This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.34 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 469,794 (15.6%) Reads that were too short: 338,142 (11.2%) Reads written (passing filters): 131,652 (4.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 31,142,291 bp (3.4%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 469794 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 636 734.8 0 636 7 62463 734.8 0 62463 8 159 734.8 0 159 9 3972 734.8 0 3972 10 73 734.8 0 73 11 71 734.8 0 71 12 60 734.8 0 60 13 60 734.8 0 60 14 67 734.8 0 67 15 61 734.8 0 61 16 58 734.8 0 58 17 62 734.8 0 62 18 60 734.8 0 60 19 56 734.8 0 56 20 59 734.8 0 59 21 68 734.8 0 68 22 60 734.8 0 60 23 51 734.8 0 51 24 76 734.8 0 76 25 65 734.8 0 65 26 54 734.8 0 54 27 56 734.8 0 56 28 87 734.8 0 87 29 81 734.8 0 81 30 149 734.8 0 149 31 611 734.8 0 611 32 59 734.8 0 59 33 75 734.8 0 75 34 80 734.8 0 80 35 80 734.8 0 80 36 106 734.8 0 106 37 107 734.8 0 107 38 98 734.8 0 98 39 50 734.8 0 50 40 55 734.8 0 55 41 41 734.8 0 41 42 42 734.8 0 42 43 57 734.8 0 57 44 61 734.8 0 61 45 56 734.8 0 56 46 60 734.8 0 60 47 44 734.8 0 44 48 40 734.8 0 40 49 37 734.8 0 37 50 33 734.8 0 33 51 43 734.8 0 43 52 44 734.8 0 44 53 48 734.8 0 48 54 49 734.8 0 49 55 58 734.8 0 58 56 2268 734.8 0 2268 57 339 734.8 0 339 58 46 734.8 0 46 59 48 734.8 0 48 60 67 734.8 0 67 61 50 734.8 0 50 62 37 734.8 0 37 63 49 734.8 0 49 64 50 734.8 0 50 65 73 734.8 0 73 66 86 734.8 0 86 67 57 734.8 0 57 68 68 734.8 0 68 69 100 734.8 0 100 70 115 734.8 0 115 71 109 734.8 0 109 72 161 734.8 0 161 73 241 734.8 0 241 74 242 734.8 0 242 75 602 734.8 0 602 76 464 734.8 0 464 77 729 734.8 0 729 78 823 734.8 0 823 79 308 734.8 0 308 80 546 734.8 0 546 81 551 734.8 0 551 82 409 734.8 0 409 83 207 734.8 0 207 84 311 734.8 0 311 85 277 734.8 0 277 86 232 734.8 0 232 87 226 734.8 0 226 88 426 734.8 0 426 89 306 734.8 0 306 90 470 734.8 0 470 91 253 734.8 0 253 92 355 734.8 0 355 93 438 734.8 0 438 94 412 734.8 0 412 95 341 734.8 0 341 96 869 734.8 0 869 97 850 734.8 0 850 98 1015 734.8 0 1015 99 964 734.8 0 964 100 552 734.8 0 552 101 385 734.8 0 385 102 211 734.8 0 211 103 149 734.8 0 149 104 124 734.8 0 124 105 291 734.8 0 291 106 999 734.8 0 999 107 704 734.8 0 704 108 659 734.8 0 659 109 439 734.8 0 439 110 669 734.8 0 669 111 627 734.8 0 627 112 601 734.8 0 601 113 483 734.8 0 483 114 468 734.8 0 468 115 298 734.8 0 298 116 208 734.8 0 208 117 204 734.8 0 204 118 242 734.8 0 242 119 694 734.8 0 694 120 643 734.8 0 643 121 445 734.8 0 445 122 283 734.8 0 283 123 641 734.8 0 641 124 509 734.8 0 509 125 355 734.8 0 355 126 346 734.8 0 346 127 320 734.8 0 320 128 515 734.8 0 515 129 463 734.8 0 463 130 347 734.8 0 347 131 326 734.8 0 326 132 655 734.8 0 655 133 377 734.8 0 377 134 442 734.8 0 442 135 328 734.8 0 328 136 285 734.8 0 285 137 255 734.8 0 255 138 371 734.8 0 371 139 1042 734.8 0 1042 140 872 734.8 0 872 141 505 734.8 0 505 142 241 734.8 0 241 143 242 734.8 0 242 144 241 734.8 0 241 145 269 734.8 0 269 146 395 734.8 0 395 147 278 734.8 0 278 148 305 734.8 0 305 149 277 734.8 0 277 150 252 734.8 0 252 151 176 734.8 0 176 152 212 734.8 0 212 153 224 734.8 0 224 154 275 734.8 0 275 155 327 734.8 0 327 156 300 734.8 0 300 157 311 734.8 0 311 158 335 734.8 0 335 159 2451 734.8 0 2451 160 4779 734.8 0 4779 161 2051 734.8 0 2051 162 229 734.8 0 229 163 258 734.8 0 258 164 246 734.8 0 246 165 326 734.8 0 326 166 248 734.8 0 248 167 276 734.8 0 276 168 300 734.8 0 300 169 186 734.8 0 186 170 149 734.8 0 149 171 147 734.8 0 147 172 202 734.8 0 202 173 292 734.8 0 292 174 375 734.8 0 375 175 224 734.8 0 224 176 254 734.8 0 254 177 194 734.8 0 194 178 220 734.8 0 220 