This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 80.14 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 420,248 (14.0%) Reads that were too short: 33,280 (1.1%) Reads written (passing filters): 386,968 (12.9%) Total basepairs processed: 905,907,359 bp Total written (filtered): 103,624,318 bp (11.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 420248 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3028 734.8 0 3028 7 264343 734.8 0 264343 8 900 734.8 0 900 9 38026 734.8 0 38026 10 167 734.8 0 167 11 86 734.8 0 86 12 92 734.8 0 92 13 68 734.8 0 68 14 77 734.8 0 77 15 87 734.8 0 87 16 94 734.8 0 94 17 111 734.8 0 111 18 96 734.8 0 96 19 98 734.8 0 98 20 84 734.8 0 84 21 146 734.8 0 146 22 111 734.8 0 111 23 98 734.8 0 98 24 127 734.8 0 127 25 297 734.8 0 297 26 134 734.8 0 134 27 76 734.8 0 76 28 79 734.8 0 79 29 74 734.8 0 74 30 94 734.8 0 94 31 58 734.8 0 58 32 93 734.8 0 93 33 87 734.8 0 87 34 82 734.8 0 82 35 69 734.8 0 69 36 78 734.8 0 78 37 65 734.8 0 65 38 69 734.8 0 69 39 84 734.8 0 84 40 83 734.8 0 83 41 79 734.8 0 79 42 82 734.8 0 82 43 85 734.8 0 85 44 73 734.8 0 73 45 78 734.8 0 78 46 82 734.8 0 82 47 81 734.8 0 81 48 83 734.8 0 83 49 68 734.8 0 68 50 68 734.8 0 68 51 93 734.8 0 93 52 70 734.8 0 70 53 86 734.8 0 86 54 95 734.8 0 95 55 156 734.8 0 156 56 73 734.8 0 73 57 80 734.8 0 80 58 92 734.8 0 92 59 110 734.8 0 110 60 91 734.8 0 91 61 173 734.8 0 173 62 206 734.8 0 206 63 104 734.8 0 104 64 86 734.8 0 86 65 98 734.8 0 98 66 360 734.8 0 360 67 88 734.8 0 88 68 65 734.8 0 65 69 108 734.8 0 108 70 138 734.8 0 138 71 96 734.8 0 96 72 120 734.8 0 120 73 89 734.8 0 89 74 90 734.8 0 90 75 76 734.8 0 76 76 104 734.8 0 104 77 128 734.8 0 128 78 146 734.8 0 146 79 141 734.8 0 141 80 190 734.8 0 190 81 224 734.8 0 224 82 331 734.8 0 331 83 635 734.8 0 635 84 369 734.8 0 369 85 142 734.8 0 142 86 118 734.8 0 118 87 103 734.8 0 103 88 124 734.8 0 124 89 98 734.8 0 98 90 135 734.8 0 135 91 175 734.8 0 175 92 206 734.8 0 206 93 158 734.8 0 158 94 277 734.8 0 277 95 189 734.8 0 189 96 166 734.8 0 166 97 226 734.8 0 226 98 202 734.8 0 202 99 278 734.8 0 278 100 233 734.8 0 233 101 266 734.8 0 266 102 186 734.8 0 186 103 154 734.8 0 154 104 140 734.8 0 140 105 191 734.8 0 191 106 190 734.8 0 190 107 239 734.8 0 239 108 224 734.8 0 224 109 315 734.8 0 315 110 391 734.8 0 391 111 483 734.8 0 483 112 7308 734.8 0 7308 113 15791 734.8 0 15791 114 1333 734.8 0 1333 115 214 734.8 0 214 116 189 734.8 0 189 117 341 734.8 0 341 118 341 734.8 0 341 119 426 734.8 0 426 120 703 734.8 0 703 121 1115 734.8 0 1115 122 351 734.8 0 351 123 241 734.8 0 241 124 248 734.8 0 248 125 416 734.8 0 416 126 1238 734.8 0 1238 127 304 734.8 0 304 128 244 734.8 0 244 129 211 734.8 0 211 130 259 734.8 0 259 131 299 