This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCTCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.24 s (25 us/read; 2.43 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 23,197 (0.8%) Reads that were too short: 5,569 (0.2%) Reads written (passing filters): 17,628 (0.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 3,511,175 bp (0.4%) === Adapter 1 === Sequence: TTCTCG; Type: regular 5'; Length: 6; Trimmed: 23197 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 95 734.8 0 95 7 47 734.8 0 47 8 65 734.8 0 65 9 92 734.8 0 92 10 40 734.8 0 40 11 47 734.8 0 47 12 55 734.8 0 55 13 44 734.8 0 44 14 42 734.8 0 42 15 36 734.8 0 36 16 38 734.8 0 38 17 59 734.8 0 59 18 40 734.8 0 40 19 43 734.8 0 43 20 37 734.8 0 37 21 48 734.8 0 48 22 38 734.8 0 38 23 48 734.8 0 48 24 54 734.8 0 54 25 53 734.8 0 53 26 43 734.8 0 43 27 65 734.8 0 65 28 36 734.8 0 36 29 46 734.8 0 46 30 42 734.8 0 42 31 39 734.8 0 39 32 48 734.8 0 48 33 48 734.8 0 48 34 49 734.8 0 49 35 45 734.8 0 45 36 46 734.8 0 46 37 80 734.8 0 80 38 59 734.8 0 59 39 781 734.8 0 781 40 325 734.8 0 325 41 32 734.8 0 32 42 30 734.8 0 30 43 31 734.8 0 31 44 43 734.8 0 43 45 31 734.8 0 31 46 36 734.8 0 36 47 37 734.8 0 37 48 47 734.8 0 47 49 40 734.8 0 40 50 40 734.8 0 40 51 42 734.8 0 42 52 82 734.8 0 82 53 65 734.8 0 65 54 54 734.8 0 54 55 29 734.8 0 29 56 74 734.8 0 74 57 34 734.8 0 34 58 68 734.8 0 68 59 42 734.8 0 42 60 33 734.8 0 33 61 34 734.8 0 34 62 45 734.8 0 45 63 40 734.8 0 40 64 38 734.8 0 38 65 38 734.8 0 38 66 38 734.8 0 38 67 52 734.8 0 52 68 43 734.8 0 43 69 38 734.8 0 38 70 36 734.8 0 36 71 42 734.8 0 42 72 41 734.8 0 41 73 50 734.8 0 50 74 44 734.8 0 44 75 67 734.8 0 67 76 45 734.8 0 45 77 84 734.8 0 84 78 53 734.8 0 53 79 49 734.8 0 49 80 53 734.8 0 53 81 49 734.8 0 49 82 67 734.8 0 67 83 70 734.8 0 70 84 56 734.8 0 56 85 71 734.8 0 71 86 65 734.8 0 65 87 67 734.8 0 67 88 69 734.8 0 69 89 114 734.8 0 114 90 1953 734.8 0 1953 91 1241 734.8 0 1241 92 412 734.8 0 412 93 57 734.8 0 57 94 80 734.8 0 80 95 54 734.8 0 54 96 65 734.8 0 65 97 73 734.8 0 73 98 60 734.8 0 60 99 49 734.8 0 49 100 50 734.8 0 50 101 52 734.8 0 52 102 37 734.8 0 37 103 52 734.8 0 52 104 44 734.8 0 44 105 55 734.8 0 55 106 123 734.8 0 123 107 81 734.8 0 81 108 152 734.8 0 152 109 60 734.8 0 60 110 100 734.8 0 100 111 1235 734.8 0 1235 112 877 734.8 0 877 113 125 734.8 0 125 114 53 734.8 0 53 115 73 734.8 0 73 116 50 734.8 0 50 117 67 734.8 0 67 118 60 734.8 0 60 119 39 734.8 0 39 120 40 734.8 0 40 121 46 734.8 0 46 122 35 734.8 0 35 123 36 734.8 0 36 124 43 734.8 0 43 125 33 734.8 0 33 126 47 734.8 0 47 127 35 734.8 0 35 128 42 734.8 0 42 129 46 734.8 0 46 130 63 734.8 0 63 131 57 734.8 0 57 132 63 734.8 0 63 133 52 734.8 0 52 134 38 734.8 0 38 135 56 734.8 0 56 136 41 734.8 0 41 137 50 734.8 0 50 138 43 734.8 0 43 139 40 734.8 0 40 140 56 734.8 0 56 141 52 734.8 0 52 142 49 734.8 0 49 143 147 734.8 0 147 144 101 734.8 0 101 145 67 734.8 0 67 146 44 734.8 0 44 147 47 734.8 0 47 148 43 734.8 0 43 149 58 734.8 0 58 150 47 734.8 0 47 151 50 734.8 0 50 152 62 734.8 0 62 153 57 734.8 0 57 154 39 734.8 0 39 155 57 734.8 0 57 156 53 734.8 0 53 157 41 734.8 0 41 158 42 734.8 0 42 159 79 734.8 0 79 160 73 734.8 0 73 161 196 734.8 0 196 162 128 734.8 0 128 163 128 734.8 0 128 164 214 734.8 0 214 165 58 734.8 0 58 166 50 734.8 0 50 167 27 734.8 0 27 168 49 734.8 0 49 169 