This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.32 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 294,299 (9.8%) Reads that were too short: 31,619 (1.1%) Reads written (passing filters): 262,680 (8.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 75,620,127 bp (8.3%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 294299 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1330 734.8 0 1330 7 2197 734.8 0 2197 8 242041 734.8 0 242041 9 4276 734.8 0 4276 10 60 734.8 0 60 11 47 734.8 0 47 12 62 734.8 0 62 13 67 734.8 0 67 14 45 734.8 0 45 15 65 734.8 0 65 16 81 734.8 0 81 17 62 734.8 0 62 18 51 734.8 0 51 19 52 734.8 0 52 20 56 734.8 0 56 21 66 734.8 0 66 22 53 734.8 0 53 23 56 734.8 0 56 24 66 734.8 0 66 25 50 734.8 0 50 26 41 734.8 0 41 27 55 734.8 0 55 28 54 734.8 0 54 29 61 734.8 0 61 30 62 734.8 0 62 31 45 734.8 0 45 32 50 734.8 0 50 33 47 734.8 0 47 34 49 734.8 0 49 35 57 734.8 0 57 36 61 734.8 0 61 37 47 734.8 0 47 38 60 734.8 0 60 39 33 734.8 0 33 40 50 734.8 0 50 41 51 734.8 0 51 42 55 734.8 0 55 43 53 734.8 0 53 44 69 734.8 0 69 45 97 734.8 0 97 46 66 734.8 0 66 47 72 734.8 0 72 48 78 734.8 0 78 49 50 734.8 0 50 50 53 734.8 0 53 51 44 734.8 0 44 52 54 734.8 0 54 53 44 734.8 0 44 54 64 734.8 0 64 55 50 734.8 0 50 56 52 734.8 0 52 57 48 734.8 0 48 58 58 734.8 0 58 59 101 734.8 0 101 60 126 734.8 0 126 61 76 734.8 0 76 62 55 734.8 0 55 63 68 734.8 0 68 64 52 734.8 0 52 65 49 734.8 0 49 66 40 734.8 0 40 67 73 734.8 0 73 68 65 734.8 0 65 69 45 734.8 0 45 70 100 734.8 0 100 71 58 734.8 0 58 72 68 734.8 0 68 73 59 734.8 0 59 74 46 734.8 0 46 75 53 734.8 0 53 76 55 734.8 0 55 77 78 734.8 0 78 78 76 734.8 0 76 79 45 734.8 0 45 80 64 734.8 0 64 81 65 734.8 0 65 82 45 734.8 0 45 83 62 734.8 0 62 84 43 734.8 0 43 85 61 734.8 0 61 86 42 734.8 0 42 87 57 734.8 0 57 88 55 734.8 0 55 89 39 734.8 0 39 90 52 734.8 0 52 91 58 734.8 0 58 92 51 734.8 0 51 93 62 734.8 0 62 94 77 734.8 0 77 95 70 734.8 0 70 96 62 734.8 0 62 97 59 734.8 0 59 98 138 734.8 0 138 99 81 734.8 0 81 100 65 734.8 0 65 101 48 734.8 0 48 102 53 734.8 0 53 103 62 734.8 0 62 104 69 734.8 0 69 105 41 734.8 0 41 106 67 734.8 0 67 107 51 734.8 0 51 108 46 734.8 0 46 109 50 734.8 0 50 110 59 734.8 0 59 111 57 734.8 0 57 112 63 734.8 0 63 113 91 734.8 0 91 114 68 734.8 0 68 115 62 734.8 0 62 116 47 734.8 0 47 117 64 734.8 0 64 118 64 734.8 0 64 119 63 734.8 0 63 120 61 734.8 0 61 121 57 734.8 0 57 122 47 734.8 0 47 123 55 734.8 0 55 124 65 734.8 0 65 125 60 734.8 0 60 126 60 734.8 0 60 127 69 734.8 0 69 128 88 734.8 0 88 129 67 734.8 0 67 130 100 734.8 0 100 131 89 734.8 0 89 132 102 734.8 0 102 133 82 734.8 0 82 134 90 734.8 0 90 135 89 734.8 0 89 136 61 734.8 0 61 137 62 734.8 0 62 138 106 734.8 0 106 139 82 734.8 0 82 140 100 734.8 0 100 141 71 734.8 0 71 142 70 734.8 0 70 143 89 734.8 0 89 144 95 734.8 0 95 145 181 734.8 0 181 146 63 734.8 0 63 147 57 734.8 0 57 148 63 734.8 0 63 149 54 734.8 0 54 150 52 734.8 0 52 151 68 734.8 0 68 152 69 734.8 0 69 153 56 734.8 0 56 154 42 734.8 0 42 155 68 734.8 0 68 156 83 734.8 0 83 157 113 734.8 0 113 158 78 734.8 0 78 159 72 734.8 0 72 160 74 734.8 0 74 161 53 734.8 0 53 162 49 734.8 0 49 163 37 734.8 0 37 164 67 734.8 0 67 165 53 734.8 0 53 166 47 734.8 0 47 167 59 734.8 0 59 168 54 734.8 0 54 169 48 734.8 0 48 170 47 734.8 0 47 171 73 734.8 0 73 172 42 734.8 0 42 173 54 734.8 0 54 174 55 734.8 0 55 175 59 734.8 0 59 176 52 734.8 0 52 177 56 734.8 0 56 178 51 734.8 0 51 179 56 734.8 0 56 180 62 734.8 0 62 181 59 734.8 0 59 182 