This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.77 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 292,143 (9.7%) Reads that were too short: 8,173 (0.3%) Reads written (passing filters): 283,970 (9.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 70,503,473 bp (7.8%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 292143 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1100 734.8 0 1100 7 1114 734.8 0 1114 8 60170 734.8 0 60170 9 39537 734.8 0 39537 10 100 734.8 0 100 11 28 734.8 0 28 12 46 734.8 0 46 13 36 734.8 0 36 14 35 734.8 0 35 15 28 734.8 0 28 16 36 734.8 0 36 17 40 734.8 0 40 18 38 734.8 0 38 19 35 734.8 0 35 20 42 734.8 0 42 21 31 734.8 0 31 22 61 734.8 0 61 23 77 734.8 0 77 24 40 734.8 0 40 25 46 734.8 0 46 26 44 734.8 0 44 27 43 734.8 0 43 28 32 734.8 0 32 29 1099 734.8 0 1099 30 306 734.8 0 306 31 40 734.8 0 40 32 46 734.8 0 46 33 48 734.8 0 48 34 70 734.8 0 70 35 45 734.8 0 45 36 60 734.8 0 60 37 53 734.8 0 53 38 115 734.8 0 115 39 58 734.8 0 58 40 75 734.8 0 75 41 36 734.8 0 36 42 72 734.8 0 72 43 52 734.8 0 52 44 115 734.8 0 115 45 131 734.8 0 131 46 154 734.8 0 154 47 196 734.8 0 196 48 438 734.8 0 438 49 1207 734.8 0 1207 50 544 734.8 0 544 51 746 734.8 0 746 52 552 734.8 0 552 53 378 734.8 0 378 54 379 734.8 0 379 55 644 734.8 0 644 56 544 734.8 0 544 57 737 734.8 0 737 58 675 734.8 0 675 59 1088 734.8 0 1088 60 1163 734.8 0 1163 61 2045 734.8 0 2045 62 59569 734.8 0 59569 63 59696 734.8 0 59696 64 12816 734.8 0 12816 65 176 734.8 0 176 66 56 734.8 0 56 67 33 734.8 0 33 68 26 734.8 0 26 69 34 734.8 0 34 70 52 734.8 0 52 71 104 734.8 0 104 72 112 734.8 0 112 73 75 734.8 0 75 74 93 734.8 0 93 75 94 734.8 0 94 76 1356 734.8 0 1356 77 3174 734.8 0 3174 78 270 734.8 0 270 79 76 734.8 0 76 80 35 734.8 0 35 81 27 734.8 0 27 82 29 734.8 0 29 83 35 734.8 0 35 84 37 734.8 0 37 85 46 734.8 0 46 86 33 734.8 0 33 87 34 734.8 0 34 88 39 734.8 0 39 89 19 734.8 0 19 90 22 734.8 0 22 91 34 734.8 0 34 92 31 734.8 0 31 93 55 734.8 0 55 94 48 734.8 0 48 95 42 734.8 0 42 96 24 734.8 0 24 97 34 734.8 0 34 98 19 734.8 0 19 99 55 734.8 0 55 100 31 734.8 0 31 101 40 734.8 0 40 102 37 734.8 0 37 103 28 734.8 0 28 104 27 734.8 0 27 105 56 734.8 0 56 106 36 734.8 0 36 107 87 734.8 0 87 108 102 734.8 0 102 109 46 734.8 0 46 110 32 734.8 0 32 111 44 734.8 0 44 112 53 734.8 0 53 113 41 734.8 0 41 114 36 734.8 0 36 115 38 734.8 0 38 116 35 734.8 0 35 117 40 734.8 0 40 118 28 734.8 0 28 119 41 734.8 0 41 120 41 734.8 0 41 121 42 734.8 0 42 122 44 734.8 0 44 123 38 734.8 0 38 124 57 734.8 0 57 125 43 734.8 0 43 126 58 734.8 0 58 127 73 734.8 0 73 128 59 734.8 0 59 129 63 734.8 0 63 130 57 734.8 0 57 131 50 734.8 0 50 132 38 734.8 0 38 133 65 734.8 0 65 134 51 734.8 0 51 135 46 734.8 0 46 136 50 734.8 0 50 137 59 734.8 0 59 138 63 734.8 0 63 139 52 734.8 0 52 140 61 734.8 0 61 141 85 734.8 0 85 142 187 734.8 0 187 143 170 734.8 0 170 144 1870 734.8 0 1870 145 4007 734.8 0 4007 146 513 734.8 0 513 147 106 734.8 0 106 148 41 734.8 0 41 149 92 734.8 0 92 150 157 734.8 0 157 151 90 734.8 0 90 152 90 734.8 0 90 153 54 734.8 0 54 154 61 734.8 0 61 155 66 734.8 0 66 156 601 734.8 0 601 157 703 734.8 0 703 158 135 734.8 0 135 159 64 734.8 0 64 160 96 734.8 0 96 161 106 734.8 0 106 162 167 734.8 0 167 163 255 734.8 0 255 164 3796 734.8 0 3796 165 4690 734.8 0 4690 166 726 734.8 0 726 167 98 734.8 0 98 168 124 734.8 0 124 169 617 734.8 0 617 170 974 734.8 0 974 171 754 734.8 0 754 172 621 734.8 0 621 173 363 734.8 0 363 174 135 734.8 0 135 175 53 734.8 0 53 176 46 734.8 0 46 177 43 734.8 0 43 178 54 734.8 0 54 179 108 734.8 0 108 180 85 734.8 0 85 181 102 734.8 0 102 182 92 734.8 0 92 183 74 734.8 0 74 184 100 734.8 0 100 185 136 734.8 0 136 186 151 734.8 0 151 187 71 734.8 0 71 188 98 734.8 0 98 189 696 734.8 0 696 190 1106 734.8 0 1106 191 598 734.8 0 598 192 157 734.8 0 157 193 