This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 82.14 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 457,920 (15.2%) Reads that were too short: 5,987 (0.2%) Reads written (passing filters): 451,933 (15.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 114,505,930 bp (12.6%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 457920 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1079 734.8 0 1079 7 7272 734.8 0 7272 8 32 734.8 0 32 9 198 734.8 0 198 10 25 734.8 0 25 11 43 734.8 0 43 12 275 734.8 0 275 13 12 734.8 0 12 14 10 734.8 0 10 15 22 734.8 0 22 16 17 734.8 0 17 17 42 734.8 0 42 18 19 734.8 0 19 19 52 734.8 0 52 20 53 734.8 0 53 21 151 734.8 0 151 22 548 734.8 0 548 23 81 734.8 0 81 24 233 734.8 0 233 25 200 734.8 0 200 26 149 734.8 0 149 27 511 734.8 0 511 28 159 734.8 0 159 29 278 734.8 0 278 30 309 734.8 0 309 31 171 734.8 0 171 32 375 734.8 0 375 33 520 734.8 0 520 34 874 734.8 0 874 35 1366 734.8 0 1366 36 2282 734.8 0 2282 37 4195 734.8 0 4195 38 5412 734.8 0 5412 39 126737 734.8 0 126737 40 103892 734.8 0 103892 41 27513 734.8 0 27513 42 276 734.8 0 276 43 126 734.8 0 126 44 164 734.8 0 164 45 318 734.8 0 318 46 940 734.8 0 940 47 192 734.8 0 192 48 347 734.8 0 347 49 480 734.8 0 480 50 286 734.8 0 286 51 333 734.8 0 333 52 558 734.8 0 558 53 445 734.8 0 445 54 795 734.8 0 795 55 712 734.8 0 712 56 1032 734.8 0 1032 57 1176 734.8 0 1176 58 2371 734.8 0 2371 59 57132 734.8 0 57132 60 77852 734.8 0 77852 61 12907 734.8 0 12907 62 137 734.8 0 137 63 15 734.8 0 15 64 12 734.8 0 12 65 5 734.8 0 5 66 4 734.8 0 4 67 4 734.8 0 4 68 11 734.8 0 11 69 7 734.8 0 7 70 8 734.8 0 8 71 12 734.8 0 12 72 65 734.8 0 65 73 28 734.8 0 28 74 123 734.8 0 123 75 10 734.8 0 10 76 5 734.8 0 5 77 6 734.8 0 6 78 8 734.8 0 8 79 17 734.8 0 17 80 14 734.8 0 14 81 36 734.8 0 36 82 86 734.8 0 86 83 25 734.8 0 25 84 25 734.8 0 25 85 19 734.8 0 19 86 26 734.8 0 26 87 18 734.8 0 18 88 19 734.8 0 19 89 12 734.8 0 12 90 61 734.8 0 61 91 20 734.8 0 20 92 21 734.8 0 21 93 14 734.8 0 14 94 21 734.8 0 21 95 15 734.8 0 15 96 16 734.8 0 16 97 24 734.8 0 24 98 21 734.8 0 21 99 49 734.8 0 49 100 33 734.8 0 33 101 409 734.8 0 409 102 469 734.8 0 469 103 98 734.8 0 98 104 7 734.8 0 7 105 9 734.8 0 9 106 9 734.8 0 9 107 8 734.8 0 8 108 7 734.8 0 7 109 11 734.8 0 11 110 23 734.8 0 23 111 149 734.8 0 149 112 441 734.8 0 441 113 52 734.8 0 52 114 14 734.8 0 14 115 14 734.8 0 14 116 22 734.8 0 22 117 62 734.8 0 62 118 43 734.8 0 43 119 26 734.8 0 26 120 21 734.8 0 21 121 22 734.8 0 22 122 19 734.8 0 19 123 27 734.8 0 27 124 36 734.8 0 36 125 29 734.8 0 29 126 20 734.8 0 20 127 15 734.8 0 15 128 8 734.8 0 8 129 37 734.8 0 37 130 55 734.8 0 55 131 47 734.8 0 47 132 48 734.8 0 48 133 32 734.8 0 32 134 27 734.8 0 27 135 12 734.8 0 12 136 31 734.8 0 31 137 30 734.8 0 30 138 33 734.8 0 33 139 24 734.8 0 24 140 10 734.8 0 10 141 30 734.8 0 30 142 27 734.8 0 27 143 38 734.8 0 38 144 38 734.8 0 38 145 34 734.8 0 34 146 18 734.8 0 18 147 42 734.8 0 42 148 35 734.8 0 35 149 40 734.8 0 40 150 26 734.8 0 26 151 20 734.8 0 20 152 26 734.8 0 26 153 17 734.8 0 17 154 26 734.8 0 26 155 31 734.8 0 31 156 53 734.8 0 53 157 48 734.8 0 48 158 56 734.8 0 56 159 37 734.8 0 37 160 24 734.8 0 24 161 87 734.8 0 87 162 56 734.8 0 56 163 71 734.8 0 71 164 37 734.8 0 37 165 43 734.8 0 43 166 28 734.8 0 28 167 55 734.8 0 55 168 67 734.8 0 67 169 532 734.8 0 532 170 892 734.8 0 892 171 108 734.8 0 108 172 25 734.8 0 25 173 23 734.8 0 23 174 28 734.8 0 28 175 20 734.8 0 20 176 62 734.8 0 62 177 64 734.8 0 64 178 70 734.8 0 70 179 62 734.8 0 62 180 25 734.8 0 25 181 31 734.8 0 31 182 31 734.8 0 31 183 39 734.8 0 39 184 76 734.8 0 76 185 69 734.8 0 69 186 72 734.8 0 72 187 105 734.8 0 105 188 91 734.8 0 91 189 52 734.8 0 52 190 23 734.8 0 23 191 12 734.8 0 12 192 20 734.8 0 20 193 23 734.8 0 23 194 29 734.8 0 29 195 36 734.8 0 36 196 76 734.8 0 76 197 78 734.8 0 78 198 328 734.8 0 328 199 408 734.8 0 408 200 188 734.8 0 188 201 68 734.8 0 68 202 56 734.8 0 56 203 99 