53 734.8 0 53 183 54 734.8 0 54 184 67 734.8 0 67 185 66 734.8 0 66 186 56 734.8 0 56 187 76 734.8 0 76 188 49 734.8 0 49 189 54 734.8 0 54 190 76 734.8 0 76 191 241 734.8 0 241 192 187 734.8 0 187 193 92 734.8 0 92 194 69 734.8 0 69 195 42 734.8 0 42 196 90 734.8 0 90 197 258 734.8 0 258 198 240 734.8 0 240 199 92 734.8 0 92 200 63 734.8 0 63 201 78 734.8 0 78 202 39 734.8 0 39 203 42 734.8 0 42 204 64 734.8 0 64 205 93 734.8 0 93 206 58 734.8 0 58 207 58 734.8 0 58 208 51 734.8 0 51 209 60 734.8 0 60 210 57 734.8 0 57 211 60 734.8 0 60 212 62 734.8 0 62 213 64 734.8 0 64 214 60 734.8 0 60 215 53 734.8 0 53 216 51 734.8 0 51 217 51 734.8 0 51 218 78 734.8 0 78 219 83 734.8 0 83 220 59 734.8 0 59 221 89 734.8 0 89 222 71 734.8 0 71 223 100 734.8 0 100 224 368 734.8 0 368 225 76 734.8 0 76 226 65 734.8 0 65 227 45 734.8 0 45 228 54 734.8 0 54 229 63 734.8 0 63 230 68 734.8 0 68 231 76 734.8 0 76 232 107 734.8 0 107 233 96 734.8 0 96 234 94 734.8 0 94 235 56 734.8 0 56 236 75 734.8 0 75 237 85 734.8 0 85 238 87 734.8 0 87 239 93 734.8 0 93 240 98 734.8 0 98 241 77 734.8 0 77 242 62 734.8 0 62 243 54 734.8 0 54 244 65 734.8 0 65 245 95 734.8 0 95 246 84 734.8 0 84 247 53 734.8 0 53 248 52 734.8 0 52 249 53 734.8 0 53 250 58 734.8 0 58 251 46 734.8 0 46 252 41 734.8 0 41 253 62 734.8 0 62 254 65 734.8 0 65 255 86 734.8 0 86 256 134 734.8 0 134 257 144 734.8 0 144 258 109 734.8 0 109 259 82 734.8 0 82 260 86 734.8 0 86 261 88 734.8 0 88 262 79 734.8 0 79 263 76 734.8 0 76 264 99 734.8 0 99 265 208 734.8 0 208 266 290 734.8 0 290 267 118 734.8 0 118 268 189 734.8 0 189 269 54 734.8 0 54 270 47 734.8 0 47 271 59 734.8 0 59 272 72 734.8 0 72 273 109 734.8 0 109 274 78 734.8 0 78 275 51 734.8 0 51 276 59 734.8 0 59 277 69 734.8 0 69 278 70 734.8 0 70 279 53 734.8 0 53 280 109 734.8 0 109 281 165 734.8 0 165 282 120 734.8 0 120 283 126 734.8 0 126 284 164 734.8 0 164 285 222 734.8 0 222 286 178 734.8 0 178 287 142 734.8 0 142 288 251 734.8 0 251 289 246 734.8 0 246 290 323 734.8 0 323 291 480 734.8 0 480 292 545 734.8 0 545 293 643 734.8 0 643 294 518 734.8 0 518 295 610 734.8 0 610 296 637 734.8 0 637 297 779 734.8 0 779 298 4809 734.8 0 4809 299 4477 734.8 0 4477 300 8365 734.8 0 8365 301 1325 734.8 0 1325 Finished in 13.41 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 262,680 Reads with adapters: 6,662 (2.5%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 6,645 (2.5%) Total basepairs processed: 75,620,127 bp Total written (filtered): 1,535,934 bp (2.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6662 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 4 0.0 3 0 0 4 19 74 0.0 3 0 74 20 3981 0.0 4 3926 53 1 1 21 20 0.0 4 11 5 0 3 1 22 1 0.0 4 0 0 0 1 23 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 125 6 0.0 4 0 0 0 0 6 126 21 0.0 4 0 0 0 0 21 127 2522 0.0 4 0 0 0 0 2522 128 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 152 5 0.0 4 0 0 0 0 5 153 4 0.0 4 0 0 0 0 4 208 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 275 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 6,645 Reads with adapters: 91 (1.4%) Reads written (passing filters): 6,645 (100.0%) Total basepairs processed: 1,535,934 bp Total written (filtered): 1,534,526 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 91 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 0.0% G: 97.8% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 16 0.0 0 0 16 10 55 0.0 1 1 54 11 18 0.0 1 0 18 257 1 0.0 1 1 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.