31 79 37 734.8 0 37 80 66 734.8 0 66 81 45 734.8 0 45 82 94 734.8 0 94 83 148 734.8 0 148 84 304 734.8 0 304 85 262 734.8 0 262 86 95 734.8 0 95 87 80 734.8 0 80 88 400 734.8 0 400 89 292 734.8 0 292 90 563 734.8 0 563 91 261 734.8 0 261 92 399 734.8 0 399 93 112 734.8 0 112 94 152 734.8 0 152 95 207 734.8 0 207 96 143 734.8 0 143 97 76 734.8 0 76 98 78 734.8 0 78 99 98 734.8 0 98 100 165 734.8 0 165 101 289 734.8 0 289 102 179 734.8 0 179 103 140 734.8 0 140 104 224 734.8 0 224 105 122 734.8 0 122 106 92 734.8 0 92 107 86 734.8 0 86 108 133 734.8 0 133 109 187 734.8 0 187 110 98 734.8 0 98 111 78 734.8 0 78 112 119 734.8 0 119 113 124 734.8 0 124 114 93 734.8 0 93 115 237 734.8 0 237 116 199 734.8 0 199 117 152 734.8 0 152 118 76 734.8 0 76 119 84 734.8 0 84 120 105 734.8 0 105 121 231 734.8 0 231 122 822 734.8 0 822 123 721 734.8 0 721 124 263 734.8 0 263 125 105 734.8 0 105 126 133 734.8 0 133 127 151 734.8 0 151 128 124 734.8 0 124 129 116 734.8 0 116 130 199 734.8 0 199 131 235 734.8 0 235 132 168 734.8 0 168 133 134 734.8 0 134 134 220 734.8 0 220 135 162 734.8 0 162 136 252 734.8 0 252 137 157 734.8 0 157 138 158 734.8 0 158 139 135 734.8 0 135 140 260 734.8 0 260 141 958 734.8 0 958 142 866 734.8 0 866 143 427 734.8 0 427 144 146 734.8 0 146 145 137 734.8 0 137 146 205 734.8 0 205 147 324 734.8 0 324 148 723 734.8 0 723 149 303 734.8 0 303 150 265 734.8 0 265 151 710 734.8 0 710 152 2753 734.8 0 2753 153 491 734.8 0 491 154 366 734.8 0 366 155 339 734.8 0 339 156 302 734.8 0 302 157 727 734.8 0 727 158 880 734.8 0 880 159 393 734.8 0 393 160 260 734.8 0 260 161 296 734.8 0 296 162 444 734.8 0 444 163 301 734.8 0 301 164 167 734.8 0 167 165 167 734.8 0 167 166 255 734.8 0 255 167 308 734.8 0 308 168 259 734.8 0 259 169 264 734.8 0 264 170 441 734.8 0 441 171 4132 734.8 0 4132 172 208 734.8 0 208 173 75 734.8 0 75 174 137 734.8 0 137 175 304 734.8 0 304 176 223 734.8 0 223 177 205 734.8 0 205 178 145 734.8 0 145 179 141 734.8 0 141 180 147 734.8 0 147 181 162 734.8 0 162 182 463 734.8 0 463 183 655 734.8 0 655 184 383 734.8 0 383 185 219 734.8 0 219 186 282 734.8 0 282 187 344 734.8 0 344 188 288 734.8 0 288 189 232 734.8 0 232 190 165 734.8 0 165 191 155 734.8 0 155 192 170 734.8 0 170 193 157 734.8 0 157 194 118 734.8 0 118 195 200 734.8 0 200 196 448 734.8 0 448 197 216 734.8 0 216 198 218 734.8 0 218 199 128 734.8 0 128 200 122 734.8 0 122 201 150 734.8 0 150 202 229 734.8 0 229 203 299 734.8 0 299 204 242 734.8 0 242 205 220 734.8 0 220 206 264 734.8 0 264 207 269 734.8 0 269 208 422 734.8 0 422 209 777 734.8 0 777 210 960 734.8 0 960 211 762 734.8 0 762 212 805 734.8 0 805 213 12296 734.8 0 12296 214 21351 734.8 0 21351 215 3991 734.8 0 3991 216 458 734.8 0 458 217 237 734.8 0 237 218 205 734.8 0 205 219 196 734.8 0 196 220 221 734.8 0 221 221 1311 734.8 0 1311 222 1530 734.8 0 1530 223 1013 734.8 0 1013 224 295 734.8 0 295 225 190 734.8 0 190 226 316 734.8 0 316 227 