This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCTAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.49 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 244,317 (8.1%) Reads that were too short: 76,914 (2.6%) Reads written (passing filters): 167,403 (5.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 40,636,030 bp (4.5%) === Adapter 1 === Sequence: GGCTAC; Type: regular 5'; Length: 6; Trimmed: 244317 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 666 734.8 0 666 7 96349 734.8 0 96349 8 120 734.8 0 120 9 26 734.8 0 26 10 18 734.8 0 18 11 30 734.8 0 30 12 36 734.8 0 36 13 211 734.8 0 211 14 471 734.8 0 471 15 173 734.8 0 173 16 39 734.8 0 39 17 17 734.8 0 17 18 24 734.8 0 24 19 17 734.8 0 17 20 16 734.8 0 16 21 13 734.8 0 13 22 28 734.8 0 28 23 37 734.8 0 37 24 17 734.8 0 17 25 12 734.8 0 12 26 13 734.8 0 13 27 23 734.8 0 23 28 29 734.8 0 29 29 585 734.8 0 585 30 26 734.8 0 26 31 22 734.8 0 22 32 27 734.8 0 27 33 13 734.8 0 13 34 18 734.8 0 18 35 20 734.8 0 20 36 14 734.8 0 14 37 14 734.8 0 14 38 17 734.8 0 17 39 28 734.8 0 28 40 50 734.8 0 50 41 29 734.8 0 29 42 20 734.8 0 20 43 20 734.8 0 20 44 17 734.8 0 17 45 14 734.8 0 14 46 12 734.8 0 12 47 14 734.8 0 14 48 17 734.8 0 17 49 28 734.8 0 28 50 51 734.8 0 51 51 32 734.8 0 32 52 20 734.8 0 20 53 20 734.8 0 20 54 17 734.8 0 17 55 15 734.8 0 15 56 20 734.8 0 20 57 13 734.8 0 13 58 21 734.8 0 21 59 11 734.8 0 11 60 10 734.8 0 10 61 13 734.8 0 13 62 21 734.8 0 21 63 24 734.8 0 24 64 13 734.8 0 13 65 14 734.8 0 14 66 15 734.8 0 15 67 11 734.8 0 11 68 16 734.8 0 16 69 20 734.8 0 20 70 15 734.8 0 15 71 17 734.8 0 17 72 12 734.8 0 12 73 6 734.8 0 6 74 11 734.8 0 11 75 14 734.8 0 14 76 20 734.8 0 20 77 18 734.8 0 18 78 12 734.8 0 12 79 20 734.8 0 20 80 20 734.8 0 20 81 31 734.8 0 31 82 25 734.8 0 25 83 84 734.8 0 84 84 119 734.8 0 119 85 35 734.8 0 35 86 25 734.8 0 25 87 77 734.8 0 77 88 77 734.8 0 77 89 70 734.8 0 70 90 48 734.8 0 48 91 26 734.8 0 26 92 17 734.8 0 17 93 21 734.8 0 21 94 28 734.8 0 28 95 25 734.8 0 25 96 26 734.8 0 26 97 21 734.8 0 21 98 16 734.8 0 16 99 22 734.8 0 22 100 31 734.8 0 31 101 29 734.8 0 29 102 37 734.8 0 37 103 22 734.8 0 22 104 24 734.8 0 24 105 16 734.8 0 16 106 31 734.8 0 31 107 26 734.8 0 26 108 34 734.8 0 34 109 70 734.8 0 70 110 126 734.8 0 126 111 313 734.8 0 313 112 70 734.8 0 70 113 133 734.8 0 133 114 156 734.8 0 156 115 96 734.8 0 96 116 152 734.8 0 152 117 200 734.8 0 200 118 148 734.8 0 148 119 287 734.8 0 287 120 261 734.8 0 261 121 365 734.8 0 365 122 451 734.8 0 451 123 875 734.8 0 875 124 18380 734.8 0 18380 125 26106 734.8 0 26106 126 3172 734.8 0 3172 127 151 734.8 0 151 128 40 734.8 0 40 129 55 734.8 0 55 130 76 734.8 0 76 131 40 734.8 0 40 132 31 734.8 0 31 133 67 734.8 0 67 134 38 734.8 0 38 135 27 734.8 0 27 136 30 734.8 0 30 137 29 734.8 0 29 138 19 734.8 0 19 139 28 734.8 0 28 140 25 734.8 0 25 141 41 734.8 0 41 142 33 734.8 0 33 143 44 734.8 0 44 144 41 734.8 0 41 145 75 734.8 0 75 146 83 734.8 0 83 147 151 734.8 0 151 148 95 734.8 0 95 149 70 734.8 0 70 150 37 734.8 0 37 151 79 734.8 0 79 152 404 734.8 0 404 153 726 734.8 0 726 154 165 734.8 0 165 155 85 734.8 0 85 156 127 734.8 0 127 157 783 734.8 0 783 158 929 734.8 0 929 159 948 734.8 0 948 160 797 734.8 0 797 161 460 734.8 0 460 162 259 734.8 0 259 163 50 734.8 0 50 164 51 734.8 0 51 165 18 734.8 0 18 166 43 734.8 0 43 167 45 734.8 0 45 168 63 734.8 0 63 169 51 734.8 0 51 170 71 734.8 0 71 171 51 734.8 0 51 172 126 734.8 0 126 173 182 734.8 0 182 174 117 734.8 0 117 175 81 734.8 0 81 176 193 734.8 0 193 177 2936 734.8 0 2936 178 2482 734.8 0 2482 179 756 734.8 0 756 180 79 734.8 0 79 181 25 734.8 0 25 182 39 734.8 0 39 183 34 734.8 0 34 184 25 734.8 0 25 185 17 734.8 0 17 