This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.24 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 49,691 (1.7%) Reads that were too short: 1,762 (0.1%) Reads written (passing filters): 47,929 (1.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 13,660,679 bp (1.5%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 49691 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 436 734.8 0 436 7 961 734.8 0 961 8 37861 734.8 0 37861 9 5034 734.8 0 5034 10 21 734.8 0 21 11 48 734.8 0 48 12 15 734.8 0 15 13 8 734.8 0 8 14 15 734.8 0 15 15 7 734.8 0 7 16 10 734.8 0 10 17 11 734.8 0 11 18 11 734.8 0 11 19 9 734.8 0 9 20 9 734.8 0 9 21 10 734.8 0 10 22 16 734.8 0 16 23 9 734.8 0 9 24 9 734.8 0 9 25 11 734.8 0 11 26 14 734.8 0 14 27 12 734.8 0 12 28 16 734.8 0 16 29 12 734.8 0 12 30 17 734.8 0 17 31 10 734.8 0 10 32 11 734.8 0 11 33 11 734.8 0 11 34 10 734.8 0 10 35 14 734.8 0 14 36 24 734.8 0 24 37 18 734.8 0 18 38 14 734.8 0 14 39 5 734.8 0 5 40 15 734.8 0 15 41 17 734.8 0 17 42 17 734.8 0 17 43 12 734.8 0 12 44 13 734.8 0 13 45 11 734.8 0 11 46 10 734.8 0 10 47 14 734.8 0 14 48 171 734.8 0 171 49 101 734.8 0 101 50 74 734.8 0 74 51 12 734.8 0 12 52 11 734.8 0 11 53 12 734.8 0 12 54 9 734.8 0 9 55 17 734.8 0 17 56 17 734.8 0 17 57 20 734.8 0 20 58 9 734.8 0 9 59 12 734.8 0 12 60 17 734.8 0 17 61 18 734.8 0 18 62 8 734.8 0 8 63 29 734.8 0 29 64 20 734.8 0 20 65 10 734.8 0 10 66 10 734.8 0 10 67 6 734.8 0 6 68 9 734.8 0 9 69 10 734.8 0 10 70 16 734.8 0 16 71 10 734.8 0 10 72 7 734.8 0 7 73 15 734.8 0 15 74 10 734.8 0 10 75 10 734.8 0 10 76 9 734.8 0 9 77 12 734.8 0 12 78 15 734.8 0 15 79 6 734.8 0 6 80 14 734.8 0 14 81 20 734.8 0 20 82 11 734.8 0 11 83 17 734.8 0 17 84 25 734.8 0 25 85 22 734.8 0 22 86 11 734.8 0 11 87 11 734.8 0 11 88 10 734.8 0 10 89 19 734.8 0 19 90 11 734.8 0 11 91 9 734.8 0 9 92 11 734.8 0 11 93 6 734.8 0 6 94 8 734.8 0 8 95 18 734.8 0 18 96 199 734.8 0 199 97 15 734.8 0 15 98 13 734.8 0 13 99 13 734.8 0 13 100 10 734.8 0 10 101 10 734.8 0 10 102 9 734.8 0 9 103 13 734.8 0 13 104 11 734.8 0 11 105 9 734.8 0 9 106 6 734.8 0 6 107 49 734.8 0 49 108 7 734.8 0 7 109 9 734.8 0 9 110 13 734.8 0 13 111 11 734.8 0 11 112 7 734.8 0 7 113 14 734.8 0 14 114 16 734.8 0 16 115 12 734.8 0 12 116 12 734.8 0 12 117 12 734.8 0 12 118 64 734.8 0 64 119 37 734.8 0 37 120 16 734.8 0 16 121 11 734.8 0 11 122 16 734.8 0 16 123 15 734.8 0 15 124 16 734.8 0 16 125 10 734.8 0 10 126 15 734.8 0 15 127 23 734.8 0 23 128 11 734.8 0 11 129 22 734.8 0 22 130 12 734.8 0 12 131 10 734.8 0 10 132 9 734.8 0 9 133 24 734.8 0 24 134 8 734.8 0 8 135 7 734.8 0 7 136 36 734.8 0 36 137 29 734.8 0 29 138 20 734.8 0 20 139 14 734.8 0 14 140 10 734.8 0 10 141 15 734.8 0 15 142 17 734.8 0 17 143 15 734.8 0 15 144 12 734.8 0 12 145 15 734.8 0 15 146 18 734.8 0 18 147 33 734.8 0 33 148 29 734.8 0 29 149 9 734.8 0 9 150 5 734.8 0 5 151 10 734.8 0 10 152 12 734.8 0 12 153 7 734.8 0 7 154 7 734.8 0 7 155 14 734.8 0 14 156 9 734.8 0 9 157 13 734.8 0 13 158 11 734.8 0 11 159 7 734.8 0 7 160 4 734.8 0 4 161 13 734.8 0 13 162 11 734.8 0 11 163 9 734.8 0 9 164 11 734.8 0 11 165 5 734.8 0 5 166 10 734.8 0 10 167 11 734.8 0 11 168 14 734.8 0 14 169 8 734.8 0 8 170 15 734.8 0 15 171 18 734.8 0 18 172 9 734.8 0 9 173 6 734.8 0 6 174 4 734.8 0 4 175 18 734.8 0 18 176 8 734.8 0 8 177 11 734.8 0 11 178 16 734.8 0 16 179 11 734.8 0 11 180 15 734.8 0 15 181 18 734.8 0 18 182 51 734.8 0 51 183 11 734.8 0 11 184 13 