This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.35 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 99,056 (3.3%) Reads that were too short: 30,512 (1.0%) Reads written (passing filters): 68,544 (2.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 14,261,970 bp (1.6%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 99056 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1074 734.8 0 1074 7 19627 734.8 0 19627 8 2424 734.8 0 2424 9 3724 734.8 0 3724 10 36 734.8 0 36 11 22 734.8 0 22 12 17 734.8 0 17 13 23 734.8 0 23 14 26 734.8 0 26 15 15 734.8 0 15 16 22 734.8 0 22 17 17 734.8 0 17 18 12 734.8 0 12 19 29 734.8 0 29 20 22 734.8 0 22 21 10 734.8 0 10 22 24 734.8 0 24 23 12 734.8 0 12 24 16 734.8 0 16 25 20 734.8 0 20 26 26 734.8 0 26 27 40 734.8 0 40 28 30 734.8 0 30 29 26 734.8 0 26 30 20 734.8 0 20 31 15 734.8 0 15 32 24 734.8 0 24 33 20 734.8 0 20 34 16 734.8 0 16 35 32 734.8 0 32 36 44 734.8 0 44 37 41 734.8 0 41 38 70 734.8 0 70 39 42 734.8 0 42 40 33 734.8 0 33 41 24 734.8 0 24 42 78 734.8 0 78 43 22 734.8 0 22 44 16 734.8 0 16 45 20 734.8 0 20 46 26 734.8 0 26 47 25 734.8 0 25 48 13 734.8 0 13 49 21 734.8 0 21 50 25 734.8 0 25 51 35 734.8 0 35 52 21 734.8 0 21 53 210 734.8 0 210 54 213 734.8 0 213 55 28 734.8 0 28 56 20 734.8 0 20 57 123 734.8 0 123 58 13 734.8 0 13 59 19 734.8 0 19 60 44 734.8 0 44 61 14 734.8 0 14 62 16 734.8 0 16 63 19 734.8 0 19 64 19 734.8 0 19 65 17 734.8 0 17 66 46 734.8 0 46 67 37 734.8 0 37 68 17 734.8 0 17 69 28 734.8 0 28 70 32 734.8 0 32 71 82 734.8 0 82 72 25 734.8 0 25 73 23 734.8 0 23 74 23 734.8 0 23 75 17 734.8 0 17 76 26 734.8 0 26 77 27 734.8 0 27 78 37 734.8 0 37 79 44 734.8 0 44 80 37 734.8 0 37 81 41 734.8 0 41 82 86 734.8 0 86 83 47 734.8 0 47 84 42 734.8 0 42 85 84 734.8 0 84 86 70 734.8 0 70 87 86 734.8 0 86 88 88 734.8 0 88 89 118 734.8 0 118 90 181 734.8 0 181 91 155 734.8 0 155 92 111 734.8 0 111 93 224 734.8 0 224 94 197 734.8 0 197 95 117 734.8 0 117 96 37 734.8 0 37 97 37 734.8 0 37 98 51 734.8 0 51 99 37 734.8 0 37 100 25 734.8 0 25 101 108 734.8 0 108 102 102 734.8 0 102 103 145 734.8 0 145 104 173 734.8 0 173 105 151 734.8 0 151 106 92 734.8 0 92 107 169 734.8 0 169 108 72 734.8 0 72 109 75 734.8 0 75 110 70 734.8 0 70 111 324 734.8 0 324 112 253 734.8 0 253 113 321 734.8 0 321 114 243 734.8 0 243 115 161 734.8 0 161 116 77 734.8 0 77 117 72 734.8 0 72 118 59 734.8 0 59 119 95 734.8 0 95 120 115 734.8 0 115 121 102 734.8 0 102 122 81 734.8 0 81 123 130 734.8 0 130 124 193 734.8 0 193 125 804 734.8 0 804 126 885 734.8 0 885 127 464 734.8 0 464 128 100 734.8 0 100 129 140 734.8 0 140 130 185 734.8 0 185 131 143 734.8 0 143 132 138 734.8 0 138 133 187 734.8 0 187 134 270 734.8 0 270 135 145 734.8 0 145 136 167 734.8 0 167 137 194 734.8 0 194 138 234 734.8 0 234 139 303 734.8 0 303 140 198 734.8 0 198 141 167 734.8 0 167 142 128 734.8 0 128 143 186 734.8 0 186 144 331 734.8 0 331 145 422 734.8 0 422 146 340 734.8 0 340 147 246 734.8 0 246 148 172 734.8 0 172 149 208 734.8 0 208 150 436 734.8 0 436 151 669 734.8 0 669 152 398 734.8 0 398 153 258 734.8 0 258 154 347 734.8 0 347 155 817 734.8 0 817 156 461 734.8 0 461 157 430 734.8 0 430 158 499 734.8 0 499 159 598 734.8 0 598 160 4157 734.8 0 4157 161 4831 734.8 0 4831 162 1541 734.8 0 1541 163 292 734.8 0 292 164 451 734.8 0 451 165 442 734.8 0 442 166 284 734.8 0 284 167 168 734.8 0 168 168 227 734.8 0 227 169 240 734.8 0 240 170 237 734.8 0 237 171 195 734.8 0 195 172 178 734.8 0 178 173 214 734.8 0 214 174 204 734.8 0 204 175 222 734.8 0 222 176 278 734.8 0 278 177 272 734.8 0 272 178 288 734.8 0 288 179 269 734.8 0 269 180 1032 734.8 0 1032 