This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.63 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 420,238 (14.0%) Reads that were too short: 42,052 (1.4%) Reads written (passing filters): 378,186 (12.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 97,223,676 bp (10.7%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 420238 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3220 734.8 0 3220 7 220767 734.8 0 220767 8 912 734.8 0 912 9 44411 734.8 0 44411 10 174 734.8 0 174 11 82 734.8 0 82 12 69 734.8 0 69 13 60 734.8 0 60 14 59 734.8 0 59 15 64 734.8 0 64 16 60 734.8 0 60 17 92 734.8 0 92 18 81 734.8 0 81 19 67 734.8 0 67 20 76 734.8 0 76 21 226 734.8 0 226 22 161 734.8 0 161 23 100 734.8 0 100 24 716 734.8 0 716 25 527 734.8 0 527 26 108 734.8 0 108 27 71 734.8 0 71 28 69 734.8 0 69 29 69 734.8 0 69 30 64 734.8 0 64 31 72 734.8 0 72 32 82 734.8 0 82 33 72 734.8 0 72 34 72 734.8 0 72 35 63 734.8 0 63 36 64 734.8 0 64 37 65 734.8 0 65 38 52 734.8 0 52 39 71 734.8 0 71 40 72 734.8 0 72 41 79 734.8 0 79 42 61 734.8 0 61 43 58 734.8 0 58 44 56 734.8 0 56 45 63 734.8 0 63 46 65 734.8 0 65 47 77 734.8 0 77 48 49 734.8 0 49 49 62 734.8 0 62 50 59 734.8 0 59 51 65 734.8 0 65 52 52 734.8 0 52 53 59 734.8 0 59 54 90 734.8 0 90 55 165 734.8 0 165 56 74 734.8 0 74 57 74 734.8 0 74 58 61 734.8 0 61 59 72 734.8 0 72 60 56 734.8 0 56 61 361 734.8 0 361 62 570 734.8 0 570 63 104 734.8 0 104 64 74 734.8 0 74 65 102 734.8 0 102 66 365 734.8 0 365 67 94 734.8 0 94 68 75 734.8 0 75 69 246 734.8 0 246 70 210 734.8 0 210 71 240 734.8 0 240 72 164 734.8 0 164 73 217 734.8 0 217 74 183 734.8 0 183 75 114 734.8 0 114 76 202 734.8 0 202 77 275 734.8 0 275 78 375 734.8 0 375 79 304 734.8 0 304 80 563 734.8 0 563 81 350 734.8 0 350 82 868 734.8 0 868 83 1824 734.8 0 1824 84 1077 734.8 0 1077 85 493 734.8 0 493 86 558 734.8 0 558 87 240 734.8 0 240 88 187 734.8 0 187 89 650 734.8 0 650 90 239 734.8 0 239 91 281 734.8 0 281 92 659 734.8 0 659 93 338 734.8 0 338 94 226 734.8 0 226 95 410 734.8 0 410 96 178 734.8 0 178 97 183 734.8 0 183 98 193 734.8 0 193 99 334 734.8 0 334 100 191 734.8 0 191 101 260 734.8 0 260 102 213 734.8 0 213 103 174 734.8 0 174 104 135 734.8 0 135 105 189 734.8 0 189 106 167 734.8 0 167 107 226 734.8 0 226 108 261 734.8 0 261 109 360 734.8 0 360 110 480 734.8 0 480 111 589 734.8 0 589 112 7233 734.8 0 7233 113 16087 734.8 0 16087 114 1323 734.8 0 1323 115 270 734.8 0 270 116 272 734.8 0 272 117 402 734.8 0 402 118 497 734.8 0 497 119 638 734.8 0 638 120 998 734.8 0 998 121 1288 734.8 0 1288 122 480 734.8 0 480 123 327 734.8 0 327 124 361 734.8 0 361 125 490 734.8 0 490 126 1348 734.8 0 1348 127 353 734.8 0 353 128 329 734.8 0 329 129 286 734.8 0 286 130 363 734.8 0 363 131 357 734.8 0 357 132 353 734.8 0 353 133 300 734.8 0 300 134 329 734.8 0 329 135 340 734.8 0 340 136 469 734.8 0 469 137 378 734.8 0 378 138 312 734.8 0 312 139 438 734.8 0 438 140 384 734.8 0 384 141 398 734.8 0 398 142 510 734.8 0 510 143 505 734.8 0 505 144 473 734.8 0 473 145 427 734.8 0 427 146 400 734.8 0 400 147 1401 734.8 0 1401 148 2027 734.8 0 2027 149 1242 734.8 0 1242 150 695 734.8 0 695 151 702 734.8 0 702 152 698 734.8 0 698 153 380 