This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.29 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 222,612 (7.4%) Reads that were too short: 112,688 (3.7%) Reads written (passing filters): 109,924 (3.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 21,692,410 bp (2.4%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 222612 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 460 734.8 0 460 7 29432 734.8 0 29432 8 71 734.8 0 71 9 2098 734.8 0 2098 10 15 734.8 0 15 11 8 734.8 0 8 12 24 734.8 0 24 13 23 734.8 0 23 14 7 734.8 0 7 15 19 734.8 0 19 16 19 734.8 0 19 17 19 734.8 0 19 18 6 734.8 0 6 19 8 734.8 0 8 20 10 734.8 0 10 21 16 734.8 0 16 22 15 734.8 0 15 23 10 734.8 0 10 24 11 734.8 0 11 25 11 734.8 0 11 26 8 734.8 0 8 27 6 734.8 0 6 28 13 734.8 0 13 29 7 734.8 0 7 30 13 734.8 0 13 31 11 734.8 0 11 32 10 734.8 0 10 33 8 734.8 0 8 34 15 734.8 0 15 35 16 734.8 0 16 36 5 734.8 0 5 37 9 734.8 0 9 38 11 734.8 0 11 39 14 734.8 0 14 40 133 734.8 0 133 41 16 734.8 0 16 42 19 734.8 0 19 43 12 734.8 0 12 44 12 734.8 0 12 45 11 734.8 0 11 46 15 734.8 0 15 47 7 734.8 0 7 48 10 734.8 0 10 49 5 734.8 0 5 50 13 734.8 0 13 51 6 734.8 0 6 52 3 734.8 0 3 53 5 734.8 0 5 54 10 734.8 0 10 55 19 734.8 0 19 56 12 734.8 0 12 57 6 734.8 0 6 58 9 734.8 0 9 59 13 734.8 0 13 60 12 734.8 0 12 61 18 734.8 0 18 62 17 734.8 0 17 63 21 734.8 0 21 64 29 734.8 0 29 65 415 734.8 0 415 66 283 734.8 0 283 67 232 734.8 0 232 68 4 734.8 0 4 69 6 734.8 0 6 70 15 734.8 0 15 71 18 734.8 0 18 72 23 734.8 0 23 73 12 734.8 0 12 74 13 734.8 0 13 75 6 734.8 0 6 76 12 734.8 0 12 77 8 734.8 0 8 78 40 734.8 0 40 79 71 734.8 0 71 80 88 734.8 0 88 81 579 734.8 0 579 82 494 734.8 0 494 83 437 734.8 0 437 84 259 734.8 0 259 85 329 734.8 0 329 86 739 734.8 0 739 87 639 734.8 0 639 88 585 734.8 0 585 89 428 734.8 0 428 90 162 734.8 0 162 91 278 734.8 0 278 92 230 734.8 0 230 93 408 734.8 0 408 94 434 734.8 0 434 95 206 734.8 0 206 96 269 734.8 0 269 97 618 734.8 0 618 98 589 734.8 0 589 99 462 734.8 0 462 100 270 734.8 0 270 101 290 734.8 0 290 102 761 734.8 0 761 103 557 734.8 0 557 104 349 734.8 0 349 105 459 734.8 0 459 106 398 734.8 0 398 107 633 734.8 0 633 108 559 734.8 0 559 109 582 734.8 0 582 110 754 734.8 0 754 111 579 734.8 0 579 112 462 734.8 0 462 113 643 734.8 0 643 114 540 734.8 0 540 115 441 734.8 0 441 116 346 734.8 0 346 117 353 734.8 0 353 118 775 734.8 0 775 119 794 734.8 0 794 120 497 734.8 0 497 121 291 734.8 0 291 122 527 734.8 0 527 123 619 734.8 0 619 124 646 734.8 0 646 125 761 734.8 0 761 126 837 734.8 0 837 127 731 734.8 0 731 128 545 734.8 0 545 129 457 734.8 0 457 130 431 734.8 0 431 131 423 734.8 0 423 132 525 734.8 0 525 133 1096 734.8 0 1096 134 910 734.8 0 910 135 543 734.8 0 543 136 489 734.8 0 489 137 595 734.8 0 595 138 934 734.8 0 934 139 1218 734.8 0 1218 140 1009 734.8 0 1009 141 539 734.8 0 539 142 343 734.8 0 343 143 553 734.8 0 553 144 1038 734.8 0 1038 145 873 734.8 0 873 146 917 734.8 0 917 147 865 734.8 0 865 148 459 734.8 0 459 149 762 734.8 0 762 150 744 734.8 0 744 151 1112 734.8 0 1112 152 1110 734.8 0 1110 153 750 734.8 0 750 154 875 734.8 0 875 155 610 734.8 0 610 156 849 734.8 0 849 157 1297 734.8 0 1297 158 748 734.8 0 748 159 923 734.8 0 923 160 683 734.8 0 683 161 704 734.8 0 704 162 539 734.8 0 539 163 590 734.8 0 590 164 1075 734.8 0 1075 165 833 734.8 0 833 166 664 734.8 0 664 167 517 734.8 0 517 168 489 734.8 0 489 169 443 734.8 0 443 170 479 734.8 0 479 171 696 734.8 0 696 172 1003 734.8 0 1003 173 769 734.8 0 769 174 491 734.8 0 491 175 385 734.8 0 385 176 410 734.8 0 410 177 596 734.8 0 596 178 492 734.8 0 492 179 851 734.8 0 851 180 946 734.8 0 946 181 964 734.8 0 964 182 560 734.8 0 560 183 435 734.8 0 435 184 664 734.8 0 664 185 879 734.8 0 879 186 623 734.8 0 623 187 547 734.8 0 547 188 631 734.8 0 631 189 833 734.8 0 833 190 925 734.8 0 925 191 680 734.8 0 680 192 563 734.8 0 563 193 