This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.59 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 193,816 (6.4%) Reads that were too short: 119,594 (4.0%) Reads written (passing filters): 74,222 (2.5%) Total basepairs processed: 905,907,359 bp Total written (filtered): 18,231,050 bp (2.0%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 193816 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 631 734.8 0 631 7 415 734.8 0 415 8 39739 734.8 0 39739 9 5371 734.8 0 5371 10 52 734.8 0 52 11 41 734.8 0 41 12 39 734.8 0 39 13 64 734.8 0 64 14 60 734.8 0 60 15 32 734.8 0 32 16 46 734.8 0 46 17 41 734.8 0 41 18 260 734.8 0 260 19 51 734.8 0 51 20 43 734.8 0 43 21 65 734.8 0 65 22 30 734.8 0 30 23 25 734.8 0 25 24 46 734.8 0 46 25 35 734.8 0 35 26 34 734.8 0 34 27 25 734.8 0 25 28 32 734.8 0 32 29 33 734.8 0 33 30 44 734.8 0 44 31 71 734.8 0 71 32 47 734.8 0 47 33 45 734.8 0 45 34 31 734.8 0 31 35 34 734.8 0 34 36 23 734.8 0 23 37 45 734.8 0 45 38 77 734.8 0 77 39 32 734.8 0 32 40 45 734.8 0 45 41 143 734.8 0 143 42 30 734.8 0 30 43 100 734.8 0 100 44 121 734.8 0 121 45 77 734.8 0 77 46 1172 734.8 0 1172 47 208 734.8 0 208 48 88 734.8 0 88 49 48 734.8 0 48 50 50 734.8 0 50 51 50 734.8 0 50 52 102 734.8 0 102 53 231 734.8 0 231 54 242 734.8 0 242 55 51 734.8 0 51 56 58 734.8 0 58 57 443 734.8 0 443 58 46 734.8 0 46 59 42 734.8 0 42 60 37 734.8 0 37 61 26 734.8 0 26 62 43 734.8 0 43 63 19 734.8 0 19 64 46 734.8 0 46 65 44 734.8 0 44 66 65 734.8 0 65 67 31 734.8 0 31 68 30 734.8 0 30 69 32 734.8 0 32 70 21 734.8 0 21 71 31 734.8 0 31 72 34 734.8 0 34 73 58 734.8 0 58 74 40 734.8 0 40 75 51 734.8 0 51 76 46 734.8 0 46 77 64 734.8 0 64 78 63 734.8 0 63 79 33 734.8 0 33 80 26 734.8 0 26 81 32 734.8 0 32 82 70 734.8 0 70 83 63 734.8 0 63 84 84 734.8 0 84 85 92 734.8 0 92 86 91 734.8 0 91 87 56 734.8 0 56 88 63 734.8 0 63 89 91 734.8 0 91 90 58 734.8 0 58 91 61 734.8 0 61 92 71 734.8 0 71 93 71 734.8 0 71 94 144 734.8 0 144 95 91 734.8 0 91 96 90 734.8 0 90 97 115 734.8 0 115 98 122 734.8 0 122 99 148 734.8 0 148 100 67 734.8 0 67 101 46 734.8 0 46 102 65 734.8 0 65 103 61 734.8 0 61 104 46 734.8 0 46 105 53 734.8 0 53 106 75 734.8 0 75 107 120 734.8 0 120 108 96 734.8 0 96 109 47 734.8 0 47 110 86 734.8 0 86 111 77 734.8 0 77 112 90 734.8 0 90 113 70 734.8 0 70 114 103 734.8 0 103 115 138 734.8 0 138 116 140 734.8 0 140 117 120 734.8 0 120 118 125 734.8 0 125 119 171 734.8 0 171 120 216 734.8 0 216 121 113 734.8 0 113 122 67 734.8 0 67 123 72 734.8 0 72 124 121 734.8 0 121 125 133 734.8 0 133 126 165 734.8 0 165 127 163 734.8 0 163 128 127 734.8 0 127 129 106 734.8 0 106 130 114 734.8 0 114 131 82 734.8 0 82 132 82 734.8 0 82 133 111 734.8 0 111 134 215 734.8 0 215 135 342 734.8 0 342 136 285 734.8 0 285 137 234 734.8 0 234 138 163 734.8 0 163 139 124 734.8 0 124 140 129 734.8 0 129 141 137 734.8 0 137 142 105 734.8 0 105 143 112 734.8 0 112 144 144 734.8 0 144 145 711 734.8 0 711 146 389 734.8 0 389 147 350 734.8 0 350 148 125 734.8 0 125 149 114 734.8 0 114 150 106 734.8 0 106 151 118 734.8 0 118 152 132 734.8 0 132 153 193 734.8 0 193 154 209 734.8 0 209 155 173 734.8 0 173 156 166 734.8 0 166 157 197 734.8 0 197 158 202 734.8 0 202 159 192 734.8 0 192 160 144 734.8 0 144 161 137 734.8 0 137 162 157 734.8 0 157 163 119 734.8 0 119 164 188 734.8 0 188 165 364 734.8 0 364 166 342 734.8 0 342 167 208 734.8 0 208 168 151 734.8 0 151 169 145 734.8 0 145 170 135 734.8 0 135 171 160 734.8 0 160 172 165 734.8 0 165 173 849 734.8 0 849 174 1857 734.8 0 1857 175 867 734.8 0 867 176 288 734.8 0 288 177 101 734.8 0 101 178 147 734.8 0 147 179 167 734.8 0 167 180 219 734.8 0 219 181 362 734.8 0 362 182 470 734.8 0 470 183 381 734.8 0 381 184 233 734.8 0 233 185 148 734.8 0 148 186 320 734.8 0 320 