734.8 0 380 154 459 734.8 0 459 155 384 734.8 0 384 156 303 734.8 0 303 157 398 734.8 0 398 158 347 734.8 0 347 159 348 734.8 0 348 160 326 734.8 0 326 161 332 734.8 0 332 162 358 734.8 0 358 163 534 734.8 0 534 164 458 734.8 0 458 165 469 734.8 0 469 166 875 734.8 0 875 167 1045 734.8 0 1045 168 507 734.8 0 507 169 528 734.8 0 528 170 600 734.8 0 600 171 1486 734.8 0 1486 172 1956 734.8 0 1956 173 1207 734.8 0 1207 174 946 734.8 0 946 175 592 734.8 0 592 176 494 734.8 0 494 177 447 734.8 0 447 178 473 734.8 0 473 179 402 734.8 0 402 180 411 734.8 0 411 181 415 734.8 0 415 182 437 734.8 0 437 183 857 734.8 0 857 184 1941 734.8 0 1941 185 614 734.8 0 614 186 424 734.8 0 424 187 528 734.8 0 528 188 457 734.8 0 457 189 498 734.8 0 498 190 686 734.8 0 686 191 5923 734.8 0 5923 192 3976 734.8 0 3976 193 1423 734.8 0 1423 194 837 734.8 0 837 195 521 734.8 0 521 196 330 734.8 0 330 197 553 734.8 0 553 198 566 734.8 0 566 199 982 734.8 0 982 200 632 734.8 0 632 201 280 734.8 0 280 202 320 734.8 0 320 203 405 734.8 0 405 204 375 734.8 0 375 205 353 734.8 0 353 206 315 734.8 0 315 207 430 734.8 0 430 208 334 734.8 0 334 209 344 734.8 0 344 210 303 734.8 0 303 211 359 734.8 0 359 212 387 734.8 0 387 213 363 734.8 0 363 214 356 734.8 0 356 215 405 734.8 0 405 216 453 734.8 0 453 217 378 734.8 0 378 218 465 734.8 0 465 219 573 734.8 0 573 220 409 734.8 0 409 221 225 734.8 0 225 222 255 734.8 0 255 223 392 734.8 0 392 224 398 734.8 0 398 225 383 734.8 0 383 226 507 734.8 0 507 227 730 734.8 0 730 228 364 734.8 0 364 229 744 734.8 0 744 230 366 734.8 0 366 231 315 734.8 0 315 232 333 734.8 0 333 233 302 734.8 0 302 234 354 734.8 0 354 235 435 734.8 0 435 236 387 734.8 0 387 237 194 734.8 0 194 238 267 734.8 0 267 239 409 734.8 0 409 240 423 734.8 0 423 241 475 734.8 0 475 242 446 734.8 0 446 243 538 734.8 0 538 244 378 734.8 0 378 245 445 734.8 0 445 246 565 734.8 0 565 247 412 734.8 0 412 248 234 734.8 0 234 249 211 734.8 0 211 250 199 734.8 0 199 251 646 734.8 0 646 252 1966 734.8 0 1966 253 562 734.8 0 562 254 338 734.8 0 338 255 346 734.8 0 346 256 377 734.8 0 377 257 312 734.8 0 312 258 186 734.8 0 186 259 207 734.8 0 207 260 197 734.8 0 197 261 270 734.8 0 270 262 479 734.8 0 479 263 470 734.8 0 470 264 481 734.8 0 481 265 440 734.8 0 440 266 321 734.8 0 321 267 763 734.8 0 763 268 913 734.8 0 913 269 356 734.8 0 356 270 297 734.8 0 297 271 365 734.8 0 365 272 422 734.8 0 422 273 267 734.8 0 267 274 338 734.8 0 338 275 369 734.8 0 369 276 230 734.8 0 230 277 322 734.8 0 322 278 731 734.8 0 731 279 447 734.8 0 447 280 330 734.8 0 330 281 239 734.8 0 239 282 331 734.8 0 331 283 731 734.8 0 731 284 552 734.8 0 552 285 366 734.8 0 366 286 425 734.8 0 425 287 314 734.8 0 314 288 239 734.8 0 239 289 420 734.8 0 420 290 552 734.8 0 552 291 570 734.8 0 570 292 643 734.8 0 643 293 661 734.8 0 661 294 402 734.8 0 402 295 368 734.8 0 368 296 461 734.8 0 461 297 670 734.8 0 670 298 511 734.8 0 511 299 358 734.8 0 358 300 374 734.8 0 374 301 104 734.8 0 104 Finished in 15.63 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 378,186 Reads with adapters: 46,216 (12.2%) Reads that were too short: 367 (0.1%) Reads written (passing filters): 45,849 (12.1%) Total basepairs processed: 97,223,676 bp Total written (filtered): 12,558,166 bp (12.