This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 80.74 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 34,535 (1.1%) Reads that were too short: 10,293 (0.3%) Reads written (passing filters): 24,242 (0.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 5,859,812 bp (0.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 34535 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 387 734.8 0 387 7 1836 734.8 0 1836 8 10806 734.8 0 10806 9 56 734.8 0 56 10 11 734.8 0 11 11 23 734.8 0 23 12 16 734.8 0 16 13 27 734.8 0 27 14 23 734.8 0 23 15 22 734.8 0 22 16 24 734.8 0 24 17 21 734.8 0 21 18 38 734.8 0 38 19 22 734.8 0 22 20 27 734.8 0 27 21 21 734.8 0 21 22 24 734.8 0 24 23 34 734.8 0 34 24 22 734.8 0 22 25 23 734.8 0 23 26 34 734.8 0 34 27 20 734.8 0 20 28 38 734.8 0 38 29 26 734.8 0 26 30 49 734.8 0 49 31 228 734.8 0 228 32 572 734.8 0 572 33 41 734.8 0 41 34 23 734.8 0 23 35 29 734.8 0 29 36 33 734.8 0 33 37 40 734.8 0 40 38 21 734.8 0 21 39 20 734.8 0 20 40 38 734.8 0 38 41 27 734.8 0 27 42 17 734.8 0 17 43 54 734.8 0 54 44 36 734.8 0 36 45 21 734.8 0 21 46 30 734.8 0 30 47 36 734.8 0 36 48 22 734.8 0 22 49 28 734.8 0 28 50 13 734.8 0 13 51 21 734.8 0 21 52 28 734.8 0 28 53 32 734.8 0 32 54 38 734.8 0 38 55 32 734.8 0 32 56 12 734.8 0 12 57 14 734.8 0 14 58 29 734.8 0 29 59 32 734.8 0 32 60 25 734.8 0 25 61 25 734.8 0 25 62 35 734.8 0 35 63 27 734.8 0 27 64 140 734.8 0 140 65 378 734.8 0 378 66 70 734.8 0 70 67 20 734.8 0 20 68 33 734.8 0 33 69 71 734.8 0 71 70 30 734.8 0 30 71 25 734.8 0 25 72 12 734.8 0 12 73 11 734.8 0 11 74 13 734.8 0 13 75 20 734.8 0 20 76 20 734.8 0 20 77 21 734.8 0 21 78 16 734.8 0 16 79 17 734.8 0 17 80 27 734.8 0 27 81 46 734.8 0 46 82 25 734.8 0 25 83 47 734.8 0 47 84 44 734.8 0 44 85 34 734.8 0 34 86 24 734.8 0 24 87 18 734.8 0 18 88 31 734.8 0 31 89 27 734.8 0 27 90 33 734.8 0 33 91 29 734.8 0 29 92 209 734.8 0 209 93 144 734.8 0 144 94 55 734.8 0 55 95 80 734.8 0 80 96 44 734.8 0 44 97 27 734.8 0 27 98 25 734.8 0 25 99 32 734.8 0 32 100 55 734.8 0 55 101 52 734.8 0 52 102 35 734.8 0 35 103 31 734.8 0 31 104 32 734.8 0 32 105 28 734.8 0 28 106 25 734.8 0 25 107 23 734.8 0 23 108 19 734.8 0 19 109 30 734.8 0 30 110 38 734.8 0 38 111 38 734.8 0 38 112 22 734.8 0 22 113 29 734.8 0 29 114 37 734.8 0 37 115 43 734.8 0 43 116 42 734.8 0 42 117 74 734.8 0 74 118 47 734.8 0 47 119 30 734.8 0 30 120 41 734.8 0 41 121 46 734.8 0 46 122 57 734.8 0 57 123 62 734.8 0 62 124 41 734.8 0 41 125 49 734.8 0 49 126 36 734.8 0 36 127 45 734.8 0 45 128 48 734.8 0 48 129 46 734.8 0 46 130 69 734.8 0 69 131 71 734.8 0 71 132 68 734.8 0 68 133 39 734.8 0 39 134 48 734.8 0 48 135 59 734.8 0 59 136 89 734.8 0 89 137 95 734.8 0 95 138 66 734.8 0 66 139 49 734.8 0 49 140 49 734.8 0 49 141 58 734.8 0 58 142 44 734.8 0 44 143 65 734.8 0 65 144 88 734.8 0 88 145 51 734.8 0 51 146 50 734.8 0 50 147 144 734.8 0 144 148 111 734.8 0 111 149 97 734.8 0 97 150 97 734.8 0 97 151 59 734.8 0 59 152 65 734.8 0 65 153 124 734.8 0 124 154 50 734.8 0 50 155 84 734.8 0 84 156 126 734.8 0 126 157 135 734.8 0 135 158 119 734.8 0 119 159 97 734.8 0 97 160 99 734.8 0 99 161 95 734.8 0 95 162 84 734.8 0 84 163 61 734.8 0 61 164 60 734.8 0 60 165 42 734.8 0 42 166 66 734.8 0 66 167 114 734.8 0 114 168 131 734.8 0 131 169 77 734.8 0 77 170 76 734.8 0 76 171 92 734.8 0 92 172 84 734.8 0 84 173 86 734.8 0 86 174 52 734.8 0 52 175 138 734.8 0 138 176 299 734.8 0 299 177 147 734.8 0 147 178 71 734.8 0 71 179 69 734.8 0 69 180 37 734.8 0 37 181 63 734.8 0 63 182 69 734.8 0 69 183 77 734.8 0 77 184 89 734.8 0 