734.8 0 299 132 264 734.8 0 264 133 247 734.8 0 247 134 254 734.8 0 254 135 249 734.8 0 249 136 444 734.8 0 444 137 245 734.8 0 245 138 280 734.8 0 280 139 318 734.8 0 318 140 322 734.8 0 322 141 236 734.8 0 236 142 345 734.8 0 345 143 337 734.8 0 337 144 300 734.8 0 300 145 280 734.8 0 280 146 314 734.8 0 314 147 507 734.8 0 507 148 621 734.8 0 621 149 458 734.8 0 458 150 354 734.8 0 354 151 325 734.8 0 325 152 404 734.8 0 404 153 268 734.8 0 268 154 310 734.8 0 310 155 295 734.8 0 295 156 228 734.8 0 228 157 301 734.8 0 301 158 280 734.8 0 280 159 291 734.8 0 291 160 237 734.8 0 237 161 248 734.8 0 248 162 284 734.8 0 284 163 372 734.8 0 372 164 319 734.8 0 319 165 343 734.8 0 343 166 585 734.8 0 585 167 602 734.8 0 602 168 391 734.8 0 391 169 477 734.8 0 477 170 431 734.8 0 431 171 952 734.8 0 952 172 1359 734.8 0 1359 173 1008 734.8 0 1008 174 562 734.8 0 562 175 417 734.8 0 417 176 315 734.8 0 315 177 353 734.8 0 353 178 349 734.8 0 349 179 313 734.8 0 313 180 364 734.8 0 364 181 351 734.8 0 351 182 371 734.8 0 371 183 878 734.8 0 878 184 2209 734.8 0 2209 185 541 734.8 0 541 186 287 734.8 0 287 187 379 734.8 0 379 188 292 734.8 0 292 189 326 734.8 0 326 190 440 734.8 0 440 191 2566 734.8 0 2566 192 1961 734.8 0 1961 193 834 734.8 0 834 194 547 734.8 0 547 195 384 734.8 0 384 196 322 734.8 0 322 197 477 734.8 0 477 198 398 734.8 0 398 199 904 734.8 0 904 200 443 734.8 0 443 201 268 734.8 0 268 202 305 734.8 0 305 203 325 734.8 0 325 204 289 734.8 0 289 205 262 734.8 0 262 206 236 734.8 0 236 207 270 734.8 0 270 208 284 734.8 0 284 209 252 734.8 0 252 210 248 734.8 0 248 211 327 734.8 0 327 212 320 734.8 0 320 213 306 734.8 0 306 214 265 734.8 0 265 215 405 734.8 0 405 216 417 734.8 0 417 217 319 734.8 0 319 218 326 734.8 0 326 219 361 734.8 0 361 220 362 734.8 0 362 221 219 734.8 0 219 222 224 734.8 0 224 223 347 734.8 0 347 224 343 734.8 0 343 225 302 734.8 0 302 226 343 734.8 0 343 227 412 734.8 0 412 228 295 734.8 0 295 229 556 734.8 0 556 230 318 734.8 0 318 231 262 734.8 0 262 232 262 734.8 0 262 233 287 734.8 0 287 234 270 734.8 0 270 235 391 734.8 0 391 236 294 734.8 0 294 237 281 734.8 0 281 238 275 734.8 0 275 239 295 734.8 0 295 240 411 734.8 0 411 241 521 734.8 0 521 242 391 734.8 0 391 243 489 734.8 0 489 244 362 734.8 0 362 245 368 734.8 0 368 246 395 734.8 0 395 247 279 734.8 0 279 248 144 734.8 0 144 249 145 734.8 0 145 250 164 734.8 0 164 251 440 734.8 0 440 252 1360 734.8 0 1360 253 483 734.8 0 483 254 294 734.8 0 294 255 357 734.8 0 357 256 370 734.8 0 370 257 317 734.8 0 317 258 210 734.8 0 210 259 266 734.8 0 266 260 202 734.8 0 202 261 263 734.8 0 263 262 320 734.8 0 320 263 317 734.8 0 317 264 278 734.8 0 278 265 311 734.8 0 311 266 249 734.8 0 249 267 674 734.8 0 674 268 960 734.8 