246 734.8 0 246 228 209 734.8 0 209 229 162 734.8 0 162 230 137 734.8 0 137 231 243 734.8 0 243 232 228 734.8 0 228 233 256 734.8 0 256 234 294 734.8 0 294 235 320 734.8 0 320 236 248 734.8 0 248 237 275 734.8 0 275 238 205 734.8 0 205 239 188 734.8 0 188 240 174 734.8 0 174 241 137 734.8 0 137 242 224 734.8 0 224 243 333 734.8 0 333 244 648 734.8 0 648 245 426 734.8 0 426 246 233 734.8 0 233 247 214 734.8 0 214 248 130 734.8 0 130 249 65 734.8 0 65 250 51 734.8 0 51 251 133 734.8 0 133 252 281 734.8 0 281 253 381 734.8 0 381 254 365 734.8 0 365 255 313 734.8 0 313 256 283 734.8 0 283 257 929 734.8 0 929 258 1049 734.8 0 1049 259 424 734.8 0 424 260 179 734.8 0 179 261 229 734.8 0 229 262 267 734.8 0 267 263 342 734.8 0 342 264 367 734.8 0 367 265 356 734.8 0 356 266 261 734.8 0 261 267 292 734.8 0 292 268 180 734.8 0 180 269 113 734.8 0 113 270 192 734.8 0 192 271 405 734.8 0 405 272 562 734.8 0 562 273 430 734.8 0 430 274 262 734.8 0 262 275 270 734.8 0 270 276 273 734.8 0 273 277 291 734.8 0 291 278 276 734.8 0 276 279 668 734.8 0 668 280 173 734.8 0 173 281 269 734.8 0 269 282 368 734.8 0 368 283 307 734.8 0 307 284 645 734.8 0 645 285 604 734.8 0 604 286 452 734.8 0 452 287 193 734.8 0 193 288 81 734.8 0 81 289 97 734.8 0 97 290 135 734.8 0 135 291 374 734.8 0 374 292 442 734.8 0 442 293 246 734.8 0 246 294 269 734.8 0 269 295 234 734.8 0 234 296 83 734.8 0 83 297 110 734.8 0 110 298 138 734.8 0 138 299 212 734.8 0 212 300 196 734.8 0 196 301 247 734.8 0 247 Finished in 4.19 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 95,766 Reads with adapters: 50,982 (53.2%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 50,975 (53.2%) Total basepairs processed: 22,776,703 bp Total written (filtered): 13,952,796 bp (61.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 50982 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 1 5 18 17 0.0 3 0 0 16 1 19 446 0.0 3 0 433 12 1 20 50216 0.0 4 49571 611 32 0 2 21 108 0.0 4 15 92 0 0 1 22 19 0.0 4 8 0 10 0 1 23 23 0.0 4 2 0 0 18 3 27 4 0.0 4 0 0 0 1 3 28 1 0.0 4 0 0 0 0 1 31 1 0.0 4 1 50 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 1 74 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 1 110 2 0.0 4 0 0 0 1 1 111 26 0.0 4 0 0 0 25 1 126 3 0.0 4 0 0 0 0 3 127 95 0.0 4 0 0 0 0 95 153 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.72 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 50,975 Reads with adapters: 621 (1.2%) Reads written (passing filters): 50,975 (100.0%) Total basepairs processed: 13,952,796 bp Total written (filtered): 13,944,572 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 621 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 1.8% G: 95.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 796.5 0 7 4 3 199.1 0 3 5 1 49.8 0 1 10 2 0.0 1 0 2 11 592 0.0 1 4 588 12 9 0.0 1 0 9 210 4 0.0 1 3 1 211 1 0.0 1 0 1 225 1 0.0 1 0 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.