186 26 734.8 0 26 187 22 734.8 0 22 188 17 734.8 0 17 189 19 734.8 0 19 190 47 734.8 0 47 191 37 734.8 0 37 192 38 734.8 0 38 193 32 734.8 0 32 194 44 734.8 0 44 195 24 734.8 0 24 196 37 734.8 0 37 197 23 734.8 0 23 198 16 734.8 0 16 199 19 734.8 0 19 200 18 734.8 0 18 201 25 734.8 0 25 202 17 734.8 0 17 203 20 734.8 0 20 204 19 734.8 0 19 205 20 734.8 0 20 206 18 734.8 0 18 207 46 734.8 0 46 208 113 734.8 0 113 209 68 734.8 0 68 210 32 734.8 0 32 211 19 734.8 0 19 212 16 734.8 0 16 213 46 734.8 0 46 214 35 734.8 0 35 215 32 734.8 0 32 216 34 734.8 0 34 217 22 734.8 0 22 218 28 734.8 0 28 219 34 734.8 0 34 220 55 734.8 0 55 221 28 734.8 0 28 222 27 734.8 0 27 223 27 734.8 0 27 224 25 734.8 0 25 225 21 734.8 0 21 226 22 734.8 0 22 227 18 734.8 0 18 228 21 734.8 0 21 229 35 734.8 0 35 230 42 734.8 0 42 231 31 734.8 0 31 232 31 734.8 0 31 233 77 734.8 0 77 234 178 734.8 0 178 235 100 734.8 0 100 236 51 734.8 0 51 237 15 734.8 0 15 238 22 734.8 0 22 239 32 734.8 0 32 240 44 734.8 0 44 241 23 734.8 0 23 242 23 734.8 0 23 243 24 734.8 0 24 244 25 734.8 0 25 245 21 734.8 0 21 246 28 734.8 0 28 247 40 734.8 0 40 248 24 734.8 0 24 249 49 734.8 0 49 250 23 734.8 0 23 251 20 734.8 0 20 252 35 734.8 0 35 253 35 734.8 0 35 254 36 734.8 0 36 255 34 734.8 0 34 256 69 734.8 0 69 257 458 734.8 0 458 258 1269 734.8 0 1269 259 210 734.8 0 210 260 112 734.8 0 112 261 159 734.8 0 159 262 49 734.8 0 49 263 312 734.8 0 312 264 495 734.8 0 495 265 116 734.8 0 116 266 30 734.8 0 30 267 77 734.8 0 77 268 292 734.8 0 292 269 766 734.8 0 766 270 130 734.8 0 130 271 41 734.8 0 41 272 25 734.8 0 25 273 35 734.8 0 35 274 35 734.8 0 35 275 61 734.8 0 61 276 36 734.8 0 36 277 21 734.8 0 21 278 19 734.8 0 19 279 44 734.8 0 44 280 107 734.8 0 107 281 126 734.8 0 126 282 45 734.8 0 45 283 63 734.8 0 63 284 59 734.8 0 59 285 45 734.8 0 45 286 78 734.8 0 78 287 141 734.8 0 141 288 1126 734.8 0 1126 289 1785 734.8 0 1785 290 563 734.8 0 563 291 254 734.8 0 254 292 1076 734.8 0 1076 293 20778 734.8 0 20778 294 31876 734.8 0 31876 295 10841 734.8 0 10841 296 782 734.8 0 782 297 128 734.8 0 128 298 129 734.8 0 129 299 43 734.8 0 43 300 26 734.8 0 26 301 21 734.8 0 21 Finished in 6.73 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 167,403 Reads with adapters: 96,662 (57.7%) Reads that were too short: 72 (0.0%) Reads written (passing filters): 96,590 (57.7%) Total basepairs processed: 40,636,030 bp Total written (filtered): 26,747,148 bp (65.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 96662 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 36 0.0 3 0 0 32 4 16 741 0.0 3 0 663 47 31 17 95389 0.0 3 92496 2469 321 103 18 176 0.0 3 12 153 10 1 19 87 0.0 3 0 0 54 33 20 98 0.0 3 0 0 1 97 22 1 0.0 3 0 0 0 1 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 1 35 1 0.0 3 1 37 1 0.0 3 0 0 0 1 38 3 0.0 3 0 0 0 3 39 1 0.0 3 1 40 14 0.0 3 0 0 0 14 41 2 0.0 3 0 0 0 2 65 4 0.0 3 0 0 0 4 68 19 0.0 3 0 0 1 18 71 7 0.0 3 0 0 0 7 86 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 116 6 0.0 3 0 0 0 6 117 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 126 20 0.0 3 0 0 0 20 127 5 0.0 3 0 0 1 4 129 19 0.0 3 0 0 3 16 130 3 0.0 3 0 0 1 2 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.46 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 96,590 Reads with adapters: 10,360 (10.7%) Reads written (passing filters): 96,590 (100.0%) Total basepairs processed: 26,747,148 bp Total written (filtered): 26,715,990 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 10360 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.5% C: 71.6% G: 3.0% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 10304 1509.2 0 10304 4 46 377.3 0 46 5 7 94.3 0 7 7 1 5.9 0 1 8 1 1.5 0 1 12 1 0.0 1 0 1