This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 81.27 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 375,564 (12.5%) Reads that were too short: 49,190 (1.6%) Reads written (passing filters): 326,374 (10.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 87,496,254 bp (9.7%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 375564 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2459 734.8 0 2459 7 257844 734.8 0 257844 8 8284 734.8 0 8284 9 28 734.8 0 28 10 33 734.8 0 33 11 26 734.8 0 26 12 96 734.8 0 96 13 286 734.8 0 286 14 153 734.8 0 153 15 68 734.8 0 68 16 22 734.8 0 22 17 22 734.8 0 22 18 6 734.8 0 6 19 15 734.8 0 15 20 10 734.8 0 10 21 11 734.8 0 11 22 13 734.8 0 13 23 21 734.8 0 21 24 21 734.8 0 21 25 38 734.8 0 38 26 47 734.8 0 47 27 34 734.8 0 34 28 15 734.8 0 15 29 18 734.8 0 18 30 16 734.8 0 16 31 10 734.8 0 10 32 6 734.8 0 6 33 21 734.8 0 21 34 23 734.8 0 23 35 8 734.8 0 8 36 16 734.8 0 16 37 8 734.8 0 8 38 75 734.8 0 75 39 22 734.8 0 22 40 14 734.8 0 14 41 6 734.8 0 6 42 45 734.8 0 45 43 12 734.8 0 12 44 15 734.8 0 15 45 14 734.8 0 14 46 12 734.8 0 12 47 18 734.8 0 18 48 12 734.8 0 12 49 20 734.8 0 20 50 16 734.8 0 16 51 18 734.8 0 18 52 10 734.8 0 10 53 22 734.8 0 22 54 24 734.8 0 24 55 18 734.8 0 18 56 14 734.8 0 14 57 26 734.8 0 26 58 4 734.8 0 4 59 17 734.8 0 17 60 38 734.8 0 38 61 50 734.8 0 50 62 18 734.8 0 18 63 22 734.8 0 22 64 15 734.8 0 15 65 11 734.8 0 11 66 15 734.8 0 15 67 11 734.8 0 11 68 15 734.8 0 15 69 13 734.8 0 13 70 21 734.8 0 21 71 62 734.8 0 62 72 15 734.8 0 15 73 28 734.8 0 28 74 32 734.8 0 32 75 101 734.8 0 101 76 146 734.8 0 146 77 63 734.8 0 63 78 125 734.8 0 125 79 107 734.8 0 107 80 206 734.8 0 206 81 209 734.8 0 209 82 485 734.8 0 485 83 277 734.8 0 277 84 149 734.8 0 149 85 253 734.8 0 253 86 117 734.8 0 117 87 305 734.8 0 305 88 482 734.8 0 482 89 556 734.8 0 556 90 345 734.8 0 345 91 327 734.8 0 327 92 62 734.8 0 62 93 191 734.8 0 191 94 391 734.8 0 391 95 271 734.8 0 271 96 270 734.8 0 270 97 245 734.8 0 245 98 223 734.8 0 223 99 175 734.8 0 175 100 154 734.8 0 154 101 134 734.8 0 134 102 289 734.8 0 289 103 367 734.8 0 367 104 149 734.8 0 149 105 175 734.8 0 175 106 187 734.8 0 187 107 197 734.8 0 197 108 66 734.8 0 66 109 79 734.8 0 79 110 133 734.8 0 133 111 319 734.8 0 319 112 379 734.8 0 379 113 279 734.8 0 279 114 315 734.8 0 315 115 198 734.8 0 198 116 150 734.8 0 150 117 140 734.8 0 140 118 310 734.8 0 310 119 392 734.8 0 392 120 414 734.8 0 414 121 294 734.8 0 294 122 240 734.8 0 240 123 326 734.8 0 326 124 312 734.8 0 312 125 226 734.8 0 226 126 344 734.8 0 344 127 340 734.8 0 340 128 267 734.8 0 267 129 250 734.8 0 250 130 239 734.8 0 239 131 199 734.8 0 199 132 272 734.8 0 272 133 320 734.8 0 320 134 310 734.8 0 310 135 288 734.8 0 288 136 238 734.8 0 238 137 285 734.8 0 285 138 315 734.8 0 315 139 452 734.8 0 452 140 652 734.8 0 652 141 441 734.8 0 441 142 236 734.8 0 236 143 217 734.8 0 217 144 349 734.8 0 349 145 311 734.8 0 311 146 364 734.8 0 364 147 322 734.8 0 322 148 312 734.8 0 312 149 184 734.8 0 184 150 229 734.8 0 229 151 272 734.8 0 272 152 308 734.8 0 308 153 354 734.8 0 354 154 320 734.8 0 320 155 328 734.8 0 328 156 291 734.8 0 291 157 304 734.8 0 304 158 401 734.8 0 401 159 439 734.8 0 439 160 1159 734.8 0 1159 161 1283 734.8 0 1283 162 594 734.8 0 594 163 344 734.8 0 344 164 306 734.8 0 306 165 278 734.8 0 278 166 197 734.8 0 197 167 221 734.8 0 221 168 322 734.8 0 322 169 369 734.8 0 369 170 285 734.8 0 285 171 245 734.8 0 245 172 272 734.8 0 272 173 365 734.8 0 365 174 289 734.8 0 289 175 270 734.8 0 270 176 327 734.8 0 327 177 368 734.8 0 368 178 303 734.8 0 303 179 294 734.8 0 294 180 520 734.8 0 520 181 572 734.8 0 572 182 667 734.8 0 667 183 419 734.8 0 419 184 400 734.8 0 400 185 502 734.8 0 502 186 663 734.8 0 663 187 6076 734.8 0 6076 188 9500 734.8 0 9500 189 1710 734.8 0 1710 190 367 734.8 0 367 191 289 734.8 0 289 192 225 734.8 