518 734.8 0 518 194 598 734.8 0 598 195 476 734.8 0 476 196 635 734.8 0 635 197 646 734.8 0 646 198 382 734.8 0 382 199 948 734.8 0 948 200 701 734.8 0 701 201 813 734.8 0 813 202 814 734.8 0 814 203 473 734.8 0 473 204 535 734.8 0 535 205 783 734.8 0 783 206 936 734.8 0 936 207 971 734.8 0 971 208 869 734.8 0 869 209 886 734.8 0 886 210 1203 734.8 0 1203 211 5177 734.8 0 5177 212 21077 734.8 0 21077 213 3888 734.8 0 3888 214 1731 734.8 0 1731 215 620 734.8 0 620 216 603 734.8 0 603 217 557 734.8 0 557 218 720 734.8 0 720 219 842 734.8 0 842 220 1100 734.8 0 1100 221 683 734.8 0 683 222 598 734.8 0 598 223 710 734.8 0 710 224 895 734.8 0 895 225 850 734.8 0 850 226 740 734.8 0 740 227 560 734.8 0 560 228 520 734.8 0 520 229 720 734.8 0 720 230 622 734.8 0 622 231 641 734.8 0 641 232 641 734.8 0 641 233 733 734.8 0 733 234 819 734.8 0 819 235 688 734.8 0 688 236 674 734.8 0 674 237 605 734.8 0 605 238 869 734.8 0 869 239 806 734.8 0 806 240 903 734.8 0 903 241 940 734.8 0 940 242 887 734.8 0 887 243 764 734.8 0 764 244 618 734.8 0 618 245 841 734.8 0 841 246 646 734.8 0 646 247 554 734.8 0 554 248 380 734.8 0 380 249 759 734.8 0 759 250 841 734.8 0 841 251 1043 734.8 0 1043 252 911 734.8 0 911 253 464 734.8 0 464 254 625 734.8 0 625 255 686 734.8 0 686 256 690 734.8 0 690 257 738 734.8 0 738 258 666 734.8 0 666 259 779 734.8 0 779 260 810 734.8 0 810 261 839 734.8 0 839 262 782 734.8 0 782 263 566 734.8 0 566 264 587 734.8 0 587 265 684 734.8 0 684 266 621 734.8 0 621 267 809 734.8 0 809 268 637 734.8 0 637 269 728 734.8 0 728 270 1430 734.8 0 1430 271 1812 734.8 0 1812 272 1008 734.8 0 1008 273 733 734.8 0 733 274 1007 734.8 0 1007 275 765 734.8 0 765 276 778 734.8 0 778 277 991 734.8 0 991 278 1031 734.8 0 1031 279 734 734.8 0 734 280 789 734.8 0 789 281 572 734.8 0 572 282 813 734.8 0 813 283 973 734.8 0 973 284 602 734.8 0 602 285 682 734.8 0 682 286 756 734.8 0 756 287 718 734.8 0 718 288 822 734.8 0 822 289 887 734.8 0 887 290 814 734.8 0 814 291 674 734.8 0 674 292 931 734.8 0 931 293 3639 734.8 0 3639 294 3139 734.8 0 3139 295 1183 734.8 0 1183 296 1051 734.8 0 1051 297 859 734.8 0 859 298 872 734.8 0 872 299 970 734.8 0 970 300 1002 734.8 0 1002 301 694 734.8 0 694 Finished in 4.40 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 109,924 Reads with adapters: 30,845 (28.1%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 30,818 (28.0%) Total basepairs processed: 21,692,410 bp Total written (filtered): 8,351,541 bp (38.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30845 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 9 0.0 3 0 0 7 2 19 257 0.0 3 0 251 5 1 20 29458 0.0 4 29036 409 11 0 2 21 71 0.0 4 10 60 1 22 4 0.0 4 1 0 3 23 17 0.0 4 1 0 0 16 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 33 2 0.0 4 0 0 0 1 1 34 2 0.0 4 1 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 52 3 0.0 4 0 0 0 1 2 53 900 0.0 4 0 0 2 822 76 54 2 0.0 4 0 0 0 0 2 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 1 60 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 1 71 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 109 20 0.0 4 0 0 0 20 111 21 0.0 4 0 0 0 20 1 112 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 37 0.0 4 0 0 0 0 37 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.04 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 30,818 Reads with adapters: 1,203 (3.9%) Reads written (passing filters): 30,818 (100.0%) Total basepairs processed: 8,351,541 bp Total written (filtered): 8,311,043 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1203 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 0.8% G: 88.4% T: 5.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 481.5 0 3 4 1 120.4 0 1 10 2 0.0 1 0 2 11 1081 0.0 1 3 1078 12 9 0.0 1 0 9 178 1 0.0 1 1 260 1 0.0 1 1 261 41 0.0 1 40 1 262 2 0.0 1 2 271 61 0.0 1 60 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.