89 185 58 734.8 0 58 186 108 734.8 0 108 187 108 734.8 0 108 188 162 734.8 0 162 189 96 734.8 0 96 190 65 734.8 0 65 191 56 734.8 0 56 192 44 734.8 0 44 193 56 734.8 0 56 194 54 734.8 0 54 195 62 734.8 0 62 196 53 734.8 0 53 197 57 734.8 0 57 198 35 734.8 0 35 199 71 734.8 0 71 200 64 734.8 0 64 201 41 734.8 0 41 202 43 734.8 0 43 203 49 734.8 0 49 204 65 734.8 0 65 205 57 734.8 0 57 206 62 734.8 0 62 207 52 734.8 0 52 208 60 734.8 0 60 209 53 734.8 0 53 210 70 734.8 0 70 211 57 734.8 0 57 212 84 734.8 0 84 213 73 734.8 0 73 214 109 734.8 0 109 215 116 734.8 0 116 216 132 734.8 0 132 217 108 734.8 0 108 218 89 734.8 0 89 219 91 734.8 0 91 220 85 734.8 0 85 221 127 734.8 0 127 222 143 734.8 0 143 223 214 734.8 0 214 224 105 734.8 0 105 225 124 734.8 0 124 226 93 734.8 0 93 227 135 734.8 0 135 228 97 734.8 0 97 229 105 734.8 0 105 230 76 734.8 0 76 231 62 734.8 0 62 232 66 734.8 0 66 233 105 734.8 0 105 234 63 734.8 0 63 235 93 734.8 0 93 236 99 734.8 0 99 237 92 734.8 0 92 238 116 734.8 0 116 239 106 734.8 0 106 240 85 734.8 0 85 241 147 734.8 0 147 242 123 734.8 0 123 243 182 734.8 0 182 244 107 734.8 0 107 245 123 734.8 0 123 246 75 734.8 0 75 247 100 734.8 0 100 248 162 734.8 0 162 249 144 734.8 0 144 250 49 734.8 0 49 251 44 734.8 0 44 252 59 734.8 0 59 253 105 734.8 0 105 254 166 734.8 0 166 255 160 734.8 0 160 256 131 734.8 0 131 257 97 734.8 0 97 258 82 734.8 0 82 259 77 734.8 0 77 260 88 734.8 0 88 261 52 734.8 0 52 262 109 734.8 0 109 263 124 734.8 0 124 264 144 734.8 0 144 265 136 734.8 0 136 266 100 734.8 0 100 267 104 734.8 0 104 268 244 734.8 0 244 269 207 734.8 0 207 270 81 734.8 0 81 271 66 734.8 0 66 272 87 734.8 0 87 273 127 734.8 0 127 274 145 734.8 0 145 275 126 734.8 0 126 276 95 734.8 0 95 277 90 734.8 0 90 278 85 734.8 0 85 279 84 734.8 0 84 280 154 734.8 0 154 281 99 734.8 0 99 282 88 734.8 0 88 283 113 734.8 0 113 284 94 734.8 0 94 285 144 734.8 0 144 286 100 734.8 0 100 287 205 734.8 0 205 288 156 734.8 0 156 289 60 734.8 0 60 290 62 734.8 0 62 291 57 734.8 0 57 292 122 734.8 0 122 293 185 734.8 0 185 294 123 734.8 0 123 295 75 734.8 0 75 296 50 734.8 0 50 297 35 734.8 0 35 298 71 734.8 0 71 299 90 734.8 0 90 300 152 734.8 0 152 301 70 734.8 0 70 Finished in 1.12 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 24,242 Reads with adapters: 11,017 (45.4%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 11,013 (45.4%) Total basepairs processed: 5,859,812 bp Total written (filtered): 3,001,314 bp (51.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11017 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 10 19 104 0.0 3 0 101 1 1 1 20 10698 0.0 4 10572 123 1 0 2 21 30 0.0 4 8 21 0 0 1 22 8 0.0 4 4 1 3 23 102 0.0 4 95 0 0 7 24 1 0.0 4 0 1 29 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 108 15 0.0 4 0 0 0 15 110 1 0.0 4 0 0 0 0 1 111 42 0.0 4 0 0 0 40 2 130 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 11,013 Reads with adapters: 2,829 (25.7%) Reads written (passing filters): 11,013 (100.0%) Total basepairs processed: 3,001,314 bp Total written (filtered): 2,356,717 bp (78.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2829 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 85.8% C: 1.7% G: 12.4% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 5 2 10.8 0 2 10 74 0.0 1 1 73 11 276 0.0 1 0 276 13 1 0.0 1 0 1 170 1 0.0 1 1 230 14 0.0 1 13 1 232 1 0.0 1 1 234 1 0.0 1 1 255 1 0.0 1 1 257 2 0.0 1 2 258 40 0.0 1 39 1 259 2346 0.0 1 2255 91 260 40 0.0 1 37 3 261 27 0.0 1 26 1 262 2 0.0 1 2 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.