This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.40 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 333,200 (11.1%) Reads that were too short: 5,382 (0.2%) Reads written (passing filters): 327,818 (10.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 92,130,327 bp (10.2%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 333200 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3248 734.8 0 3248 7 7284 734.8 0 7284 8 284095 734.8 0 284095 9 424 734.8 0 424 10 46 734.8 0 46 11 37 734.8 0 37 12 47 734.8 0 47 13 22 734.8 0 22 14 35 734.8 0 35 15 36 734.8 0 36 16 49 734.8 0 49 17 37 734.8 0 37 18 51 734.8 0 51 19 114 734.8 0 114 20 68 734.8 0 68 21 62 734.8 0 62 22 43 734.8 0 43 23 48 734.8 0 48 24 33 734.8 0 33 25 46 734.8 0 46 26 61 734.8 0 61 27 55 734.8 0 55 28 35 734.8 0 35 29 41 734.8 0 41 30 39 734.8 0 39 31 39 734.8 0 39 32 42 734.8 0 42 33 38 734.8 0 38 34 56 734.8 0 56 35 55 734.8 0 55 36 48 734.8 0 48 37 42 734.8 0 42 38 54 734.8 0 54 39 63 734.8 0 63 40 50 734.8 0 50 41 49 734.8 0 49 42 37 734.8 0 37 43 42 734.8 0 42 44 42 734.8 0 42 45 63 734.8 0 63 46 49 734.8 0 49 47 32 734.8 0 32 48 46 734.8 0 46 49 58 734.8 0 58 50 39 734.8 0 39 51 51 734.8 0 51 52 55 734.8 0 55 53 55 734.8 0 55 54 28 734.8 0 28 55 36 734.8 0 36 56 34 734.8 0 34 57 52 734.8 0 52 58 50 734.8 0 50 59 47 734.8 0 47 60 54 734.8 0 54 61 49 734.8 0 49 62 40 734.8 0 40 63 38 734.8 0 38 64 54 734.8 0 54 65 35 734.8 0 35 66 48 734.8 0 48 67 31 734.8 0 31 68 31 734.8 0 31 69 43 734.8 0 43 70 44 734.8 0 44 71 55 734.8 0 55 72 41 734.8 0 41 73 52 734.8 0 52 74 49 734.8 0 49 75 39 734.8 0 39 76 183 734.8 0 183 77 88 734.8 0 88 78 45 734.8 0 45 79 40 734.8 0 40 80 34 734.8 0 34 81 39 734.8 0 39 82 44 734.8 0 44 83 38 734.8 0 38 84 38 734.8 0 38 85 39 734.8 0 39 86 34 734.8 0 34 87 37 734.8 0 37 88 39 734.8 0 39 89 31 734.8 0 31 90 36 734.8 0 36 91 43 734.8 0 43 92 46 734.8 0 46 93 90 734.8 0 90 94 104 734.8 0 104 95 56 734.8 0 56 96 169 734.8 0 169 97 214 734.8 0 214 98 104 734.8 0 104 99 38 734.8 0 38 100 42 734.8 0 42 101 32 734.8 0 32 102 30 734.8 0 30 103 34 734.8 0 34 104 31 734.8 0 31 105 32 734.8 0 32 106 38 734.8 0 38 107 37 734.8 0 37 108 58 734.8 0 58 109 38 734.8 0 38 110 35 734.8 0 35 111 34 734.8 0 34 112 39 734.8 0 39 113 28 734.8 0 28 114 89 734.8 0 89 115 53 734.8 0 53 116 75 734.8 0 75 117 90 734.8 0 90 118 38 734.8 0 38 119 73 734.8 0 73 120 72 734.8 0 72 121 62 734.8 0 62 122 71 734.8 0 71 123 81 734.8 0 81 124 72 734.8 0 72 125 112 734.8 0 112 126 118 734.8 0 118 127 207 734.8 0 