This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.63 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 67,752 (2.3%) Reads that were too short: 14,871 (0.5%) Reads written (passing filters): 52,881 (1.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 14,221,596 bp (1.6%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 67752 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1405 734.8 0 1405 7 1946 734.8 0 1946 8 35753 734.8 0 35753 9 67 734.8 0 67 10 42 734.8 0 42 11 24 734.8 0 24 12 24 734.8 0 24 13 24 734.8 0 24 14 29 734.8 0 29 15 25 734.8 0 25 16 27 734.8 0 27 17 37 734.8 0 37 18 23 734.8 0 23 19 22 734.8 0 22 20 36 734.8 0 36 21 26 734.8 0 26 22 31 734.8 0 31 23 31 734.8 0 31 24 20 734.8 0 20 25 40 734.8 0 40 26 33 734.8 0 33 27 25 734.8 0 25 28 28 734.8 0 28 29 36 734.8 0 36 30 27 734.8 0 27 31 23 734.8 0 23 32 31 734.8 0 31 33 37 734.8 0 37 34 20 734.8 0 20 35 33 734.8 0 33 36 42 734.8 0 42 37 27 734.8 0 27 38 22 734.8 0 22 39 37 734.8 0 37 40 33 734.8 0 33 41 24 734.8 0 24 42 44 734.8 0 44 43 43 734.8 0 43 44 19 734.8 0 19 45 24 734.8 0 24 46 50 734.8 0 50 47 39 734.8 0 39 48 41 734.8 0 41 49 34 734.8 0 34 50 30 734.8 0 30 51 22 734.8 0 22 52 45 734.8 0 45 53 48 734.8 0 48 54 38 734.8 0 38 55 44 734.8 0 44 56 41 734.8 0 41 57 44 734.8 0 44 58 31 734.8 0 31 59 40 734.8 0 40 60 83 734.8 0 83 61 65 734.8 0 65 62 2686 734.8 0 2686 63 163 734.8 0 163 64 113 734.8 0 113 65 32 734.8 0 32 66 124 734.8 0 124 67 47 734.8 0 47 68 42 734.8 0 42 69 23 734.8 0 23 70 46 734.8 0 46 71 37 734.8 0 37 72 33 734.8 0 33 73 424 734.8 0 424 74 381 734.8 0 381 75 51 734.8 0 51 76 29 734.8 0 29 77 333 734.8 0 333 78 227 734.8 0 227 79 68 734.8 0 68 80 35 734.8 0 35 81 27 734.8 0 27 82 31 734.8 0 31 83 26 734.8 0 26 84 23 734.8 0 23 85 32 734.8 0 32 86 29 734.8 0 29 87 23 734.8 0 23 88 39 734.8 0 39 89 21 734.8 0 21 90 19 734.8 0 19 91 38 734.8 0 38 92 25 734.8 0 25 93 27 734.8 0 27 94 30 734.8 0 30 95 39 734.8 0 39 96 34 734.8 0 34 97 43 734.8 0 43 98 34 734.8 0 34 99 40 734.8 0 40 100 39 734.8 0 39 101 41 734.8 0 41 102 24 734.8 0 24 103 27 734.8 0 27 104 38 734.8 0 38 105 37 734.8 0 37 106 96 734.8 0 96 107 206 734.8 0 206 108 55 734.8 0 55 109 34 734.8 0 34 110 40 734.8 0 40 111 41 734.8 0 41 112 46 734.8 0 46 113 41 734.8 0 41 114 45 734.8 0 45 115 57 734.8 0 57 116 31 734.8 0 31 117 45 734.8 0 45 118 34 734.8 0 34 119 39 734.8 0 39 120 38 734.8 0 38 121 43 734.8 0 43 122 27 734.8 0 27 123 30 734.8 0 30 124 51 734.8 0 51 125 50 734.8 0 50 126 50 734.8 0 50 127 459 734.8 0 459 128 132 734.8 0 132 129 149 734.8 0 149 130 74 734.8 0 74 131 55 734.8 0 55 132 50 734.8 0 50 133 48 734.8 0 48 134 166 734.8 0 166 135 54 734.8 0 54 136 75 734.8 0 75 137 38 734.8 0 38 138 38 734.8 0 38 139 74 734.8 0 74 140 38 734.8 0 38 141 32 734.8 0 32 142 39 734.8 0 39 143 41 734.8 0 41 144 73 734.8 0 73 145 61 734.8 0 61 146 683 734.8 0 683 147 561 734.8 0 561 148 85 734.8 0 85 149 42 734.8 0 42 150 22 734.8 0 22 151 29 734.8 0 29 152 24 734.8 0 24 153 20 734.8 0 20 154 33 734.8 0 33 155 56 734.8 0 56 156 139 734.8 0 139 157 32 734.8 0 32 158 24 734.8 0 24 159 42 734.8 0 42 160 53 734.8 0 53 161 29 734.8 0 29 162 28 734.8 0 28 163 23 734.8 0 23 164 70 734.8 0 70 165 91 734.8 0 91 166 65 734.8 0 65 167 49 734.8 0 49 168 62 734.8 0 62 169 39 734.8 0 39 170 31 734.8 0 31 171 34 734.8 0 34 172 30 734.8 0 30 173 21 734.8 0 21 174 15 734.8 0 15 175 29 734.8 0 29 176 15 734.8 0 15 177 21 734.8 0 21 178 22 734.8 0 22 179 28 734.8 0 28 180 16 734.8 0 16 181 31 734.8 0 31 182 36 734.8 0 36 183 26 734.8 0 26 184 129 734.8 0 129 185 118 734.8 0 118 186 