45 734.8 0 45 194 45 734.8 0 45 195 37 734.8 0 37 196 47 734.8 0 47 197 65 734.8 0 65 198 47 734.8 0 47 199 55 734.8 0 55 200 42 734.8 0 42 201 35 734.8 0 35 202 46 734.8 0 46 203 51 734.8 0 51 204 63 734.8 0 63 205 51 734.8 0 51 206 75 734.8 0 75 207 66 734.8 0 66 208 65 734.8 0 65 209 69 734.8 0 69 210 43 734.8 0 43 211 50 734.8 0 50 212 50 734.8 0 50 213 58 734.8 0 58 214 64 734.8 0 64 215 63 734.8 0 63 216 153 734.8 0 153 217 163 734.8 0 163 218 141 734.8 0 141 219 52 734.8 0 52 220 56 734.8 0 56 221 46 734.8 0 46 222 36 734.8 0 36 223 57 734.8 0 57 224 50 734.8 0 50 225 71 734.8 0 71 226 104 734.8 0 104 227 113 734.8 0 113 228 96 734.8 0 96 229 272 734.8 0 272 230 134 734.8 0 134 231 86 734.8 0 86 232 64 734.8 0 64 233 48 734.8 0 48 234 52 734.8 0 52 235 44 734.8 0 44 236 77 734.8 0 77 237 85 734.8 0 85 238 60 734.8 0 60 239 58 734.8 0 58 240 50 734.8 0 50 241 48 734.8 0 48 242 38 734.8 0 38 243 64 734.8 0 64 244 66 734.8 0 66 245 98 734.8 0 98 246 63 734.8 0 63 247 60 734.8 0 60 248 49 734.8 0 49 249 59 734.8 0 59 250 43 734.8 0 43 251 29 734.8 0 29 252 31 734.8 0 31 253 33 734.8 0 33 254 69 734.8 0 69 255 53 734.8 0 53 256 59 734.8 0 59 257 72 734.8 0 72 258 54 734.8 0 54 259 80 734.8 0 80 260 248 734.8 0 248 261 261 734.8 0 261 262 127 734.8 0 127 263 69 734.8 0 69 264 73 734.8 0 73 265 84 734.8 0 84 266 106 734.8 0 106 267 92 734.8 0 92 268 60 734.8 0 60 269 44 734.8 0 44 270 49 734.8 0 49 271 38 734.8 0 38 272 43 734.8 0 43 273 51 734.8 0 51 274 58 734.8 0 58 275 65 734.8 0 65 276 79 734.8 0 79 277 103 734.8 0 103 278 140 734.8 0 140 279 89 734.8 0 89 280 141 734.8 0 141 281 107 734.8 0 107 282 50 734.8 0 50 283 52 734.8 0 52 284 52 734.8 0 52 285 85 734.8 0 85 286 89 734.8 0 89 287 236 734.8 0 236 288 586 734.8 0 586 289 225 734.8 0 225 290 41 734.8 0 41 291 40 734.8 0 40 292 32 734.8 0 32 293 58 734.8 0 58 294 62 734.8 0 62 295 68 734.8 0 68 296 98 734.8 0 98 297 67 734.8 0 67 298 53 734.8 0 53 299 28 734.8 0 28 300 39 734.8 0 39 301 35 734.8 0 35 Finished in 13.36 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 283,970 Reads with adapters: 53,626 (18.9%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 53,603 (18.9%) Total basepairs processed: 70,503,473 bp Total written (filtered): 13,277,270 bp (18.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53626 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 23 0.0 3 0 0 23 19 529 0.0 3 0 520 5 1 3 20 40509 0.0 4 39929 563 13 2 2 21 98 0.0 4 15 78 1 0 4 22 10 0.0 4 1 0 8 1 23 15 0.0 4 0 0 0 14 1 24 3 0.0 4 0 0 0 0 3 34 2 0.0 4 0 0 0 0 2 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 43 1 0.0 4 1 69 1 0.0 4 0 0 0 0 1 70 14 0.0 4 0 0 0 0 14 71 9 0.0 4 0 0 0 0 9 72 9109 0.0 4 0 0 0 0 9109 87 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 6 0.0 4 0 0 0 0 6 126 45 0.0 4 0 0 0 0 45 127 3211 0.0 4 0 0 0 0 3211 128 9 0.0 4 0 0 0 0 9 132 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 3 0.0 4 0 0 0 0 3 185 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 233 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.73 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 53,603 Reads with adapters: 1,768 (3.3%) Reads written (passing filters): 53,603 (100.0%) Total basepairs processed: 13,277,270 bp Total written (filtered): 13,108,543 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1768 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 32.7% C: 1.5% G: 64.1% T: 1.5% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 21 837.5 0 21 4 47 209.4 0 47 5 6 52.3 0 6 6 6 13.1 0 6 9 131 0.2 0 10 121 10 784 0.1 1 21 763 11 156 0.0 1 1 155 12 1 0.0 1 0 1 106 1 0.0 1 1 150 1 0.0 1 0 1 169 1 0.0 1 1 207 1 0.0 1 1 208 6 0.0 1 3 3 212 1 0.0 1 1 213 7 0.0 1 6 1 214 1 0.0 1 1 256 1 0.0 1 1 257 406 0.0 1 383 23 258 134 0.0 1 127 7 259 7 0.0 1 7 260 32 0.0 1 30 2 261 2 0.0 1 2 269 13 0.0 1 13 272 2 0.0 1 2