207 128 216 734.8 0 216 129 518 734.8 0 518 130 13003 734.8 0 13003 131 4381 734.8 0 4381 132 558 734.8 0 558 133 160 734.8 0 160 134 52 734.8 0 52 135 39 734.8 0 39 136 40 734.8 0 40 137 43 734.8 0 43 138 45 734.8 0 45 139 31 734.8 0 31 140 29 734.8 0 29 141 32 734.8 0 32 142 37 734.8 0 37 143 65 734.8 0 65 144 89 734.8 0 89 145 56 734.8 0 56 146 23 734.8 0 23 147 32 734.8 0 32 148 29 734.8 0 29 149 38 734.8 0 38 150 36 734.8 0 36 151 35 734.8 0 35 152 32 734.8 0 32 153 36 734.8 0 36 154 49 734.8 0 49 155 23 734.8 0 23 156 30 734.8 0 30 157 40 734.8 0 40 158 55 734.8 0 55 159 39 734.8 0 39 160 26 734.8 0 26 161 28 734.8 0 28 162 34 734.8 0 34 163 33 734.8 0 33 164 59 734.8 0 59 165 65 734.8 0 65 166 70 734.8 0 70 167 95 734.8 0 95 168 151 734.8 0 151 169 2545 734.8 0 2545 170 1781 734.8 0 1781 171 504 734.8 0 504 172 28 734.8 0 28 173 26 734.8 0 26 174 41 734.8 0 41 175 35 734.8 0 35 176 25 734.8 0 25 177 21 734.8 0 21 178 32 734.8 0 32 179 37 734.8 0 37 180 33 734.8 0 33 181 32 734.8 0 32 182 31 734.8 0 31 183 30 734.8 0 30 184 28 734.8 0 28 185 43 734.8 0 43 186 32 734.8 0 32 187 37 734.8 0 37 188 26 734.8 0 26 189 34 734.8 0 34 190 40 734.8 0 40 191 38 734.8 0 38 192 65 734.8 0 65 193 70 734.8 0 70 194 48 734.8 0 48 195 32 734.8 0 32 196 26 734.8 0 26 197 35 734.8 0 35 198 49 734.8 0 49 199 33 734.8 0 33 200 40 734.8 0 40 201 28 734.8 0 28 202 45 734.8 0 45 203 100 734.8 0 100 204 76 734.8 0 76 205 73 734.8 0 73 206 31 734.8 0 31 207 33 734.8 0 33 208 30 734.8 0 30 209 37 734.8 0 37 210 34 734.8 0 34 211 40 734.8 0 40 212 25 734.8 0 25 213 45 734.8 0 45 214 39 734.8 0 39 215 48 734.8 0 48 216 30 734.8 0 30 217 55 734.8 0 55 218 53 734.8 0 53 219 30 734.8 0 30 220 33 734.8 0 33 221 53 734.8 0 53 222 119 734.8 0 119 223 165 734.8 0 165 224 64 734.8 0 64 225 30 734.8 0 30 226 52 734.8 0 52 227 47 734.8 0 47 228 45 734.8 0 45 229 31 734.8 0 31 230 36 734.8 0 36 231 52 734.8 0 52 232 107 734.8 0 107 233 38 734.8 0 38 234 43 734.8 0 43 235 81 734.8 0 81 236 70 734.8 0 70 237 327 734.8 0 327 238 53 734.8 0 53 239 32 734.8 0 32 240 29 734.8 0 29 241 49 734.8 0 49 242 45 734.8 0 45 243 32 734.8 0 32 244 29 734.8 0 29 245 46 734.8 0 46 246 46 734.8 0 46 247 37 734.8 0 37 248 18 734.8 0 18 249 21 734.8 0 21 250 21 734.8 0 21 251 28 734.8 0 28 252 34 734.8 0 34 253 47 734.8 0 47 254 37 734.8 0 37 255 31 734.8 0 31 256 64 734.8 0 64 257 64 734.8 0 64 258 28 734.8 0 28 259 36 734.8 0 36 260 21 734.8 0 21 261 35 734.8 0 35 262 47 734.8 0 47 263 35 734.8 0 35 264 48 734.8 0 48 265 28 734.8 0 28 266 33 