734.8 0 99 204 134 734.8 0 134 205 100 734.8 0 100 206 44 734.8 0 44 207 40 734.8 0 40 208 57 734.8 0 57 209 37 734.8 0 37 210 33 734.8 0 33 211 33 734.8 0 33 212 54 734.8 0 54 213 56 734.8 0 56 214 58 734.8 0 58 215 43 734.8 0 43 216 49 734.8 0 49 217 26 734.8 0 26 218 47 734.8 0 47 219 42 734.8 0 42 220 48 734.8 0 48 221 60 734.8 0 60 222 70 734.8 0 70 223 516 734.8 0 516 224 334 734.8 0 334 225 173 734.8 0 173 226 413 734.8 0 413 227 191 734.8 0 191 228 184 734.8 0 184 229 164 734.8 0 164 230 37 734.8 0 37 231 17 734.8 0 17 232 18 734.8 0 18 233 26 734.8 0 26 234 40 734.8 0 40 235 18 734.8 0 18 236 17 734.8 0 17 237 45 734.8 0 45 238 37 734.8 0 37 239 24 734.8 0 24 240 18 734.8 0 18 241 25 734.8 0 25 242 34 734.8 0 34 243 45 734.8 0 45 244 49 734.8 0 49 245 32 734.8 0 32 246 22 734.8 0 22 247 37 734.8 0 37 248 40 734.8 0 40 249 26 734.8 0 26 250 26 734.8 0 26 251 39 734.8 0 39 252 19 734.8 0 19 253 21 734.8 0 21 254 42 734.8 0 42 255 59 734.8 0 59 256 70 734.8 0 70 257 56 734.8 0 56 258 35 734.8 0 35 259 23 734.8 0 23 260 21 734.8 0 21 261 19 734.8 0 19 262 19 734.8 0 19 263 62 734.8 0 62 264 57 734.8 0 57 265 35 734.8 0 35 266 28 734.8 0 28 267 18 734.8 0 18 268 14 734.8 0 14 269 35 734.8 0 35 270 41 734.8 0 41 271 156 734.8 0 156 272 103 734.8 0 103 273 160 734.8 0 160 274 64 734.8 0 64 275 102 734.8 0 102 276 61 734.8 0 61 277 38 734.8 0 38 278 19 734.8 0 19 279 16 734.8 0 16 280 26 734.8 0 26 281 32 734.8 0 32 282 34 734.8 0 34 283 33 734.8 0 33 284 32 734.8 0 32 285 60 734.8 0 60 286 41 734.8 0 41 287 19 734.8 0 19 288 28 734.8 0 28 289 31 734.8 0 31 290 46 734.8 0 46 291 52 734.8 0 52 292 31 734.8 0 31 293 26 734.8 0 26 294 53 734.8 0 53 295 40 734.8 0 40 296 31 734.8 0 31 297 38 734.8 0 38 298 25 734.8 0 25 299 36 734.8 0 36 300 21 734.8 0 21 301 26 734.8 0 26 Finished in 17.71 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 451,933 Reads with adapters: 8,248 (1.8%) Reads that were too short: 261 (0.1%) Reads written (passing filters): 7,987 (1.8%) Total basepairs processed: 114,505,930 bp Total written (filtered): 2,152,997 bp (1.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8248 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 38 0.0 3 0 33 4 1 17 7559 0.0 3 7364 167 24 4 18 18 0.0 3 3 14 1 19 5 0.0 3 0 0 3 2 20 7 0.0 3 3 0 0 4 22 2 0.0 3 0 0 0 2 24 3 0.0 3 1 0 0 2 29 1 0.0 3 1 33 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 48 1 0.0 3 1 50 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 9 0.0 3 0 0 0 9 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 1 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 107 4 0.0 3 0 0 0 4 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 6 0.0 3 0 0 0 6 131 7 0.0 3 0 0 0 7 132 94 0.0 3 0 0 0 94 133 28 0.0 3 0 0 0 28 134 7 0.0 3 0 0 0 7 135 34 0.0 3 0 0 1 33 136 12 0.0 3 0 0 0 12 150 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 158 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 128 0.0 3 0 0 0 128 176 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 9 0.0 3 0 0 0 9 188 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 206 3 0.0 3 0 0 0 3 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 3 0.0 3 0 0 1 2 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 233 5 0.0 3 0 0 1 4 234 76 0.0 3 0 0 4 72 235 3 0.0 3 0 0 0 3 246 1 0.0 3 0 0 0 1 247 4 0.0 3 0 0 0 4 248 1 0.0 3 0 0 0 1 249 53 0.0 3 0 0 1 52 250 48 0.0 3 0 0 1 47 252 2 0.0 3 0 0 0 2 253 2 0.0 3 0 0 0 2 256 17 0.0 3 0 0 0 17 258 2 0.0 3 0 0 0 2 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 277 2 0.0 3 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 7,987 Reads with adapters: 460 (5.8%) Reads written (passing filters): 7,987 (100.0%) Total basepairs processed: 2,152,997 bp Total written (filtered): 2,151,606 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 460 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.0% C: 41.1% G: 7.8% T: 4.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 452 124.8 0 452 4 7 31.2 0 7 7 1 0.5 0 1