This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.33 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 391,560 (13.0%) Reads that were too short: 145,249 (4.8%) Reads written (passing filters): 246,311 (8.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 47,612,893 bp (5.3%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 391560 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 629 734.8 0 629 7 45857 734.8 0 45857 8 122 734.8 0 122 9 4885 734.8 0 4885 10 40 734.8 0 40 11 31 734.8 0 31 12 20 734.8 0 20 13 19 734.8 0 19 14 9 734.8 0 9 15 16 734.8 0 16 16 10 734.8 0 10 17 16 734.8 0 16 18 12 734.8 0 12 19 16 734.8 0 16 20 9 734.8 0 9 21 17 734.8 0 17 22 20 734.8 0 20 23 24 734.8 0 24 24 29 734.8 0 29 25 11 734.8 0 11 26 18 734.8 0 18 27 18 734.8 0 18 28 20 734.8 0 20 29 25 734.8 0 25 30 22 734.8 0 22 31 14 734.8 0 14 32 10 734.8 0 10 33 15 734.8 0 15 34 10 734.8 0 10 35 21 734.8 0 21 36 27 734.8 0 27 37 21 734.8 0 21 38 19 734.8 0 19 39 21 734.8 0 21 40 245 734.8 0 245 41 22 734.8 0 22 42 16 734.8 0 16 43 16 734.8 0 16 44 12 734.8 0 12 45 14 734.8 0 14 46 10 734.8 0 10 47 17 734.8 0 17 48 19 734.8 0 19 49 21 734.8 0 21 50 20 734.8 0 20 51 23 734.8 0 23 52 19 734.8 0 19 53 33 734.8 0 33 54 131 734.8 0 131 55 33 734.8 0 33 56 33 734.8 0 33 57 18 734.8 0 18 58 56 734.8 0 56 59 57 734.8 0 57 60 27 734.8 0 27 61 25 734.8 0 25 62 37 734.8 0 37 63 277 734.8 0 277 64 60 734.8 0 60 65 80 734.8 0 80 66 30 734.8 0 30 67 27 734.8 0 27 68 34 734.8 0 34 69 304 734.8 0 304 70 112 734.8 0 112 71 696 734.8 0 696 72 1306 734.8 0 1306 73 2163 734.8 0 2163 74 2142 734.8 0 2142 75 788 734.8 0 788 76 957 734.8 0 957 77 1302 734.8 0 1302 78 1469 734.8 0 1469 79 2099 734.8 0 2099 80 1694 734.8 0 1694 81 1284 734.8 0 1284 82 1944 734.8 0 1944 83 2423 734.8 0 2423 84 1565 734.8 0 1565 85 2397 734.8 0 2397 86 3181 734.8 0 3181 87 2686 734.8 0 2686 88 1704 734.8 0 1704 89 2403 734.8 0 2403 90 744 734.8 0 744 91 1132 734.8 0 1132 92 2577 734.8 0 2577 93 1191 734.8 0 1191 94 1362 734.8 0 1362 95 1263 734.8 0 1263 96 724 734.8 0 724 97 435 734.8 0 435 98 613 734.8 0 613 99 723 734.8 0 723 100 1395 734.8 0 1395 101 1500 734.8 0 1500 102 1357 734.8 0 1357 103 1620 734.8 0 1620 104 1244 734.8 0 1244 105 869 734.8 0 869 106 477 734.8 0 477 107 524 734.8 0 524 108 1245 734.8 0 1245 109 1465 734.8 0 1465 110 1866 734.8 0 1866 111 1601 734.8 0 1601 112 1573 734.8 0 1573 113 1254 734.8 0 1254 114 1191 734.8 0 1191 115 1101 734.8 0 1101 116 1758 734.8 0 1758 117 1818 734.8 0 1818 118 2011 734.8 0 2011 119 1894 734.8 0 1894 120 1990 734.8 0 1990 121 1912 734.8 0 1912 122 1744 734.8 0 1744 123 1587 734.8 0 1587 124 1469 734.8 0 1469 125 1576 734.8 0 1576 126 1282 734.8 0 1282 127 1100 734.8 0 1100 128 1139 734.8 0 1139 129 1392 734.8 0 1392 130 1227 734.8 0 1227 131 1753 