187 243 734.8 0 243 188 212 734.8 0 212 189 182 734.8 0 182 190 178 734.8 0 178 191 247 734.8 0 247 192 356 734.8 0 356 193 417 734.8 0 417 194 227 734.8 0 227 195 109 734.8 0 109 196 93 734.8 0 93 197 152 734.8 0 152 198 235 734.8 0 235 199 181 734.8 0 181 200 120 734.8 0 120 201 152 734.8 0 152 202 223 734.8 0 223 203 265 734.8 0 265 204 285 734.8 0 285 205 225 734.8 0 225 206 300 734.8 0 300 207 244 734.8 0 244 208 198 734.8 0 198 209 128 734.8 0 128 210 139 734.8 0 139 211 178 734.8 0 178 212 200 734.8 0 200 213 222 734.8 0 222 214 296 734.8 0 296 215 237 734.8 0 237 216 228 734.8 0 228 217 227 734.8 0 227 218 292 734.8 0 292 219 334 734.8 0 334 220 343 734.8 0 343 221 317 734.8 0 317 222 323 734.8 0 323 223 282 734.8 0 282 224 345 734.8 0 345 225 4139 734.8 0 4139 226 8707 734.8 0 8707 227 1486 734.8 0 1486 228 249 734.8 0 249 229 335 734.8 0 335 230 842 734.8 0 842 231 1173 734.8 0 1173 232 1016 734.8 0 1016 233 589 734.8 0 589 234 379 734.8 0 379 235 332 734.8 0 332 236 210 734.8 0 210 237 252 734.8 0 252 238 221 734.8 0 221 239 287 734.8 0 287 240 201 734.8 0 201 241 214 734.8 0 214 242 261 734.8 0 261 243 224 734.8 0 224 244 207 734.8 0 207 245 201 734.8 0 201 246 451 734.8 0 451 247 425 734.8 0 425 248 223 734.8 0 223 249 93 734.8 0 93 250 2822 734.8 0 2822 251 4016 734.8 0 4016 252 2420 734.8 0 2420 253 820 734.8 0 820 254 643 734.8 0 643 255 297 734.8 0 297 256 277 734.8 0 277 257 243 734.8 0 243 258 252 734.8 0 252 259 158 734.8 0 158 260 203 734.8 0 203 261 231 734.8 0 231 262 226 734.8 0 226 263 236 734.8 0 236 264 226 734.8 0 226 265 693 734.8 0 693 266 477 734.8 0 477 267 359 734.8 0 359 268 213 734.8 0 213 269 183 734.8 0 183 270 236 734.8 0 236 271 664 734.8 0 664 272 1262 734.8 0 1262 273 362 734.8 0 362 274 419 734.8 0 419 275 900 734.8 0 900 276 556 734.8 0 556 277 314 734.8 0 314 278 381 734.8 0 381 279 578 734.8 0 578 280 1157 734.8 0 1157 281 999 734.8 0 999 282 680 734.8 0 680 283 467 734.8 0 467 284 703 734.8 0 703 285 1610 734.8 0 1610 286 1124 734.8 0 1124 287 610 734.8 0 610 288 544 734.8 0 544 289 532 734.8 0 532 290 1373 734.8 0 1373 291 1239 734.8 0 1239 292 985 734.8 0 985 293 541 734.8 0 541 294 545 734.8 0 545 295 915 734.8 0 915 296 1421 734.8 0 1421 297 2101 734.8 0 2101 298 3189 734.8 0 3189 299 5373 734.8 0 5373 300 9026 734.8 0 9026 301 35245 734.8 0 35245 Finished in 3.55 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 74,222 Reads with adapters: 5,993 (8.1%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 5,982 (8.1%) Total basepairs processed: 18,231,050 bp Total written (filtered): 1,565,641 bp (8.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5993 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 22 0.0 3 0 0 0 0 22 18 2 0.0 3 0 0 2 19 79 0.0 3 0 78 0 0 1 20 5272 0.0 4 5194 77 1 21 16 0.0 4 2 13 0 1 22 1 0.0 4 0 0 0 0 1 23 2 0.0 4 0 0 0 2 32 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 2 87 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 1 1 109 5 0.0 4 0 0 0 4 1 111 24 0.0 4 0 0 0 24 121 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 540 0.0 4 0 0 0 0 540 128 3 0.0 4 0 0 0 0 3 144 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 155 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 5,982 Reads with adapters: 146 (2.4%) Reads written (passing filters): 5,982 (100.0%) Total basepairs processed: 1,565,641 bp Total written (filtered): 1,562,940 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 146 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 2.7% G: 92.5% T: 2.7% none/other: 0.7% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 2 23.4 0 2 9 14 0.0 0 1 13 10 103 0.0 1 5 98 11 21 0.0 1 0 21 138 1 0.0 1 1 223 2 0.0 1 2 224 1 0.0 1 1 226 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.