181 1507 734.8 0 1507 182 1049 734.8 0 1049 183 261 734.8 0 261 184 199 734.8 0 199 185 267 734.8 0 267 186 343 734.8 0 343 187 228 734.8 0 228 188 168 734.8 0 168 189 271 734.8 0 271 190 267 734.8 0 267 191 271 734.8 0 271 192 158 734.8 0 158 193 137 734.8 0 137 194 81 734.8 0 81 195 121 734.8 0 121 196 149 734.8 0 149 197 154 734.8 0 154 198 143 734.8 0 143 199 164 734.8 0 164 200 190 734.8 0 190 201 274 734.8 0 274 202 174 734.8 0 174 203 115 734.8 0 115 204 155 734.8 0 155 205 272 734.8 0 272 206 221 734.8 0 221 207 208 734.8 0 208 208 215 734.8 0 215 209 188 734.8 0 188 210 188 734.8 0 188 211 232 734.8 0 232 212 900 734.8 0 900 213 1030 734.8 0 1030 214 372 734.8 0 372 215 239 734.8 0 239 216 355 734.8 0 355 217 329 734.8 0 329 218 283 734.8 0 283 219 176 734.8 0 176 220 242 734.8 0 242 221 248 734.8 0 248 222 284 734.8 0 284 223 217 734.8 0 217 224 284 734.8 0 284 225 396 734.8 0 396 226 320 734.8 0 320 227 260 734.8 0 260 228 210 734.8 0 210 229 259 734.8 0 259 230 233 734.8 0 233 231 222 734.8 0 222 232 168 734.8 0 168 233 187 734.8 0 187 234 207 734.8 0 207 235 156 734.8 0 156 236 178 734.8 0 178 237 240 734.8 0 240 238 268 734.8 0 268 239 236 734.8 0 236 240 263 734.8 0 263 241 405 734.8 0 405 242 292 734.8 0 292 243 321 734.8 0 321 244 293 734.8 0 293 245 349 734.8 0 349 246 309 734.8 0 309 247 325 734.8 0 325 248 226 734.8 0 226 249 160 734.8 0 160 250 267 734.8 0 267 251 235 734.8 0 235 252 163 734.8 0 163 253 176 734.8 0 176 254 455 734.8 0 455 255 495 734.8 0 495 256 889 734.8 0 889 257 613 734.8 0 613 258 264 734.8 0 264 259 163 734.8 0 163 260 207 734.8 0 207 261 189 734.8 0 189 262 201 734.8 0 201 263 167 734.8 0 167 264 230 734.8 0 230 265 238 734.8 0 238 266 662 734.8 0 662 267 487 734.8 0 487 268 354 734.8 0 354 269 235 734.8 0 235 270 298 734.8 0 298 271 418 734.8 0 418 272 823 734.8 0 823 273 2159 734.8 0 2159 274 722 734.8 0 722 275 289 734.8 0 289 276 361 734.8 0 361 277 467 734.8 0 467 278 240 734.8 0 240 279 166 734.8 0 166 280 228 734.8 0 228 281 326 734.8 0 326 282 518 734.8 0 518 283 344 734.8 0 344 284 284 734.8 0 284 285 229 734.8 0 229 286 125 734.8 0 125 287 292 734.8 0 292 288 438 734.8 0 438 289 301 734.8 0 301 290 131 734.8 0 131 291 176 734.8 0 176 292 111 734.8 0 111 293 119 734.8 0 119 294 128 734.8 0 128 295 88 734.8 0 88 296 101 734.8 0 101 297 85 734.8 0 85 298 164 734.8 0 164 299 105 734.8 0 105 300 81 734.8 0 81 301 95 734.8 0 95 Finished in 2.61 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 68,544 Reads with adapters: 6,101 (8.9%) Reads that were too short: 43 (0.1%) Reads written (passing filters): 6,058 (8.8%) Total basepairs processed: 14,261,970 bp Total written (filtered): 1,661,676 bp (11.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6101 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 31 0.0 3 0 30 0 1 17 3701 0.0 3 3583 108 8 2 18 2266 0.0 3 2224 35 4 3 19 12 0.0 3 1 8 3 20 10 0.0 3 0 0 0 10 21 5 0.0 3 0 0 0 5 22 1 0.0 3 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 1 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 6 0.0 3 0 0 0 6 135 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 198 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 7 0.0 3 0 0 0 7 283 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 6,058 Reads with adapters: 518 (8.6%) Reads written (passing filters): 6,058 (100.0%) Total basepairs processed: 1,661,676 bp Total written (filtered): 1,660,117 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 518 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.6% C: 5.4% G: 36.5% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 513 94.7 0 513 4 5 23.7 0 5