70 734.8 0 70 170 56 734.8 0 56 171 64 734.8 0 64 172 77 734.8 0 77 173 41 734.8 0 41 174 38 734.8 0 38 175 34 734.8 0 34 176 41 734.8 0 41 177 172 734.8 0 172 178 118 734.8 0 118 179 55 734.8 0 55 180 42 734.8 0 42 181 49 734.8 0 49 182 51 734.8 0 51 183 40 734.8 0 40 184 36 734.8 0 36 185 57 734.8 0 57 186 43 734.8 0 43 187 43 734.8 0 43 188 65 734.8 0 65 189 78 734.8 0 78 190 81 734.8 0 81 191 72 734.8 0 72 192 48 734.8 0 48 193 48 734.8 0 48 194 47 734.8 0 47 195 43 734.8 0 43 196 71 734.8 0 71 197 87 734.8 0 87 198 36 734.8 0 36 199 59 734.8 0 59 200 50 734.8 0 50 201 46 734.8 0 46 202 32 734.8 0 32 203 53 734.8 0 53 204 54 734.8 0 54 205 45 734.8 0 45 206 45 734.8 0 45 207 47 734.8 0 47 208 35 734.8 0 35 209 50 734.8 0 50 210 47 734.8 0 47 211 87 734.8 0 87 212 87 734.8 0 87 213 87 734.8 0 87 214 67 734.8 0 67 215 93 734.8 0 93 216 77 734.8 0 77 217 69 734.8 0 69 218 46 734.8 0 46 219 39 734.8 0 39 220 38 734.8 0 38 221 32 734.8 0 32 222 47 734.8 0 47 223 59 734.8 0 59 224 109 734.8 0 109 225 184 734.8 0 184 226 83 734.8 0 83 227 45 734.8 0 45 228 41 734.8 0 41 229 41 734.8 0 41 230 53 734.8 0 53 231 49 734.8 0 49 232 46 734.8 0 46 233 58 734.8 0 58 234 73 734.8 0 73 235 55 734.8 0 55 236 94 734.8 0 94 237 54 734.8 0 54 238 58 734.8 0 58 239 47 734.8 0 47 240 34 734.8 0 34 241 37 734.8 0 37 242 118 734.8 0 118 243 188 734.8 0 188 244 294 734.8 0 294 245 229 734.8 0 229 246 181 734.8 0 181 247 92 734.8 0 92 248 31 734.8 0 31 249 27 734.8 0 27 250 35 734.8 0 35 251 22 734.8 0 22 252 35 734.8 0 35 253 48 734.8 0 48 254 53 734.8 0 53 255 53 734.8 0 53 256 53 734.8 0 53 257 29 734.8 0 29 258 36 734.8 0 36 259 34 734.8 0 34 260 31 734.8 0 31 261 35 734.8 0 35 262 38 734.8 0 38 263 68 734.8 0 68 264 78 734.8 0 78 265 73 734.8 0 73 266 43 734.8 0 43 267 29 734.8 0 29 268 29 734.8 0 29 269 39 734.8 0 39 270 25 734.8 0 25 271 34 734.8 0 34 272 27 734.8 0 27 273 26 734.8 0 26 274 21 734.8 0 21 275 33 734.8 0 33 276 33 734.8 0 33 277 43 734.8 0 43 278 43 734.8 0 43 279 31 734.8 0 31 280 22 734.8 0 22 281 37 734.8 0 37 282 31 734.8 0 31 283 37 734.8 0 37 284 48 734.8 0 48 285 48 734.8 0 48 286 57 734.8 0 57 287 38 734.8 0 38 288 33 734.8 0 33 289 26 734.8 0 26 290 34 734.8 0 34 291 39 734.8 0 39 292 22 734.8 0 22 293 35 734.8 0 35 294 31 734.8 0 31 295 12 734.8 0 12 296 61 734.8 0 61 297 111 734.8 0 111 298 58 734.8 0 58 299 18 734.8 0 18 300 28 734.8 0 28 301 9 734.8 0 9 Finished in 0.62 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 17,628 Reads with adapters: 57 (0.3%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 53 (0.3%) Total basepairs processed: 3,511,175 bp Total written (filtered): 6,424 bp (0.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 57 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 0 1 19 1 0.0 3 1 43 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 101 28 0.0 3 0 0 0 28 102 3 0.0 3 0 0 0 3 104 12 0.0 3 0 0 0 12 105 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 202 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (189 us/read; 0.32 M reads/minute). === Summary === Total reads processed: 53 Reads with adapters: 3 (5.7%) Reads written (passing filters): 53 (100.0%) Total basepairs processed: 6,424 bp Total written (filtered): 6,414 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 66.7% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 0.8 0 2 4 1 0.2 0 1