734.8 0 1753 132 1481 734.8 0 1481 133 1135 734.8 0 1135 134 1168 734.8 0 1168 135 1158 734.8 0 1158 136 1169 734.8 0 1169 137 1256 734.8 0 1256 138 1500 734.8 0 1500 139 1387 734.8 0 1387 140 1178 734.8 0 1178 141 1309 734.8 0 1309 142 1748 734.8 0 1748 143 1468 734.8 0 1468 144 1302 734.8 0 1302 145 1626 734.8 0 1626 146 1617 734.8 0 1617 147 1479 734.8 0 1479 148 1556 734.8 0 1556 149 1207 734.8 0 1207 150 1416 734.8 0 1416 151 1329 734.8 0 1329 152 1815 734.8 0 1815 153 1645 734.8 0 1645 154 1173 734.8 0 1173 155 1279 734.8 0 1279 156 1125 734.8 0 1125 157 1212 734.8 0 1212 158 1351 734.8 0 1351 159 1165 734.8 0 1165 160 1354 734.8 0 1354 161 1111 734.8 0 1111 162 1297 734.8 0 1297 163 1058 734.8 0 1058 164 952 734.8 0 952 165 929 734.8 0 929 166 1793 734.8 0 1793 167 4265 734.8 0 4265 168 1629 734.8 0 1629 169 1294 734.8 0 1294 170 3292 734.8 0 3292 171 3302 734.8 0 3302 172 1772 734.8 0 1772 173 1201 734.8 0 1201 174 1027 734.8 0 1027 175 1406 734.8 0 1406 176 1119 734.8 0 1119 177 1322 734.8 0 1322 178 1318 734.8 0 1318 179 1187 734.8 0 1187 180 1418 734.8 0 1418 181 2085 734.8 0 2085 182 2259 734.8 0 2259 183 2052 734.8 0 2052 184 1414 734.8 0 1414 185 1264 734.8 0 1264 186 1107 734.8 0 1107 187 1312 734.8 0 1312 188 1358 734.8 0 1358 189 1165 734.8 0 1165 190 1258 734.8 0 1258 191 1243 734.8 0 1243 192 1074 734.8 0 1074 193 1193 734.8 0 1193 194 875 734.8 0 875 195 1162 734.8 0 1162 196 973 734.8 0 973 197 1578 734.8 0 1578 198 1941 734.8 0 1941 199 1468 734.8 0 1468 200 1529 734.8 0 1529 201 919 734.8 0 919 202 1010 734.8 0 1010 203 1300 734.8 0 1300 204 1325 734.8 0 1325 205 1271 734.8 0 1271 206 1192 734.8 0 1192 207 997 734.8 0 997 208 867 734.8 0 867 209 881 734.8 0 881 210 936 734.8 0 936 211 1084 734.8 0 1084 212 1089 734.8 0 1089 213 1838 734.8 0 1838 214 1963 734.8 0 1963 215 1279 734.8 0 1279 216 950 734.8 0 950 217 985 734.8 0 985 218 1365 734.8 0 1365 219 1536 734.8 0 1536 220 1002 734.8 0 1002 221 885 734.8 0 885 222 908 734.8 0 908 223 1158 734.8 0 1158 224 1040 734.8 0 1040 225 1006 734.8 0 1006 226 979 734.8 0 979 227 1000 734.8 0 1000 228 1107 734.8 0 1107 229 1195 734.8 0 1195 230 888 734.8 0 888 231 891 734.8 0 891 232 967 734.8 0 967 233 1070 734.8 0 1070 234 1020 734.8 0 1020 235 999 734.8 0 999 236 706 734.8 0 706 237 1040 734.8 0 1040 238 1239 734.8 0 1239 239 1576 734.8 0 1576 240 1244 734.8 0 1244 241 1135 734.8 0 1135 242 1242 734.8 0 1242 243 1294 734.8 0 1294 244 1021 734.8 0 1021 245 935 734.8 0 935 246 1271 734.8 0 1271 247 1033 734.8 0 1033 248 1229 734.8 0 1229 249 1767 734.8 0 1767 250 1256 734.8 0 1256 251 1157 734.8 0 1157 252 1565 734.8 0 1565 253 1163 734.8 0 1163 254 875 734.8 0 875 255 763 734.8 0 763 256 818 734.8 0 818 257 783 734.8 0 783 258 748 734.8 0 748 259 792 734.8 0 792 260 1223 734.8 0 1223 261 1531 