This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.20 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 111,652 (3.7%) Reads that were too short: 63,722 (2.1%) Reads written (passing filters): 47,930 (1.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 13,259,346 bp (1.5%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 111652 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3545 734.8 0 3545 7 26374 734.8 0 26374 8 7970 734.8 0 7970 9 22 734.8 0 22 10 19 734.8 0 19 11 12 734.8 0 12 12 13 734.8 0 13 13 18 734.8 0 18 14 9 734.8 0 9 15 27 734.8 0 27 16 13 734.8 0 13 17 20 734.8 0 20 18 12 734.8 0 12 19 26 734.8 0 26 20 110 734.8 0 110 21 14 734.8 0 14 22 11 734.8 0 11 23 14 734.8 0 14 24 11 734.8 0 11 25 13 734.8 0 13 26 13 734.8 0 13 27 13 734.8 0 13 28 11 734.8 0 11 29 13 734.8 0 13 30 10 734.8 0 10 31 14 734.8 0 14 32 18 734.8 0 18 33 21 734.8 0 21 34 12 734.8 0 12 35 16 734.8 0 16 36 16 734.8 0 16 37 65 734.8 0 65 38 18 734.8 0 18 39 20 734.8 0 20 40 13 734.8 0 13 41 14 734.8 0 14 42 26 734.8 0 26 43 12 734.8 0 12 44 20 734.8 0 20 45 13 734.8 0 13 46 14 734.8 0 14 47 18 734.8 0 18 48 35 734.8 0 35 49 8 734.8 0 8 50 159 734.8 0 159 51 33 734.8 0 33 52 29 734.8 0 29 53 16 734.8 0 16 54 24 734.8 0 24 55 43 734.8 0 43 56 22 734.8 0 22 57 27 734.8 0 27 58 28 734.8 0 28 59 41 734.8 0 41 60 44 734.8 0 44 61 26 734.8 0 26 62 72 734.8 0 72 63 27 734.8 0 27 64 51 734.8 0 51 65 46 734.8 0 46 66 29 734.8 0 29 67 21 734.8 0 21 68 39 734.8 0 39 69 37 734.8 0 37 70 37 734.8 0 37 71 28 734.8 0 28 72 54 734.8 0 54 73 51 734.8 0 51 74 93 734.8 0 93 75 2705 734.8 0 2705 76 2054 734.8 0 2054 77 789 734.8 0 789 78 19 734.8 0 19 79 14 734.8 0 14 80 11 734.8 0 11 81 16 734.8 0 16 82 9 734.8 0 9 83 8 734.8 0 8 84 16 734.8 0 16 85 14 734.8 0 14 86 9 734.8 0 9 87 12 734.8 0 12 88 20 734.8 0 20 89 17 734.8 0 17 90 10 734.8 0 10 91 35 734.8 0 35 92 12 734.8 0 12 93 3 734.8 0 3 94 12 734.8 0 12 95 26 734.8 0 26 96 17 734.8 0 17 97 8 734.8 0 8 98 17 734.8 0 17 99 13 734.8 0 13 100 11 734.8 0 11 101 12 734.8 0 12 102 16 734.8 0 16 103 22 734.8 0 22 104 16 734.8 0 16 105 22 734.8 0 22 106 8 734.8 0 8 107 11 734.8 0 11 108 15 734.8 0 15 109 11 734.8 0 11 110 18 734.8 0 18 111 10 734.8 0 10 112 15 734.8 0 15 113 17 734.8 0 17 114 11 734.8 0 11 115 14 734.8 0 14 116 20 734.8 0 20 117 21 734.8 0 21 118 11 734.8 0 11 119 10 734.8 0 10 120 7 734.8 0 7 121 14 734.8 0 14 122 13 734.8 0 13 123 21 734.8 0 21 124 17 734.8 0 17 125 228 734.8 0 228 126 18 734.8 0 18 127 18 734.8 0 18 128 24 734.8 0 24 129 15 734.8 0 15 130 16 734.8 0 16 131 13 734.8 0 13 132 19 734.8 0 19 133 29 734.8 0 29 134 30 734.8 0 30 135 15 734.8 0 15 136 23 734.8 0 23 137 30 734.8 0 30 138 12 734.8 0 12 139 8 734.8 0 8 140 16 734.8 0 16 141 14 734.8 0 14 142 22 734.8 0 22 143 18 734.8 0 18 144 14 734.8 0 14 145 17 734.8 0 17 146 13 734.8 0 13 147 14 734.8 0 14 148 11 734.8 0 11 149 12 734.8 0 12 150 22 734.8 0 22 151 13 734.8 0 13 152 8 734.8 0 8 153 19 734.8 0 19 154 16 734.8 0 16 155 15 734.8 0 15 156 8 734.8 0 8 157 17 734.8 0 17 158 18 734.8 0 18 159 11 734.8 0 11 160 18 734.8 0 18 161 33 734.8 0 33 162 17 734.8 0 17 163 11 734.8 0 11 164 22 734.8 0 22 165 13 734.8 0 13 166 22 734.8 0 22 167 25 734.8 0 25 168 22 734.8 0 22 169 23 734.8 0 23 170 27 734.8 0 27 171 9 734.8 0 9 172 13 734.8 0 13 173 19 734.8 0 19 174 10 734.8 0 10 175 12 734.8 0 12 176 13 734.8 0 13 177 19 734.8 0 19 178 14 734.8 0 14 179 19 734.8 0 19 180 27 734.8 0 27 181 28 734.8 0 28 182 22 734.8 0 22 183 35 734.8 0 35 184 16 734.8 0 16 185 21 734.8 0 21 186 