This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.63 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 85,041 (2.8%) Reads that were too short: 20,726 (0.7%) Reads written (passing filters): 64,315 (2.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 17,071,682 bp (1.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 85041 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 608 734.8 0 608 7 42659 734.8 0 42659 8 3655 734.8 0 3655 9 55 734.8 0 55 10 46 734.8 0 46 11 43 734.8 0 43 12 36 734.8 0 36 13 39 734.8 0 39 14 43 734.8 0 43 15 39 734.8 0 39 16 37 734.8 0 37 17 39 734.8 0 39 18 41 734.8 0 41 19 44 734.8 0 44 20 44 734.8 0 44 21 59 734.8 0 59 22 49 734.8 0 49 23 98 734.8 0 98 24 32 734.8 0 32 25 21 734.8 0 21 26 34 734.8 0 34 27 33 734.8 0 33 28 45 734.8 0 45 29 51 734.8 0 51 30 55 734.8 0 55 31 88 734.8 0 88 32 36 734.8 0 36 33 44 734.8 0 44 34 38 734.8 0 38 35 46 734.8 0 46 36 35 734.8 0 35 37 39 734.8 0 39 38 52 734.8 0 52 39 206 734.8 0 206 40 95 734.8 0 95 41 39 734.8 0 39 42 37 734.8 0 37 43 59 734.8 0 59 44 31 734.8 0 31 45 27 734.8 0 27 46 41 734.8 0 41 47 29 734.8 0 29 48 40 734.8 0 40 49 41 734.8 0 41 50 42 734.8 0 42 51 38 734.8 0 38 52 38 734.8 0 38 53 48 734.8 0 48 54 48 734.8 0 48 55 26 734.8 0 26 56 22 734.8 0 22 57 50 734.8 0 50 58 43 734.8 0 43 59 27 734.8 0 27 60 38 734.8 0 38 61 49 734.8 0 49 62 37 734.8 0 37 63 36 734.8 0 36 64 90 734.8 0 90 65 3124 734.8 0 3124 66 146 734.8 0 146 67 46 734.8 0 46 68 37 734.8 0 37 69 36 734.8 0 36 70 64 734.8 0 64 71 69 734.8 0 69 72 49 734.8 0 49 73 40 734.8 0 40 74 42 734.8 0 42 75 39 734.8 0 39 76 30 734.8 0 30 77 38 734.8 0 38 78 34 734.8 0 34 79 46 734.8 0 46 80 45 734.8 0 45 81 54 734.8 0 54 82 58 734.8 0 58 83 50 734.8 0 50 84 41 734.8 0 41 85 88 734.8 0 88 86 95 734.8 0 95 87 42 734.8 0 42 88 30 734.8 0 30 89 38 734.8 0 38 90 36 734.8 0 36 91 43 734.8 0 43 92 51 734.8 0 51 93 44 734.8 0 44 94 38 734.8 0 38 95 61 734.8 0 61 96 92 734.8 0 92 97 69 734.8 0 69 98 207 734.8 0 207 99 187 734.8 0 187 100 78 734.8 0 78 101 79 734.8 0 79 102 119 734.8 0 119 103 125 734.8 0 125 104 83 734.8 0 83 105 57 734.8 0 57 106 35 734.8 0 35 107 56 734.8 0 56 108 34 734.8 0 34 109 40 734.8 0 40 110 66 734.8 0 66 111 84 734.8 0 84 112 71 734.8 0 71 113 73 734.8 0 73 114 50 734.8 0 50 115 53 734.8 0 53 116 74 734.8 0 74 117 92 734.8 0 92 118 64 734.8 0 64 119 52 734.8 0 52 120 64 734.8 0 64 121 48 734.8 0 48 122 47 734.8 0 47 123 96 734.8 0 96 124 133 734.8 0 133 125 116 734.8 0 116 126 63 734.8 0 63 127 72 734.8 0 72 128 46 734.8 0 46 129 64 734.8 0 64 130 56 734.8 0 56 131 83 734.8 0 83 132 108 734.8 0 108 133 163 734.8 0 163 134 72 734.8 0 72 135 105 734.8 0 105 136 70 734.8 0 70 137 89 734.8 0 89 138 132 734.8 0 132 139 128 734.8 0 128 140 63 734.8 0 63 141 59 734.8 0 59 142 73 734.8 0 73 143 148 734.8 0 148 144 143 734.8 0 143 145 113 734.8 0 113 146 69 734.8 0 69 147 126 734.8 0 126 148 95 734.8 0 95 149 90 734.8 0 90 150 75 734.8 0 75 151 72 734.8 0 72 152 58 734.8 0 58 153 75 734.8 0 75 154 60 734.8 0 60 155 60 734.8 0 60 156 63 734.8 0 63 157 73 734.8 0 73 158 131 734.8 0 131 159 157 734.8 0 157 160 114 734.8 0 114 161 91 734.8 0 91 162 109 734.8 0 109 163 68 734.8 0 68 164 72 734.8 0 72 165 55 734.8 0 55 166 74 734.8 0 74 167 137 734.8 0 137 168 114 734.8 0 114 169 128 734.8 0 128 170 156 734.8 0 156 171 184 734.8 0 184 172 136 734.8 0 136 173 77 734.8 0 77 174 74 734.8 0 74 175 59 734.8 0 59 176 64 734.8 0 64 177 147 734.8 0 147 178 153 734.8 