179 221 734.8 0 221 180 313 734.8 0 313 181 247 734.8 0 247 182 184 734.8 0 184 183 301 734.8 0 301 184 363 734.8 0 363 185 371 734.8 0 371 186 237 734.8 0 237 187 225 734.8 0 225 188 246 734.8 0 246 189 272 734.8 0 272 190 254 734.8 0 254 191 250 734.8 0 250 192 316 734.8 0 316 193 310 734.8 0 310 194 342 734.8 0 342 195 472 734.8 0 472 196 396 734.8 0 396 197 218 734.8 0 218 198 202 734.8 0 202 199 213 734.8 0 213 200 448 734.8 0 448 201 564 734.8 0 564 202 359 734.8 0 359 203 447 734.8 0 447 204 463 734.8 0 463 205 403 734.8 0 403 206 595 734.8 0 595 207 978 734.8 0 978 208 755 734.8 0 755 209 702 734.8 0 702 210 530 734.8 0 530 211 504 734.8 0 504 212 750 734.8 0 750 213 580 734.8 0 580 214 351 734.8 0 351 215 269 734.8 0 269 216 205 734.8 0 205 217 212 734.8 0 212 218 487 734.8 0 487 219 384 734.8 0 384 220 458 734.8 0 458 221 585 734.8 0 585 222 404 734.8 0 404 223 518 734.8 0 518 224 510 734.8 0 510 225 341 734.8 0 341 226 179 734.8 0 179 227 318 734.8 0 318 228 549 734.8 0 549 229 370 734.8 0 370 230 436 734.8 0 436 231 509 734.8 0 509 232 4652 734.8 0 4652 233 2883 734.8 0 2883 234 1031 734.8 0 1031 235 315 734.8 0 315 236 328 734.8 0 328 237 544 734.8 0 544 238 867 734.8 0 867 239 302 734.8 0 302 240 799 734.8 0 799 241 728 734.8 0 728 242 700 734.8 0 700 243 884 734.8 0 884 244 476 734.8 0 476 245 376 734.8 0 376 246 193 734.8 0 193 247 293 734.8 0 293 248 550 734.8 0 550 249 1005 734.8 0 1005 250 1568 734.8 0 1568 251 1871 734.8 0 1871 252 2292 734.8 0 2292 253 5229 734.8 0 5229 254 10704 734.8 0 10704 255 131030 734.8 0 131030 256 112138 734.8 0 112138 257 24150 734.8 0 24150 258 544 734.8 0 544 259 356 734.8 0 356 260 582 734.8 0 582 261 1101 734.8 0 1101 262 2044 734.8 0 2044 263 1099 734.8 0 1099 264 282 734.8 0 282 265 239 734.8 0 239 266 261 734.8 0 261 267 185 734.8 0 185 268 160 734.8 0 160 269 320 734.8 0 320 270 302 734.8 0 302 271 383 734.8 0 383 272 382 734.8 0 382 273 247 734.8 0 247 274 140 734.8 0 140 275 232 734.8 0 232 276 292 734.8 0 292 277 225 734.8 0 225 278 160 734.8 0 160 279 227 734.8 0 227 280 225 734.8 0 225 281 225 734.8 0 225 282 498 734.8 0 498 283 633 734.8 0 633 284 415 734.8 0 415 285 127 734.8 0 127 286 100 734.8 0 100 287 159 734.8 0 159 288 239 734.8 0 239 289 332 734.8 0 332 290 337 734.8 0 337 291 232 734.8 0 232 292 208 734.8 0 208 293 209 734.8 0 209 294 199 734.8 0 199 295 328 734.8 0 328 296 498 734.8 0 498 297 463 734.8 0 463 298 869 734.8 0 869 299 952 734.8 0 952 300 458 734.8 0 458 301 614 734.8 0 614 Finished in 5.16 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 131,652 Reads with adapters: 4,072 (3.1%) Reads that were too short: 70 (0.1%) Reads written (passing filters): 4,002 (3.0%) Total basepairs processed: 31,142,291 bp Total written (filtered): 1,094,754 bp (3.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4072 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 66 0.0 3 0 65 1 17 3859 0.0 3 3746 99 9 5 18 22 0.0 3 0 13 1 8 19 5 0.0 3 0 1 2 2 20 10 0.0 3 0 0 0 10 24 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 115 4 0.0 3 0 0 0 4 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 199 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 10 0.0 3 0 0 0 10 282 20 0.0 3 0 0 0 20 283 10 0.0 3 0 0 0 10 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 4,002 Reads with adapters: 242 (6.0%) Reads written (passing filters): 4,002 (100.0%) Total basepairs processed: 1,094,754 bp Total written (filtered): 1,094,024 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 242 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.2% C: 6.6% G: 26.4% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 238 62.5 0 238 4 4 15.6 0 4