734.8 0 1531 262 2204 734.8 0 2204 263 2253 734.8 0 2253 264 1445 734.8 0 1445 265 1029 734.8 0 1029 266 1048 734.8 0 1048 267 3053 734.8 0 3053 268 8154 734.8 0 8154 269 3627 734.8 0 3627 270 1485 734.8 0 1485 271 969 734.8 0 969 272 1376 734.8 0 1376 273 1230 734.8 0 1230 274 931 734.8 0 931 275 903 734.8 0 903 276 875 734.8 0 875 277 1209 734.8 0 1209 278 1813 734.8 0 1813 279 1648 734.8 0 1648 280 5987 734.8 0 5987 281 4755 734.8 0 4755 282 2163 734.8 0 2163 283 1070 734.8 0 1070 284 1144 734.8 0 1144 285 936 734.8 0 936 286 1176 734.8 0 1176 287 1321 734.8 0 1321 288 942 734.8 0 942 289 1128 734.8 0 1128 290 1536 734.8 0 1536 291 1460 734.8 0 1460 292 1621 734.8 0 1621 293 1264 734.8 0 1264 294 1073 734.8 0 1073 295 1028 734.8 0 1028 296 1290 734.8 0 1290 297 1491 734.8 0 1491 298 1835 734.8 0 1835 299 4061 734.8 0 4061 300 3304 734.8 0 3304 301 2023 734.8 0 2023 Finished in 8.43 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 246,311 Reads with adapters: 5,328 (2.2%) Reads that were too short: 103 (0.0%) Reads written (passing filters): 5,225 (2.1%) Total basepairs processed: 47,612,893 bp Total written (filtered): 1,392,993 bp (2.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5328 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 54 0.0 3 0 52 2 17 4864 0.0 3 4740 108 13 3 18 10 0.0 3 1 4 3 2 19 8 0.0 3 0 1 2 5 20 11 0.0 3 0 0 0 11 21 65 0.0 3 0 0 1 64 23 2 0.0 3 0 0 1 1 24 47 0.0 3 0 0 1 46 25 2 0.0 3 0 0 0 2 26 2 0.0 3 0 0 0 2 27 4 0.0 3 1 0 0 3 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 4 0.0 3 0 0 0 4 39 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 43 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 45 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 52 5 0.0 3 0 0 0 5 53 2 0.0 3 0 0 0 2 57 2 0.0 3 0 0 0 2 64 3 0.0 3 0 0 0 3 66 77 0.0 3 0 0 0 77 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 70 3 0.0 3 0 0 0 3 71 4 0.0 3 0 0 0 4 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 1 1 89 4 0.0 3 0 0 0 4 91 2 0.0 3 0 0 1 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 122 5 0.0 3 0 0 0 5 123 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 139 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 200 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 210 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 0 5 282 11 0.0 3 0 0 0 11 283 6 0.0 3 0 0 0 6 289 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 5,225 Reads with adapters: 138 (2.6%) Reads written (passing filters): 5,225 (100.0%) Total basepairs processed: 1,392,993 bp Total written (filtered): 1,392,575 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 138 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.6% C: 12.3% G: 31.9% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 134 81.6 0 134 4 4 20.4 0 4