0 960 269 256 734.8 0 256 270 224 734.8 0 224 271 289 734.8 0 289 272 325 734.8 0 325 273 277 734.8 0 277 274 268 734.8 0 268 275 271 734.8 0 271 276 261 734.8 0 261 277 275 734.8 0 275 278 348 734.8 0 348 279 269 734.8 0 269 280 292 734.8 0 292 281 224 734.8 0 224 282 194 734.8 0 194 283 613 734.8 0 613 284 357 734.8 0 357 285 220 734.8 0 220 286 244 734.8 0 244 287 281 734.8 0 281 288 230 734.8 0 230 289 245 734.8 0 245 290 356 734.8 0 356 291 417 734.8 0 417 292 413 734.8 0 413 293 407 734.8 0 407 294 286 734.8 0 286 295 227 734.8 0 227 296 344 734.8 0 344 297 468 734.8 0 468 298 335 734.8 0 335 299 389 734.8 0 389 300 393 734.8 0 393 301 87 734.8 0 87 Finished in 16.20 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 386,968 Reads with adapters: 266,987 (69.0%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 266,965 (69.0%) Total basepairs processed: 103,624,318 bp Total written (filtered): 73,116,698 bp (70.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 266987 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 26 0.0 3 0 0 0 2 24 18 82 0.0 3 0 0 79 3 19 2358 0.0 3 0 2291 61 3 3 20 263288 0.0 4 259827 3326 105 18 12 21 715 0.0 4 152 554 7 2 22 54 0.0 4 4 2 40 1 7 23 105 0.0 4 5 2 2 90 6 24 6 0.0 4 3 0 0 2 1 25 2 0.0 4 1 0 0 1 27 1 0.0 4 0 0 1 28 1 0.0 4 0 0 0 1 29 2 0.0 4 1 0 0 0 1 31 1 0.0 4 1 33 1 0.0 4 0 0 1 39 1 0.0 4 1 41 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 51 9 0.0 4 0 0 0 0 9 54 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 110 3 0.0 4 0 0 0 0 3 111 3 0.0 4 0 0 0 3 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 291 0.0 4 0 0 0 0 291 128 6 0.0 4 0 0 0 0 6 136 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 151 3 0.0 4 0 0 0 0 3 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 211 1 0.0 4 1 214 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.03 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 266,965 Reads with adapters: 5,001 (1.9%) Reads written (passing filters): 266,965 (100.0%) Total basepairs processed: 73,116,698 bp Total written (filtered): 72,919,244 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5001 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 12.6% G: 84.3% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 34 4171.3 0 34 4 14 1042.8 0 14 5 108 260.7 0 108 6 3 65.2 0 3 10 13 0.3 1 1 12 11 4051 0.1 1 17 4034 12 107 0.0 1 1 106 13 5 0.0 1 4 1 14 1 0.0 1 0 1 15 1 0.0 1 0 1 74 1 0.0 1 1 113 1 0.0 1 1 151 1 0.0 1 0 1 152 8 0.0 1 0 8 153 1 0.0 1 0 1 172 1 0.0 1 1 191 2 0.0 1 0 2 193 1 0.0 1 0 1 210 9 0.0 1 1 8 224 3 0.0 1 3 225 556 0.0 1 516 40 226 15 0.0 1 15 238 1 0.0 1 1 259 2 0.0 1 2 262 14 0.0 1 13 1 263 1 0.0 1 1 265 44 0.0 1 41 3 271 2 0.0 1 2 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.