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46216 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 14 0.0 3 0 0 14 16 378 0.0 3 0 336 26 16 17 44810 0.0 3 43671 997 109 33 18 209 0.0 3 17 150 9 33 19 15 0.0 3 0 0 11 4 20 75 0.0 3 0 0 0 75 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 94 0.0 3 0 0 1 93 25 1 0.0 3 0 0 0 1 26 9 0.0 3 0 0 0 9 27 13 0.0 3 0 0 1 12 28 4 0.0 3 0 0 0 4 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 5 0.0 3 0 0 0 5 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 1 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 3 0.0 3 0 0 0 3 51 11 0.0 3 0 0 0 11 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 54 4 0.0 3 0 0 0 4 55 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 66 13 0.0 3 0 0 0 13 67 4 0.0 3 0 0 0 4 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 4 0.0 3 0 0 0 4 78 4 0.0 3 0 0 0 4 79 10 0.0 3 0 0 0 10 81 3 0.0 3 0 0 0 3 82 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 4 0.0 3 0 0 0 4 97 1 0.0 3 0 0 0 1 98 5 0.0 3 0 0 1 4 99 1 0.0 3 0 0 0 1 101 5 0.0 3 0 0 0 5 102 6 0.0 3 0 0 0 6 103 3 0.0 3 0 0 0 3 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 1 109 6 0.0 3 0 0 0 6 110 11 0.0 3 0 0 0 11 111 3 0.0 3 0 0 0 3 112 21 0.0 3 0 0 1 20 113 8 0.0 3 0 0 1 7 114 2 0.0 3 0 0 0 2 115 25 0.0 3 0 0 1 24 116 3 0.0 3 0 0 0 3 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 1 0 1 120 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 122 8 0.0 3 0 0 1 7 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 136 28 0.0 3 0 0 0 28 137 7 0.0 3 0 0 1 6 139 25 0.0 3 0 0 2 23 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 4 0.0 3 0 0 0 4 147 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 150 4 0.0 3 0 0 0 4 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 162 6 0.0 3 0 0 0 6 163 1 0.0 3 0 0 0 1 167 9 0.0 3 0 0 1 8 168 2 0.0 3 0 0 0 2 169 2 0.0 3 0 0 0 2 170 9 0.0 3 0 0 0 9 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 39 0.0 3 0 0 1 38 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 32 0.0 3 0 0 0 32 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 7 0.0 3 0 0 0 7 218 1 0.0 3 0 0 1 219 1 0.0 3 0 0 0 1 222 3 0.0 3 0 0 0 3 224 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 226 3 0.0 3 0 0 0 3 227 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 259 2 0.0 3 0 0 0 2 260 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 281 30 0.0 3 0 0 0 30 282 47 0.0 3 0 0 1 46 283 10 0.0 3 0 0 0 10 286 3 0.0 3 0 0 0 3 288 6 0.0 3 0 0 0 6 289 5 0.0 3 0 0 0 5 290 4 0.0 3 0 0 0 4 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.61 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 45,849 Reads with adapters: 1,819 (4.0%) Reads written (passing filters): 45,849 (100.0%) Total basepairs processed: 12,558,166 bp Total written (filtered): 12,552,683 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1819 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.1% C: 5.0% G: 10.1% T: 3.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1796 716.4 0 1796 4 21 179.1 0 21 5 1 44.8 0 1 6 1 11.2 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.