0 225 193 324 734.8 0 324 194 204 734.8 0 204 195 266 734.8 0 266 196 414 734.8 0 414 197 471 734.8 0 471 198 249 734.8 0 249 199 236 734.8 0 236 200 257 734.8 0 257 201 416 734.8 0 416 202 801 734.8 0 801 203 496 734.8 0 496 204 366 734.8 0 366 205 313 734.8 0 313 206 528 734.8 0 528 207 476 734.8 0 476 208 375 734.8 0 375 209 362 734.8 0 362 210 477 734.8 0 477 211 606 734.8 0 606 212 425 734.8 0 425 213 383 734.8 0 383 214 378 734.8 0 378 215 334 734.8 0 334 216 324 734.8 0 324 217 286 734.8 0 286 218 315 734.8 0 315 219 429 734.8 0 429 220 467 734.8 0 467 221 453 734.8 0 453 222 469 734.8 0 469 223 395 734.8 0 395 224 332 734.8 0 332 225 320 734.8 0 320 226 310 734.8 0 310 227 375 734.8 0 375 228 328 734.8 0 328 229 371 734.8 0 371 230 752 734.8 0 752 231 897 734.8 0 897 232 544 734.8 0 544 233 360 734.8 0 360 234 422 734.8 0 422 235 318 734.8 0 318 236 176 734.8 0 176 237 269 734.8 0 269 238 420 734.8 0 420 239 487 734.8 0 487 240 608 734.8 0 608 241 678 734.8 0 678 242 499 734.8 0 499 243 332 734.8 0 332 244 309 734.8 0 309 245 374 734.8 0 374 246 349 734.8 0 349 247 228 734.8 0 228 248 238 734.8 0 238 249 159 734.8 0 159 250 392 734.8 0 392 251 510 734.8 0 510 252 464 734.8 0 464 253 349 734.8 0 349 254 360 734.8 0 360 255 294 734.8 0 294 256 403 734.8 0 403 257 315 734.8 0 315 258 289 734.8 0 289 259 260 734.8 0 260 260 267 734.8 0 267 261 429 734.8 0 429 262 974 734.8 0 974 263 344 734.8 0 344 264 351 734.8 0 351 265 304 734.8 0 304 266 394 734.8 0 394 267 286 734.8 0 286 268 273 734.8 0 273 269 338 734.8 0 338 270 531 734.8 0 531 271 455 734.8 0 455 272 378 734.8 0 378 273 540 734.8 0 540 274 459 734.8 0 459 275 517 734.8 0 517 276 1151 734.8 0 1151 277 752 734.8 0 752 278 598 734.8 0 598 279 647 734.8 0 647 280 481 734.8 0 481 281 406 734.8 0 406 282 497 734.8 0 497 283 893 734.8 0 893 284 858 734.8 0 858 285 566 734.8 0 566 286 268 734.8 0 268 287 252 734.8 0 252 288 478 734.8 0 478 289 756 734.8 0 756 290 499 734.8 0 499 291 413 734.8 0 413 292 457 734.8 0 457 293 389 734.8 0 389 294 538 734.8 0 538 295 536 734.8 0 536 296 622 734.8 0 622 297 402 734.8 0 402 298 550 734.8 0 550 299 582 734.8 0 582 300 893 734.8 0 893 301 4317 734.8 0 4317 Finished in 12.77 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 326,374 Reads with adapters: 259,110 (79.4%) Reads that were too short: 77 (0.0%) Reads written (passing filters): 259,033 (79.4%) Total basepairs processed: 87,496,254 bp Total written (filtered): 71,747,534 bp (82.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 259110 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 15 0.0 2 0 0 0 15 15 70 0.0 3 0 0 56 14 16 2382 0.0 3 0 2187 130 65 17 255309 0.0 3 248052 6170 852 235 18 738 0.0 3 49 601 40 48 19 216 0.0 3 1 1 131 83 20 247 0.0 3 0 3 0 244 21 2 0.0 3 1 0 0 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 1 26 1 0.0 3 1 30 5 0.0 3 3 0 0 2 37 2 0.0 3 0 0 0 2 38 2 0.0 3 1 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 90 9 0.0 3 0 0 0 9 99 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 0 4 101 7 0.0 3 0 0 0 7 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 10 0.0 3 0 0 2 8 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 1 141 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 3 0.0 3 0 0 1 2 192 10 0.0 3 0 0 0 10 206 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 3 0.0 3 0 0 0 3 279 2 0.0 3 0 0 1 1 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.24 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 259,033 Reads with adapters: 23,662 (9.1%) Reads written (passing filters): 259,033 (100.0%) Total basepairs processed: 71,747,534 bp Total written (filtered): 71,676,465 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 23662 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.7% C: 39.9% G: 4.5% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 23592 4047.4 0 23592 4 58 1011.8 0 58 5 11 253.0 0 11 6 1 63.2 0 1