29 734.8 0 29 187 28 734.8 0 28 188 25 734.8 0 25 189 19 734.8 0 19 190 18 734.8 0 18 191 15 734.8 0 15 192 28 734.8 0 28 193 77 734.8 0 77 194 48 734.8 0 48 195 35 734.8 0 35 196 29 734.8 0 29 197 124 734.8 0 124 198 62 734.8 0 62 199 50 734.8 0 50 200 22 734.8 0 22 201 23 734.8 0 23 202 18 734.8 0 18 203 36 734.8 0 36 204 36 734.8 0 36 205 28 734.8 0 28 206 14 734.8 0 14 207 14 734.8 0 14 208 18 734.8 0 18 209 12 734.8 0 12 210 24 734.8 0 24 211 38 734.8 0 38 212 23 734.8 0 23 213 22 734.8 0 22 214 30 734.8 0 30 215 23 734.8 0 23 216 28 734.8 0 28 217 49 734.8 0 49 218 76 734.8 0 76 219 58 734.8 0 58 220 39 734.8 0 39 221 54 734.8 0 54 222 33 734.8 0 33 223 273 734.8 0 273 224 178 734.8 0 178 225 153 734.8 0 153 226 57 734.8 0 57 227 82 734.8 0 82 228 47 734.8 0 47 229 14 734.8 0 14 230 29 734.8 0 29 231 61 734.8 0 61 232 62 734.8 0 62 233 113 734.8 0 113 234 151 734.8 0 151 235 270 734.8 0 270 236 330 734.8 0 330 237 420 734.8 0 420 238 5543 734.8 0 5543 239 16253 734.8 0 16253 240 1974 734.8 0 1974 241 208 734.8 0 208 242 164 734.8 0 164 243 138 734.8 0 138 244 183 734.8 0 183 245 165 734.8 0 165 246 215 734.8 0 215 247 339 734.8 0 339 248 2772 734.8 0 2772 249 10227 734.8 0 10227 250 14834 734.8 0 14834 251 1182 734.8 0 1182 252 21 734.8 0 21 253 18 734.8 0 18 254 23 734.8 0 23 255 21 734.8 0 21 256 15 734.8 0 15 257 13 734.8 0 13 258 9 734.8 0 9 259 34 734.8 0 34 260 86 734.8 0 86 261 18 734.8 0 18 262 26 734.8 0 26 263 19 734.8 0 19 264 20 734.8 0 20 265 27 734.8 0 27 266 20 734.8 0 20 267 16 734.8 0 16 268 13 734.8 0 13 269 33 734.8 0 33 270 13 734.8 0 13 271 32 734.8 0 32 272 25 734.8 0 25 273 20 734.8 0 20 274 22 734.8 0 22 275 24 734.8 0 24 276 23 734.8 0 23 277 32 734.8 0 32 278 114 734.8 0 114 279 454 734.8 0 454 280 350 734.8 0 350 281 94 734.8 0 94 282 1427 734.8 0 1427 283 2313 734.8 0 2313 284 347 734.8 0 347 285 39 734.8 0 39 286 34 734.8 0 34 287 68 734.8 0 68 288 21 734.8 0 21 289 7 734.8 0 7 290 16 734.8 0 16 291 18 734.8 0 18 292 9 734.8 0 9 293 19 734.8 0 19 294 186 734.8 0 186 295 146 734.8 0 146 296 121 734.8 0 121 297 33 734.8 0 33 298 10 734.8 0 10 299 34 734.8 0 34 300 42 734.8 0 42 301 87 734.8 0 87 Finished in 2.51 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 47,930 Reads with adapters: 11,499 (24.0%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 11,498 (24.0%) Total basepairs processed: 13,259,346 bp Total written (filtered): 3,113,318 bp (23.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11499 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 10 0.0 3 0 0 10 19 100 0.0 3 0 97 2 1 20 8024 0.0 4 7933 83 6 0 2 21 63 0.0 4 11 52 22 3029 0.0 4 2992 37 23 16 0.0 4 3 7 0 5 1 24 2 0.0 4 0 0 2 25 2 0.0 4 1 0 0 1 58 1 0.0 4 0 0 0 0 1 59 18 0.0 4 0 0 0 0 18 85 1 0.0 4 0 0 0 0 1 111 31 0.0 4 0 0 0 29 2 113 2 0.0 4 0 0 0 2 122 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 194 0.0 4 0 0 0 0 194 128 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 11,498 Reads with adapters: 877 (7.6%) Reads written (passing filters): 11,498 (100.0%) Total basepairs processed: 3,113,318 bp Total written (filtered): 2,981,625 bp (95.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 877 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 54.4% C: 1.8% G: 43.6% T: 0.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 179.7 0 1 6 1 2.8 0 1 9 1 0.0 0 0 1 10 112 0.0 1 3 109 11 270 0.0 1 1 269 237 1 0.0 1 1 258 1 0.0 1 1 259 414 0.0 1 402 12 260 23 0.0 1 21 2 261 18 0.0 1 17 1 262 29 0.0 1 28 1 263 1 0.0 1 1 264 1 0.0 1 1 265 3 0.0 1 3 266 1 0.0 1 1