0 153 179 141 734.8 0 141 180 122 734.8 0 122 181 78 734.8 0 78 182 85 734.8 0 85 183 118 734.8 0 118 184 232 734.8 0 232 185 127 734.8 0 127 186 82 734.8 0 82 187 178 734.8 0 178 188 150 734.8 0 150 189 137 734.8 0 137 190 104 734.8 0 104 191 54 734.8 0 54 192 96 734.8 0 96 193 83 734.8 0 83 194 132 734.8 0 132 195 98 734.8 0 98 196 94 734.8 0 94 197 97 734.8 0 97 198 135 734.8 0 135 199 143 734.8 0 143 200 48 734.8 0 48 201 57 734.8 0 57 202 51 734.8 0 51 203 50 734.8 0 50 204 95 734.8 0 95 205 73 734.8 0 73 206 92 734.8 0 92 207 96 734.8 0 96 208 56 734.8 0 56 209 61 734.8 0 61 210 125 734.8 0 125 211 169 734.8 0 169 212 139 734.8 0 139 213 112 734.8 0 112 214 540 734.8 0 540 215 556 734.8 0 556 216 379 734.8 0 379 217 109 734.8 0 109 218 84 734.8 0 84 219 96 734.8 0 96 220 74 734.8 0 74 221 54 734.8 0 54 222 69 734.8 0 69 223 305 734.8 0 305 224 620 734.8 0 620 225 242 734.8 0 242 226 77 734.8 0 77 227 94 734.8 0 94 228 111 734.8 0 111 229 160 734.8 0 160 230 71 734.8 0 71 231 62 734.8 0 62 232 50 734.8 0 50 233 55 734.8 0 55 234 58 734.8 0 58 235 56 734.8 0 56 236 83 734.8 0 83 237 83 734.8 0 83 238 141 734.8 0 141 239 180 734.8 0 180 240 172 734.8 0 172 241 131 734.8 0 131 242 63 734.8 0 63 243 50 734.8 0 50 244 45 734.8 0 45 245 88 734.8 0 88 246 152 734.8 0 152 247 212 734.8 0 212 248 106 734.8 0 106 249 59 734.8 0 59 250 25 734.8 0 25 251 44 734.8 0 44 252 46 734.8 0 46 253 58 734.8 0 58 254 67 734.8 0 67 255 140 734.8 0 140 256 108 734.8 0 108 257 141 734.8 0 141 258 174 734.8 0 174 259 118 734.8 0 118 260 161 734.8 0 161 261 54 734.8 0 54 262 59 734.8 0 59 263 144 734.8 0 144 264 216 734.8 0 216 265 159 734.8 0 159 266 106 734.8 0 106 267 227 734.8 0 227 268 224 734.8 0 224 269 246 734.8 0 246 270 126 734.8 0 126 271 197 734.8 0 197 272 3370 734.8 0 3370 273 2942 734.8 0 2942 274 1222 734.8 0 1222 275 85 734.8 0 85 276 104 734.8 0 104 277 116 734.8 0 116 278 113 734.8 0 113 279 233 734.8 0 233 280 77 734.8 0 77 281 35 734.8 0 35 282 55 734.8 0 55 283 120 734.8 0 120 284 173 734.8 0 173 285 199 734.8 0 199 286 181 734.8 0 181 287 209 734.8 0 209 288 118 734.8 0 118 289 41 734.8 0 41 290 36 734.8 0 36 291 42 734.8 0 42 292 58 734.8 0 58 293 131 734.8 0 131 294 109 734.8 0 109 295 84 734.8 0 84 296 52 734.8 0 52 297 55 734.8 0 55 298 58 734.8 0 58 299 137 734.8 0 137 300 253 734.8 0 253 301 872 734.8 0 872 Finished in 3.22 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 64,315 Reads with adapters: 4,248 (6.6%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 4,239 (6.6%) Total basepairs processed: 17,071,682 bp Total written (filtered): 1,097,348 bp (6.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4248 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 3 1 19 51 0.0 3 0 51 20 3603 0.0 4 3560 41 1 0 1 21 11 0.0 4 4 7 22 2 0.0 4 0 1 1 23 1 0.0 4 0 0 0 1 31 1 0.0 4 0 0 0 0 1 36 10 0.0 4 0 0 0 0 10 37 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 543 0.0 4 0 0 0 0 543 128 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 4,239 Reads with adapters: 119 (2.8%) Reads written (passing filters): 4,239 (100.0%) Total basepairs processed: 1,097,348 bp Total written (filtered): 1,095,103 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 119 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 0.0% G: 97.5% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 16.6 0 1 10 20 0.0 1 0 20 11 94 0.0 1 0 94 232 1 0.0 1 1 258 2 0.0 1 2 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.