61 734.8 0 61 187 28 734.8 0 28 188 28 734.8 0 28 189 21 734.8 0 21 190 21 734.8 0 21 191 31 734.8 0 31 192 27 734.8 0 27 193 22 734.8 0 22 194 22 734.8 0 22 195 98 734.8 0 98 196 50 734.8 0 50 197 71 734.8 0 71 198 26 734.8 0 26 199 22 734.8 0 22 200 22 734.8 0 22 201 18 734.8 0 18 202 27 734.8 0 27 203 24 734.8 0 24 204 96 734.8 0 96 205 34 734.8 0 34 206 43 734.8 0 43 207 21 734.8 0 21 208 34 734.8 0 34 209 26 734.8 0 26 210 29 734.8 0 29 211 21 734.8 0 21 212 23 734.8 0 23 213 33 734.8 0 33 214 29 734.8 0 29 215 50 734.8 0 50 216 60 734.8 0 60 217 52 734.8 0 52 218 32 734.8 0 32 219 41 734.8 0 41 220 37 734.8 0 37 221 33 734.8 0 33 222 36 734.8 0 36 223 40 734.8 0 40 224 30 734.8 0 30 225 41 734.8 0 41 226 41 734.8 0 41 227 39 734.8 0 39 228 29 734.8 0 29 229 37 734.8 0 37 230 37 734.8 0 37 231 54 734.8 0 54 232 61 734.8 0 61 233 84 734.8 0 84 234 109 734.8 0 109 235 174 734.8 0 174 236 1903 734.8 0 1903 237 7594 734.8 0 7594 238 729 734.8 0 729 239 30 734.8 0 30 240 31 734.8 0 31 241 28 734.8 0 28 242 27 734.8 0 27 243 17 734.8 0 17 244 20 734.8 0 20 245 36 734.8 0 36 246 57 734.8 0 57 247 47 734.8 0 47 248 29 734.8 0 29 249 22 734.8 0 22 250 14 734.8 0 14 251 20 734.8 0 20 252 42 734.8 0 42 253 33 734.8 0 33 254 35 734.8 0 35 255 32 734.8 0 32 256 26 734.8 0 26 257 31 734.8 0 31 258 22 734.8 0 22 259 32 734.8 0 32 260 19 734.8 0 19 261 23 734.8 0 23 262 21 734.8 0 21 263 26 734.8 0 26 264 25 734.8 0 25 265 31 734.8 0 31 266 29 734.8 0 29 267 24 734.8 0 24 268 12 734.8 0 12 269 21 734.8 0 21 270 26 734.8 0 26 271 20 734.8 0 20 272 34 734.8 0 34 273 34 734.8 0 34 274 20 734.8 0 20 275 30 734.8 0 30 276 52 734.8 0 52 277 103 734.8 0 103 278 161 734.8 0 161 279 69 734.8 0 69 280 155 734.8 0 155 281 475 734.8 0 475 282 335 734.8 0 335 283 54 734.8 0 54 284 78 734.8 0 78 285 85 734.8 0 85 286 30 734.8 0 30 287 16 734.8 0 16 288 28 734.8 0 28 289 22 734.8 0 22 290 19 734.8 0 19 291 18 734.8 0 18 292 30 734.8 0 30 293 32 734.8 0 32 294 22 734.8 0 22 295 19 734.8 0 19 296 36 734.8 0 36 297 63 734.8 0 63 298 45 734.8 0 45 299 29 734.8 0 29 300 51 734.8 0 51 301 35 734.8 0 35 Finished in 2.45 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 52,881 Reads with adapters: 37,528 (71.0%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 37,528 (71.0%) Total basepairs processed: 14,221,596 bp Total written (filtered): 10,240,975 bp (72.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37528 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 0 5 18 25 0.0 3 0 0 24 1 19 536 0.0 3 0 527 6 1 2 20 35948 0.0 4 35488 449 10 1 21 108 0.0 4 20 88 22 828 0.0 4 807 8 10 0 3 23 15 0.0 4 2 1 0 10 2 24 1 0.0 4 0 1 56 1 0.0 4 0 0 0 0 1 57 18 0.0 4 0 0 0 0 18 61 5 0.0 4 0 0 0 0 5 83 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 1 1 109 6 0.0 4 0 0 0 6 111 26 0.0 4 0 0 0 23 3 113 2 0.0 4 0 0 0 2 123 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.29 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 37,528 Reads with adapters: 1,286 (3.4%) Reads written (passing filters): 37,528 (100.0%) Total basepairs processed: 10,240,975 bp Total written (filtered): 10,210,883 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1286 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.8% C: 1.5% G: 92.8% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 586.4 0 2 4 6 146.6 0 6 6 1 9.2 0 1 9 3 0.1 0 0 3 10 385 0.0 1 14 371 11 819 0.0 1 1 818 12 2 0.0 1 0 2 14 1 0.0 1 0 1 49 1 0.0 1 1 193 1 0.0 1 0 1 259 53 0.0 1 52 1 260 2 0.0 1 2 261 2 0.0 1 2 262 4 0.0 1 4 265 1 0.0 1 1 268 1 0.0 1 1 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.