This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.79 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 329,492 (10.9%) Reads that were too short: 23,361 (0.8%) Reads written (passing filters): 306,131 (10.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 87,832,555 bp (9.7%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 329492 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1639 734.8 0 1639 7 2702 734.8 0 2702 8 280526 734.8 0 280526 9 3737 734.8 0 3737 10 74 734.8 0 74 11 60 734.8 0 60 12 75 734.8 0 75 13 64 734.8 0 64 14 63 734.8 0 63 15 64 734.8 0 64 16 81 734.8 0 81 17 55 734.8 0 55 18 74 734.8 0 74 19 76 734.8 0 76 20 72 734.8 0 72 21 87 734.8 0 87 22 60 734.8 0 60 23 58 734.8 0 58 24 70 734.8 0 70 25 64 734.8 0 64 26 69 734.8 0 69 27 68 734.8 0 68 28 47 734.8 0 47 29 78 734.8 0 78 30 88 734.8 0 88 31 56 734.8 0 56 32 53 734.8 0 53 33 65 734.8 0 65 34 83 734.8 0 83 35 76 734.8 0 76 36 69 734.8 0 69 37 72 734.8 0 72 38 54 734.8 0 54 39 50 734.8 0 50 40 78 734.8 0 78 41 76 734.8 0 76 42 67 734.8 0 67 43 49 734.8 0 49 44 75 734.8 0 75 45 112 734.8 0 112 46 67 734.8 0 67 47 74 734.8 0 74 48 55 734.8 0 55 49 69 734.8 0 69 50 62 734.8 0 62 51 48 734.8 0 48 52 85 734.8 0 85 53 77 734.8 0 77 54 61 734.8 0 61 55 63 734.8 0 63 56 68 734.8 0 68 57 53 734.8 0 53 58 63 734.8 0 63 59 114 734.8 0 114 60 142 734.8 0 142 61 96 734.8 0 96 62 87 734.8 0 87 63 72 734.8 0 72 64 61 734.8 0 61 65 62 734.8 0 62 66 54 734.8 0 54 67 67 734.8 0 67 68 84 734.8 0 84 69 71 734.8 0 71 70 100 734.8 0 100 71 82 734.8 0 82 72 103 734.8 0 103 73 65 734.8 0 65 74 53 734.8 0 53 75 70 734.8 0 70 76 117 734.8 0 117 77 223 734.8 0 223 78 179 734.8 0 179 79 84 734.8 0 84 80 94 734.8 0 94 81 106 734.8 0 106 82 97 734.8 0 97 83 80 734.8 0 80 84 97 734.8 0 97 85 102 734.8 0 102 86 80 734.8 0 80 87 81 734.8 0 81 88 74 734.8 0 74 89 76 734.8 0 76 90 76 734.8 0 76 91 84 734.8 0 84 92 100 734.8 0 100 93 131 734.8 0 131 94 129 734.8 0 129 95 94 734.8 0 94 96 89 734.8 0 89 97 89 734.8 0 89 98 140 734.8 0 140 99 80 734.8 0 80 100 86 734.8 0 86 101 63 734.8 0 63 102 69 734.8 0 69 103 77 734.8 0 77 104 72 734.8 0 72 105 68 734.8 0 68 106 97 734.8 0 97 107 67 734.8 0 67 108 50 734.8 0 50 109 64 734.8 0 64 110 83 734.8 0 83 111 77 734.8 0 77 112 108 734.8 0 108 113 104 734.8 0 104 114 91 734.8 0 91 115 79 734.8 0 79 116 95 734.8 0 95 117 114 734.8 0 114 118 102 734.8 0 102 119 77 734.8 0 77 120 75 734.8 0 75 121 90 734.8 0 90 122 79 734.8 0 79 123 52 734.8 0 52 124 73 734.8 0 73 125 77 734.8 0 77 126 86 734.8 0 86 127 92 734.8 0 92 128 124 734.8 0 124 129 111 734.8 0 111 130 142 734.8 0 142 131 102 734.8 0 102 132 131 734.8 0 131 133 130 734.8 0 130 134 117 734.8 0 117 135 94 734.8 0 94 136 90 734.8 0 90 137 79 734.8 0 79 138 124 734.8 0 124 139 135 734.8 0 135 140 91 734.8 0 91 141 88 734.8 0 88 142 107 734.8 0 107 143 154 734.8 0 154 144 138 734.8 0 138 145 229 734.8 0 229 146 78 734.8 0 78 147 110 734.8 0 110 148 95 734.8 0 95 149 95 734.8 0 95 150 84 734.8 0 84 151 118 734.8 0 118 152 86 734.8 0 86 153 97 734.8 0 97 154 97 734.8 0 97 155 114 734.8 0 114 156 105 734.8 0 105 157 156 734.8 0 156 158 115 734.8 0 115 159 99 734.8 0 99 160 106 734.8 0 106 161 78 734.8 0 78 162 113 734.8 0 113 163 89 734.8 0 89 164 94 734.8 0 94 165 91 734.8 0 91 166 88 734.8 0 88 167 85 734.8 0 85 168 83 734.8 0 83 169 83 734.8 0 83 170 96 734.8 0 96 171 105 734.8 0 105 172 91 734.8 0 91 173 79 734.8 0 79 174 88 734.8 0 88 175 94 734.8 0 94 176 83 734.8 0 83 177 92 734.8 0 92 178 73 734.8 0 73 179 94 734.8 0 94 180 79 734.8 0 79 181 80 734.8 0 80 182 73 734.8 0 73 183 88 734.8 0 88 184 105 734.8 0 105 185 73 734.8 0 73 186 98 734.8 0 98 187 102 734.8 0 102 