734.8 0 33 267 41 734.8 0 41 268 23 734.8 0 23 269 37 734.8 0 37 270 51 734.8 0 51 271 197 734.8 0 197 272 244 734.8 0 244 273 234 734.8 0 234 274 122 734.8 0 122 275 28 734.8 0 28 276 43 734.8 0 43 277 43 734.8 0 43 278 60 734.8 0 60 279 46 734.8 0 46 280 40 734.8 0 40 281 25 734.8 0 25 282 33 734.8 0 33 283 39 734.8 0 39 284 27 734.8 0 27 285 40 734.8 0 40 286 42 734.8 0 42 287 32 734.8 0 32 288 47 734.8 0 47 289 50 734.8 0 50 290 46 734.8 0 46 291 33 734.8 0 33 292 35 734.8 0 35 293 42 734.8 0 42 294 29 734.8 0 29 295 32 734.8 0 32 296 90 734.8 0 90 297 101 734.8 0 101 298 52 734.8 0 52 299 36 734.8 0 36 300 27 734.8 0 27 301 24 734.8 0 24 Finished in 13.48 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 327,818 Reads with adapters: 290,950 (88.8%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 290,948 (88.8%) Total basepairs processed: 92,130,327 bp Total written (filtered): 79,433,329 bp (86.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 290950 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 17 0.0 3 0 0 0 5 12 18 101 0.0 3 0 0 97 3 1 19 2597 0.0 3 0 2567 25 2 3 20 287204 0.0 4 283850 3238 109 4 3 21 712 0.0 4 126 580 5 0 1 22 52 0.0 4 5 4 39 0 4 23 184 0.0 4 68 3 4 106 3 24 13 0.0 4 2 1 0 1 9 25 1 0.0 4 1 26 2 0.0 4 1 0 1 27 2 0.0 4 0 2 29 1 0.0 4 1 30 1 0.0 4 1 32 1 0.0 4 1 38 1 0.0 4 1 41 9 0.0 4 0 0 0 0 9 42 1 0.0 4 1 96 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 2 110 1 0.0 4 0 0 0 0 1 111 26 0.0 4 0 0 0 26 127 21 0.0 4 0 0 0 0 21 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 10.02 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 290,948 Reads with adapters: 11,444 (3.9%) Reads written (passing filters): 290,948 (100.0%) Total basepairs processed: 79,433,329 bp Total written (filtered): 79,022,479 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11444 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 1.2% G: 88.3% T: 0.6% none/other: 9.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 4546.1 0 15 4 19 1136.5 0 19 5 6 284.1 0 6 6 3 71.0 0 3 8 1 4.4 0 1 9 6 1.1 0 0 6 10 2041 0.3 1 55 1986 11 8226 0.1 1 28 8198 12 6 0.0 1 0 6 13 4 0.0 1 0 4 14 3 0.0 1 0 3 19 1 0.0 1 1 23 7 0.0 1 5 2 82 1 0.0 1 1 105 1 0.0 1 0 1 139 1 0.0 1 1 151 7 0.0 1 0 7 152 2 0.0 1 0 2 209 2 0.0 1 1 1 223 1 0.0 1 1 224 7 0.0 1 6 1 258 1 0.0 1 1 259 3 0.0 1 3 260 22 0.0 1 22 261 10 0.0 1 10 263 2 0.0 1 2 267 1 0.0 1 1 271 5 0.0 1 5 272 1 0.0 1 1 273 1004 0.0 1 983 21 274 21 0.0 1 21 275 14 0.0 1 14 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.