734.8 0 13 185 14 734.8 0 14 186 15 734.8 0 15 187 7 734.8 0 7 188 11 734.8 0 11 189 16 734.8 0 16 190 16 734.8 0 16 191 16 734.8 0 16 192 18 734.8 0 18 193 40 734.8 0 40 194 42 734.8 0 42 195 231 734.8 0 231 196 181 734.8 0 181 197 61 734.8 0 61 198 12 734.8 0 12 199 7 734.8 0 7 200 8 734.8 0 8 201 8 734.8 0 8 202 15 734.8 0 15 203 11 734.8 0 11 204 3 734.8 0 3 205 9 734.8 0 9 206 15 734.8 0 15 207 10 734.8 0 10 208 12 734.8 0 12 209 9 734.8 0 9 210 10 734.8 0 10 211 13 734.8 0 13 212 10 734.8 0 10 213 25 734.8 0 25 214 30 734.8 0 30 215 25 734.8 0 25 216 13 734.8 0 13 217 4 734.8 0 4 218 23 734.8 0 23 219 15 734.8 0 15 220 17 734.8 0 17 221 14 734.8 0 14 222 12 734.8 0 12 223 8 734.8 0 8 224 7 734.8 0 7 225 18 734.8 0 18 226 15 734.8 0 15 227 9 734.8 0 9 228 14 734.8 0 14 229 14 734.8 0 14 230 22 734.8 0 22 231 17 734.8 0 17 232 12 734.8 0 12 233 13 734.8 0 13 234 36 734.8 0 36 235 41 734.8 0 41 236 9 734.8 0 9 237 22 734.8 0 22 238 28 734.8 0 28 239 24 734.8 0 24 240 23 734.8 0 23 241 15 734.8 0 15 242 9 734.8 0 9 243 22 734.8 0 22 244 46 734.8 0 46 245 33 734.8 0 33 246 32 734.8 0 32 247 23 734.8 0 23 248 25 734.8 0 25 249 8 734.8 0 8 250 12 734.8 0 12 251 62 734.8 0 62 252 32 734.8 0 32 253 16 734.8 0 16 254 15 734.8 0 15 255 18 734.8 0 18 256 20 734.8 0 20 257 22 734.8 0 22 258 14 734.8 0 14 259 16 734.8 0 16 260 14 734.8 0 14 261 36 734.8 0 36 262 17 734.8 0 17 263 14 734.8 0 14 264 10 734.8 0 10 265 10 734.8 0 10 266 9 734.8 0 9 267 12 734.8 0 12 268 24 734.8 0 24 269 21 734.8 0 21 270 12 734.8 0 12 271 11 734.8 0 11 272 12 734.8 0 12 273 11 734.8 0 11 274 11 734.8 0 11 275 26 734.8 0 26 276 13 734.8 0 13 277 10 734.8 0 10 278 11 734.8 0 11 279 34 734.8 0 34 280 18 734.8 0 18 281 10 734.8 0 10 282 22 734.8 0 22 283 10 734.8 0 10 284 9 734.8 0 9 285 15 734.8 0 15 286 15 734.8 0 15 287 18 734.8 0 18 288 8 734.8 0 8 289 23 734.8 0 23 290 7 734.8 0 7 291 17 734.8 0 17 292 20 734.8 0 20 293 16 734.8 0 16 294 24 734.8 0 24 295 18 734.8 0 18 296 33 734.8 0 33 297 11 734.8 0 11 298 12 734.8 0 12 299 12 734.8 0 12 300 25 734.8 0 25 301 34 734.8 0 34 Finished in 2.56 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 47,929 Reads with adapters: 5,812 (12.1%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 5,810 (12.1%) Total basepairs processed: 13,660,679 bp Total written (filtered): 1,570,192 bp (11.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5812 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 102 0.0 3 0 98 1 0 3 20 4997 0.0 4 4934 62 1 21 21 0.0 4 3 17 0 0 1 22 571 0.0 4 564 5 2 23 7 0.0 4 1 3 0 2 1 24 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 86 3 0.0 4 0 0 0 0 3 105 1 0.0 4 0 0 0 0 1 106 27 0.0 4 0 0 1 23 3 107 1 0.0 4 0 0 0 0 1 108 7 0.0 4 0 0 0 0 7 109 2 0.0 4 0 0 0 2 110 1 0.0 4 0 0 0 0 1 111 9 0.0 4 0 0 0 8 1 119 1 0.0 4 0 1 126 1 0.0 4 0 0 0 0 1 127 53 0.0 4 1 0 0 0 52 153 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,810 Reads with adapters: 166 (2.9%) Reads written (passing filters): 5,810 (100.0%) Total basepairs processed: 1,570,192 bp Total written (filtered): 1,557,724 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 166 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 26.5% C: 3.0% G: 68.7% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 2 22.7 0 2 6 1 1.4 0 1 9 22 0.0 0 0 22 10 79 0.0 1 0 79 11 18 0.0 1 0 18 228 2 0.0 1 2 255 1 0.0 1 0 1 257 35 0.0 1 29 6 258 2 0.0 1 2 259 2 0.0 1 2 260 1 0.0 1 0 1 268 1 0.0 1 1