188 92 734.8 0 92 189 83 734.8 0 83 190 112 734.8 0 112 191 210 734.8 0 210 192 143 734.8 0 143 193 109 734.8 0 109 194 120 734.8 0 120 195 95 734.8 0 95 196 97 734.8 0 97 197 270 734.8 0 270 198 289 734.8 0 289 199 119 734.8 0 119 200 84 734.8 0 84 201 87 734.8 0 87 202 53 734.8 0 53 203 71 734.8 0 71 204 99 734.8 0 99 205 120 734.8 0 120 206 92 734.8 0 92 207 88 734.8 0 88 208 69 734.8 0 69 209 75 734.8 0 75 210 100 734.8 0 100 211 103 734.8 0 103 212 96 734.8 0 96 213 110 734.8 0 110 214 101 734.8 0 101 215 118 734.8 0 118 216 84 734.8 0 84 217 82 734.8 0 82 218 97 734.8 0 97 219 91 734.8 0 91 220 86 734.8 0 86 221 85 734.8 0 85 222 87 734.8 0 87 223 113 734.8 0 113 224 357 734.8 0 357 225 98 734.8 0 98 226 86 734.8 0 86 227 95 734.8 0 95 228 74 734.8 0 74 229 67 734.8 0 67 230 101 734.8 0 101 231 131 734.8 0 131 232 137 734.8 0 137 233 104 734.8 0 104 234 108 734.8 0 108 235 89 734.8 0 89 236 97 734.8 0 97 237 111 734.8 0 111 238 126 734.8 0 126 239 110 734.8 0 110 240 133 734.8 0 133 241 104 734.8 0 104 242 90 734.8 0 90 243 91 734.8 0 91 244 86 734.8 0 86 245 125 734.8 0 125 246 154 734.8 0 154 247 117 734.8 0 117 248 83 734.8 0 83 249 89 734.8 0 89 250 89 734.8 0 89 251 55 734.8 0 55 252 61 734.8 0 61 253 110 734.8 0 110 254 161 734.8 0 161 255 130 734.8 0 130 256 263 734.8 0 263 257 321 734.8 0 321 258 195 734.8 0 195 259 94 734.8 0 94 260 97 734.8 0 97 261 93 734.8 0 93 262 93 734.8 0 93 263 102 734.8 0 102 264 125 734.8 0 125 265 533 734.8 0 533 266 449 734.8 0 449 267 279 734.8 0 279 268 203 734.8 0 203 269 79 734.8 0 79 270 70 734.8 0 70 271 69 734.8 0 69 272 109 734.8 0 109 273 108 734.8 0 108 274 85 734.8 0 85 275 107 734.8 0 107 276 76 734.8 0 76 277 88 734.8 0 88 278 76 734.8 0 76 279 96 734.8 0 96 280 101 734.8 0 101 281 241 734.8 0 241 282 130 734.8 0 130 283 113 734.8 0 113 284 158 734.8 0 158 285 184 734.8 0 184 286 137 734.8 0 137 287 150 734.8 0 150 288 192 734.8 0 192 289 221 734.8 0 221 290 235 734.8 0 235 291 277 734.8 0 277 292 356 734.8 0 356 293 509 734.8 0 509 294 406 734.8 0 406 295 394 734.8 0 394 296 415 734.8 0 415 297 462 734.8 0 462 298 2314 734.8 0 2314 299 2141 734.8 0 2141 300 4250 734.8 0 4250 301 576 734.8 0 576 Finished in 12.31 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 306,131 Reads with adapters: 284,145 (92.8%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 284,129 (92.8%) Total basepairs processed: 87,832,555 bp Total written (filtered): 78,419,352 bp (89.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 284145 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 17 0.0 2 0 0 0 17 15 116 0.0 3 0 0 87 29 16 1820 0.0 3 0 1657 114 49 17 280556 0.0 3 272296 7324 789 147 18 867 0.0 3 34 696 43 94 19 332 0.0 3 1 0 220 111 20 372 0.0 3 0 0 0 372 21 3 0.0 3 2 0 1 22 1 0.0 3 0 0 1 23 4 0.0 3 2 0 0 2 24 1 0.0 3 1 28 2 0.0 3 1 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 1 32 1 0.0 3 1 34 1 0.0 3 1 36 1 0.0 3 1 37 1 0.0 3 1 39 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 183 3 0.0 3 0 0 0 3 186 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 1 1 188 1 0.0 3 0 0 0 1 189 6 0.0 3 0 0 0 6 192 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.98 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 284,129 Reads with adapters: 12,756 (4.5%) Reads written (passing filters): 284,129 (100.0%) Total basepairs processed: 78,419,352 bp Total written (filtered): 78,380,867 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 12756 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.1% C: 2.9% G: 52.4% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12575 4439.5 0 12575 4 159 1109.9 0 159 5 17 277.5 0 17 6 2 69.4 0 2 7 2 17.3 0 2 13 1 0.0 1 0 1